dna quantification Search Results


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  • 94
    Millipore dna quantification fluorescence assay kit
    Dna Quantification Fluorescence Assay Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna quantification fluorescence assay kit/product/Millipore
    Average 94 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
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    99
    Millipore dna quantification
    Decellularization steps: ( a ) Decellularization of the pancreatic body tail scaffold. Images illustrate the gradual change in color caused by perfusion-decellularization in mouse pancreases. Treatment with 1% triton x-100 resulted in a <t>decellularized</t> pancreas after 315 min. b H E staining showing that there were no remnant cells after the completion of decellularization. c <t>DNA</t> quantification demonstrating that the amount of DNA was clearly lower in the treated organs than in natural organs (from 931.9 ± 267.8 to 11.7 ± 3.6 ng/mg), P
    Dna Quantification, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna quantification/product/Millipore
    Average 99 stars, based on 134 article reviews
    Price from $9.99 to $1999.99
    dna quantification - by Bioz Stars, 2020-08
    99/100 stars
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    99
    Millipore dna quantification assay
    (A) Scaffold degradation. PLGA scaffolds containing BMSCs cultured in static conditions in osteogenic vs. control media as indicated were measured every 7th day of the experiment. Data presented are medians, error bars represent minimal and maximal data points, n = 6 scaffolds. (B,C) <t>DNA</t> quantification. BMSCs on PLGA scaffolds were cultured in static and perfusion conditions in osteogenic vs. control media as indicated. Samples taken after 7 and 21 days were analyzed using <t>Hoechst.</t> Data presented are means ± SD, n = 3 technical replicates per donor and experimental group. * P
    Dna Quantification Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna quantification assay/product/Millipore
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    dna quantification assay - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    96
    Millipore dna quantification kit
    <t>DNA</t> content ( A ) <t>ALP</t> activity ( B ) and calcium content ( C ) of human marrow stromal cells (hMSCs) seeded on CAP treated pNF, GLU-pNF, and ASP-pNF and incubated in osteogenic medium for up to 28 days. Error bars represent mean ± SE (n = 5) [significant differences were determined by one-way ANOVA [Newman–Keuls multiple comparison test, (*p\0.05, **p\0.01, ***p\0.001)].
    Dna Quantification Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna quantification kit/product/Millipore
    Average 96 stars, based on 101 article reviews
    Price from $9.99 to $1999.99
    dna quantification kit - by Bioz Stars, 2020-08
    96/100 stars
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    88
    Thermo Fisher picogreen dna quantification assay
    In vitro studies on the engineered fibrous PLGA, gelatin and GelMA-10 scaffolds. Representative live/dead images based on viability test (A), quantification of cell viability (B), and cell proliferation on the electrospun scaffolds measured using <t>PicoGreen®</t> <t>DNA</t> quantification kit (C). Green fluorescent cells are living cells and red fluorescent cells indicate dead cells (scale bar = 100 μm). Most cells were alive when cultured on all scaffolds and showed a time-dependent increase in cell number. **significant difference between GelMA-10 and PLGA scaffolds. *significant difference between GelMA-10 and gelatin scaffolds. These results indicate that all PLGA, gelatin and GelMA-10 scaffolds have good cyto-compatibility. In particular, GelMA-10 scaffolds supported highest cell proliferation.
    Picogreen Dna Quantification Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/picogreen dna quantification assay/product/Thermo Fisher
    Average 88 stars, based on 98 article reviews
    Price from $9.99 to $1999.99
    picogreen dna quantification assay - by Bioz Stars, 2020-08
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    90
    Keygen Biotech dna content quantitation assay
    In vitro studies on the engineered fibrous PLGA, gelatin and GelMA-10 scaffolds. Representative live/dead images based on viability test (A), quantification of cell viability (B), and cell proliferation on the electrospun scaffolds measured using <t>PicoGreen®</t> <t>DNA</t> quantification kit (C). Green fluorescent cells are living cells and red fluorescent cells indicate dead cells (scale bar = 100 μm). Most cells were alive when cultured on all scaffolds and showed a time-dependent increase in cell number. **significant difference between GelMA-10 and PLGA scaffolds. *significant difference between GelMA-10 and gelatin scaffolds. These results indicate that all PLGA, gelatin and GelMA-10 scaffolds have good cyto-compatibility. In particular, GelMA-10 scaffolds supported highest cell proliferation.
    Dna Content Quantitation Assay, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna content quantitation assay/product/Keygen Biotech
    Average 90 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    dna content quantitation assay - by Bioz Stars, 2020-08
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    86
    Thermo Fisher qubit dna quantitation assay
    In vitro studies on the engineered fibrous PLGA, gelatin and GelMA-10 scaffolds. Representative live/dead images based on viability test (A), quantification of cell viability (B), and cell proliferation on the electrospun scaffolds measured using <t>PicoGreen®</t> <t>DNA</t> quantification kit (C). Green fluorescent cells are living cells and red fluorescent cells indicate dead cells (scale bar = 100 μm). Most cells were alive when cultured on all scaffolds and showed a time-dependent increase in cell number. **significant difference between GelMA-10 and PLGA scaffolds. *significant difference between GelMA-10 and gelatin scaffolds. These results indicate that all PLGA, gelatin and GelMA-10 scaffolds have good cyto-compatibility. In particular, GelMA-10 scaffolds supported highest cell proliferation.
    Qubit Dna Quantitation Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qubit dna quantitation assay/product/Thermo Fisher
    Average 86 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    qubit dna quantitation assay - by Bioz Stars, 2020-08
    86/100 stars
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    90
    Keygen Biotech dna content quantitation assay kit
    In vitro studies on the engineered fibrous PLGA, gelatin and GelMA-10 scaffolds. Representative live/dead images based on viability test (A), quantification of cell viability (B), and cell proliferation on the electrospun scaffolds measured using <t>PicoGreen®</t> <t>DNA</t> quantification kit (C). Green fluorescent cells are living cells and red fluorescent cells indicate dead cells (scale bar = 100 μm). Most cells were alive when cultured on all scaffolds and showed a time-dependent increase in cell number. **significant difference between GelMA-10 and PLGA scaffolds. *significant difference between GelMA-10 and gelatin scaffolds. These results indicate that all PLGA, gelatin and GelMA-10 scaffolds have good cyto-compatibility. In particular, GelMA-10 scaffolds supported highest cell proliferation.
    Dna Content Quantitation Assay Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 90/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna content quantitation assay kit/product/Keygen Biotech
    Average 90 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    dna content quantitation assay kit - by Bioz Stars, 2020-08
    90/100 stars
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    88
    Thermo Fisher fluoreporter blue dna quantification assay
    In vitro studies on the engineered fibrous PLGA, gelatin and GelMA-10 scaffolds. Representative live/dead images based on viability test (A), quantification of cell viability (B), and cell proliferation on the electrospun scaffolds measured using <t>PicoGreen®</t> <t>DNA</t> quantification kit (C). Green fluorescent cells are living cells and red fluorescent cells indicate dead cells (scale bar = 100 μm). Most cells were alive when cultured on all scaffolds and showed a time-dependent increase in cell number. **significant difference between GelMA-10 and PLGA scaffolds. *significant difference between GelMA-10 and gelatin scaffolds. These results indicate that all PLGA, gelatin and GelMA-10 scaffolds have good cyto-compatibility. In particular, GelMA-10 scaffolds supported highest cell proliferation.
    Fluoreporter Blue Dna Quantification Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluoreporter blue dna quantification assay/product/Thermo Fisher
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    fluoreporter blue dna quantification assay - by Bioz Stars, 2020-08
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    Image Search Results


