dna mini isolation kit Search Results


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  • 99
    Thermo Fisher purelink genomic dna isolation kit
    Purelink Genomic Dna Isolation Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 491 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 491 article reviews
    Price from $9.99 to $1999.99
    purelink genomic dna isolation kit - by Bioz Stars, 2020-09
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    99
    Qiagen qiaamp dna isolation kit
    Experimental verification of a 16-compartment digital assay with comparison to the performance of qPCR assays. ( A ) The graph shows the results for the same amount of reference <t>DNA</t> suspended in different elution buffers and quantified with conventional qPCR and with the synergistic PCR algorithm. Tests performed on Applied Biosystems 7500 Fast RT System on the IVD Cytomegalovirus PCR kit (GeneProof) according to the prescription. Elution buffers: (1) water, (2) AE elution buffer <t>QIAamp</t> DNA Mini Kit (Quiagen), (3) MBL5 NucleoMag Blood 200 uL (MACHEREY-NAGEL), (4) MagJET Whole Blood Genomic DNA Kit (Thermo Scientific). The gray line shows the expected distribution of results for Real-Time assay. ( B ) The graph shows the result of 24 runs of the synergistic assay, each on 16 partitions of the amplification mix. We conducted two series of 12 assays on the two elution buffers (1 and 4) that provided the largest difference in the result of the conventional qPCR analysis. The gray line shows the expected distribution of results from the synergistic assay used in the experiment, which should provide 60% precision of assessment. This distribution was also verified using 10,000 Monte-Carlo simulations.
    Qiaamp Dna Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2880 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaamp dna isolation kit/product/Qiagen
    Average 99 stars, based on 2880 article reviews
    Price from $9.99 to $1999.99
    qiaamp dna isolation kit - by Bioz Stars, 2020-09
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    99
    Qiagen dna isolation kit
    Representative image of <t>DNA</t> extraction and PCR analysis of H6H and PMT gene isolated from <t>EMS</t> treated and control explants of H . niger of different samples (500 and 400bp respectively) of (A) DNA extraction isolated from explants treated with different concentrations of EMS and untreated. (B) Depicts the PCR analysis of H6H (500bp) Gene (C) PCR amplification PMT Gene (400bp).
    Dna Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna isolation kit/product/Qiagen
    Average 99 stars, based on 2630 article reviews
    Price from $9.99 to $1999.99
    dna isolation kit - by Bioz Stars, 2020-09
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    99
    Qiagen allprep dna rna isolation kit
    Representative image of <t>DNA</t> extraction and PCR analysis of H6H and PMT gene isolated from <t>EMS</t> treated and control explants of H . niger of different samples (500 and 400bp respectively) of (A) DNA extraction isolated from explants treated with different concentrations of EMS and untreated. (B) Depicts the PCR analysis of H6H (500bp) Gene (C) PCR amplification PMT Gene (400bp).
    Allprep Dna Rna Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 223 article reviews
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    88
    Meridian Life Science isolate genomic dna mini kit
    Representative image of <t>DNA</t> extraction and PCR analysis of H6H and PMT gene isolated from <t>EMS</t> treated and control explants of H . niger of different samples (500 and 400bp respectively) of (A) DNA extraction isolated from explants treated with different concentrations of EMS and untreated. (B) Depicts the PCR analysis of H6H (500bp) Gene (C) PCR amplification PMT Gene (400bp).
    Isolate Genomic Dna Mini Kit, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 88/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    isolate genomic dna mini kit - by Bioz Stars, 2020-09
