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  • 94
    Millipore czapek dox broth
    Kinetic reduction of zearalenone during incubation experiments with isolates AN 154 ( C. rosea ), AN 169 ( C. catenulatum ) and AN 171 ( T. aggressivum ). Experiments were carried out at 25°C, in liquid <t>Czapek-Dox</t> medium supplemented with yeast extract and zearalenone.
    Czapek Dox Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Avanti Polar dox np
    UV–visible spectrum of a) <t>Dox-NP™</t> liposomes in PBS, b) DoxHCl in PBS at the same (40 μg/mL) <t>doxorubicin</t> concentration.
    Dox Np, supplied by Avanti Polar, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore czapek dox
    Photos of G2 in liquid media (top); from left to right: <t>Czapek</t> <t>dox,</t> 2% malt extract, potato dextrose, YPSS, YESD, and PYG broths. Photos of G2 on solid media (bottom); from left to right: rice, grits, oatmeal, wheat germ, and 3:1:1:1, respectively, of the same. Enlargements of the photos (2.5×) are provided in the lower right hand corner to help visualize the production of spores (orange) and/or exudates (black).
    Czapek Dox, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore dox treatment dox hyclate
    Photos of G2 in liquid media (top); from left to right: <t>Czapek</t> <t>dox,</t> 2% malt extract, potato dextrose, YPSS, YESD, and PYG broths. Photos of G2 on solid media (bottom); from left to right: rice, grits, oatmeal, wheat germ, and 3:1:1:1, respectively, of the same. Enlargements of the photos (2.5×) are provided in the lower right hand corner to help visualize the production of spores (orange) and/or exudates (black).
    Dox Treatment Dox Hyclate, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Pfizer Inc dox hydrochloride dox hcl
    Photos of G2 in liquid media (top); from left to right: <t>Czapek</t> <t>dox,</t> 2% malt extract, potato dextrose, YPSS, YESD, and PYG broths. Photos of G2 on solid media (bottom); from left to right: rice, grits, oatmeal, wheat germ, and 3:1:1:1, respectively, of the same. Enlargements of the photos (2.5×) are provided in the lower right hand corner to help visualize the production of spores (orange) and/or exudates (black).
    Dox Hydrochloride Dox Hcl, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore doxycycline dox
    Active XBP1s is not sufficient to induce GRASP55 expression. ( a ) Timeline of experiment with overexpression of <t>Dox-inducible</t> active transcription factors ATF6 and XBP1s. ( b , c ) <t>Lentiviral</t> transduction was used to express active transcription factors XBP1s and ATF6 in primary cortical neurons. XBP1s and ATF6 should only be expressed upon Dox addition (TetON construct). Timeline as represented in ( a ). mRNA expression levels were analyzed by qPCR of UPR target genes ( b ), GRASP55 and GRASP65 ( c ) upon Dox addition (1 µg/ml). Data are shown as fold change difference over the expression of a control construct with a similar backbone (TetON) in the presence of Dox (baseline, set to 1). Significant differences of n independent experiments ( n is shown in bars) were tested via one-way ANOVA followed by post hoc Dunnett’s multiple comparison test compared to baseline.
    Doxycycline Dox, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 736 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore dox
    Experimental design. ( A ) A schematic of the <t>HIV-1</t> and HIV-rtTA genome is shown, with the LTR subdivided in U3, R and U5 domains. In HIV-rtTA, the Tat-TAR axis of transcription regulation was inactivated by multiple nucleotide substitutions in the bulge and loop sequences of TAR (TAR mut , crossed boxes). Transcription and replication of the virus were made <t>dox</t> dependent by the introduction of tetO elements in the U3 region of the LTR promoter and replacement of the nef gene by the rtTA gene. The short TAR-only and full-length RNA molecules produced by non-processive (in the absence of Tat [-Tat] or dox [-dox]) and processive transcription (in the presence of Tat [+Tat] or dox [+dox]), respectively, are shown. ( B ) Structure of the TAR hairpin of HIV-1 (TAR wt ) and HIV-rtTA (TAR mut ). The bulge and loop mutations present in TAR mut are boxed in black. ( C ) Strategy used to identify AGO2-bound viral RNAs.
    Dox, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2762 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Shenzhen Main Luck Pharmaceuticals Inc chemicals dox
    Experimental design. ( A ) A schematic of the <t>HIV-1</t> and HIV-rtTA genome is shown, with the LTR subdivided in U3, R and U5 domains. In HIV-rtTA, the Tat-TAR axis of transcription regulation was inactivated by multiple nucleotide substitutions in the bulge and loop sequences of TAR (TAR mut , crossed boxes). Transcription and replication of the virus were made <t>dox</t> dependent by the introduction of tetO elements in the U3 region of the LTR promoter and replacement of the nef gene by the rtTA gene. The short TAR-only and full-length RNA molecules produced by non-processive (in the absence of Tat [-Tat] or dox [-dox]) and processive transcription (in the presence of Tat [+Tat] or dox [+dox]), respectively, are shown. ( B ) Structure of the TAR hairpin of HIV-1 (TAR wt ) and HIV-rtTA (TAR mut ). The bulge and loop mutations present in TAR mut are boxed in black. ( C ) Strategy used to identify AGO2-bound viral RNAs.
    Chemicals Dox, supplied by Shenzhen Main Luck Pharmaceuticals Inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Serv dox chow
    Transgene induction 4 weeks after transverse aortic constriction (TAC). (A) GLUT1 protein content in whole heart homogenates 2 days after <t>DOX</t> injection (mice were 10 weeks at the time of DOX injections). (B) Glucose uptake in isolated cardiomyocytes from Cont and <t>G1HA</t> hearts 2 days after DOX injection. Data are expressed as means±SEM. Significant differences were determined by Student's t ‐test. (*) vs Cont. Two‐way ANOVA was performed to analyze differences by treatment and genotype, including a Tukey post hoc analysis when significant interaction occurred. (C) Representative GLUT1 and GLUT4 immunoblot in Cont and G1HA hearts 4 weeks after TAC. (D) Densitometry of GLUT1 protein ( P