    Decellularization steps: ( a ) Decellularization of the pancreatic body tail scaffold. Images illustrate the gradual change in color caused by perfusion-decellularization in mouse pancreases. Treatment with 1% triton x-100 resulted in a decellularized pancreas after 315 min. b H E staining showing that there were no remnant cells after the completion of decellularization. c DNA quantification demonstrating that the amount of DNA was clearly lower in the treated organs than in natural organs (from 931.9 ± 267.8 to 11.7 ± 3.6 ng/mg), P

    Journal: Journal of Biological Engineering

    Article Title: The rat pancreatic body tail as a source of a novel extracellular matrix scaffold for endocrine pancreas bioengineering

    doi: 10.1186/s13036-018-0096-5

    Figure Lengend Snippet: Decellularization steps: ( a ) Decellularization of the pancreatic body tail scaffold. Images illustrate the gradual change in color caused by perfusion-decellularization in mouse pancreases. Treatment with 1% triton x-100 resulted in a decellularized pancreas after 315 min. b H E staining showing that there were no remnant cells after the completion of decellularization. c DNA quantification demonstrating that the amount of DNA was clearly lower in the treated organs than in natural organs (from 931.9 ± 267.8 to 11.7 ± 3.6 ng/mg), P

    Article Snippet: DNA quantification These decellularized and natural pancreas were digested with papain solution at 60 °C for 6 h. Papain (Sigma-Aldrich) was dissolved at a concentration of 400 mg/ml in 0.1 M phosphate buffer (pH 6.0) with 5 mM cysteine hydrochloride (Sigma-Aldrich) and 5 mM EDTA (Sigma-Aldrich).

    Techniques: Staining

    (A) Scaffold degradation. PLGA scaffolds containing BMSCs cultured in static conditions in osteogenic vs. control media as indicated were measured every 7th day of the experiment. Data presented are medians, error bars represent minimal and maximal data points, n = 6 scaffolds. (B,C) DNA quantification. BMSCs on PLGA scaffolds were cultured in static and perfusion conditions in osteogenic vs. control media as indicated. Samples taken after 7 and 21 days were analyzed using Hoechst. Data presented are means ± SD, n = 3 technical replicates per donor and experimental group. * P

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: A Perfusion Culture System for Assessing Bone Marrow Stromal Cell Differentiation on PLGA Scaffolds for Bone Repair

    doi: 10.3389/fbioe.2018.00161

    Figure Lengend Snippet: (A) Scaffold degradation. PLGA scaffolds containing BMSCs cultured in static conditions in osteogenic vs. control media as indicated were measured every 7th day of the experiment. Data presented are medians, error bars represent minimal and maximal data points, n = 6 scaffolds. (B,C) DNA quantification. BMSCs on PLGA scaffolds were cultured in static and perfusion conditions in osteogenic vs. control media as indicated. Samples taken after 7 and 21 days were analyzed using Hoechst. Data presented are means ± SD, n = 3 technical replicates per donor and experimental group. * P

    Article Snippet: DNA quantification DNA was quantified using Hoechst 33258 (Sigma Aldrich®, Switzerland).

