Journal: Journal of Nuclear Medicine

Article Title: Advancing Targeted Radionuclide Therapy Through the National Cancer Institute’s Small Business Innovation Research Pathway

doi: 10.2967/jnumed.118.214684

Figure Lengend Snippet: NCI SBIR–Funded TRT Contracts from 2016 to 2017

Article Snippet: 2017 , Molecular Targeting Technologies, Inc. , Phase I , Glioblastoma , Radioiodine , SapC-DOPS nanovesicles , Create novel cancer-selective, targeted, radiolabeled SapC-DOPS for treatment of glioblastoma.

Techniques: Radioactivity, Drug discovery, Recombinant, Biomarker Discovery, In Vivo, Fluorescence, Binding Assay, Labeling, In Vitro, Produced, Software

Journal: Molecular Cell

Article Title: Osh Proteins Control Nanoscale Lipid Organization Necessary for PI(4,5)P 2 Synthesis

doi: 10.1016/j.molcel.2019.06.037

Figure Lengend Snippet: PIP5K Stimulation by Unsaturated PS and Sterols In Vitro (A) PI4P concentration dependence of zPIP5K activity in the absence and presence of 20 mol% cholesterol (Chol). Initial velocities (Vi) were determined from PIP5K reaction progress curves from real-time assays. Data represent mean ± SEM (n = 3). (B) The activity of zPIP5K toward 1 mol% bPI4P liposomes either lacking or containing bPS and/or cholesterol as indicated. Data represent mean values (n = 3; SEM < 0.25 × 10 3 ). (C) The activity of zPIP5K toward 1 mol% bPI4P liposomes containing the indicated PS species either lacking or containing cholesterol as indicated. Data represent mean values (n ≥ 6; SEM < 0.48 × 10 3 ). (D) PIP5K sedimentation assays using liposomes containing bPI4P, DOPS, and cholesterol as indicated. Data represent mean ± SEM (n = 3). (E) The activity of zPIP5K toward 1 mol% PO-PI4P liposomes containing the indicated PS species either lacking or containing ergosterol (Erg) as indicated. Data represent mean values (n = 3; SEM < 1.20 × 10 3 ). (F) PIP5K sedimentation assays using 1 mol% PO-PI4P liposomes either lacking or containing the indicated PS and/or Erg. Data represent mean ± SEM (n = 3). (G) Phospholipids used in the PIP5K assays. ∗ p < 0.05. See also Figure S3 .

Article Snippet: Time-averaged positions of the phosphate moieties of the phospholipids POPC (D), DOPS (E), and PI4P (G) or oxygen atoms in cholesterol (Chol, F) from all-atom molecular dynamics simulations of a model membrane bilayer.

Techniques: In Vitro, Concentration Assay, Activity Assay, Liposomes, Sedimentation

Journal: Molecular Cell

Article Title: Osh Proteins Control Nanoscale Lipid Organization Necessary for PI(4,5)P 2 Synthesis

doi: 10.1016/j.molcel.2019.06.037

Figure Lengend Snippet: Molecular View of the PIP5K Specificity Loop Embedded in a Lipid Bilayer (A–C) The specificity loop of PIP5K (5K loop ) embedded into a model membrane bilayer in atomistic molecular dynamics simulations. The 5K loop is shown as ribbon (orange) with hydrophobic, polar, acidic, and basic amino acids represented as sticks in white, green, red, and blue, respectively. The surfaces of lipids and sterols are shown for POPC (gray), DOPS (magenta), cholesterol (cyan), and bPI4P (yellow). The figure shows an area of the membrane leaflet of approximately 4 × 4 nm (A). Cross sectional views of the 5K loop embedded in the model membrane bilayer (B and C). Water and ions outside the membrane are omitted for clarity. (D–G) Lipid localization. Time-averaged positions of the phosphate moieties of the phospholipids POPC (D), DOPS (E), and PI4P (G) or oxygen atoms in cholesterol (Chol, F) from all-atom molecular dynamics simulations of a model membrane bilayer. Colors indicate the localization probability of different lipids over the course of the whole 10-μs trajectory. The membrane leaflet (cytoplasmic) with the 5K loop embedded is shown in the left panels; the opposing leaflet (extracellular) is shown on the right. Scale bars, 2.5 nm. (H–J) Atomistic views of the bPI4P molecule interacting with pocket #1 of the 5K loop with a cholesterol molecule on the other side as shown in Figure 7 B (H); 90° orientation (I); 180° orientation (J). The two phosphate moieties of bPI4P interact with four basic residues of the amphipathic helix (R384, K387, K388, and H391). The cholesterol hydrocarbon chain wedges between the acyl chains of the polyunsaturated PI4P. The amphipathic helix is visualized as a ribbon (orange) and acidic and basic residues as sticks in green. Positively charged nitrogen atoms and negatively charged oxygen atoms are shown as spheres, colored blue and red, respectively. See also Figure S7 .

Article Snippet: Time-averaged positions of the phosphate moieties of the phospholipids POPC (D), DOPS (E), and PI4P (G) or oxygen atoms in cholesterol (Chol, F) from all-atom molecular dynamics simulations of a model membrane bilayer.

Techniques: Membrane

Journal: Science Advances

Article Title: From synthetic vesicles to living cells: Anchoring conducting polymers to cell membrane

doi: 10.1126/sciadv.adr2882

Figure Lengend Snippet: Absorbance ( A ) and emission (excitation 350 nm) ( B ) spectra of ETE-PEGO:POPC vesicles before (solid lines) and after (dashed lines) enzymatic polymerization with HRP and H 2 O 2 , with (purple) and without (green) ETE-S. ( C ) Bright-field and fluorescence microscopy of ETE-PEGO:POPC/DOPS giant vesicles before (left, ETE-PEGO:POPC/DOPS) and after [right, p(ETE-PEGO POPC/DOPS :ETE-S)] enzymatic polymerization with HRP and H 2 O 2 , in the presence of ETE-S (scale bar, 50 μm). Giant vesicles are marked in the images (see also fig. S14 for an enlarged version) ( D ) Current-voltage characteristics of p(ETE-S) (blue) and p(ETE-PEGO POPC :ETE-S) (purple). ( E ) Height image and corresponding current map of p(ETE-PEGO POPC :ETE-S). ( F ) Height image and corresponding current map of p(ETE-S).

Article Snippet: H 2 O 2 and HRP (type I, ≥ 50 U/mg solid), POPC, DOPS, d -sorbitol, and chloroform were purchased from Merck.

Techniques: Fluorescence, Microscopy