Journal: Diagnostic Microbiology and Infectious Disease

Article Title: Reverse-transcription polymerase chain reaction/pyrosequencing to characterize neuraminidase H275 residue of influenza A 2009 H1N1 virus for rapid and specific detection of the viral oseltamivir resistance marker in a clinical laboratory

doi: 10.1016/j.diagmicrobio.2011.09.003

Figure Lengend Snippet: Pyrogram sequence results at residue H275 (highlighted) representing either oseltamivir-susceptible codon (CAC, underlined) (A) or oseltamivir-resistant codon (TAC, underlined) (B). The letters listed below the peaks of each panel are the nucleotides dispensed during pyrosequencing on the PyroMark ID system.

Article Snippet: Two pairs of primers located about 200 bp upstream and downstream from the H275 residue were developed by SeqWright (Houston, TX, USA) for both RT-PCR and Sanger sequencing.

Techniques: Sequencing, Residue

Journal: Diagnostic Microbiology and Infectious Disease

Article Title: Reverse-transcription polymerase chain reaction/pyrosequencing to characterize neuraminidase H275 residue of influenza A 2009 H1N1 virus for rapid and specific detection of the viral oseltamivir resistance marker in a clinical laboratory

doi: 10.1016/j.diagmicrobio.2011.09.003

Figure Lengend Snippet: Alignment of representative sequences by Sanger method (as “S”) and sequences by RT-PCR-pyroseq method (as “P”) to the reference sequence (CA04, GenBank number: 22780 9833: 620-1058, as described in ). Two clustered mutation residues, N248 (A248) and H275, are underlined and highlighted. Of the 16 nonclustered mutations detected in the Sanger sequencing, 2 nearest to the H275 residue from either direction are shown here (highlighted only).

Article Snippet: Two pairs of primers located about 200 bp upstream and downstream from the H275 residue were developed by SeqWright (Houston, TX, USA) for both RT-PCR and Sanger sequencing.

Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Mutagenesis, Residue

Journal: Diagnostic Microbiology and Infectious Disease

Article Title: Reverse-transcription polymerase chain reaction/pyrosequencing to characterize neuraminidase H275 residue of influenza A 2009 H1N1 virus for rapid and specific detection of the viral oseltamivir resistance marker in a clinical laboratory

doi: 10.1016/j.diagmicrobio.2011.09.003

Figure Lengend Snippet: Sensitivity testing of the RT-PCR-pyroseq method on 10-fold dilutions using real-time PCR Ct values in pandemic 2009 H1N1 virus identification as references

Article Snippet: Two pairs of primers located about 200 bp upstream and downstream from the H275 residue were developed by SeqWright (Houston, TX, USA) for both RT-PCR and Sanger sequencing.

Techniques: Real-time Polymerase Chain Reaction, Virus, Sequencing

Journal: Diagnostic Microbiology and Infectious Disease

Article Title: Reverse-transcription polymerase chain reaction/pyrosequencing to characterize neuraminidase H275 residue of influenza A 2009 H1N1 virus for rapid and specific detection of the viral oseltamivir resistance marker in a clinical laboratory

doi: 10.1016/j.diagmicrobio.2011.09.003

Figure Lengend Snippet: Specificity of the RT-PCR-pyroseq method for detection of 2009 H1N1 neuraminidase residue H275 sequences a

Article Snippet: Two pairs of primers located about 200 bp upstream and downstream from the H275 residue were developed by SeqWright (Houston, TX, USA) for both RT-PCR and Sanger sequencing.

Techniques: Residue, Amplification, Sequencing, Virus, Negative Control