    Dox Chow, supplied by Bio-Serv, used in various techniques. Bioz Stars score: 94/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Envigo dox chow
    Ectopic PAR-1 activity restores KPC2 Par-1 KO cell tumor growth. A, Anti-Myc immunofluorescence of KPC2 Par-1 KO/Tg cells treated ± <t>Dox.</t> B, qRT-PCR for endogenous and Tg Par-1 transcripts comparing KPC2 WT and KPC2 Par-1 KO/Tg cells ± Dox stimulation (N = 3 per group). C, Immunoblot for phospho-ERK in KPC2 cells containing a <t>Tet-inducible</t> luciferase expression vector (KPC2 TetLuc ) and KPC2 Par-1 KO/Tg cells stimulated ± Dox and ± thrombin. D and E, Analysis of tumor volume and final tumor mass following subcutaneous injection of KPC2 WT cells or KPC2 Par-1 KO1 cells ± Dox ( N = 8 mice per group). F and G, Analysis of tumor volume and final tumor mass following subcutaneous injection of KPC2 Par-1 KO/Tg cells into cohorts of mice treated ± Dox ( N = 12 mice per group). Scale bars, 25 μm. **, P
    Dox Chow, supplied by Envigo, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore dox hydrochloride
    Schematic illustration of the stealth property and promoted tumor cell uptake of nanoconjugates ( A ) and <t>DOX-loaded</t> nanoconjugates (DOX/HDPEPM) ( B ). Notes: Depicted in ( A ), nanoconjugates minimize nonspecific interactions with serum components and change the surface charge of nanoconjugates in response to the tumor acidity (pH e ), leading to promoted cell internalization by the combination of electrostatic absorptive endocytosis and receptor-mediated endocytosis. Abbreviations: DMA, 2,3-dimethylmaleic anhydride; DOX, doxorubicin; HDPEPM, nanoconjugate formed by covalent attachment of fragment HAb18 F(ab′) 2 and 2,3-dimethylmaleic anhydride to polyethylenimine-modified poly(β-L-malic acid); <t>PEI,</t> polyethylenimine; PMLA, poly(β-L-malic acid).
    Dox Hydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Duchefa doxycyclin dox
    Schematic illustration of the stealth property and promoted tumor cell uptake of nanoconjugates ( A ) and <t>DOX-loaded</t> nanoconjugates (DOX/HDPEPM) ( B ). Notes: Depicted in ( A ), nanoconjugates minimize nonspecific interactions with serum components and change the surface charge of nanoconjugates in response to the tumor acidity (pH e ), leading to promoted cell internalization by the combination of electrostatic absorptive endocytosis and receptor-mediated endocytosis. Abbreviations: DMA, 2,3-dimethylmaleic anhydride; DOX, doxorubicin; HDPEPM, nanoconjugate formed by covalent attachment of fragment HAb18 F(ab′) 2 and 2,3-dimethylmaleic anhydride to polyethylenimine-modified poly(β-L-malic acid); <t>PEI,</t> polyethylenimine; PMLA, poly(β-L-malic acid).
    Doxycyclin Dox, supplied by Duchefa, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Nanjing KeyGen Biotech Co Ltd k562 dox
    Curcumin downregulates P-gp and S100A8 protein expression in <t>K562/DOX</t> cells. (A) K562/DOX cells were treated with curcumin (0.5, 1 or 2 µM) for 48 h, and western blot analysis was performed to detect the protein expression levels of (B) P-gp and (C) S100A8. (D) K562/DOX cells were treated with curcumin (2 µM) for 24, 48 or 72 h, followed by western blot analysis of (E) P-gp and (F) S100A8 protein expression. **P
    K562 Dox, supplied by Nanjing KeyGen Biotech Co Ltd, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Santa Cruz Biotechnology prepared dox
    Curcumin downregulates P-gp and S100A8 protein expression in <t>K562/DOX</t> cells. (A) K562/DOX cells were treated with curcumin (0.5, 1 or 2 µM) for 48 h, and western blot analysis was performed to detect the protein expression levels of (B) P-gp and (C) S100A8. (D) K562/DOX cells were treated with curcumin (2 µM) for 24, 48 or 72 h, followed by western blot analysis of (E) P-gp and (F) S100A8 protein expression. **P
    Prepared Dox, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    J&K Scientific dox hydrochloride
    Curcumin downregulates P-gp and S100A8 protein expression in <t>K562/DOX</t> cells. (A) K562/DOX cells were treated with curcumin (0.5, 1 or 2 µM) for 48 h, and western blot analysis was performed to detect the protein expression levels of (B) P-gp and (C) S100A8. (D) K562/DOX cells were treated with curcumin (2 µM) for 24, 48 or 72 h, followed by western blot analysis of (E) P-gp and (F) S100A8 protein expression. **P
    Dox Hydrochloride, supplied by J&K Scientific, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Tokyo Chemical Industry dox hydrochloride
    Curcumin downregulates P-gp and S100A8 protein expression in <t>K562/DOX</t> cells. (A) K562/DOX cells were treated with curcumin (0.5, 1 or 2 µM) for 48 h, and western blot analysis was performed to detect the protein expression levels of (B) P-gp and (C) S100A8. (D) K562/DOX cells were treated with curcumin (2 µM) for 24, 48 or 72 h, followed by western blot analysis of (E) P-gp and (F) S100A8 protein expression. **P
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    91
    Millipore dox powder
    VEGF + fat transplants improve glucose tolerance upon an <t>HFD</t> challenge. A : Schematic diagram of sWAT transplantation. Wild-type controls and VEGF-Tg mice were fed with a chow diet containing <t>dox</t> (60 mg/ kg) for 1 week. sWAT from either control mice or mice expressing VEGF + was harvested and subsequently implanted into C57/BL6J wild-type hosts. The recipient mice were fed with an HFD containing a low dose of dox (60 mg/ kg) for 3 weeks. B : Glucose levels were determined during an OGTT 3 weeks posttransplantion after continued HFD feeding. The difference at each time point was analyzed by Student t test. * P
    Dox Powder, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Kinetic reduction of zearalenone during incubation experiments with isolates AN 154 ( C. rosea ), AN 169 ( C. catenulatum ) and AN 171 ( T. aggressivum ). Experiments were carried out at 25°C, in liquid Czapek-Dox medium supplemented with yeast extract and zearalenone.