    Techniques: Cell Culture

    DNA assay on the scaffolds seeded with BCH and hMSCs in differentiation medium. Significant differences between each cell type at different time points were found for p

    Journal: PLoS ONE

    Article Title: Nanostructured 3D Constructs Based on Chitosan and Chondroitin Sulphate Multilayers for Cartilage Tissue Engineering

    doi: 10.1371/journal.pone.0055451

    Figure Lengend Snippet: DNA assay on the scaffolds seeded with BCH and hMSCs in differentiation medium. Significant differences between each cell type at different time points were found for p

    Article Snippet: DNA quantification Scaffolds seeded with BCH and hMSCs in differentiation medium at 1, 14 and 35 days were washed with PBS and frozen at −80°C before proteinase K (Sigma Aldrich) digestion.

    Techniques:

    DNA content ( A ) ALP activity ( B ) and calcium content ( C ) of human marrow stromal cells (hMSCs) seeded on CAP treated pNF, GLU-pNF, and ASP-pNF and incubated in osteogenic medium for up to 28 days. Error bars represent mean ± SE (n = 5) [significant differences were determined by one-way ANOVA [Newman–Keuls multiple comparison test, (*p\0.05, **p\0.01, ***p\0.001)].

    Journal: Scientific Reports

    Article Title: Aspartic and Glutamic Acid Templated Peptides Conjugation on Plasma Modified Nanofibers for Osteogenic Differentiation of Human Mesenchymal Stem Cells: A Comparative Study

    doi: 10.1038/s41598-018-36109-5

    Figure Lengend Snippet: DNA content ( A ) ALP activity ( B ) and calcium content ( C ) of human marrow stromal cells (hMSCs) seeded on CAP treated pNF, GLU-pNF, and ASP-pNF and incubated in osteogenic medium for up to 28 days. Error bars represent mean ± SE (n = 5) [significant differences were determined by one-way ANOVA [Newman–Keuls multiple comparison test, (*p\0.05, **p\0.01, ***p\0.001)].

    Article Snippet: Double-stranded DNA content, ALP activity and calcium content of the samples were measured with DNA Quantification Kit (Sigma Aldrich, St. Louis, MO, USA), QuantiChrom ALP assay (Bioassay Systems, Hayward, CA, USA) and QuantiChrom Calcium Assay (Bioassay Systems, Hayward, CA, USA), respectively according to manufacturer’s instructions as previously described .

    Techniques: ALP Assay, Activity Assay, Incubation

    In vitro studies on the engineered fibrous PLGA, gelatin and GelMA-10 scaffolds. Representative live/dead images based on viability test (A), quantification of cell viability (B), and cell proliferation on the electrospun scaffolds measured using PicoGreen® DNA quantification kit (C). Green fluorescent cells are living cells and red fluorescent cells indicate dead cells (scale bar = 100 μm). Most cells were alive when cultured on all scaffolds and showed a time-dependent increase in cell number. **significant difference between GelMA-10 and PLGA scaffolds. *significant difference between GelMA-10 and gelatin scaffolds. These results indicate that all PLGA, gelatin and GelMA-10 scaffolds have good cyto-compatibility. In particular, GelMA-10 scaffolds supported highest cell proliferation.

    Journal: Acta biomaterialia

    Article Title: Cell infiltrative hydrogel fibrous scaffolds for accelerated wound healing

    doi: 10.1016/j.actbio.2016.11.017

    Figure Lengend Snippet: In vitro studies on the engineered fibrous PLGA, gelatin and GelMA-10 scaffolds. Representative live/dead images based on viability test (A), quantification of cell viability (B), and cell proliferation on the electrospun scaffolds measured using PicoGreen® DNA quantification kit (C). Green fluorescent cells are living cells and red fluorescent cells indicate dead cells (scale bar = 100 μm). Most cells were alive when cultured on all scaffolds and showed a time-dependent increase in cell number. **significant difference between GelMA-10 and PLGA scaffolds. *significant difference between GelMA-10 and gelatin scaffolds. These results indicate that all PLGA, gelatin and GelMA-10 scaffolds have good cyto-compatibility. In particular, GelMA-10 scaffolds supported highest cell proliferation.

    Article Snippet: Cell proliferation was evaluated using PicoGreen® DNA quantification assay (Life Technologies, NY).

    Techniques: In Vitro, Cell Culture