Journal: Data in Brief

Article Title: Shotgun metagenomics sequencing data of root microbial community of Huanglongbing-infected Citrus nobilis

doi: 10.1016/j.dib.2024.111061

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Article Snippet: Data collection , Root samples of HLB-infected C. nobilis L., which showed Huanglongbing symptoms in leaves, were collected from three positions in Dak Lak. The surface of the roots was sterilized and used to isolate the genomic DNA using DNeasy PowerSoil Pro kit. The shotgun metagenomic library was created using the NEBNext Ultra II DNA Library Prep Kit for Illumina. The DNBSeq-G99 machine was utilized to sequence the shotgun metagenomic library with the Illumina platform. Bcl2fastq 2.20 was used to demultiplex the raw data. Trimmomatic 0.39 and Cutadapt 2.10 were used to filter the sequence data. Kraken2 was used to assess taxonomic profiles, and the MetaCyc database was utilized to analyze functional characteristics..

Techniques: Sequencing, Functional Assay