    Journal: BMC Microbiology

    Article Title: Zearalenone lactonohydrolase activity in Hypocreales and its evolutionary relationships within the epoxide hydrolase subset of a/b-hydrolases

    doi: 10.1186/1471-2180-14-82

    Figure Lengend Snippet: Kinetic reduction of zearalenone during incubation experiments with isolates AN 154 ( C. rosea ), AN 169 ( C. catenulatum ) and AN 171 ( T. aggressivum ). Experiments were carried out at 25°C, in liquid Czapek-Dox medium supplemented with yeast extract and zearalenone.

    Article Snippet: Sample preparation The fungal mycelium was grown in 50 ml Czapek-Dox broth (Sigma-Aldrich) with Yeast Extract (Oxoid) for 9 days at 25°C with rotary shaking at 100 rpm.

    Techniques: Incubation

    Relative normalized expression (N-fold) of zearalenone lactonohydrolase transcripts during incubation experiments with isolates AN 154 ( C. rosea ), AN 169 ( C. catenulatum ) and AN 171 ( T. aggressivum ). Experiments were carried out at 25°C, in liquid Czapek-Dox medium supplemented with yeast extract and zearalenone.

    Journal: BMC Microbiology

    Article Title: Zearalenone lactonohydrolase activity in Hypocreales and its evolutionary relationships within the epoxide hydrolase subset of a/b-hydrolases

    doi: 10.1186/1471-2180-14-82

    Figure Lengend Snippet: Relative normalized expression (N-fold) of zearalenone lactonohydrolase transcripts during incubation experiments with isolates AN 154 ( C. rosea ), AN 169 ( C. catenulatum ) and AN 171 ( T. aggressivum ). Experiments were carried out at 25°C, in liquid Czapek-Dox medium supplemented with yeast extract and zearalenone.

    Article Snippet: Sample preparation The fungal mycelium was grown in 50 ml Czapek-Dox broth (Sigma-Aldrich) with Yeast Extract (Oxoid) for 9 days at 25°C with rotary shaking at 100 rpm.

    Techniques: Expressing, Incubation

    UV–visible spectrum of a) Dox-NP™ liposomes in PBS, b) DoxHCl in PBS at the same (40 μg/mL) doxorubicin concentration.

    Journal: International Journal of Pharmaceutics

    Article Title: Analytical ultracentrifugation for analysis of doxorubicin loaded liposomes

    doi: 10.1016/j.ijpharm.2017.03.046

    Figure Lengend Snippet: UV–visible spectrum of a) Dox-NP™ liposomes in PBS, b) DoxHCl in PBS at the same (40 μg/mL) doxorubicin concentration.

    Article Snippet: 2.1 Materials Doxorubicin containing Dox-NP™ and control empty liposomes were purchased from Avanti PolarLipids.

    Techniques: Concentration Assay

    Photos of G2 in liquid media (top); from left to right: Czapek dox, 2% malt extract, potato dextrose, YPSS, YESD, and PYG broths. Photos of G2 on solid media (bottom); from left to right: rice, grits, oatmeal, wheat germ, and 3:1:1:1, respectively, of the same. Enlargements of the photos (2.5×) are provided in the lower right hand corner to help visualize the production of spores (orange) and/or exudates (black).

    Journal: AMB Express

    Article Title: Evaluation of culture media for the production of secondary metabolites in a natural products screening program

    doi: 10.1186/2191-0855-3-71

    Figure Lengend Snippet: Photos of G2 in liquid media (top); from left to right: Czapek dox, 2% malt extract, potato dextrose, YPSS, YESD, and PYG broths. Photos of G2 on solid media (bottom); from left to right: rice, grits, oatmeal, wheat germ, and 3:1:1:1, respectively, of the same. Enlargements of the photos (2.5×) are provided in the lower right hand corner to help visualize the production of spores (orange) and/or exudates (black).

    Article Snippet: These broths included Czapek Dox (CD; Sigma Aldrich), 2% Malt Extract (ME; Difco), Potato Dextrose (PD; Difco), YPSS (4 g yeast extract, 14 g soluble starch, 1 g dibasic K2 HPO4 , 0.5 g MgSO4 •7H2 O, 1 L H2 O), YESD (20 g soy peptone, 20 g dextrose, 5 g yeast extract, 1 L H2 O), and PYG (1.25 g soy peptone, 1.25 g yeast extract, 5 g D-glucose, 1 L H2 O).

    Techniques:

    Growth of Clonostachys rosea on milk powder plates to assess extracellular protease activity. Clonostachys rosea was inoculated to plates that contained ( a ) water agar, ( b ) potato dextrose agar, ( c ) malt extract agar and ( d ) Czapek dox agar, and the clearing zones (indicative of extracellular protease activity) were assessed both from above and below the agar plates seven days post inoculation

    Journal: BMC Evolutionary Biology

    Article Title: Comparative evolutionary histories of fungal proteases reveal gene gains in the mycoparasitic and nematode-parasitic fungus Clonostachys rosea

    doi: 10.1186/s12862-018-1291-1

    Figure Lengend Snippet: Growth of Clonostachys rosea on milk powder plates to assess extracellular protease activity. Clonostachys rosea was inoculated to plates that contained ( a ) water agar, ( b ) potato dextrose agar, ( c ) malt extract agar and ( d ) Czapek dox agar, and the clearing zones (indicative of extracellular protease activity) were assessed both from above and below the agar plates seven days post inoculation

    Article Snippet: Protease activity estimation using milk powder plate assay Extracellular protease activity of C. rosea was estimated using a milk powder plate assay in four different growth media i.e. potato dextrose agar (PDA, Sigma-Aldrich, Steinheim, Germany), malt extract agar (MEA, Sigma-Aldrich, Steinheim, Germany), Czapek Dox agar (CZ, Sigma-Aldrich, Steinheim, Germany) and water agar.

    Techniques: Activity Assay

    Active XBP1s is not sufficient to induce GRASP55 expression. ( a ) Timeline of experiment with overexpression of Dox-inducible active transcription factors ATF6 and XBP1s. ( b , c ) Lentiviral transduction was used to express active transcription factors XBP1s and ATF6 in primary cortical neurons. XBP1s and ATF6 should only be expressed upon Dox addition (TetON construct). Timeline as represented in ( a ). mRNA expression levels were analyzed by qPCR of UPR target genes ( b ), GRASP55 and GRASP65 ( c ) upon Dox addition (1 µg/ml). Data are shown as fold change difference over the expression of a control construct with a similar backbone (TetON) in the presence of Dox (baseline, set to 1). Significant differences of n independent experiments ( n is shown in bars) were tested via one-way ANOVA followed by post hoc Dunnett’s multiple comparison test compared to baseline.

    Journal: Scientific Reports

    Article Title: Unconventional secretion factor GRASP55 is increased by pharmacological unfolded protein response inducers in neurons

    doi: 10.1038/s41598-018-38146-6

    Figure Lengend Snippet: Active XBP1s is not sufficient to induce GRASP55 expression. ( a ) Timeline of experiment with overexpression of Dox-inducible active transcription factors ATF6 and XBP1s. ( b , c ) Lentiviral transduction was used to express active transcription factors XBP1s and ATF6 in primary cortical neurons. XBP1s and ATF6 should only be expressed upon Dox addition (TetON construct). Timeline as represented in ( a ). mRNA expression levels were analyzed by qPCR of UPR target genes ( b ), GRASP55 and GRASP65 ( c ) upon Dox addition (1 µg/ml). Data are shown as fold change difference over the expression of a control construct with a similar backbone (TetON) in the presence of Dox (baseline, set to 1). Significant differences of n independent experiments ( n is shown in bars) were tested via one-way ANOVA followed by post hoc Dunnett’s multiple comparison test compared to baseline.

