dnase-free Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Millipore dnase free
    Dnase Free, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free/product/Millipore
    Average 98 stars, based on 95 article reviews
    Price from $9.99 to $1999.99
    dnase free - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

    99
    Millipore dnase free rnase
    DAPI-positivity of tau deposits is not affected by <t>RNase</t> A and/or <t>DNase</t> I treatments. (A and E) DNase I (–)/RNase A (–); (B and F) DNase I (+)/RNase A (–); (C and G) DNase I (–)/RNase A (+); (D and H) DNase I (+)/RNase A (+). (A–D) All sections were double-stained with ThS (green) and DAPI (blue). (E–H) All sections were stained with DAPI (blue) alone. Calibration bars = 10 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; ThS, thioflavin-S.
    Dnase Free Rnase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free rnase/product/Millipore
    Average 99 stars, based on 1284 article reviews
    Price from $9.99 to $1999.99
    dnase free rnase - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    90
    Boehringer Mannheim dnase free
    DAPI-positivity of tau deposits is not affected by <t>RNase</t> A and/or <t>DNase</t> I treatments. (A and E) DNase I (–)/RNase A (–); (B and F) DNase I (+)/RNase A (–); (C and G) DNase I (–)/RNase A (+); (D and H) DNase I (+)/RNase A (+). (A–D) All sections were double-stained with ThS (green) and DAPI (blue). (E–H) All sections were stained with DAPI (blue) alone. Calibration bars = 10 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; ThS, thioflavin-S.
    Dnase Free, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 90/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free/product/Boehringer Mannheim
    Average 90 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    dnase free - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    92
    Roche dnaase free
    DAPI-positivity of tau deposits is not affected by <t>RNase</t> A and/or <t>DNase</t> I treatments. (A and E) DNase I (–)/RNase A (–); (B and F) DNase I (+)/RNase A (–); (C and G) DNase I (–)/RNase A (+); (D and H) DNase I (+)/RNase A (+). (A–D) All sections were double-stained with ThS (green) and DAPI (blue). (E–H) All sections were stained with DAPI (blue) alone. Calibration bars = 10 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; ThS, thioflavin-S.
    Dnaase Free, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnaase free/product/Roche
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    dnaase free - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Millipore rnase free dnase
    DAPI-positivity of tau deposits is not affected by <t>RNase</t> A and/or <t>DNase</t> I treatments. (A and E) DNase I (–)/RNase A (–); (B and F) DNase I (+)/RNase A (–); (C and G) DNase I (–)/RNase A (+); (D and H) DNase I (+)/RNase A (+). (A–D) All sections were double-stained with ThS (green) and DAPI (blue). (E–H) All sections were stained with DAPI (blue) alone. Calibration bars = 10 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; ThS, thioflavin-S.
    Rnase Free Dnase, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase free dnase/product/Millipore
    Average 93 stars, based on 327 article reviews
    Price from $9.99 to $1999.99
    rnase free dnase - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    89
    Thermo Fisher dnase free kit
    DAPI-positivity of tau deposits is not affected by <t>RNase</t> A and/or <t>DNase</t> I treatments. (A and E) DNase I (–)/RNase A (–); (B and F) DNase I (+)/RNase A (–); (C and G) DNase I (–)/RNase A (+); (D and H) DNase I (+)/RNase A (+). (A–D) All sections were double-stained with ThS (green) and DAPI (blue). (E–H) All sections were stained with DAPI (blue) alone. Calibration bars = 10 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; ThS, thioflavin-S.
    Dnase Free Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free kit/product/Thermo Fisher
    Average 89 stars, based on 259 article reviews
    Price from $9.99 to $1999.99
    dnase free kit - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    89
    Roche dnase free rnasea
    DAPI-positivity of tau deposits is not affected by <t>RNase</t> A and/or <t>DNase</t> I treatments. (A and E) DNase I (–)/RNase A (–); (B and F) DNase I (+)/RNase A (–); (C and G) DNase I (–)/RNase A (+); (D and H) DNase I (+)/RNase A (+). (A–D) All sections were double-stained with ThS (green) and DAPI (blue). (E–H) All sections were stained with DAPI (blue) alone. Calibration bars = 10 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; ThS, thioflavin-S.
    Dnase Free Rnasea, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free rnasea/product/Roche
    Average 89 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    dnase free rnasea - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    85