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    99
    Qiagen genomic dna isolation kit
    Representative image of <t>DNA</t> extraction and PCR analysis of H6H and PMT gene isolated from <t>EMS</t> treated and control explants of H . niger of different samples (500 and 400bp respectively) of (A) DNA extraction isolated from explants treated with different concentrations of EMS and untreated. (B) Depicts the PCR analysis of H6H (500bp) Gene (C) PCR amplification PMT Gene (400bp).
    Genomic Dna Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna isolation kit/product/Qiagen
    Average 99 stars, based on 1267 article reviews
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    genomic dna isolation kit - by Bioz Stars, 2020-09
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    99
    Qiagen plasmid dna isolation kit
    Quantitation of 2-LTR circle Junction <t>DNA</t> by Real-time <t>PCR.</t> Jurkat cells were infected with WT R3B virus and infected cells DNA was isolated at 2h, 24h and 48h post-infection. Virus infected cell DNA was analyzed for amount of 2-LTR circle junction DNA
    Plasmid Dna Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna isolation kit/product/Qiagen
    Average 99 stars, based on 105 article reviews
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    99
    GE Healthcare tissue dna isolation kit
    Construction and screening of PAO1 SALs. (A) Genomic <t>DNA</t> was isolated from P. <t>aeruginosa</t> PAO1 and nebulized to obtain sheared fragments of 200–800 bp. After treatment with exonuclease BAL-31 and Klenow polymerase, the genomic DNA fragments were cloned into the E. coli strain JM109, downstream of the arabinose-inducible promoter P BAD of the pHERD20T vector. (B) E. coli transformants, representing the PAO1 shotgun antisense library (SAL), were arrayed in 96-well microplates and (C) mated with P. aeruginosa PAO1 in the presence of a helper strain (triparental mating). (D) SAL recipient PAO1 exconjugants were selected by spotting on PIA plates supplemented with Cb both in the absence and in the presence of the P BAD inducer arabinose. Recipient PAO1 exconjugant spots were inspected for growth defects following 24 h of incubation at 37°C. (E) The identity of the genomic fragments eliciting growth defects (lethal effects, indicated by a lack of a spot: only with inducer, e.g. clones A4, A8, B5, and E4, and with and without an inducer, e.g. clones A2 and E6; growth impairment, indicated as gray spots: only with an inducer, e.g. clones C2, A6, and B6, and with and without an inducer, e.g. C3 and B8) was determined by sequencing the inserts in the corresponding clones of E. coli SAL.
    Tissue Dna Isolation Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 370 article reviews
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    tissue dna isolation kit - by Bioz Stars, 2020-09
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    93
    LGC Limited mag mini dna isolation kit
    Construction and screening of PAO1 SALs. (A) Genomic <t>DNA</t> was isolated from P. <t>aeruginosa</t> PAO1 and nebulized to obtain sheared fragments of 200–800 bp. After treatment with exonuclease BAL-31 and Klenow polymerase, the genomic DNA fragments were cloned into the E. coli strain JM109, downstream of the arabinose-inducible promoter P BAD of the pHERD20T vector. (B) E. coli transformants, representing the PAO1 shotgun antisense library (SAL), were arrayed in 96-well microplates and (C) mated with P. aeruginosa PAO1 in the presence of a helper strain (triparental mating). (D) SAL recipient PAO1 exconjugants were selected by spotting on PIA plates supplemented with Cb both in the absence and in the presence of the P BAD inducer arabinose. Recipient PAO1 exconjugant spots were inspected for growth defects following 24 h of incubation at 37°C. (E) The identity of the genomic fragments eliciting growth defects (lethal effects, indicated by a lack of a spot: only with inducer, e.g. clones A4, A8, B5, and E4, and with and without an inducer, e.g. clones A2 and E6; growth impairment, indicated as gray spots: only with an inducer, e.g. clones C2, A6, and B6, and with and without an inducer, e.g. C3 and B8) was determined by sequencing the inserts in the corresponding clones of E. coli SAL.