    Article Snippet: Truncated ATF6, spliced XBP1 and control constructs were cloned into the vector pDESTSIN-TRE-Syn-rtTA2 (cloned from a construct received from Dr. P. E. Schätzle), producing a TetON lentiviral vector where expression is controlled by doxycycline (Dox) (Sigma-Aldrich). pLKO.1 plasmids encoding short hairpin (sh)RNAs against mouse GRASP55 (Table ) and the scrambled control (scRNA) plasmid were obtained from the MISSION shRNA library (Sigma-Aldrich).

    Techniques: Expressing, Over Expression, Transduction, Construct, Real-time Polymerase Chain Reaction

    Experimental design. ( A ) A schematic of the HIV-1 and HIV-rtTA genome is shown, with the LTR subdivided in U3, R and U5 domains. In HIV-rtTA, the Tat-TAR axis of transcription regulation was inactivated by multiple nucleotide substitutions in the bulge and loop sequences of TAR (TAR mut , crossed boxes). Transcription and replication of the virus were made dox dependent by the introduction of tetO elements in the U3 region of the LTR promoter and replacement of the nef gene by the rtTA gene. The short TAR-only and full-length RNA molecules produced by non-processive (in the absence of Tat [-Tat] or dox [-dox]) and processive transcription (in the presence of Tat [+Tat] or dox [+dox]), respectively, are shown. ( B ) Structure of the TAR hairpin of HIV-1 (TAR wt ) and HIV-rtTA (TAR mut ). The bulge and loop mutations present in TAR mut are boxed in black. ( C ) Strategy used to identify AGO2-bound viral RNAs.

    Journal: Nucleic Acids Research

    Article Title: Tat-dependent production of an HIV-1 TAR-encoded miRNA-like small RNA

    doi: 10.1093/nar/gkw167

    Figure Lengend Snippet: Experimental design. ( A ) A schematic of the HIV-1 and HIV-rtTA genome is shown, with the LTR subdivided in U3, R and U5 domains. In HIV-rtTA, the Tat-TAR axis of transcription regulation was inactivated by multiple nucleotide substitutions in the bulge and loop sequences of TAR (TAR mut , crossed boxes). Transcription and replication of the virus were made dox dependent by the introduction of tetO elements in the U3 region of the LTR promoter and replacement of the nef gene by the rtTA gene. The short TAR-only and full-length RNA molecules produced by non-processive (in the absence of Tat [-Tat] or dox [-dox]) and processive transcription (in the presence of Tat [+Tat] or dox [+dox]), respectively, are shown. ( B ) Structure of the TAR hairpin of HIV-1 (TAR wt ) and HIV-rtTA (TAR mut ). The bulge and loop mutations present in TAR mut are boxed in black. ( C ) Strategy used to identify AGO2-bound viral RNAs.

    Article Snippet: Small RNA library preparation and SOLiD deep sequencing 293T cells (5 × 106 cells per 25 cm2 flask) were co-transfected with 5 μg AGO2-FLAG plasmid ( ) and 20 μg HIV-1 , HIV-rtTA-Tatwt ( ) or pBluescript plasmid (Stratagene), and cultured for 48 h. HIV-rtTA transfected cells were cultured with 1 μg/ml dox (Sigma; D-9891).

    Techniques: Produced

    Tat activates miR-TAR-3p production. ( A ) 293T cells were transfected with the HIV-1 plasmid or with the Tat-deficient HIV-Tat stop construct and a varying amount of Tat plasmid (0, 50 or 500 ng). The CA-p24 level in the culture supernatant was measured after 48 h. The mean ±SD for three experiments is shown. ( B ) Small RNAs were isolated and analysed by northern blotting using the miR-TAR-3p probe, as described for Figure 3A . ( C ) 293T cells were transfected with the Tat-deficient HIV-rtTA-Tat stop construct with a wild-type TAR element (TAR wt ). When indicated, cells were co-transfected with 500 ng pTat and cultured in the presence of dox. The CA-p24 level in the culture supernatant was measured after 48 h. The mean ±SD for three experiments is shown. ( D ) Small RNAs isolated from the transfected cells were analysed by northern blotting using the miR-TAR-3p probe, as described for Figure 3A .

    Journal: Nucleic Acids Research

    Article Title: Tat-dependent production of an HIV-1 TAR-encoded miRNA-like small RNA

    doi: 10.1093/nar/gkw167

    Figure Lengend Snippet: Tat activates miR-TAR-3p production. ( A ) 293T cells were transfected with the HIV-1 plasmid or with the Tat-deficient HIV-Tat stop construct and a varying amount of Tat plasmid (0, 50 or 500 ng). The CA-p24 level in the culture supernatant was measured after 48 h. The mean ±SD for three experiments is shown. ( B ) Small RNAs were isolated and analysed by northern blotting using the miR-TAR-3p probe, as described for Figure 3A . ( C ) 293T cells were transfected with the Tat-deficient HIV-rtTA-Tat stop construct with a wild-type TAR element (TAR wt ). When indicated, cells were co-transfected with 500 ng pTat and cultured in the presence of dox. The CA-p24 level in the culture supernatant was measured after 48 h. The mean ±SD for three experiments is shown. ( D ) Small RNAs isolated from the transfected cells were analysed by northern blotting using the miR-TAR-3p probe, as described for Figure 3A .

    Article Snippet: Small RNA library preparation and SOLiD deep sequencing 293T cells (5 × 106 cells per 25 cm2 flask) were co-transfected with 5 μg AGO2-FLAG plasmid ( ) and 20 μg HIV-1 , HIV-rtTA-Tatwt ( ) or pBluescript plasmid (Stratagene), and cultured for 48 h. HIV-rtTA transfected cells were cultured with 1 μg/ml dox (Sigma; D-9891).

