dna tiling microarray Search Results


genomic dna  (NimbleGen Systems GmbH)
90
NimbleGen Systems GmbH genomic dna
Analysis of the CDX2 promoter . A) The promoter region cloned in pGL4.10. The coordinates are relative to +1, the transcriptional start site. The arrows indicate primers used in the real-time qPCR. The possible HNF4α binding site and the mutated sequence is shown below. B) HNF4α <t>ChIP-chip</t> and AcHis3 ChIP-chip results for probes spanning the CDX2 promoter. Enrichments are shown as Log2 ratios between immunoprecipitated and input <t>DNA.</t> N = 3. C) Real-time qPCR with CDX2 and IgG intron primers using HNF4α ChIP, HA ChIP and input DNA. Enrichments are presented as percent of total input. N = 4. The CDX2 promoter enrichment is statistical significant (p-values < 0.05, Student T test). D) Gel shift analysis of the HNF4α site in the CDX2 promoter using Caco-2 nuclear extract (lane 1). Competition by unlabelled wt (lane 2), mut-HNF4α oligonucleotides (lane 3) and an unrelated oligonucleotide (lane 4) demonstrating a specific binding. One of the two protein/DNA bands can be supershifted with HNF4α antibody (lane 5). E) Promoter analysis of CDX2 promoter in Caco-2 (grey bars) and COS7 (white bars) cells with and without co-transfection of HNF4α expression plasmid. The binding site for HNF4α was mutated in order to analyze the functional importance of the HNF4α site (pGL4-CDX2 mutHNF4). The luciferase activity was corrected for transfection efficiency and normalized to the expression of pGL4-CDX2, N = 4.
Genomic Dna, supplied by NimbleGen Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genomic dna/product/NimbleGen Systems GmbH
Average 90 stars, based on 1 article reviews
genomic dna - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
dna tiling microarrays covering 5 kb around the transcription start site of 25,000 mouse promoters  (NimbleGen Systems GmbH)
90
NimbleGen Systems GmbH dna tiling microarrays covering 5 kb around the transcription start site of 25,000 mouse promoters
Analysis of the CDX2 promoter . A) The promoter region cloned in pGL4.10. The coordinates are relative to +1, the transcriptional start site. The arrows indicate primers used in the real-time qPCR. The possible HNF4α binding site and the mutated sequence is shown below. B) HNF4α <t>ChIP-chip</t> and AcHis3 ChIP-chip results for probes spanning the CDX2 promoter. Enrichments are shown as Log2 ratios between immunoprecipitated and input <t>DNA.</t> N = 3. C) Real-time qPCR with CDX2 and IgG intron primers using HNF4α ChIP, HA ChIP and input DNA. Enrichments are presented as percent of total input. N = 4. The CDX2 promoter enrichment is statistical significant (p-values < 0.05, Student T test). D) Gel shift analysis of the HNF4α site in the CDX2 promoter using Caco-2 nuclear extract (lane 1). Competition by unlabelled wt (lane 2), mut-HNF4α oligonucleotides (lane 3) and an unrelated oligonucleotide (lane 4) demonstrating a specific binding. One of the two protein/DNA bands can be supershifted with HNF4α antibody (lane 5). E) Promoter analysis of CDX2 promoter in Caco-2 (grey bars) and COS7 (white bars) cells with and without co-transfection of HNF4α expression plasmid. The binding site for HNF4α was mutated in order to analyze the functional importance of the HNF4α site (pGL4-CDX2 mutHNF4). The luciferase activity was corrected for transfection efficiency and normalized to the expression of pGL4-CDX2, N = 4.
Dna Tiling Microarrays Covering 5 Kb Around The Transcription Start Site Of 25,000 Mouse Promoters, supplied by NimbleGen Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna tiling microarrays covering 5 kb around the transcription start site of 25,000 mouse promoters/product/NimbleGen Systems GmbH
Average 90 stars, based on 1 article reviews
dna tiling microarrays covering 5 kb around the transcription start site of 25,000 mouse promoters - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
a tiling resolution dna microarray with complete coverage of the human genome  (Snijders Scientific)
90
Snijders Scientific a tiling resolution dna microarray with complete coverage of the human genome