    Mag Mini Dna Isolation Kit, supplied by LGC Limited, used in various techniques. Bioz Stars score: 93/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 64 article reviews
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    mag mini dna isolation kit - by Bioz Stars, 2020-09
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    92
    LGC Limited agowa mag mini dna isolation kit
    Construction and screening of PAO1 SALs. (A) Genomic <t>DNA</t> was isolated from P. <t>aeruginosa</t> PAO1 and nebulized to obtain sheared fragments of 200–800 bp. After treatment with exonuclease BAL-31 and Klenow polymerase, the genomic DNA fragments were cloned into the E. coli strain JM109, downstream of the arabinose-inducible promoter P BAD of the pHERD20T vector. (B) E. coli transformants, representing the PAO1 shotgun antisense library (SAL), were arrayed in 96-well microplates and (C) mated with P. aeruginosa PAO1 in the presence of a helper strain (triparental mating). (D) SAL recipient PAO1 exconjugants were selected by spotting on PIA plates supplemented with Cb both in the absence and in the presence of the P BAD inducer arabinose. Recipient PAO1 exconjugant spots were inspected for growth defects following 24 h of incubation at 37°C. (E) The identity of the genomic fragments eliciting growth defects (lethal effects, indicated by a lack of a spot: only with inducer, e.g. clones A4, A8, B5, and E4, and with and without an inducer, e.g. clones A2 and E6; growth impairment, indicated as gray spots: only with an inducer, e.g. clones C2, A6, and B6, and with and without an inducer, e.g. C3 and B8) was determined by sequencing the inserts in the corresponding clones of E. coli SAL.
    Agowa Mag Mini Dna Isolation Kit, supplied by LGC Limited, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 19 article reviews
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    97
    Norgen Biotek blood dna isolation mini kit
    Construction and screening of PAO1 SALs. (A) Genomic <t>DNA</t> was isolated from P. <t>aeruginosa</t> PAO1 and nebulized to obtain sheared fragments of 200–800 bp. After treatment with exonuclease BAL-31 and Klenow polymerase, the genomic DNA fragments were cloned into the E. coli strain JM109, downstream of the arabinose-inducible promoter P BAD of the pHERD20T vector. (B) E. coli transformants, representing the PAO1 shotgun antisense library (SAL), were arrayed in 96-well microplates and (C) mated with P. aeruginosa PAO1 in the presence of a helper strain (triparental mating). (D) SAL recipient PAO1 exconjugants were selected by spotting on PIA plates supplemented with Cb both in the absence and in the presence of the P BAD inducer arabinose. Recipient PAO1 exconjugant spots were inspected for growth defects following 24 h of incubation at 37°C. (E) The identity of the genomic fragments eliciting growth defects (lethal effects, indicated by a lack of a spot: only with inducer, e.g. clones A4, A8, B5, and E4, and with and without an inducer, e.g. clones A2 and E6; growth impairment, indicated as gray spots: only with an inducer, e.g. clones C2, A6, and B6, and with and without an inducer, e.g. C3 and B8) was determined by sequencing the inserts in the corresponding clones of E. coli SAL.
    Blood Dna Isolation Mini Kit, supplied by Norgen Biotek, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 4 article reviews
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    blood dna isolation mini kit - by Bioz Stars, 2020-09
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    Image Search Results