    Fisher Scientific dnaase free tubes
    DAPI-positivity of tau deposits is not affected by <t>RNase</t> A and/or <t>DNase</t> I treatments. (A and E) DNase I (–)/RNase A (–); (B and F) DNase I (+)/RNase A (–); (C and G) DNase I (–)/RNase A (+); (D and H) DNase I (+)/RNase A (+). (A–D) All sections were double-stained with ThS (green) and DAPI (blue). (E–H) All sections were stained with DAPI (blue) alone. Calibration bars = 10 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; ThS, thioflavin-S.
    Dnaase Free Tubes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnaase free tubes/product/Fisher Scientific
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    dnaase free tubes - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    91
    Roche dnase free kit
    DAPI-positivity of tau deposits is not affected by <t>RNase</t> A and/or <t>DNase</t> I treatments. (A and E) DNase I (–)/RNase A (–); (B and F) DNase I (+)/RNase A (–); (C and G) DNase I (–)/RNase A (+); (D and H) DNase I (+)/RNase A (+). (A–D) All sections were double-stained with ThS (green) and DAPI (blue). (E–H) All sections were stained with DAPI (blue) alone. Calibration bars = 10 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; ThS, thioflavin-S.
    Dnase Free Kit, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free kit/product/Roche
    Average 91 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    dnase free kit - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    88
    GE Healthcare dnase free rnaase
    DAPI-positivity of tau deposits is not affected by <t>RNase</t> A and/or <t>DNase</t> I treatments. (A and E) DNase I (–)/RNase A (–); (B and F) DNase I (+)/RNase A (–); (C and G) DNase I (–)/RNase A (+); (D and H) DNase I (+)/RNase A (+). (A–D) All sections were double-stained with ThS (green) and DAPI (blue). (E–H) All sections were stained with DAPI (blue) alone. Calibration bars = 10 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; ThS, thioflavin-S.
    Dnase Free Rnaase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free rnaase/product/GE Healthcare
    Average 88 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    dnase free rnaase - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    90
    Eppendorf AG dnase free
    DAPI-positivity of tau deposits is not affected by <t>RNase</t> A and/or <t>DNase</t> I treatments. (A and E) DNase I (–)/RNase A (–); (B and F) DNase I (+)/RNase A (–); (C and G) DNase I (–)/RNase A (+); (D and H) DNase I (+)/RNase A (+). (A–D) All sections were double-stained with ThS (green) and DAPI (blue). (E–H) All sections were stained with DAPI (blue) alone. Calibration bars = 10 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; ThS, thioflavin-S.
    Dnase Free, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free/product/Eppendorf AG
    Average 90 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    dnase free - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    86
    Roche dnase free enzyme
    Summary of nucleic acids extraction from E. siliculosus brown alga. High yields of good quality DNA and RNA are isolated from as little as 25 Steps 1–5: Harvested tissue is immediately homogenised using commercial 3 mm solid-glass beads in the presence of 1 mL EB containing 100 mM Tris-HCl, 150 mM NaCl, 5 mM DTT and 1% sarkosyl. These stages allow the lysis of the cell wall, the release of highest amount of nucleic acids, the inactivation of cellular nucleases, and the removal of most of the polysaccharides and other insoluble material. Steps 6–10: Simultaneous presence of absolute ethanol and potassium acetate aids polysaccharide precipitation. Moreover proteins, lipids, pigments and cell debris are removed through extraction of the aqueous phase with chloroform. Steps 11–12: Nucleic acids are then recovered by precipitation with 0.8 V of isopropanol and 0.1 V of 3 M sodium acetate (pH 5.2) in the presence of 1% 2-mercaptoetanol at −80°C. During the precipitation step, salts and other solutes are separated from nucleic acids that form a white precipitate collected by centrifugation. The excess of isopropanol and 2-mercaptoetanol are removed through washing the pellet with 75% ethanol. Step 13: All traces of ethanol are removed, the nucleic acid pellet is dried and resuspended in nuclease-free water. After <t>RNase</t> or <t>DNase</t> treatment the superfluous quantities of proteins, polysaccharides, lipids, and cell debris were removed from the extracted DNA and RNA through double extended purification treatment with phenol:chloroform:isoamyl alcohol.
    Dnase Free Enzyme, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free enzyme/product/Roche
    Average 86 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    dnase free enzyme - by Bioz Stars, 2020-08
    86/100 stars
      Buy from Supplier

