donkey anti-goat igg Search Results


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  • 98
    LI-COR donkey antigoat igg irdye 800cw
    Expression and purification of the LF8-Fab. (a) A goat antihuman Fab and <t>IRDye</t> <t>800CW</t> donkey <t>antigoat</t> <t>IgG</t> were used to detect Fab expression in Western blotting. Both heavy chain Fd (Fd) and light chain (L) were expressed. Lane 1, supernatant of sonicated lysate; lane 2, pellet of sonicated lysate; lane 3, lysate of untranfected E. coli HB2151 as negative control; lane 4, protein marker. (b) Coomassie blue staining showed that heavy chain Fd (Fd) and light chain (L) of the LF8-Fab were equally expressed after the purification of IMAC and IEC.
    Donkey Antigoat Igg Irdye 800cw, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher donkey antigoat igg
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of <t>antigoat</t> <t>IgG</t> (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Donkey Antigoat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Jackson Immuno donkey antigoat igg
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of <t>antigoat</t> <t>IgG</t> (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Donkey Antigoat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam donkey antigoat igg conjugated
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of <t>antigoat</t> <t>IgG</t> (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Donkey Antigoat Igg Conjugated, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    R&D Systems donkey antigoat igg nl637 antibodies
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of <t>antigoat</t> <t>IgG</t> (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Donkey Antigoat Igg Nl637 Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam alexa fluor 647 donkey antigoat igg
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of <t>antigoat</t> <t>IgG</t> (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Alexa Fluor 647 Donkey Antigoat Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher fitc labeled donkey antigoat igg
    Identification of choline acetyltransferase (ChAT) expression on human cerebral microvascular endothelial cells (HCMECs) by immunocytochemistry. HCMECs were fixed and continued with incubation using primer antibody goat anti ChAT (Clone AB144P) and goat anti human platelet and endothelial cell adhesion molecule 1 (PECAM-1) (Clone SC 1506). After overnight incubation, cells were washed and labeled by secondary antibody (Alexa fluor <t>FITC</t> labeled donkey anti goat or PE donkey anti goat immunoglobulin G), nucleus were stained with 4’,6-diamidino-2-phenylindole. Blue=Nucleus; Green=ChAT; Red=PECAM-1.
    Fitc Labeled Donkey Antigoat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Abcam horseradish peroxidase conjugated donkey antigoat igg
    Identification of choline acetyltransferase (ChAT) expression on human cerebral microvascular endothelial cells (HCMECs) by immunocytochemistry. HCMECs were fixed and continued with incubation using primer antibody goat anti ChAT (Clone AB144P) and goat anti human platelet and endothelial cell adhesion molecule 1 (PECAM-1) (Clone SC 1506). After overnight incubation, cells were washed and labeled by secondary antibody (Alexa fluor <t>FITC</t> labeled donkey anti goat or PE donkey anti goat immunoglobulin G), nucleus were stained with 4’,6-diamidino-2-phenylindole. Blue=Nucleus; Green=ChAT; Red=PECAM-1.
    Horseradish Peroxidase Conjugated Donkey Antigoat Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Abcam antigoat alexa488
    Identification of choline acetyltransferase (ChAT) expression on human cerebral microvascular endothelial cells (HCMECs) by immunocytochemistry. HCMECs were fixed and continued with incubation using primer antibody goat anti ChAT (Clone AB144P) and goat anti human platelet and endothelial cell adhesion molecule 1 (PECAM-1) (Clone SC 1506). After overnight incubation, cells were washed and labeled by secondary antibody (Alexa fluor <t>FITC</t> labeled donkey anti goat or PE donkey anti goat immunoglobulin G), nucleus were stained with 4’,6-diamidino-2-phenylindole. Blue=Nucleus; Green=ChAT; Red=PECAM-1.
    Antigoat Alexa488, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega donkey anti goat igg ap conjugate
    Identification of choline acetyltransferase (ChAT) expression on human cerebral microvascular endothelial cells (HCMECs) by immunocytochemistry. HCMECs were fixed and continued with incubation using primer antibody goat anti ChAT (Clone AB144P) and goat anti human platelet and endothelial cell adhesion molecule 1 (PECAM-1) (Clone SC 1506). After overnight incubation, cells were washed and labeled by secondary antibody (Alexa fluor <t>FITC</t> labeled donkey anti goat or PE donkey anti goat immunoglobulin G), nucleus were stained with 4’,6-diamidino-2-phenylindole. Blue=Nucleus; Green=ChAT; Red=PECAM-1.
    Donkey Anti Goat Igg Ap Conjugate, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega donkey anti goat igg hrp