    Techniques: Transfection, Plasmid Preparation, Construct, Isolation, Northern Blot, Cell Culture

    TAR and miR-TAR-3p RNAs are produced by non-processive transcription. ( A–B ) 293T cells were transfected with the HIV-1 and HIV-rtTA constructs and cultured for 48 h without dox (HIV-1 and HIV-rtTA) or with dox (HIV-rtTA). ( A ) Intracellular small RNAs were isolated and analysed by northern blotting. An LNA probe complementary to the miR-TAR-3p sequence was used to identify the position of miR-TAR-3p and TAR RNAs. The position of the size markers is shown on the left. Ethidium bromide staining of small 5S rRNA (5S) and tRNAs is shown as loading control below the blot. ( B ) The CA-p24 level in the culture supernatant was measured to quantify gene expression. The mean ±SD for three experiments is shown. ( C ) Expected TAR-containing transcripts produced by the HIV-rtTA variants with the TAR mut element in both the 5′ and 3′LTR, only the 5′ or 3′ LTR, or no TAR element (Δ, TAR deletion). In the 3′TAR+3′mutSp1 variant, the TAR element in the 5′ LTR was deleted and the Sp1 binding sequences in the 3′ LTR region were mutated. This set of variants is based on the HIV-rtTA-Tat Y26A variant ( 47 ) and all constructs carry the Tat Y26A mutation (in addition to the TAR mut mutations) to disable the Tat-TAR transcription activation mechanism.( D ) 293T cells were transfected with the HIV-rtTA constructs shown in panel C and cultured for 48 h with dox. Small RNAs were isolated and analysed by northern blotting using the miR-TAR-3p probe, as described for panel A. ( E ) CA-p24 production was analysed as described for panel B. The mean ±SD for three experiments is shown. ( F ) CD4-enriched human PBMC were infected with 293T-produced wt HIV-1 virus and cultured for 48 h. Small RNAs were isolated and northern blot analysis was performed as described for Figure 3A . Mock, control uninfected PBMC. The RNA size marker is shown at the right (a short phosphorimager exposure is shown for this lane because of the high radio activity of the 32 P-labeled marker fragments).

    Journal: Nucleic Acids Research

    Article Title: Tat-dependent production of an HIV-1 TAR-encoded miRNA-like small RNA

    doi: 10.1093/nar/gkw167

    Figure Lengend Snippet: TAR and miR-TAR-3p RNAs are produced by non-processive transcription. ( A–B ) 293T cells were transfected with the HIV-1 and HIV-rtTA constructs and cultured for 48 h without dox (HIV-1 and HIV-rtTA) or with dox (HIV-rtTA). ( A ) Intracellular small RNAs were isolated and analysed by northern blotting. An LNA probe complementary to the miR-TAR-3p sequence was used to identify the position of miR-TAR-3p and TAR RNAs. The position of the size markers is shown on the left. Ethidium bromide staining of small 5S rRNA (5S) and tRNAs is shown as loading control below the blot. ( B ) The CA-p24 level in the culture supernatant was measured to quantify gene expression. The mean ±SD for three experiments is shown. ( C ) Expected TAR-containing transcripts produced by the HIV-rtTA variants with the TAR mut element in both the 5′ and 3′LTR, only the 5′ or 3′ LTR, or no TAR element (Δ, TAR deletion). In the 3′TAR+3′mutSp1 variant, the TAR element in the 5′ LTR was deleted and the Sp1 binding sequences in the 3′ LTR region were mutated. This set of variants is based on the HIV-rtTA-Tat Y26A variant ( 47 ) and all constructs carry the Tat Y26A mutation (in addition to the TAR mut mutations) to disable the Tat-TAR transcription activation mechanism.( D ) 293T cells were transfected with the HIV-rtTA constructs shown in panel C and cultured for 48 h with dox. Small RNAs were isolated and analysed by northern blotting using the miR-TAR-3p probe, as described for panel A. ( E ) CA-p24 production was analysed as described for panel B. The mean ±SD for three experiments is shown. ( F ) CD4-enriched human PBMC were infected with 293T-produced wt HIV-1 virus and cultured for 48 h. Small RNAs were isolated and northern blot analysis was performed as described for Figure 3A . Mock, control uninfected PBMC. The RNA size marker is shown at the right (a short phosphorimager exposure is shown for this lane because of the high radio activity of the 32 P-labeled marker fragments).

    Article Snippet: Small RNA library preparation and SOLiD deep sequencing 293T cells (5 × 106 cells per 25 cm2 flask) were co-transfected with 5 μg AGO2-FLAG plasmid ( ) and 20 μg HIV-1 , HIV-rtTA-Tatwt ( ) or pBluescript plasmid (Stratagene), and cultured for 48 h. HIV-rtTA transfected cells were cultured with 1 μg/ml dox (Sigma; D-9891).

    Techniques: Produced, Transfection, Construct, Cell Culture, Isolation, Northern Blot, Sequencing, Staining, Expressing, Variant Assay, Binding Assay, Mutagenesis, Activation Assay, Infection, Marker, Activity Assay, Labeling

    Transgene induction 4 weeks after transverse aortic constriction (TAC). (A) GLUT1 protein content in whole heart homogenates 2 days after DOX injection (mice were 10 weeks at the time of DOX injections). (B) Glucose uptake in isolated cardiomyocytes from Cont and G1HA hearts 2 days after DOX injection. Data are expressed as means±SEM. Significant differences were determined by Student's t ‐test. (*) vs Cont. Two‐way ANOVA was performed to analyze differences by treatment and genotype, including a Tukey post hoc analysis when significant interaction occurred. (C) Representative GLUT1 and GLUT4 immunoblot in Cont and G1HA hearts 4 weeks after TAC. (D) Densitometry of GLUT1 protein ( P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Inducible Overexpression of GLUT1 Prevents Mitochondrial Dysfunction and Attenuates Structural Remodeling in Pressure Overload but Does Not Prevent Left Ventricular Dysfunction

    doi: 10.1161/JAHA.113.000301

    Figure Lengend Snippet: Transgene induction 4 weeks after transverse aortic constriction (TAC). (A) GLUT1 protein content in whole heart homogenates 2 days after DOX injection (mice were 10 weeks at the time of DOX injections). (B) Glucose uptake in isolated cardiomyocytes from Cont and G1HA hearts 2 days after DOX injection. Data are expressed as means±SEM. Significant differences were determined by Student's t ‐test. (*) vs Cont. Two‐way ANOVA was performed to analyze differences by treatment and genotype, including a Tukey post hoc analysis when significant interaction occurred. (C) Representative GLUT1 and GLUT4 immunoblot in Cont and G1HA hearts 4 weeks after TAC. (D) Densitometry of GLUT1 protein ( P

    Article Snippet: Both Cont and G1HA mice were injected with DOX and then kept on DOX‐chow for 4 weeks.