    88
    Roche dnase free rnaase
    Summary of nucleic acids extraction from E. siliculosus brown alga. High yields of good quality DNA and RNA are isolated from as little as 25 Steps 1–5: Harvested tissue is immediately homogenised using commercial 3 mm solid-glass beads in the presence of 1 mL EB containing 100 mM Tris-HCl, 150 mM NaCl, 5 mM DTT and 1% sarkosyl. These stages allow the lysis of the cell wall, the release of highest amount of nucleic acids, the inactivation of cellular nucleases, and the removal of most of the polysaccharides and other insoluble material. Steps 6–10: Simultaneous presence of absolute ethanol and potassium acetate aids polysaccharide precipitation. Moreover proteins, lipids, pigments and cell debris are removed through extraction of the aqueous phase with chloroform. Steps 11–12: Nucleic acids are then recovered by precipitation with 0.8 V of isopropanol and 0.1 V of 3 M sodium acetate (pH 5.2) in the presence of 1% 2-mercaptoetanol at −80°C. During the precipitation step, salts and other solutes are separated from nucleic acids that form a white precipitate collected by centrifugation. The excess of isopropanol and 2-mercaptoetanol are removed through washing the pellet with 75% ethanol. Step 13: All traces of ethanol are removed, the nucleic acid pellet is dried and resuspended in nuclease-free water. After <t>RNase</t> or <t>DNase</t> treatment the superfluous quantities of proteins, polysaccharides, lipids, and cell debris were removed from the extracted DNA and RNA through double extended purification treatment with phenol:chloroform:isoamyl alcohol.
    Dnase Free Rnaase, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free rnaase/product/Roche
    Average 88 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    dnase free rnaase - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    90
    TransGen biotech co dnase free rnasea
    Summary of nucleic acids extraction from E. siliculosus brown alga. High yields of good quality DNA and RNA are isolated from as little as 25 Steps 1–5: Harvested tissue is immediately homogenised using commercial 3 mm solid-glass beads in the presence of 1 mL EB containing 100 mM Tris-HCl, 150 mM NaCl, 5 mM DTT and 1% sarkosyl. These stages allow the lysis of the cell wall, the release of highest amount of nucleic acids, the inactivation of cellular nucleases, and the removal of most of the polysaccharides and other insoluble material. Steps 6–10: Simultaneous presence of absolute ethanol and potassium acetate aids polysaccharide precipitation. Moreover proteins, lipids, pigments and cell debris are removed through extraction of the aqueous phase with chloroform. Steps 11–12: Nucleic acids are then recovered by precipitation with 0.8 V of isopropanol and 0.1 V of 3 M sodium acetate (pH 5.2) in the presence of 1% 2-mercaptoetanol at −80°C. During the precipitation step, salts and other solutes are separated from nucleic acids that form a white precipitate collected by centrifugation. The excess of isopropanol and 2-mercaptoetanol are removed through washing the pellet with 75% ethanol. Step 13: All traces of ethanol are removed, the nucleic acid pellet is dried and resuspended in nuclease-free water. After <t>RNase</t> or <t>DNase</t> treatment the superfluous quantities of proteins, polysaccharides, lipids, and cell debris were removed from the extracted DNA and RNA through double extended purification treatment with phenol:chloroform:isoamyl alcohol.
    Dnase Free Rnasea, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase free rnasea/product/TransGen biotech co
    Average 90 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    dnase free rnasea - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    DAPI-positivity of tau deposits is not affected by RNase A and/or DNase I treatments. (A and E) DNase I (–)/RNase A (–); (B and F) DNase I (+)/RNase A (–); (C and G) DNase I (–)/RNase A (+); (D and H) DNase I (+)/RNase A (+). (A–D) All sections were double-stained with ThS (green) and DAPI (blue). (E–H) All sections were stained with DAPI (blue) alone. Calibration bars = 10 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; ThS, thioflavin-S.

    Journal: Journal of Histochemistry and Cytochemistry

    Article Title: 4′,6-Diamidino-2-Phenylindole Distinctly Labels Tau Deposits

    doi: 10.1369/0022155418793600

    Figure Lengend Snippet: DAPI-positivity of tau deposits is not affected by RNase A and/or DNase I treatments. (A and E) DNase I (–)/RNase A (–); (B and F) DNase I (+)/RNase A (–); (C and G) DNase I (–)/RNase A (+); (D and H) DNase I (+)/RNase A (+). (A–D) All sections were double-stained with ThS (green) and DAPI (blue). (E–H) All sections were stained with DAPI (blue) alone. Calibration bars = 10 µm. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; ThS, thioflavin-S.