    Identification of choline acetyltransferase (ChAT) expression on human cerebral microvascular endothelial cells (HCMECs) by immunocytochemistry. HCMECs were fixed and continued with incubation using primer antibody goat anti ChAT (Clone AB144P) and goat anti human platelet and endothelial cell adhesion molecule 1 (PECAM-1) (Clone SC 1506). After overnight incubation, cells were washed and labeled by secondary antibody (Alexa fluor <t>FITC</t> labeled donkey anti goat or PE donkey anti goat immunoglobulin G), nucleus were stained with 4’,6-diamidino-2-phenylindole. Blue=Nucleus; Green=ChAT; Red=PECAM-1.
    Donkey Anti Goat Igg Hrp, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology donkey anti goat igg
    K8/K18 physically interacts with DR5 (A) Cell lysates from MCF7 and T47D cell lines were subjected to immunoprecipitation (IP) with an anti-K8/K18 antibody immobilized to protein A-agarose (lane 2 and 4) or with protein A-agarose alone (lane 1 and 3). IP samples were immunoblotted for DR5. (B) Cells were fixed, permeabilized, stained with anti-DR5 antibody (red) and anti-keratin 8 antibody (green), and analyzed by confocal microscopy. All images were acquired using a 40x objective lens. (C) MCF7 cells were transfected with control siRNA or siRNA against KRT8 for 72 hr. The resultant cells were fixed, permeabilized, and stained with anti-DR5 antibody (red), DAPI (nuclei, blue), and fluorescent phalloidin 488 for actin visualization (green). All images were acquired using a 40x objective lens. (D-F) Flow cytometry analysis of cells transfected with control siRNA or siRNA against KRT8 . The resultant cells were harvested using a non-enzymatic cell dissociation solution. The cell samples were incubated with PE conjugated anti-DR5 antibody <t>(IgG2b)</t> or its corresponding IgG2b isotype as a control. (D) Shown are representative histograms of unstained cells (black dashed line), cells stained with the isotype control (red fill), and cells stained with PE-anti-DR5 antibody (blue outline with no fill). The right shift represents the presence of DR5 on the cell surface membrane. Median PE-anti-DR5 antibody fluorescence intensity for cells transfected with control siRNA and siRNA targeting keratin 8 are shown after subtraction of isotype control median fluorescence intensity in two independent experiments for T47D cells (E) and MCF7 cells (F).
    Donkey Anti Goat Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    The Jackson Laboratory donkey anti goat igg
    K8/K18 physically interacts with DR5 (A) Cell lysates from MCF7 and T47D cell lines were subjected to immunoprecipitation (IP) with an anti-K8/K18 antibody immobilized to protein A-agarose (lane 2 and 4) or with protein A-agarose alone (lane 1 and 3). IP samples were immunoblotted for DR5. (B) Cells were fixed, permeabilized, stained with anti-DR5 antibody (red) and anti-keratin 8 antibody (green), and analyzed by confocal microscopy. All images were acquired using a 40x objective lens. (C) MCF7 cells were transfected with control siRNA or siRNA against KRT8 for 72 hr. The resultant cells were fixed, permeabilized, and stained with anti-DR5 antibody (red), DAPI (nuclei, blue), and fluorescent phalloidin 488 for actin visualization (green). All images were acquired using a 40x objective lens. (D-F) Flow cytometry analysis of cells transfected with control siRNA or siRNA against KRT8 . The resultant cells were harvested using a non-enzymatic cell dissociation solution. The cell samples were incubated with PE conjugated anti-DR5 antibody <t>(IgG2b)</t> or its corresponding IgG2b isotype as a control. (D) Shown are representative histograms of unstained cells (black dashed line), cells stained with the isotype control (red fill), and cells stained with PE-anti-DR5 antibody (blue outline with no fill). The right shift represents the presence of DR5 on the cell surface membrane. Median PE-anti-DR5 antibody fluorescence intensity for cells transfected with control siRNA and siRNA targeting keratin 8 are shown after subtraction of isotype control median fluorescence intensity in two independent experiments for T47D cells (E) and MCF7 cells (F).
    Donkey Anti Goat Igg, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 92/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Merck & Co donkey anti goat igg
    K8/K18 physically interacts with DR5 (A) Cell lysates from MCF7 and T47D cell lines were subjected to immunoprecipitation (IP) with an anti-K8/K18 antibody immobilized to protein A-agarose (lane 2 and 4) or with protein A-agarose alone (lane 1 and 3). IP samples were immunoblotted for DR5. (B) Cells were fixed, permeabilized, stained with anti-DR5 antibody (red) and anti-keratin 8 antibody (green), and analyzed by confocal microscopy. All images were acquired using a 40x objective lens. (C) MCF7 cells were transfected with control siRNA or siRNA against KRT8 for 72 hr. The resultant cells were fixed, permeabilized, and stained with anti-DR5 antibody (red), DAPI (nuclei, blue), and fluorescent phalloidin 488 for actin visualization (green). All images