Journal: Frontiers in Pharmacology

Article Title: Enhanced therapeutic potential of paeoniflorin and vitamin B12 in intracerebropeduncle ethidium bromide-induced multiple sclerosis-like pathology

doi: 10.3389/fphar.2026.1792674

Figure Lengend Snippet: (A–E) PNN neuroprotective role in mitigating EBRO-induced alterations in neurotransmitter levels, including Acetylcholine (A) ; Dopamine (B) ; GABA (C) ; Glutamate (D) , and Serotonin (E) in brain homogenates. To identify significant differences between groups, a one-way ANOVA and Tukey’s post hoc test were used for statistical analysis (A–D) . The statistical significance level was set at p < 0.01, and the data were displayed as mean ± standard deviation (SD). There were eight wistar rats (n = 8) in each experimental group. β v/s Sham Control, Vehicle Control, and PNN Perse; δ v/s EBRO; δα1 v/s EBRO + PNN50; δα2 v/s EBRO + PNN100, EBRO + PNN50; and δα3 v/s EBRO + VB12 (30), EBRO + PNN100, EBRO + PNN50.

Article Snippet: Additionally, neurotransmitters like dopamine [KLR0219; Krishgen Biosystem, Mumbai, India], glutamate [KLR1474; Krishgen Biosystem, Mumbai, India], serotonin [KLR0866; Krishgen Biosystem, Mumbai, India], acetylcholine [KLR0722; Krishgen Biosystem, Mumbai, India] , and GABA [KLR0102; Krishgen Biosystem, Mumbai, India] were evaluated ( ).

Techniques: Standard Deviation, Control

Journal:

Article Title:

doi:

Figure Lengend Snippet: Expression of dopamine transporter (DAT), norepinephrine transporter (NET), and serotonin transporter (SERT) in astroglial cultures prepared from Sprague-Dawley newborn cortex (a), (c), (e) or embryonic striatum (b), (d), (f). Confocal laser-scanning microscopic images showing double immunostaining for DAT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (a) and (b)), NET (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (c) and (d)), or SERT (fluorescein-isothiocyanate, green) and GFAP (rhodamine, red) with nuclear staining (4′,6-diamino-2-phenylindole, blue; (e) and (f)) in cortical or striatal astroglia prepared from Sprague-Dawley rats. Scale bar = 20 µm.

Article Snippet: Cells were incubated with the primary antibodies (mouse anti-DAT [1:100; Alomone Labs, #AMT-003], anti-NET [1:100; Alomone Labs, #AMT-002], or rabbit ant-SERT [1:200; Chemicon International, MAB1564] antibody, mouse anti-GFAP [1:200; Sigma-Aldrich, #G3893], or rabbit anti-GFAP [1:200; DAKO, #Z0334]) for 2 hr at room temperature.

Techniques: Expressing, Double Immunostaining, Staining

Journal:

Article Title:

doi:

Figure Lengend Snippet: Effects of the simultaneous inhibition of three Na + -dependent monoamine transporters (DAT, NET, and SERT) on dopamine (DA)-induced increases in pentose-phosphate pathway (PPP) flux in cortical (a) and striatal (b) astroglia. Values are the mean ± SD of four flasks. * p < .05 (analysis of variance followed by Tukey test for multiple comparisons). DA = dopamine; ns = not significant.

Article Snippet: Cells were incubated with the primary antibodies (mouse anti-DAT [1:100; Alomone Labs, #AMT-003], anti-NET [1:100; Alomone Labs, #AMT-002], or rabbit ant-SERT [1:200; Chemicon International, MAB1564] antibody, mouse anti-GFAP [1:200; Sigma-Aldrich, #G3893], or rabbit anti-GFAP [1:200; DAKO, #Z0334]) for 2 hr at room temperature.

Techniques: Inhibition

Journal: Cell Reports Medicine

Article Title: 5-HT reuptake blockade induces pyroptosis in BRAF V600E -mutated melanomas via remodeling histone serotonylation

doi: 10.1016/j.xcrm.2025.102537

Figure Lengend Snippet: High-throughput drug screening reveals PH as an alternative therapeutic for BRAF V600E -mutant melanoma (A) Waterfall plots showing the effects of an antidepressant compound library containing 90 drugs. Inhibition rates >85% drugs (green). Three biological replicates were screened for each sample. (B) IC 50 of PH in the indicated cell lines. (C) Colony formation assays in the indicated cells treated with DMSO or gradient doses of PH. (D) A375 xenografts were established and treated with vehicle or PH (25 mg/kg daily intraperitoneally [i.p.]) for 14 days. Tumors in each group were individually recorded every 2 days. n = 8 tumors per group. (E and F) Weight (E) and image (F) of xenograft tumors in (D). (G) 5-HT levels in A375 cells treated with DMSO or the indicated PH treatment for 24 h. (H) 5-HT levels in A375 cells with the indicated treatment. (I and J) CCK8 (I) and clonogenic (J) assays in A375 cells with the indicated treatment. Data in (D) and (E) are displayed as mean ± SEM, and the others are displayed as mean ± SD. Statistical significance was determined using Student’s t test. ∗∗∗ p < 0.001, ∗∗ p < 0.01, ∗ p < 0.05. See also .

Article Snippet: Antidepressant Compound Custom Library , TargetMol , Rack ID:PHD163731/PHD163732/PHD163733.

Techniques: High Throughput Screening Assay, Drug discovery, Mutagenesis, Inhibition