doxycycline Search Results


doxycycline hydrochloride merck  (Thermo Fisher)
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Thermo Fisher doxycycline hydrochloride merck
Doxycycline Hydrochloride Merck, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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intraperitoneal i p injection  (Echelon Biosciences)
94
Echelon Biosciences intraperitoneal i p injection
Intraperitoneal I P Injection, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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doxycycline hyclate  (Selleck Chemicals)
93
Selleck Chemicals doxycycline hyclate
Doxycycline Hyclate, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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doxycycline  (Selleck Chemicals)
96
Selleck Chemicals doxycycline
Figure 4. Overexpression of MESP1 variants at days 2 to 4 post hESC differentiation impaired cardiomyocyte differentiation (A) Graph showing the timescale of Dox-induced FLAG-MESP1 overexpression during hESCs cardiac-directed differentiation. Dox was added from days 2 to 4. (B) RT-qPCR from day 30 cardiomyocytes with MESP1 or MESP1 variants (E11D, E104K, or L120P) induced at the cardiac mesoderm stage. Relative expression of MESP1 (at day 4) induced by varying <t>doxycycline</t> concentrations (mg/mL) was shown on the right side. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. (C) Immunostaining and quantification of irregular organized sarcomeric a-ACTININ in day 30 cardiomyocytes with or without MESP1 (WT, E11D, E104K, or L120P) overexpression induced by Dox at the cardiac mesoderm stage. n = 96 (OE MESP1 Dox), n = 38 (OE MESP1 + Dox 0.5 mg/mL, 26-fold overexpression), n = 14 (OE MESP1 E11D Dox), n = 25 (OE MESP1 E11D + Dox 0.5 mg/mL, 40-fold overexpression), n = 27 (OE MESP1 E104K Dox), n = 52 (OE MESP1 E104K + Dox 0.1 mg/mL, 5-fold overexpression), n = 34 (OE MESP1 L120P Dox), and n = 33 (OE MESP1 L120P + Dox 0.2 mg/mL, 17-fold overexpression). Scale bars, 50 mm. ns, p > 0.05, ***p < 0.001 by Fisher’s exact test.
Doxycycline, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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doxycycline hyclate  (Thermo Fisher)
96
Thermo Fisher doxycycline hyclate
Figure 4. Overexpression of MESP1 variants at days 2 to 4 post hESC differentiation impaired cardiomyocyte differentiation (A) Graph showing the timescale of Dox-induced FLAG-MESP1 overexpression during hESCs cardiac-directed differentiation. Dox was added from days 2 to 4. (B) RT-qPCR from day 30 cardiomyocytes with MESP1 or MESP1 variants (E11D, E104K, or L120P) induced at the cardiac mesoderm stage. Relative expression of MESP1 (at day 4) induced by varying <t>doxycycline</t> concentrations (mg/mL) was shown on the right side. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. (C) Immunostaining and quantification of irregular organized sarcomeric a-ACTININ in day 30 cardiomyocytes with or without MESP1 (WT, E11D, E104K, or L120P) overexpression induced by Dox at the cardiac mesoderm stage. n = 96 (OE MESP1 Dox), n = 38 (OE MESP1 + Dox 0.5 mg/mL, 26-fold overexpression), n = 14 (OE MESP1 E11D Dox), n = 25 (OE MESP1 E11D + Dox 0.5 mg/mL, 40-fold overexpression), n = 27 (OE MESP1 E104K Dox), n = 52 (OE MESP1 E104K + Dox 0.1 mg/mL, 5-fold overexpression), n = 34 (OE MESP1 L120P Dox), and n = 33 (OE MESP1 L120P + Dox 0.2 mg/mL, 17-fold overexpression). Scale bars, 50 mm. ns, p > 0.05, ***p < 0.001 by Fisher’s exact test.
Doxycycline Hyclate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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doxycycline dox  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology doxycycline dox
Figure 4. Overexpression of MESP1 variants at days 2 to 4 post hESC differentiation impaired cardiomyocyte differentiation (A) Graph showing the timescale of Dox-induced FLAG-MESP1 overexpression during hESCs cardiac-directed differentiation. Dox was added from days 2 to 4. (B) RT-qPCR from day 30 cardiomyocytes with MESP1 or MESP1 variants (E11D, E104K, or L120P) induced at the cardiac mesoderm stage. Relative expression of MESP1 (at day 4) induced by varying <t>doxycycline</t> concentrations (mg/mL) was shown on the right side. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. (C) Immunostaining and quantification of irregular organized sarcomeric a-ACTININ in day 30 cardiomyocytes with or without MESP1 (WT, E11D, E104K, or L120P) overexpression induced by Dox at the cardiac mesoderm stage. n = 96 (OE MESP1 Dox), n = 38 (OE MESP1 + Dox 0.5 mg/mL, 26-fold overexpression), n = 14 (OE MESP1 E11D Dox), n = 25 (OE MESP1 E11D + Dox 0.5 mg/mL, 40-fold overexpression), n = 27 (OE MESP1 E104K Dox), n = 52 (OE MESP1 E104K + Dox 0.1 mg/mL, 5-fold overexpression), n = 34 (OE MESP1 L120P Dox), and n = 33 (OE MESP1 L120P + Dox 0.2 mg/mL, 17-fold overexpression). Scale bars, 50 mm. ns, p > 0.05, ***p < 0.001 by Fisher’s exact test.