    Techniques: Injection, Mouse Assay, Isolation

    Ectopic PAR-1 activity restores KPC2 Par-1 KO cell tumor growth. A, Anti-Myc immunofluorescence of KPC2 Par-1 KO/Tg cells treated ± Dox. B, qRT-PCR for endogenous and Tg Par-1 transcripts comparing KPC2 WT and KPC2 Par-1 KO/Tg cells ± Dox stimulation (N = 3 per group). C, Immunoblot for phospho-ERK in KPC2 cells containing a Tet-inducible luciferase expression vector (KPC2 TetLuc ) and KPC2 Par-1 KO/Tg cells stimulated ± Dox and ± thrombin. D and E, Analysis of tumor volume and final tumor mass following subcutaneous injection of KPC2 WT cells or KPC2 Par-1 KO1 cells ± Dox ( N = 8 mice per group). F and G, Analysis of tumor volume and final tumor mass following subcutaneous injection of KPC2 Par-1 KO/Tg cells into cohorts of mice treated ± Dox ( N = 12 mice per group). Scale bars, 25 μm. **, P

    Journal: Cancer research

    Article Title: Thrombin Signaling Promotes Pancreatic Adenocarcinoma through PAR-1–Dependent Immune Evasion

    doi: 10.1158/0008-5472.CAN-18-3206

    Figure Lengend Snippet: Ectopic PAR-1 activity restores KPC2 Par-1 KO cell tumor growth. A, Anti-Myc immunofluorescence of KPC2 Par-1 KO/Tg cells treated ± Dox. B, qRT-PCR for endogenous and Tg Par-1 transcripts comparing KPC2 WT and KPC2 Par-1 KO/Tg cells ± Dox stimulation (N = 3 per group). C, Immunoblot for phospho-ERK in KPC2 cells containing a Tet-inducible luciferase expression vector (KPC2 TetLuc ) and KPC2 Par-1 KO/Tg cells stimulated ± Dox and ± thrombin. D and E, Analysis of tumor volume and final tumor mass following subcutaneous injection of KPC2 WT cells or KPC2 Par-1 KO1 cells ± Dox ( N = 8 mice per group). F and G, Analysis of tumor volume and final tumor mass following subcutaneous injection of KPC2 Par-1 KO/Tg cells into cohorts of mice treated ± Dox ( N = 12 mice per group). Scale bars, 25 μm. **, P

    Article Snippet: In the experiments of activating Tet-PAR-1 expression in vivo , mice were provided Dox chow (ENVIGO, TF 08541) with food replaced every 6 days.

    Techniques: Activity Assay, Immunofluorescence, Quantitative RT-PCR, Luciferase, Expressing, Plasmid Preparation, Injection, Mouse Assay

    Expression of PAR-1 drives tumor growth in the pancreas microenvironment, resulting in poor host survival. A, KPC2 WT or KPC2 Par-1 KO/Tg cells were injected into the pancreas and mice were treated ± Dox ( N = 12 mice per group). Pancreas tumors were harvested at 21 days postsurgery. B, Representative examples of isolated tumors from the pancreas of mice 21 days postsurgery. Asterisk indicates one of only two tumors generated out of a cohort of 12 mice from the Par-1 KO/Tg cells in the absence of Dox. The remaining 10 animals failed to develop visible tumors within the 21-day period. C, Survival study following orthotopic pancreas injection of KPC2 WT vs. KPC2 Par-1 KO1 cells ( N = 12 mice per group). Median survival times are indicated for each study (red dot). Scale bar, 2 mm. ****, P

    Journal: Cancer research

    Article Title: Thrombin Signaling Promotes Pancreatic Adenocarcinoma through PAR-1–Dependent Immune Evasion

    doi: 10.1158/0008-5472.CAN-18-3206

    Figure Lengend Snippet: Expression of PAR-1 drives tumor growth in the pancreas microenvironment, resulting in poor host survival. A, KPC2 WT or KPC2 Par-1 KO/Tg cells were injected into the pancreas and mice were treated ± Dox ( N = 12 mice per group). Pancreas tumors were harvested at 21 days postsurgery. B, Representative examples of isolated tumors from the pancreas of mice 21 days postsurgery. Asterisk indicates one of only two tumors generated out of a cohort of 12 mice from the Par-1 KO/Tg cells in the absence of Dox. The remaining 10 animals failed to develop visible tumors within the 21-day period. C, Survival study following orthotopic pancreas injection of KPC2 WT vs. KPC2 Par-1 KO1 cells ( N = 12 mice per group). Median survival times are indicated for each study (red dot). Scale bar, 2 mm. ****, P

    Article Snippet: In the experiments of activating Tet-PAR-1 expression in vivo , mice were provided Dox chow (ENVIGO, TF 08541) with food replaced every 6 days.

    Techniques: Expressing, Injection, Mouse Assay, Isolation, Generated

    Schematic illustration of the stealth property and promoted tumor cell uptake of nanoconjugates ( A ) and DOX-loaded nanoconjugates (DOX/HDPEPM) ( B ). Notes: Depicted in ( A ), nanoconjugates minimize nonspecific interactions with serum components and change the surface charge of nanoconjugates in response to the tumor acidity (pH e ), leading to promoted cell internalization by the combination of electrostatic absorptive endocytosis and receptor-mediated endocytosis. Abbreviations: DMA, 2,3-dimethylmaleic anhydride; DOX, doxorubicin; HDPEPM, nanoconjugate formed by covalent attachment of fragment HAb18 F(ab′) 2 and 2,3-dimethylmaleic anhydride to polyethylenimine-modified poly(β-L-malic acid); PEI, polyethylenimine; PMLA, poly(β-L-malic acid).

    Journal: International Journal of Nanomedicine

    Article Title: Preparation of poly(β-L-malic acid)-based charge-conversional nanoconjugates for tumor-specific uptake and cellular delivery

    doi: 10.2147/IJN.S78547

    Figure Lengend Snippet: Schematic illustration of the stealth property and promoted tumor cell uptake of nanoconjugates ( A ) and DOX-loaded nanoconjugates (DOX/HDPEPM) ( B ). Notes: Depicted in ( A ), nanoconjugates minimize nonspecific interactions with serum components and change the surface charge of nanoconjugates in response to the tumor acidity (pH e ), leading to promoted cell internalization by the combination of electrostatic absorptive endocytosis and receptor-mediated endocytosis. Abbreviations: DMA, 2,3-dimethylmaleic anhydride; DOX, doxorubicin; HDPEPM, nanoconjugate formed by covalent attachment of fragment HAb18 F(ab′) 2 and 2,3-dimethylmaleic anhydride to polyethylenimine-modified poly(β-L-malic acid); PEI, polyethylenimine; PMLA, poly(β-L-malic acid).

    Article Snippet: Materials PEI with a molecular weight of 1.8 kDa, branched, and DOX hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Techniques: Modification

    Synthesis of DOX-loaded, PEI, fragment HAb18 F(ab′) 2 and DMA decorated PMLA (DOX/HDPEPM) nanoconjugates. Abbreviations: DMA, 2,3-dimethylmaleic anhydride; DOX, doxorubicin; EDC, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide; HDPEPM, nanoconjugate formed by covalent attachment of fragment HAb18 F(ab′) 2 and 2,3-dimethylmaleic anhydride to polyethylenimine-modified poly(β-L-malic acid); Mal, maleimide; NHS, N -hydroxysuccinimide; PEG, polyethylene glycol; PEI, polyethylenimine; PMLA, poly(β-L-malic acid).