were acquired using a 40x objective lens. (D-F) Flow cytometry analysis of cells transfected with control siRNA or siRNA against KRT8 . The resultant cells were harvested using a non-enzymatic cell dissociation solution. The cell samples were incubated with PE conjugated anti-DR5 antibody <t>(IgG2b)</t> or its corresponding IgG2b isotype as a control. (D) Shown are representative histograms of unstained cells (black dashed line), cells stained with the isotype control (red fill), and cells stained with PE-anti-DR5 antibody (blue outline with no fill). The right shift represents the presence of DR5 on the cell surface membrane. Median PE-anti-DR5 antibody fluorescence intensity for cells transfected with control siRNA and siRNA targeting keratin 8 are shown after subtraction of isotype control median fluorescence intensity in two independent experiments for T47D cells (E) and MCF7 cells (F).
    Donkey Anti Goat Igg, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Cell Signaling Technology Inc donkey anti goat igg
    K8/K18 physically interacts with DR5 (A) Cell lysates from MCF7 and T47D cell lines were subjected to immunoprecipitation (IP) with an anti-K8/K18 antibody immobilized to protein A-agarose (lane 2 and 4) or with protein A-agarose alone (lane 1 and 3). IP samples were immunoblotted for DR5. (B) Cells were fixed, permeabilized, stained with anti-DR5 antibody (red) and anti-keratin 8 antibody (green), and analyzed by confocal microscopy. All images were acquired using a 40x objective lens. (C) MCF7 cells were transfected with control siRNA or siRNA against KRT8 for 72 hr. The resultant cells were fixed, permeabilized, and stained with anti-DR5 antibody (red), DAPI (nuclei, blue), and fluorescent phalloidin 488 for actin visualization (green). All images were acquired using a 40x objective lens. (D-F) Flow cytometry analysis of cells transfected with control siRNA or siRNA against KRT8 . The resultant cells were harvested using a non-enzymatic cell dissociation solution. The cell samples were incubated with PE conjugated anti-DR5 antibody <t>(IgG2b)</t> or its corresponding IgG2b isotype as a control. (D) Shown are representative histograms of unstained cells (black dashed line), cells stained with the isotype control (red fill), and cells stained with PE-anti-DR5 antibody (blue outline with no fill). The right shift represents the presence of DR5 on the cell surface membrane. Median PE-anti-DR5 antibody fluorescence intensity for cells transfected with control siRNA and siRNA targeting keratin 8 are shown after subtraction of isotype control median fluorescence intensity in two independent experiments for T47D cells (E) and MCF7 cells (F).
    Donkey Anti Goat Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare donkey anti goat igg
    K8/K18 physically interacts with DR5 (A) Cell lysates from MCF7 and T47D cell lines were subjected to immunoprecipitation (IP) with an anti-K8/K18 antibody immobilized to protein A-agarose (lane 2 and 4) or with protein A-agarose alone (lane 1 and 3). IP samples were immunoblotted for DR5. (B) Cells were fixed, permeabilized, stained with anti-DR5 antibody (red) and anti-keratin 8 antibody (green), and analyzed by confocal microscopy. All images were acquired using a 40x objective lens. (C) MCF7 cells were transfected with control siRNA or siRNA against KRT8 for 72 hr. The resultant cells were fixed, permeabilized, and stained with anti-DR5 antibody (red), DAPI (nuclei, blue), and fluorescent phalloidin 488 for actin visualization (green). All images were acquired using a 40x objective lens. (D-F) Flow cytometry analysis of cells transfected with control siRNA or siRNA against KRT8 . The resultant cells were harvested using a non-enzymatic cell dissociation solution. The cell samples were incubated with PE conjugated anti-DR5 antibody <t>(IgG2b)</t> or its corresponding IgG2b isotype as a control. (D) Shown are representative histograms of unstained cells (black dashed line), cells stained with the isotype control (red fill), and cells stained with PE-anti-DR5 antibody (blue outline with no fill). The right shift represents the presence of DR5 on the cell surface membrane. Median PE-anti-DR5 antibody fluorescence intensity for cells transfected with control siRNA and siRNA targeting keratin 8 are shown after subtraction of isotype control median fluorescence intensity in two independent experiments for T47D cells (E) and MCF7 cells (F).
    Donkey Anti Goat Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Expression and purification of the LF8-Fab. (a) A goat antihuman Fab and IRDye 800CW donkey antigoat IgG were used to detect Fab expression in Western blotting. Both heavy chain Fd (Fd) and light chain (L) were expressed. Lane 1, supernatant of sonicated lysate; lane 2, pellet of sonicated lysate; lane 3, lysate of untranfected E. coli HB2151 as negative control; lane 4, protein marker. (b) Coomassie blue staining showed that heavy chain Fd (Fd) and light chain (L) of the LF8-Fab were equally expressed after the purification of IMAC and IEC.