    Article Snippet: To investigate the nuclease effect, the deparaffinized sections were incubated in RNase-free DNase I solution (400 U/ml, Sigma-Aldrich), DNase-free RNase A solution (200 μg/ml, Sigma-Aldrich), or a combination of DNase I and RNase A for 3.5 hr, and the efficacy of nucleic acid elimination was validated by staining with 2 μg/ml DAPI and 10 μg/ml ethidium bromide (EtBr; Nippon Gene, Tokyo, Japan).

    Techniques: Staining

    In vivo interaction of Hrp59 and Hrp65. (A) RNA-mediated association of Hrp59 and Hrp65. Protein complexes were immunoprecipitated from native nuclear extracts of C. tentans tissue culture cells using mAb 4E9 against Hrp65. Mock IPs were performed in parallel in the absence of primary antibody (lanes 4 and 8). The immunoprecipitated material bound to Sepharose was incubated with or without RNase A and washed in order to remove RNase-released material. Proteins still bound after RNase digestion were analyzed by SDS-PAGE and Western blotting with antibodies against either Hrp59 (lanes 1–4) or Hrp65 (lanes 5–8). Nuclear extract was loaded as input (lanes 1 and 5). (B) Native coIP followed by DNase I digestion. The experiment was performed as in A and the bound proteins were incubated with (lane 3) or without (lane 2) DNase I and washed in order to remove DNase-released material. Nuclear extract was loaded as input (lane 1). (C) Direct interaction of Hrp59 and Hrp65: coimmunoprecipitation after in vivo cross-linking. C. tentans tissue culture cells were treated with (lanes 2 and 5) or without (lanes 3 and 6) DSP. Nuclear extracts were prepared in 8 M urea and used for IP with mAb 4E9 against hrp65 (lanes 2 and 3, and 5 and 6). Bound proteins were analyzed by SDS-PAGE and Western blotting with antibodies against either Hrp59 (lanes 1–3) or U2B′′ as a negative control (lanes 4–6). Nuclear extract from DSP-treated cells was loaded as input (lanes 1 and 4). HC and LC indicate cross-reactivity of the secondary antibodies used for Western blotting with the heavy and light chains, respectively, of the rabbit anti–mouse immunoglobulin used for IP.

    Journal: The Journal of Cell Biology

    Article Title: Hrp59, an hnRNP M protein in Chironomus and Drosophila, binds to exonic splicing enhancers and is required for expression of a subset of mRNAs

    doi: 10.1083/jcb.200407173

    Figure Lengend Snippet: In vivo interaction of Hrp59 and Hrp65. (A) RNA-mediated association of Hrp59 and Hrp65. Protein complexes were immunoprecipitated from native nuclear extracts of C. tentans tissue culture cells using mAb 4E9 against Hrp65. Mock IPs were performed in parallel in the absence of primary antibody (lanes 4 and 8). The immunoprecipitated material bound to Sepharose was incubated with or without RNase A and washed in order to remove RNase-released material. Proteins still bound after RNase digestion were analyzed by SDS-PAGE and Western blotting with antibodies against either Hrp59 (lanes 1–4) or Hrp65 (lanes 5–8). Nuclear extract was loaded as input (lanes 1 and 5). (B) Native coIP followed by DNase I digestion. The experiment was performed as in A and the bound proteins were incubated with (lane 3) or without (lane 2) DNase I and washed in order to remove DNase-released material. Nuclear extract was loaded as input (lane 1). (C) Direct interaction of Hrp59 and Hrp65: coimmunoprecipitation after in vivo cross-linking. C. tentans tissue culture cells were treated with (lanes 2 and 5) or without (lanes 3 and 6) DSP. Nuclear extracts were prepared in 8 M urea and used for IP with mAb 4E9 against hrp65 (lanes 2 and 3, and 5 and 6). Bound proteins were analyzed by SDS-PAGE and Western blotting with antibodies against either Hrp59 (lanes 1–3) or U2B′′ as a negative control (lanes 4–6). Nuclear extract from DSP-treated cells was loaded as input (lanes 1 and 4). HC and LC indicate cross-reactivity of the secondary antibodies used for Western blotting with the heavy and light chains, respectively, of the rabbit anti–mouse immunoglobulin used for IP.