    Journal: International Journal of Nanomedicine

    Article Title: Preparation of poly(β-L-malic acid)-based charge-conversional nanoconjugates for tumor-specific uptake and cellular delivery

    doi: 10.2147/IJN.S78547

    Figure Lengend Snippet: Synthesis of DOX-loaded, PEI, fragment HAb18 F(ab′) 2 and DMA decorated PMLA (DOX/HDPEPM) nanoconjugates. Abbreviations: DMA, 2,3-dimethylmaleic anhydride; DOX, doxorubicin; EDC, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide; HDPEPM, nanoconjugate formed by covalent attachment of fragment HAb18 F(ab′) 2 and 2,3-dimethylmaleic anhydride to polyethylenimine-modified poly(β-L-malic acid); Mal, maleimide; NHS, N -hydroxysuccinimide; PEG, polyethylene glycol; PEI, polyethylenimine; PMLA, poly(β-L-malic acid).

    Article Snippet: Materials PEI with a molecular weight of 1.8 kDa, branched, and DOX hydrochloride were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Techniques: Modification

    Curcumin downregulates P-gp and S100A8 protein expression in K562/DOX cells. (A) K562/DOX cells were treated with curcumin (0.5, 1 or 2 µM) for 48 h, and western blot analysis was performed to detect the protein expression levels of (B) P-gp and (C) S100A8. (D) K562/DOX cells were treated with curcumin (2 µM) for 24, 48 or 72 h, followed by western blot analysis of (E) P-gp and (F) S100A8 protein expression. **P

    Journal: Oncology Letters

    Article Title: Curcumin increases the sensitivity of K562/DOX cells to doxorubicin by targeting S100 calcium-binding protein A8 and P-glycoprotein

    doi: 10.3892/ol.2019.11083

    Figure Lengend Snippet: Curcumin downregulates P-gp and S100A8 protein expression in K562/DOX cells. (A) K562/DOX cells were treated with curcumin (0.5, 1 or 2 µM) for 48 h, and western blot analysis was performed to detect the protein expression levels of (B) P-gp and (C) S100A8. (D) K562/DOX cells were treated with curcumin (2 µM) for 24, 48 or 72 h, followed by western blot analysis of (E) P-gp and (F) S100A8 protein expression. **P

    Article Snippet: Cell culture The human CML cell lines K562 and K562/DOX were obtained from Nanjing KeyGen Biotech Co., Ltd.

    Techniques: Expressing, Western Blot

    Curcumin increases intracellular accumulation of RHO-123 and DOX in K562/DOX cells. (A) Effects of VRP (10 µM) and curcumin (0.5, 1 or 2 µM) on intracellular accumulation of RHO-123 was detected by flow cytometry. ‘FL-1H’ means RHO-123 emitting light detection channel, x-axis means fluorescence signal was detected by FL-1H. (B) A quantification of the effect of VRP (10 µM) and curcumin (0.5, 1 or 2 µM) on intracellular accumulation of RHO-123. (C) Effects of VRP (10 Μm) and curcumin (0.5, 1 or 2 Μm) on intracellular accumulation of DOX. **P

    Journal: Oncology Letters

    Article Title: Curcumin increases the sensitivity of K562/DOX cells to doxorubicin by targeting S100 calcium-binding protein A8 and P-glycoprotein

    doi: 10.3892/ol.2019.11083

    Figure Lengend Snippet: Curcumin increases intracellular accumulation of RHO-123 and DOX in K562/DOX cells. (A) Effects of VRP (10 µM) and curcumin (0.5, 1 or 2 µM) on intracellular accumulation of RHO-123 was detected by flow cytometry. ‘FL-1H’ means RHO-123 emitting light detection channel, x-axis means fluorescence signal was detected by FL-1H. (B) A quantification of the effect of VRP (10 µM) and curcumin (0.5, 1 or 2 µM) on intracellular accumulation of RHO-123. (C) Effects of VRP (10 Μm) and curcumin (0.5, 1 or 2 Μm) on intracellular accumulation of DOX. **P

    Article Snippet: Cell culture The human CML cell lines K562 and K562/DOX were obtained from Nanjing KeyGen Biotech Co., Ltd.

    Techniques: Flow Cytometry, Cytometry, Fluorescence

    Silencing of S100A8 increases the sensitivity of K562/DOX cells to DOX, however S100A8 does not affect the expression of P-gp or vice versa . (A) Effects of silencing of S100A8 or P-gp on the protein expression of S100A8 and P-gp. (B) Effects of DOX on the viability of K562/DOX cells after silencing of S100A8. (C) Effects of S100A8-silencing on intracellular accumulation of DOX. ***P

    Journal: Oncology Letters

    Article Title: Curcumin increases the sensitivity of K562/DOX cells to doxorubicin by targeting S100 calcium-binding protein A8 and P-glycoprotein

    doi: 10.3892/ol.2019.11083

    Figure Lengend Snippet: Silencing of S100A8 increases the sensitivity of K562/DOX cells to DOX, however S100A8 does not affect the expression of P-gp or vice versa . (A) Effects of silencing of S100A8 or P-gp on the protein expression of S100A8 and P-gp. (B) Effects of DOX on the viability of K562/DOX cells after silencing of S100A8. (C) Effects of S100A8-silencing on intracellular accumulation of DOX. ***P

    Article Snippet: Cell culture The human CML cell lines K562 and K562/DOX were obtained from Nanjing KeyGen Biotech Co., Ltd.

    Techniques: Expressing

    Curcumin enhances the cytotoxicity of in K562/DOX cells. (A) Chemical structure of curcumin. K562 and K562/DOX cells were treated with (B) DOX (0–50 µM) and (C) with curcumin (0–32 µM) for 48 h. (D) K562/DOX and (E) K562 cells were pre-treated with curcumin (0.5, 1 and 2 µM) for 24 h, followed by incubation with various concentrations of DOX for an additional 48 h. ***P

    Journal: Oncology Letters

    Article Title: Curcumin increases the sensitivity of K562/DOX cells to doxorubicin by targeting S100 calcium-binding protein A8 and P-glycoprotein

    doi: 10.3892/ol.2019.11083

    Figure Lengend Snippet: Curcumin enhances the cytotoxicity of in K562/DOX cells. (A) Chemical structure of curcumin. K562 and K562/DOX cells were treated with (B) DOX (0–50 µM) and (C) with curcumin (0–32 µM) for 48 h. (D) K562/DOX and (E) K562 cells were pre-treated with curcumin (0.5, 1 and 2 µM) for 24 h, followed by incubation with various concentrations of DOX for an additional 48 h. ***P

    Article Snippet: Cell culture The human CML cell lines K562 and K562/DOX were obtained from Nanjing KeyGen Biotech Co., Ltd.