    Journal: Clinical and Developmental Immunology

    Article Title: A Human/Murine Chimeric Fab Antibody Neutralizes Anthrax Lethal Toxin In Vitro

    doi: 10.1155/2013/475809

    Figure Lengend Snippet: Expression and purification of the LF8-Fab. (a) A goat antihuman Fab and IRDye 800CW donkey antigoat IgG were used to detect Fab expression in Western blotting. Both heavy chain Fd (Fd) and light chain (L) were expressed. Lane 1, supernatant of sonicated lysate; lane 2, pellet of sonicated lysate; lane 3, lysate of untranfected E. coli HB2151 as negative control; lane 4, protein marker. (b) Coomassie blue staining showed that heavy chain Fd (Fd) and light chain (L) of the LF8-Fab were equally expressed after the purification of IMAC and IEC.

    Article Snippet: Individual clones were identified by Western blotting using goat antihuman IgG (Fab-specific) and IRDye 800CW donkey antigoat IgG in an Odyssey infrared image system (LI-COR Biosciences, Lincoln, NE).

    Techniques: Expressing, Purification, Western Blot, Sonication, Negative Control, Marker, Staining

    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat IgG (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)

    Journal: Experimental Biology and Medicine

    Article Title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct

    doi: 10.1177/1535370216673746

    Figure Lengend Snippet: Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat IgG (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)

    Article Snippet: Following washing with PBS, the slices were incubated with secondary antibodies of donkey antigoat IgG (Alexa Fluor® 594-conjugated; Invitrogen), and donkey antirabbit IgG (Alexa Fluor® 488-conjugated; Invitrogen), and goat antimouse IgG (Alexa Fluor® 594-conjugated; Invitrogen) for 30 min at room temperature in the humidified chamber.