Doxycycline Dox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dox  (Tocris)
95
Tocris dox
Figure 4. Overexpression of MESP1 variants at days 2 to 4 post hESC differentiation impaired cardiomyocyte differentiation (A) Graph showing the timescale of Dox-induced FLAG-MESP1 overexpression during hESCs cardiac-directed differentiation. Dox was added from days 2 to 4. (B) RT-qPCR from day 30 cardiomyocytes with MESP1 or MESP1 variants (E11D, E104K, or L120P) induced at the cardiac mesoderm stage. Relative expression of MESP1 (at day 4) induced by varying <t>doxycycline</t> concentrations (mg/mL) was shown on the right side. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. (C) Immunostaining and quantification of irregular organized sarcomeric a-ACTININ in day 30 cardiomyocytes with or without MESP1 (WT, E11D, E104K, or L120P) overexpression induced by Dox at the cardiac mesoderm stage. n = 96 (OE MESP1 Dox), n = 38 (OE MESP1 + Dox 0.5 mg/mL, 26-fold overexpression), n = 14 (OE MESP1 E11D Dox), n = 25 (OE MESP1 E11D + Dox 0.5 mg/mL, 40-fold overexpression), n = 27 (OE MESP1 E104K Dox), n = 52 (OE MESP1 E104K + Dox 0.1 mg/mL, 5-fold overexpression), n = 34 (OE MESP1 L120P Dox), and n = 33 (OE MESP1 L120P + Dox 0.2 mg/mL, 17-fold overexpression). Scale bars, 50 mm. ns, p > 0.05, ***p < 0.001 by Fisher’s exact test.
Dox, supplied by Tocris, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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doxycyclin hyclate  (LKT Laboratories)
93
LKT Laboratories doxycyclin hyclate
Figure 4. Overexpression of MESP1 variants at days 2 to 4 post hESC differentiation impaired cardiomyocyte differentiation (A) Graph showing the timescale of Dox-induced FLAG-MESP1 overexpression during hESCs cardiac-directed differentiation. Dox was added from days 2 to 4. (B) RT-qPCR from day 30 cardiomyocytes with MESP1 or MESP1 variants (E11D, E104K, or L120P) induced at the cardiac mesoderm stage. Relative expression of MESP1 (at day 4) induced by varying <t>doxycycline</t> concentrations (mg/mL) was shown on the right side. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. (C) Immunostaining and quantification of irregular organized sarcomeric a-ACTININ in day 30 cardiomyocytes with or without MESP1 (WT, E11D, E104K, or L120P) overexpression induced by Dox at the cardiac mesoderm stage. n = 96 (OE MESP1 Dox), n = 38 (OE MESP1 + Dox 0.5 mg/mL, 26-fold overexpression), n = 14 (OE MESP1 E11D Dox), n = 25 (OE MESP1 E11D + Dox 0.5 mg/mL, 40-fold overexpression), n = 27 (OE MESP1 E104K Dox), n = 52 (OE MESP1 E104K + Dox 0.1 mg/mL, 5-fold overexpression), n = 34 (OE MESP1 L120P Dox), and n = 33 (OE MESP1 L120P + Dox 0.2 mg/mL, 17-fold overexpression). Scale bars, 50 mm. ns, p > 0.05, ***p < 0.001 by Fisher’s exact test.
Doxycyclin Hyclate, supplied by LKT Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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doxycycline  (Thermo Fisher)
94
Thermo Fisher doxycycline
Figure 4. Overexpression of MESP1 variants at days 2 to 4 post hESC differentiation impaired cardiomyocyte differentiation (A) Graph showing the timescale of Dox-induced FLAG-MESP1 overexpression during hESCs cardiac-directed differentiation. Dox was added from days 2 to 4. (B) RT-qPCR from day 30 cardiomyocytes with MESP1 or MESP1 variants (E11D, E104K, or L120P) induced at the cardiac mesoderm stage. Relative expression of MESP1 (at day 4) induced by varying <t>doxycycline</t> concentrations (mg/mL) was shown on the right side. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. (C) Immunostaining and quantification of irregular organized sarcomeric a-ACTININ in day 30 cardiomyocytes with or without MESP1 (WT, E11D, E104K, or L120P) overexpression induced by Dox at the cardiac mesoderm stage. n = 96 (OE MESP1 Dox), n = 38 (OE MESP1 + Dox 0.5 mg/mL, 26-fold overexpression), n = 14 (OE MESP1 E11D Dox), n = 25 (OE MESP1 E11D + Dox 0.5 mg/mL, 40-fold overexpression), n = 27 (OE MESP1 E104K Dox), n = 52 (OE MESP1 E104K + Dox 0.1 mg/mL, 5-fold overexpression), n = 34 (OE MESP1 L120P Dox), and n = 33 (OE MESP1 L120P + Dox 0.2 mg/mL, 17-fold overexpression). Scale bars, 50 mm. ns, p > 0.05, ***p < 0.001 by Fisher’s exact test.
Doxycycline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ac446061000  (Thermo Fisher)
94
Thermo Fisher ac446061000