Analysis of the CDX2 promoter . A) The promoter region cloned in pGL4.10. The coordinates are relative to +1, the transcriptional start site. The arrows indicate primers used in the real-time qPCR. The possible HNF4α binding site and the mutated sequence is shown below. B) HNF4α <t>ChIP-chip</t> and AcHis3 ChIP-chip results for probes spanning the CDX2 promoter. Enrichments are shown as Log2 ratios between immunoprecipitated and input <t>DNA.</t> N = 3. C) Real-time qPCR with CDX2 and IgG intron primers using HNF4α ChIP, HA ChIP and input DNA. Enrichments are presented as percent of total input. N = 4. The CDX2 promoter enrichment is statistical significant (p-values < 0.05, Student T test). D) Gel shift analysis of the HNF4α site in the CDX2 promoter using Caco-2 nuclear extract (lane 1). Competition by unlabelled wt (lane 2), mut-HNF4α oligonucleotides (lane 3) and an unrelated oligonucleotide (lane 4) demonstrating a specific binding. One of the two protein/DNA bands can be supershifted with HNF4α antibody (lane 5). E) Promoter analysis of CDX2 promoter in Caco-2 (grey bars) and COS7 (white bars) cells with and without co-transfection of HNF4α expression plasmid. The binding site for HNF4α was mutated in order to analyze the functional importance of the HNF4α site (pGL4-CDX2 mutHNF4). The luciferase activity was corrected for transfection efficiency and normalized to the expression of pGL4-CDX2, N = 4.
A Tiling Resolution Dna Microarray With Complete Coverage Of The Human Genome, supplied by Snijders Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a tiling resolution dna microarray with complete coverage of the human genome/product/Snijders Scientific
Average 90 stars, based on 1 article reviews
a tiling resolution dna microarray with complete coverage of the human genome - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
c. albicans whole-genome tiled oligonucleotide dna microarray  (NimbleGen Systems GmbH)
90
NimbleGen Systems GmbH c. albicans whole-genome tiled oligonucleotide dna microarray
Analysis of the CDX2 promoter . A) The promoter region cloned in pGL4.10. The coordinates are relative to +1, the transcriptional start site. The arrows indicate primers used in the real-time qPCR. The possible HNF4α binding site and the mutated sequence is shown below. B) HNF4α <t>ChIP-chip</t> and AcHis3 ChIP-chip results for probes spanning the CDX2 promoter. Enrichments are shown as Log2 ratios between immunoprecipitated and input <t>DNA.</t> N = 3. C) Real-time qPCR with CDX2 and IgG intron primers using HNF4α ChIP, HA ChIP and input DNA. Enrichments are presented as percent of total input. N = 4. The CDX2 promoter enrichment is statistical significant (p-values < 0.05, Student T test). D) Gel shift analysis of the HNF4α site in the CDX2 promoter using Caco-2 nuclear extract (lane 1). Competition by unlabelled wt (lane 2), mut-HNF4α oligonucleotides (lane 3) and an unrelated oligonucleotide (lane 4) demonstrating a specific binding. One of the two protein/DNA bands can be supershifted with HNF4α antibody (lane 5). E) Promoter analysis of CDX2 promoter in Caco-2 (grey bars) and COS7 (white bars) cells with and without co-transfection of HNF4α expression plasmid. The binding site for HNF4α was mutated in order to analyze the functional importance of the HNF4α site (pGL4-CDX2 mutHNF4). The luciferase activity was corrected for transfection efficiency and normalized to the expression of pGL4-CDX2, N = 4.
C. Albicans Whole Genome Tiled Oligonucleotide Dna Microarray, supplied by NimbleGen Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c. albicans whole-genome tiled oligonucleotide dna microarray/product/NimbleGen Systems GmbH
Average 90 stars, based on 1 article reviews
c. albicans whole-genome tiled oligonucleotide dna microarray - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Analysis of the CDX2 promoter . A) The promoter region cloned in pGL4.10. The coordinates are relative to +1, the transcriptional start site. The arrows indicate primers used in the real-time qPCR. The possible HNF4α binding site and the mutated sequence is shown below. B) HNF4α ChIP-chip and AcHis3 ChIP-chip results for probes spanning the CDX2 promoter. Enrichments are shown as Log2 ratios between immunoprecipitated and input DNA. N = 3. C) Real-time qPCR with CDX2 and IgG intron primers using HNF4α ChIP, HA ChIP and input DNA. Enrichments are presented as percent of total input. N = 4. The CDX2 promoter enrichment is statistical significant (p-values < 0.05, Student T test). D) Gel shift analysis of the HNF4α site in the CDX2 promoter using Caco-2 nuclear extract (lane 1). Competition by unlabelled wt (lane 2), mut-HNF4α oligonucleotides (lane 3) and an unrelated oligonucleotide (lane 4) demonstrating a specific binding. One of the two protein/DNA bands can be supershifted with HNF4α antibody (lane 5). E) Promoter analysis of CDX2 promoter in Caco-2 (grey bars) and COS7 (white bars) cells with and without co-transfection of HNF4α expression plasmid. The binding site for HNF4α was mutated in order to analyze the functional importance of the HNF4α site (pGL4-CDX2 mutHNF4). The luciferase activity was corrected for transfection efficiency and normalized to the expression of pGL4-CDX2, N = 4.