    Techniques: Incubation

    Addition of curcumin enhances apoptosis induced by DOX in K562/DOX cells. (A) Effects of curcumin (2 µM), DOX (4 µM) and si-S100A8 used alone or in combination on the intracellular free calcium ion concentration in K562/DOX cells. Effects of curcumin (2 µM), DOX (4 µM) and si-S100A8 used alone or in combination on the protein expression of (B) Bcl-xL, Bax and (C) CHOP in K562/DOX cells. (D) Effects of curcumin (2 µM), DOX (4 µM) and si-S100A8 used alone or in combination on caspase-3 activity. (E) K562/DOX cells were stained with DAPI after pre-treatment and observed under an inverted fluorescence microscope (magnification, ×40) and the relative fluorescence intensity analysis of DAPI was determined. DNA fragmentation is indicated by red arrows. ***P

    Journal: Oncology Letters

    Article Title: Curcumin increases the sensitivity of K562/DOX cells to doxorubicin by targeting S100 calcium-binding protein A8 and P-glycoprotein

    doi: 10.3892/ol.2019.11083

    Figure Lengend Snippet: Addition of curcumin enhances apoptosis induced by DOX in K562/DOX cells. (A) Effects of curcumin (2 µM), DOX (4 µM) and si-S100A8 used alone or in combination on the intracellular free calcium ion concentration in K562/DOX cells. Effects of curcumin (2 µM), DOX (4 µM) and si-S100A8 used alone or in combination on the protein expression of (B) Bcl-xL, Bax and (C) CHOP in K562/DOX cells. (D) Effects of curcumin (2 µM), DOX (4 µM) and si-S100A8 used alone or in combination on caspase-3 activity. (E) K562/DOX cells were stained with DAPI after pre-treatment and observed under an inverted fluorescence microscope (magnification, ×40) and the relative fluorescence intensity analysis of DAPI was determined. DNA fragmentation is indicated by red arrows. ***P

    Article Snippet: Cell culture The human CML cell lines K562 and K562/DOX were obtained from Nanjing KeyGen Biotech Co., Ltd.

    Techniques: Concentration Assay, Expressing, Activity Assay, Staining, Fluorescence, Microscopy

    VEGF + fat transplants improve glucose tolerance upon an HFD challenge. A : Schematic diagram of sWAT transplantation. Wild-type controls and VEGF-Tg mice were fed with a chow diet containing dox (60 mg/ kg) for 1 week. sWAT from either control mice or mice expressing VEGF + was harvested and subsequently implanted into C57/BL6J wild-type hosts. The recipient mice were fed with an HFD containing a low dose of dox (60 mg/ kg) for 3 weeks. B : Glucose levels were determined during an OGTT 3 weeks posttransplantion after continued HFD feeding. The difference at each time point was analyzed by Student t test. * P

    Journal: Diabetes

    Article Title: VEGF-A–Expressing Adipose Tissue Shows Rapid Beiging and Enhanced Survival After Transplantation and Confers IL-4–Independent Metabolic Improvements

    doi: 10.2337/db16-1081

    Figure Lengend Snippet: VEGF + fat transplants improve glucose tolerance upon an HFD challenge. A : Schematic diagram of sWAT transplantation. Wild-type controls and VEGF-Tg mice were fed with a chow diet containing dox (60 mg/ kg) for 1 week. sWAT from either control mice or mice expressing VEGF + was harvested and subsequently implanted into C57/BL6J wild-type hosts. The recipient mice were fed with an HFD containing a low dose of dox (60 mg/ kg) for 3 weeks. B : Glucose levels were determined during an OGTT 3 weeks posttransplantion after continued HFD feeding. The difference at each time point was analyzed by Student t test. * P

    Article Snippet: For low dosage of dox treatment in combination with HFD experiments, all mice (including controls) were fed with the HFD paste (Research Diets, Inc.) mixed with dox powder (Sigma-Aldrich) to a final concentration of 60 mg/kg.

    Techniques: Transplantation Assay, Mouse Assay, Expressing

    The survival rate of adipocytes in VEGF + fat grafts is increased compared with wild-type fat transplants. Control and VEGF-A + fat implants were taken from the host 3 weeks after the HFD challenge containing a low dose of dox (60 mg/kg). sWAT fat grafts from both groups (wild type, left panels; Tg, right panels) were subjected to histological analysis ( n = 5/group). Two representative fields are shown for each condition. A : H E stain. Scale bars = 50 μm. B : Antiendomucin stain. Scale bars = 50 μm. C : Anti-UCP1 stain. Scale bars = 200 μm. D : Immunofluorescence stain for MAC-2 (macrophage marker, green) and perilipin (live adipocyte marker, red). Costain with DAPI (nuclei, blue). Scale bars = 100 μm. Wild-type controls in top panel; Tg in the bottom panel. E : The ratio of perilipin + and MAC-2 + cell populations was calculated.

    Journal: Diabetes

    Article Title: VEGF-A–Expressing Adipose Tissue Shows Rapid Beiging and Enhanced Survival After Transplantation and Confers IL-4–Independent Metabolic Improvements

    doi: 10.2337/db16-1081

    Figure Lengend Snippet: The survival rate of adipocytes in VEGF + fat grafts is increased compared with wild-type fat transplants. Control and VEGF-A + fat implants were taken from the host 3 weeks after the HFD challenge containing a low dose of dox (60 mg/kg). sWAT fat grafts from both groups (wild type, left panels; Tg, right panels) were subjected to histological analysis ( n = 5/group). Two representative fields are shown for each condition. A : H E stain. Scale bars = 50 μm. B : Antiendomucin stain. Scale bars = 50 μm. C : Anti-UCP1 stain. Scale bars = 200 μm. D : Immunofluorescence stain for MAC-2 (macrophage marker, green) and perilipin (live adipocyte marker, red). Costain with DAPI (nuclei, blue). Scale bars = 100 μm. Wild-type controls in top panel; Tg in the bottom panel. E : The ratio of perilipin + and MAC-2 + cell populations was calculated.

    Article Snippet: For low dosage of dox treatment in combination with HFD experiments, all mice (including controls) were fed with the HFD paste (Research Diets, Inc.) mixed with dox powder (Sigma-Aldrich) to a final concentration of 60 mg/kg.

    Techniques: Staining, Immunofluorescence, Marker