Figure 4. Overexpression of MESP1 variants at days 2 to 4 post hESC differentiation impaired cardiomyocyte differentiation (A) Graph showing the timescale of Dox-induced FLAG-MESP1 overexpression during hESCs cardiac-directed differentiation. Dox was added from days 2 to 4. (B) RT-qPCR from day 30 cardiomyocytes with MESP1 or MESP1 variants (E11D, E104K, or L120P) induced at the cardiac mesoderm stage. Relative expression of MESP1 (at day 4) induced by varying <t>doxycycline</t> concentrations (mg/mL) was shown on the right side. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. (C) Immunostaining and quantification of irregular organized sarcomeric a-ACTININ in day 30 cardiomyocytes with or without MESP1 (WT, E11D, E104K, or L120P) overexpression induced by Dox at the cardiac mesoderm stage. n = 96 (OE MESP1 Dox), n = 38 (OE MESP1 + Dox 0.5 mg/mL, 26-fold overexpression), n = 14 (OE MESP1 E11D Dox), n = 25 (OE MESP1 E11D + Dox 0.5 mg/mL, 40-fold overexpression), n = 27 (OE MESP1 E104K Dox), n = 52 (OE MESP1 E104K + Dox 0.1 mg/mL, 5-fold overexpression), n = 34 (OE MESP1 L120P Dox), and n = 33 (OE MESP1 L120P + Dox 0.2 mg/mL, 17-fold overexpression). Scale bars, 50 mm. ns, p > 0.05, ***p < 0.001 by Fisher’s exact test.
Ac446061000, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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doxycycline hyclate  (Tocris)
95
Tocris doxycycline hyclate
Figure 4. Overexpression of MESP1 variants at days 2 to 4 post hESC differentiation impaired cardiomyocyte differentiation (A) Graph showing the timescale of Dox-induced FLAG-MESP1 overexpression during hESCs cardiac-directed differentiation. Dox was added from days 2 to 4. (B) RT-qPCR from day 30 cardiomyocytes with MESP1 or MESP1 variants (E11D, E104K, or L120P) induced at the cardiac mesoderm stage. Relative expression of MESP1 (at day 4) induced by varying <t>doxycycline</t> concentrations (mg/mL) was shown on the right side. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. (C) Immunostaining and quantification of irregular organized sarcomeric a-ACTININ in day 30 cardiomyocytes with or without MESP1 (WT, E11D, E104K, or L120P) overexpression induced by Dox at the cardiac mesoderm stage. n = 96 (OE MESP1 Dox), n = 38 (OE MESP1 + Dox 0.5 mg/mL, 26-fold overexpression), n = 14 (OE MESP1 E11D Dox), n = 25 (OE MESP1 E11D + Dox 0.5 mg/mL, 40-fold overexpression), n = 27 (OE MESP1 E104K Dox), n = 52 (OE MESP1 E104K + Dox 0.1 mg/mL, 5-fold overexpression), n = 34 (OE MESP1 L120P Dox), and n = 33 (OE MESP1 L120P + Dox 0.2 mg/mL, 17-fold overexpression). Scale bars, 50 mm. ns, p > 0.05, ***p < 0.001 by Fisher’s exact test.
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doxycycline d3 hyclate  (Toronto Research Chemicals)
91
Toronto Research Chemicals doxycycline d3 hyclate
Figure 4. Overexpression of MESP1 variants at days 2 to 4 post hESC differentiation impaired cardiomyocyte differentiation (A) Graph showing the timescale of Dox-induced FLAG-MESP1 overexpression during hESCs cardiac-directed differentiation. Dox was added from days 2 to 4. (B) RT-qPCR from day 30 cardiomyocytes with MESP1 or MESP1 variants (E11D, E104K, or L120P) induced at the cardiac mesoderm stage. Relative expression of MESP1 (at day 4) induced by varying <t>doxycycline</t> concentrations (mg/mL) was shown on the right side. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. (C) Immunostaining and quantification of irregular organized sarcomeric a-ACTININ in day 30 cardiomyocytes with or without MESP1 (WT, E11D, E104K, or L120P) overexpression induced by Dox at the cardiac mesoderm stage. n = 96 (OE MESP1 Dox), n = 38 (OE MESP1 + Dox 0.5 mg/mL, 26-fold overexpression), n = 14 (OE MESP1 E11D Dox), n = 25 (OE MESP1 E11D + Dox 0.5 mg/mL, 40-fold overexpression), n = 27 (OE MESP1 E104K Dox), n = 52 (OE MESP1 E104K + Dox 0.1 mg/mL, 5-fold overexpression), n = 34 (OE MESP1 L120P Dox), and n = 33 (OE MESP1 L120P + Dox 0.2 mg/mL, 17-fold overexpression). Scale bars, 50 mm. ns, p > 0.05, ***p < 0.001 by Fisher’s exact test.
Doxycycline D3 Hyclate, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 4. Overexpression of MESP1 variants at days 2 to 4 post hESC differentiation impaired cardiomyocyte differentiation (A) Graph showing the timescale of Dox-induced FLAG-MESP1 overexpression during hESCs cardiac-directed differentiation. Dox was added from days 2 to 4. (B) RT-qPCR from day 30 cardiomyocytes with MESP1 or MESP1 variants (E11D, E104K, or L120P) induced at the cardiac mesoderm stage. Relative expression of MESP1 (at day 4) induced by varying doxycycline concentrations (mg/mL) was shown on the right side. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. (C) Immunostaining and quantification of irregular organized sarcomeric a-ACTININ in day 30 cardiomyocytes with or without MESP1 (WT, E11D, E104K, or L120P) overexpression induced by Dox at the cardiac mesoderm stage. n = 96 (OE MESP1 Dox), n = 38 (OE MESP1 + Dox 0.5 mg/mL, 26-fold overexpression), n = 14 (OE MESP1 E11D Dox), n = 25 (OE MESP1 E11D + Dox 0.5 mg/mL, 40-fold overexpression), n = 27 (OE MESP1 E104K Dox), n = 52 (OE MESP1 E104K + Dox 0.1 mg/mL, 5-fold overexpression), n = 34 (OE MESP1 L120P Dox), and n = 33 (OE MESP1 L120P + Dox 0.2 mg/mL, 17-fold overexpression). Scale bars, 50 mm. ns, p > 0.05, ***p < 0.001 by Fisher’s exact test.