Journal: BMC Gastroenterology

Article Title: Mapping of HNF4α target genes in intestinal epithelial cells

doi: 10.1186/1471-230X-9-68

Figure Lengend Snippet: Analysis of the CDX2 promoter . A) The promoter region cloned in pGL4.10. The coordinates are relative to +1, the transcriptional start site. The arrows indicate primers used in the real-time qPCR. The possible HNF4α binding site and the mutated sequence is shown below. B) HNF4α ChIP-chip and AcHis3 ChIP-chip results for probes spanning the CDX2 promoter. Enrichments are shown as Log2 ratios between immunoprecipitated and input DNA. N = 3. C) Real-time qPCR with CDX2 and IgG intron primers using HNF4α ChIP, HA ChIP and input DNA. Enrichments are presented as percent of total input. N = 4. The CDX2 promoter enrichment is statistical significant (p-values < 0.05, Student T test). D) Gel shift analysis of the HNF4α site in the CDX2 promoter using Caco-2 nuclear extract (lane 1). Competition by unlabelled wt (lane 2), mut-HNF4α oligonucleotides (lane 3) and an unrelated oligonucleotide (lane 4) demonstrating a specific binding. One of the two protein/DNA bands can be supershifted with HNF4α antibody (lane 5). E) Promoter analysis of CDX2 promoter in Caco-2 (grey bars) and COS7 (white bars) cells with and without co-transfection of HNF4α expression plasmid. The binding site for HNF4α was mutated in order to analyze the functional importance of the HNF4α site (pGL4-CDX2 mutHNF4). The luciferase activity was corrected for transfection efficiency and normalized to the expression of pGL4-CDX2, N = 4.

Article Snippet: The amplified ChIP DNA was sent to NimbleGen Systems Inc. for labeling and hybridization to a 1.5 kb promoter array containing probes covering the region from -1350 to +150 of 24,275 human genes. (See http://www.nimblegen.com/products/chip/index.html for a description of the promoter array).

Techniques: Clone Assay, Binding Assay, Sequencing, ChIP-chip, Immunoprecipitation, Gel Shift, Cotransfection, Expressing, Plasmid Preparation, Functional Assay, Luciferase, Activity Assay, Transfection

Analysis of the trehalase ( TREH ) promoter . A) Map of the TREH promoter region. PCR primers, the HNF4α binding site, and the mutations are shown. B) HNF4α ChIP-chip and AcHis3 ChIP-chip results for probes spanning the TREH promoter. N = 3. C) Real-time qPCR analysis of HNF4α ChIP, HA ChIP and input DNA using TREH and IgG intron primers. N = 3. The TREH promoter enrichment is statistical significant (p-values < 0.05, Student T test). D) Gel shift analysis of the HNF4α site in the TREH promoter. E) Promoter analysis of the TREH promoter in Caco-2 (grey bars) and COS7 (white bars) cells with and without co-transfection of HNF4α expression plasmid and a mutation in the HNF4α site (pGL4-TREH mutHNF4). The luciferase activity was corrected for transfection efficiency and normalized to the expression of pGL4-TREH, N = 4.

Journal: BMC Gastroenterology

Article Title: Mapping of HNF4α target genes in intestinal epithelial cells

doi: 10.1186/1471-230X-9-68

Figure Lengend Snippet: Analysis of the trehalase ( TREH ) promoter . A) Map of the TREH promoter region. PCR primers, the HNF4α binding site, and the mutations are shown. B) HNF4α ChIP-chip and AcHis3 ChIP-chip results for probes spanning the TREH promoter. N = 3. C) Real-time qPCR analysis of HNF4α ChIP, HA ChIP and input DNA using TREH and IgG intron primers. N = 3. The TREH promoter enrichment is statistical significant (p-values < 0.05, Student T test). D) Gel shift analysis of the HNF4α site in the TREH promoter. E) Promoter analysis of the TREH promoter in Caco-2 (grey bars) and COS7 (white bars) cells with and without co-transfection of HNF4α expression plasmid and a mutation in the HNF4α site (pGL4-TREH mutHNF4). The luciferase activity was corrected for transfection efficiency and normalized to the expression of pGL4-TREH, N = 4.

Article Snippet: The amplified ChIP DNA was sent to NimbleGen Systems Inc. for labeling and hybridization to a 1.5 kb promoter array containing probes covering the region from -1350 to +150 of 24,275 human genes. (See http://www.nimblegen.com/products/chip/index.html for a description of the promoter array).

