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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: In Silico and In Vitro Identification of P-Glycoprotein Inhibitors from a Library of 375 Phytochemicals
doi: 10.3390/ijms241210240
Figure Lengend Snippet: Dose–response curve for the nine selected compounds. ( A ) bidwillon A; ( B ) neobavaisoflavone; ( C ) coptisine chloride; ( D ) chamazulene; ( E ) miltirone; ( F ) z-guggulsterone. Shown are the dose–response curves of wildtype drug-sensitive CCRF-CEM cells and their P-gp-overexpressing multidrug-resistant subline CEM/ADR5000. Mean values ± SD of three independent experiments, each with six parallel measurements, are presented.
Article Snippet: D,E show the increased doxorubicin fluorescence in
Techniques:
Journal: International Journal of Molecular Sciences
Article Title: In Silico and In Vitro Identification of P-Glycoprotein Inhibitors from a Library of 375 Phytochemicals
doi: 10.3390/ijms241210240
Figure Lengend Snippet: Selection of six candidate compounds based on their interaction with P-gp (as determined by AutoDock and PyRx) and their structural alignment with known P-gp inhibitors (as determined by Torch3D). Scores > 0.5 were labeled in bold.
Article Snippet: D,E show the increased doxorubicin fluorescence in
Techniques: Selection, Labeling
Journal: International Journal of Molecular Sciences
Article Title: In Silico and In Vitro Identification of P-Glycoprotein Inhibitors from a Library of 375 Phytochemicals
doi: 10.3390/ijms241210240
Figure Lengend Snippet: Flow cytometric analysis of doxorubicin uptake by P-gp-overexpressing CEM/ADR5000 cells with and without coincubation of six phytochemical candidate compounds. The bar diagrams represent mean fluorescence intensity values of CEM/ADR5000 cells as percentages of the fluorescence in CCRF-CEM control cells. The bars show mean values ± SD of three independent experiments. ( A ) bidwillon A; ( B ) neobavaisoflavone; ( C ) coptisine chloride; ( D ) chamazulene; ( E ) miltirone; and ( F ) z-guggulsterone. * p < 0.05.
Article Snippet: D,E show the increased doxorubicin fluorescence in
Techniques: Fluorescence, Control
Journal: International Journal of Molecular Sciences
Article Title: In Silico and In Vitro Identification of P-Glycoprotein Inhibitors from a Library of 375 Phytochemicals
doi: 10.3390/ijms241210240
Figure Lengend Snippet: Molecular docking of bidwillon A and miltirone in relation to wildtype and mutated P-gp forms. ( A ) Missense mutations in the transmembrane domains of P-gp. ( B ) Molecular docking (defined mode) of bidwillon A and miltirone in relation to single, double, and quadruple P-gp mutants. Tariquidar and doxorubicin served as control drugs as the known inhibitor and the substrate, respectively. ( C ) Linear regressions of all binding energies and predicted inhibition constant (pKi) values in closed and open P-gp conformations ( r > 0.9).
Article Snippet: D,E show the increased doxorubicin fluorescence in
Techniques: Control, Binding Assay, Inhibition