    Article Snippet: RNase treatment of C. tentans salivary glands Salivary glands were dissected and preextracted as for BrUTP incorporation, washed in TBS and incubated for 30 min with 0.1 mg/ml DNase-free RNase A (Sigma-Aldrich) in TBS, or with TBS alone.

    Techniques: In Vivo, Immunoprecipitation, Incubation, SDS Page, Western Blot, Co-Immunoprecipitation Assay, Negative Control

    Summary of nucleic acids extraction from E. siliculosus brown alga. High yields of good quality DNA and RNA are isolated from as little as 25 Steps 1–5: Harvested tissue is immediately homogenised using commercial 3 mm solid-glass beads in the presence of 1 mL EB containing 100 mM Tris-HCl, 150 mM NaCl, 5 mM DTT and 1% sarkosyl. These stages allow the lysis of the cell wall, the release of highest amount of nucleic acids, the inactivation of cellular nucleases, and the removal of most of the polysaccharides and other insoluble material. Steps 6–10: Simultaneous presence of absolute ethanol and potassium acetate aids polysaccharide precipitation. Moreover proteins, lipids, pigments and cell debris are removed through extraction of the aqueous phase with chloroform. Steps 11–12: Nucleic acids are then recovered by precipitation with 0.8 V of isopropanol and 0.1 V of 3 M sodium acetate (pH 5.2) in the presence of 1% 2-mercaptoetanol at −80°C. During the precipitation step, salts and other solutes are separated from nucleic acids that form a white precipitate collected by centrifugation. The excess of isopropanol and 2-mercaptoetanol are removed through washing the pellet with 75% ethanol. Step 13: All traces of ethanol are removed, the nucleic acid pellet is dried and resuspended in nuclease-free water. After RNase or DNase treatment the superfluous quantities of proteins, polysaccharides, lipids, and cell debris were removed from the extracted DNA and RNA through double extended purification treatment with phenol:chloroform:isoamyl alcohol.

    Journal: PLoS ONE

    Article Title: A Simple and Effective Method for High Quality Co-Extraction of Genomic DNA and Total RNA from Low Biomass Ectocarpus siliculosus, the Model Brown Alga

    doi: 10.1371/journal.pone.0096470

    Figure Lengend Snippet: Summary of nucleic acids extraction from E. siliculosus brown alga. High yields of good quality DNA and RNA are isolated from as little as 25 Steps 1–5: Harvested tissue is immediately homogenised using commercial 3 mm solid-glass beads in the presence of 1 mL EB containing 100 mM Tris-HCl, 150 mM NaCl, 5 mM DTT and 1% sarkosyl. These stages allow the lysis of the cell wall, the release of highest amount of nucleic acids, the inactivation of cellular nucleases, and the removal of most of the polysaccharides and other insoluble material. Steps 6–10: Simultaneous presence of absolute ethanol and potassium acetate aids polysaccharide precipitation. Moreover proteins, lipids, pigments and cell debris are removed through extraction of the aqueous phase with chloroform. Steps 11–12: Nucleic acids are then recovered by precipitation with 0.8 V of isopropanol and 0.1 V of 3 M sodium acetate (pH 5.2) in the presence of 1% 2-mercaptoetanol at −80°C. During the precipitation step, salts and other solutes are separated from nucleic acids that form a white precipitate collected by centrifugation. The excess of isopropanol and 2-mercaptoetanol are removed through washing the pellet with 75% ethanol. Step 13: All traces of ethanol are removed, the nucleic acid pellet is dried and resuspended in nuclease-free water. After RNase or DNase treatment the superfluous quantities of proteins, polysaccharides, lipids, and cell debris were removed from the extracted DNA and RNA through double extended purification treatment with phenol:chloroform:isoamyl alcohol.

    Article Snippet: (a) RNase or DNase treatment To obtain pure DNA, treat aliquots (10–25 µg) of nucleic acid mixtures with 1 µL of RNase A, DNase free enzyme (0.1 mg ml−1 ) (Roche Diagnostic Mannheim, Germany) in a final volume of 100 µL, for 20 min at 37°C.

    Techniques: Isolation, Lysis, Centrifugation, Purification