Techniques: Binding Assay, ChIP-chip, Gel Shift, Cotransfection, Expressing, Plasmid Preparation, Mutagenesis, Luciferase, Activity Assay, Transfection

Analysis of the cingulin ( CGN ) promoter . A) Map of the CGN promoter region. PCR primers, the HNF4α binding site, and the mutations are shown. B) HNF4α ChIP-chip and AcHis3 ChIP-chip results for probes spanning the CGN promoter. N = 3. C) Real-time qPCR analysis of HNF4α ChIP, HA ChIP and input DNA using CGN and IgG intron primers. N = 3. The CGN promoter enrichment is statistical significant (p-values < 0.05, Student T test). D) Gel shift analysis of the HNF4α site in the CGN promoter. E) Promoter analysis of the CGN promoter in Caco-2 (grey bars) and COS7 (white bars) cells with and without co-transfection of HNF4α expression plasmid and a mutation in the HNF4α site (pGL4-CGN mutHNF4). The luciferase activity was corrected for transfection efficiency and normalized to the expression of pGL4-CGN, N = 4.

Journal: BMC Gastroenterology

Article Title: Mapping of HNF4α target genes in intestinal epithelial cells

doi: 10.1186/1471-230X-9-68

Figure Lengend Snippet: Analysis of the cingulin ( CGN ) promoter . A) Map of the CGN promoter region. PCR primers, the HNF4α binding site, and the mutations are shown. B) HNF4α ChIP-chip and AcHis3 ChIP-chip results for probes spanning the CGN promoter. N = 3. C) Real-time qPCR analysis of HNF4α ChIP, HA ChIP and input DNA using CGN and IgG intron primers. N = 3. The CGN promoter enrichment is statistical significant (p-values < 0.05, Student T test). D) Gel shift analysis of the HNF4α site in the CGN promoter. E) Promoter analysis of the CGN promoter in Caco-2 (grey bars) and COS7 (white bars) cells with and without co-transfection of HNF4α expression plasmid and a mutation in the HNF4α site (pGL4-CGN mutHNF4). The luciferase activity was corrected for transfection efficiency and normalized to the expression of pGL4-CGN, N = 4.

Article Snippet: The amplified ChIP DNA was sent to NimbleGen Systems Inc. for labeling and hybridization to a 1.5 kb promoter array containing probes covering the region from -1350 to +150 of 24,275 human genes. (See http://www.nimblegen.com/products/chip/index.html for a description of the promoter array).

Techniques: Binding Assay, ChIP-chip, Gel Shift, Cotransfection, Expressing, Plasmid Preparation, Mutagenesis, Luciferase, Activity Assay, Transfection

HNF4α ChIP on mouse small intestinal epithelium . Chromatin immunoprecipitation analysis of HNF4α binding to HNF1α ( Tcf1 ), phosphoenolpyruvate carboxykinase 1 ( Pck1 ), apolipoprotein C3 ( Apoc3 ), Cdx-2 ( Cdx2 ), trehalase ( Treh ), and cingulin ( Cgn ) promoters in mouse small intestinal epithelium. An intron in the IgG gene served as a negative control (IgG). The analyzed promoter regions are all conserved between human and mouse. Enrichments are represented as percent of the total amount of genomic input DNA in ChIP. Significant enrichments are indicated (p-values < 0.001 is shown by ***, p-values < 0.05 is shown by *).

Journal: BMC Gastroenterology

Article Title: Mapping of HNF4α target genes in intestinal epithelial cells

doi: 10.1186/1471-230X-9-68

Figure Lengend Snippet: HNF4α ChIP on mouse small intestinal epithelium . Chromatin immunoprecipitation analysis of HNF4α binding to HNF1α ( Tcf1 ), phosphoenolpyruvate carboxykinase 1 ( Pck1 ), apolipoprotein C3 ( Apoc3 ), Cdx-2 ( Cdx2 ), trehalase ( Treh ), and cingulin ( Cgn ) promoters in mouse small intestinal epithelium. An intron in the IgG gene served as a negative control (IgG). The analyzed promoter regions are all conserved between human and mouse. Enrichments are represented as percent of the total amount of genomic input DNA in ChIP. Significant enrichments are indicated (p-values < 0.001 is shown by ***, p-values < 0.05 is shown by *).

Article Snippet: The amplified ChIP DNA was sent to NimbleGen Systems Inc. for labeling and hybridization to a 1.5 kb promoter array containing probes covering the region from -1350 to +150 of 24,275 human genes. (See http://www.nimblegen.com/products/chip/index.html for a description of the promoter array).

Techniques: Chromatin Immunoprecipitation, Binding Assay, Negative Control