    Techniques: Fluorescence, Staining, Negative Control

    Representative images of light microscopic observation and immuno-fluorescence staining of primary cultured porcine thyroid cells. (a) The monolayer porcine thyroid cells in culture after two days of incubation. (b) The thyroid cells with the hemisphere-like domes formed in culture after four days of incubation. (a and b) Scale bar = 100 µm. (c) Negative control for antirabbit IgG. (d) Negative control for antigoat IgG. (e) Positive staining of sodium-iodide symporter (NIS). (f) Positive staining of thyroglobulin (Tg). (g) Composite picture of NIS, Tg, and DAPI. (c to g) Scale bar = 50 µm. (A color version of this figure is available in the online journal.)

    Journal: Experimental Biology and Medicine

    Article Title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct

    doi: 10.1177/1535370216673746

    Figure Lengend Snippet: Representative images of light microscopic observation and immuno-fluorescence staining of primary cultured porcine thyroid cells. (a) The monolayer porcine thyroid cells in culture after two days of incubation. (b) The thyroid cells with the hemisphere-like domes formed in culture after four days of incubation. (a and b) Scale bar = 100 µm. (c) Negative control for antirabbit IgG. (d) Negative control for antigoat IgG. (e) Positive staining of sodium-iodide symporter (NIS). (f) Positive staining of thyroglobulin (Tg). (g) Composite picture of NIS, Tg, and DAPI. (c to g) Scale bar = 50 µm. (A color version of this figure is available in the online journal.)

    Article Snippet: Following washing with PBS, the slices were incubated with secondary antibodies of donkey antigoat IgG (Alexa Fluor® 594-conjugated; Invitrogen), and donkey antirabbit IgG (Alexa Fluor® 488-conjugated; Invitrogen), and goat antimouse IgG (Alexa Fluor® 594-conjugated; Invitrogen) for 30 min at room temperature in the humidified chamber.

    Techniques: Fluorescence, Staining, Cell Culture, Incubation, Negative Control

    Identification of choline acetyltransferase (ChAT) expression on human cerebral microvascular endothelial cells (HCMECs) by immunocytochemistry. HCMECs were fixed and continued with incubation using primer antibody goat anti ChAT (Clone AB144P) and goat anti human platelet and endothelial cell adhesion molecule 1 (PECAM-1) (Clone SC 1506). After overnight incubation, cells were washed and labeled by secondary antibody (Alexa fluor FITC labeled donkey anti goat or PE donkey anti goat immunoglobulin G), nucleus were stained with 4’,6-diamidino-2-phenylindole. Blue=Nucleus; Green=ChAT; Red=PECAM-1.

    Journal: Veterinary World

    Article Title: Ocimum sanctum Linn. stimulate the expression of choline acetyltransferase on the human cerebral microvascular endothelial cells

    doi: 10.14202/vetworld.2016.1348-1354

    Figure Lengend Snippet: Identification of choline acetyltransferase (ChAT) expression on human cerebral microvascular endothelial cells (HCMECs) by immunocytochemistry. HCMECs were fixed and continued with incubation using primer antibody goat anti ChAT (Clone AB144P) and goat anti human platelet and endothelial cell adhesion molecule 1 (PECAM-1) (Clone SC 1506). After overnight incubation, cells were washed and labeled by secondary antibody (Alexa fluor FITC labeled donkey anti goat or PE donkey anti goat immunoglobulin G), nucleus were stained with 4’,6-diamidino-2-phenylindole. Blue=Nucleus; Green=ChAT; Red=PECAM-1.

    Article Snippet: Labeled cells were visualized using FITC-labeled rabbit-antimouse IgG (Dako) or FITC-labeled donkey antigoat IgG (Thermo Scientific) and PE-labeled donkey antigoat IgG (Dako).