Journal: Developmental cell

Article Title: Essential role of MESP1-RING1A complex in cardiac differentiation.

doi: 10.1016/j.devcel.2022.10.009

Figure Lengend Snippet: Figure 4. Overexpression of MESP1 variants at days 2 to 4 post hESC differentiation impaired cardiomyocyte differentiation (A) Graph showing the timescale of Dox-induced FLAG-MESP1 overexpression during hESCs cardiac-directed differentiation. Dox was added from days 2 to 4. (B) RT-qPCR from day 30 cardiomyocytes with MESP1 or MESP1 variants (E11D, E104K, or L120P) induced at the cardiac mesoderm stage. Relative expression of MESP1 (at day 4) induced by varying doxycycline concentrations (mg/mL) was shown on the right side. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test. (C) Immunostaining and quantification of irregular organized sarcomeric a-ACTININ in day 30 cardiomyocytes with or without MESP1 (WT, E11D, E104K, or L120P) overexpression induced by Dox at the cardiac mesoderm stage. n = 96 (OE MESP1 Dox), n = 38 (OE MESP1 + Dox 0.5 mg/mL, 26-fold overexpression), n = 14 (OE MESP1 E11D Dox), n = 25 (OE MESP1 E11D + Dox 0.5 mg/mL, 40-fold overexpression), n = 27 (OE MESP1 E104K Dox), n = 52 (OE MESP1 E104K + Dox 0.1 mg/mL, 5-fold overexpression), n = 34 (OE MESP1 L120P Dox), and n = 33 (OE MESP1 L120P + Dox 0.2 mg/mL, 17-fold overexpression). Scale bars, 50 mm. ns, p > 0.05, ***p < 0.001 by Fisher’s exact test.

Article Snippet: For FLAG-MESP1 ChIP, cells with Dox-inducible FLAG-MESP1 were treated with 0.03 mg/ml doxycycline (Selleck Chemicals, Houston, TX) during day 3 - day 4 of the cardiomyocyte differentiation process.

Techniques: Over Expression, Quantitative RT-PCR, Expressing, Immunostaining