    Techniques: Expressing, Immunocytochemistry, Incubation, Labeling, Staining

    K8/K18 physically interacts with DR5 (A) Cell lysates from MCF7 and T47D cell lines were subjected to immunoprecipitation (IP) with an anti-K8/K18 antibody immobilized to protein A-agarose (lane 2 and 4) or with protein A-agarose alone (lane 1 and 3). IP samples were immunoblotted for DR5. (B) Cells were fixed, permeabilized, stained with anti-DR5 antibody (red) and anti-keratin 8 antibody (green), and analyzed by confocal microscopy. All images were acquired using a 40x objective lens. (C) MCF7 cells were transfected with control siRNA or siRNA against KRT8 for 72 hr. The resultant cells were fixed, permeabilized, and stained with anti-DR5 antibody (red), DAPI (nuclei, blue), and fluorescent phalloidin 488 for actin visualization (green). All images were acquired using a 40x objective lens. (D-F) Flow cytometry analysis of cells transfected with control siRNA or siRNA against KRT8 . The resultant cells were harvested using a non-enzymatic cell dissociation solution. The cell samples were incubated with PE conjugated anti-DR5 antibody (IgG2b) or its corresponding IgG2b isotype as a control. (D) Shown are representative histograms of unstained cells (black dashed line), cells stained with the isotype control (red fill), and cells stained with PE-anti-DR5 antibody (blue outline with no fill). The right shift represents the presence of DR5 on the cell surface membrane. Median PE-anti-DR5 antibody fluorescence intensity for cells transfected with control siRNA and siRNA targeting keratin 8 are shown after subtraction of isotype control median fluorescence intensity in two independent experiments for T47D cells (E) and MCF7 cells (F).

    Journal: Oncotarget

    Article Title: Cytokeratin 8/18 protects breast cancer cell lines from TRAIL-induced apoptosis

    doi: 10.18632/oncotarget.25297

    Figure Lengend Snippet: K8/K18 physically interacts with DR5 (A) Cell lysates from MCF7 and T47D cell lines were subjected to immunoprecipitation (IP) with an anti-K8/K18 antibody immobilized to protein A-agarose (lane 2 and 4) or with protein A-agarose alone (lane 1 and 3). IP samples were immunoblotted for DR5. (B) Cells were fixed, permeabilized, stained with anti-DR5 antibody (red) and anti-keratin 8 antibody (green), and analyzed by confocal microscopy. All images were acquired using a 40x objective lens. (C) MCF7 cells were transfected with control siRNA or siRNA against KRT8 for 72 hr. The resultant cells were fixed, permeabilized, and stained with anti-DR5 antibody (red), DAPI (nuclei, blue), and fluorescent phalloidin 488 for actin visualization (green). All images were acquired using a 40x objective lens. (D-F) Flow cytometry analysis of cells transfected with control siRNA or siRNA against KRT8 . The resultant cells were harvested using a non-enzymatic cell dissociation solution. The cell samples were incubated with PE conjugated anti-DR5 antibody (IgG2b) or its corresponding IgG2b isotype as a control. (D) Shown are representative histograms of unstained cells (black dashed line), cells stained with the isotype control (red fill), and cells stained with PE-anti-DR5 antibody (blue outline with no fill). The right shift represents the presence of DR5 on the cell surface membrane. Median PE-anti-DR5 antibody fluorescence intensity for cells transfected with control siRNA and siRNA targeting keratin 8 are shown after subtraction of isotype control median fluorescence intensity in two independent experiments for T47D cells (E) and MCF7 cells (F).

    Article Snippet: Horseradish peroxidase–conjugated goat anti-rabbit IgG1 (sc-2054), goat anti-mouse IgG1 (sc-2969), and donkey anti-goat IgG (sc-2020) were purchased from Santa Cruz Biotechnology.

    Techniques: Immunoprecipitation, Staining, Confocal Microscopy, Transfection, Flow Cytometry, Cytometry, Incubation, Fluorescence