    Experimental verification of a 16-compartment digital assay with comparison to the performance of qPCR assays. ( A ) The graph shows the results for the same amount of reference DNA suspended in different elution buffers and quantified with conventional qPCR and with the synergistic PCR algorithm. Tests performed on Applied Biosystems 7500 Fast RT System on the IVD Cytomegalovirus PCR kit (GeneProof) according to the prescription. Elution buffers: (1) water, (2) AE elution buffer QIAamp DNA Mini Kit (Quiagen), (3) MBL5 NucleoMag Blood 200 uL (MACHEREY-NAGEL), (4) MagJET Whole Blood Genomic DNA Kit (Thermo Scientific). The gray line shows the expected distribution of results for Real-Time assay. ( B ) The graph shows the result of 24 runs of the synergistic assay, each on 16 partitions of the amplification mix. We conducted two series of 12 assays on the two elution buffers (1 and 4) that provided the largest difference in the result of the conventional qPCR analysis. The gray line shows the expected distribution of results from the synergistic assay used in the experiment, which should provide 60% precision of assessment. This distribution was also verified using 10,000 Monte-Carlo simulations.

    Journal: Scientific Reports

    Article Title: Calibration-free assays on standard real-time PCR devices

    doi: 10.1038/srep44854

    Figure Lengend Snippet: Experimental verification of a 16-compartment digital assay with comparison to the performance of qPCR assays. ( A ) The graph shows the results for the same amount of reference DNA suspended in different elution buffers and quantified with conventional qPCR and with the synergistic PCR algorithm. Tests performed on Applied Biosystems 7500 Fast RT System on the IVD Cytomegalovirus PCR kit (GeneProof) according to the prescription. Elution buffers: (1) water, (2) AE elution buffer QIAamp DNA Mini Kit (Quiagen), (3) MBL5 NucleoMag Blood 200 uL (MACHEREY-NAGEL), (4) MagJET Whole Blood Genomic DNA Kit (Thermo Scientific). The gray line shows the expected distribution of results for Real-Time assay. ( B ) The graph shows the result of 24 runs of the synergistic assay, each on 16 partitions of the amplification mix. We conducted two series of 12 assays on the two elution buffers (1 and 4) that provided the largest difference in the result of the conventional qPCR analysis. The gray line shows the expected distribution of results from the synergistic assay used in the experiment, which should provide 60% precision of assessment. This distribution was also verified using 10,000 Monte-Carlo simulations.

    Article Snippet: Positive control DNA from this kit were diluted in three elution buffers from DNA isolation kits (AE elution buffer QIAamp DNA Mini Kit (Qiagen), MBL5 NucleoMag Blood (MACHEREY-NAGEL) and MagJET Whole Blood Genomic DNA Kit (Thermo Scientific) and water to obtain model samples with 25 000 copies of the target DNA per mL.

    Techniques: Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Amplification

    Representative image of DNA extraction and PCR analysis of H6H and PMT gene isolated from EMS treated and control explants of H . niger of different samples (500 and 400bp respectively) of (A) DNA extraction isolated from explants treated with different concentrations of EMS and untreated. (B) Depicts the PCR analysis of H6H (500bp) Gene (C) PCR amplification PMT Gene (400bp).

    Journal: PLoS ONE

    Article Title: Promoting the accumulation of scopolamine and hyoscyamine in Hyoscyamus niger L. through EMS based mutagenesis

    doi: 10.1371/journal.pone.0231355

    Figure Lengend Snippet: Representative image of DNA extraction and PCR analysis of H6H and PMT gene isolated from EMS treated and control explants of H . niger of different samples (500 and 400bp respectively) of (A) DNA extraction isolated from explants treated with different concentrations of EMS and untreated. (B) Depicts the PCR analysis of H6H (500bp) Gene (C) PCR amplification PMT Gene (400bp).

    Article Snippet: Total genomic DNA was extracted from control and EMS treated samples using a DNA isolation kit (DNeasy- Plant Mini kit-Qiagen, Germany).

    Techniques: DNA Extraction, Polymerase Chain Reaction, Isolation, Amplification

    Quantitation of 2-LTR circle Junction DNA by Real-time PCR. Jurkat cells were infected with WT R3B virus and infected cells DNA was isolated at 2h, 24h and 48h post-infection. Virus infected cell DNA was analyzed for amount of 2-LTR circle junction DNA

    Journal: Methods in molecular biology (Clifton, N.J.)

    Article Title: Analysis of 2-LTR circle junctions of Viral DNA in infected cells

    doi: 10.1007/978-1-59745-170-3_6

    Figure Lengend Snippet: Quantitation of 2-LTR circle Junction DNA by Real-time PCR. Jurkat cells were infected with WT R3B virus and infected cells DNA was isolated at 2h, 24h and 48h post-infection. Virus infected cell DNA was analyzed for amount of 2-LTR circle junction DNA

    Article Snippet: i) Primers specific for 2-LTR circle junctions: 2-LTR forword -- 5' GCC TGG AGC TCT GGC TAA 3' and 2-LTR Reverse – 5' GCC TTG TGT GGT AGA TCC A 3' ( ) for first round PCR and MH535 and MH536 (Section 2.4. i.) for nested 2nd Round PCR. ii) TA cloning kit (Invitrogen) iii) Plasmid DNA isolation kit (QIAGEN) iv) DNA alignment software BioEdit ( ) (BioEdit v7.0.7: ).

    Techniques: Quantitation Assay, Real-time Polymerase Chain Reaction, Infection, Isolation

    Construction and screening of PAO1 SALs. (A) Genomic DNA was isolated from P. aeruginosa PAO1 and nebulized to obtain sheared fragments of 200–800 bp. After treatment with exonuclease BAL-31 and Klenow polymerase, the genomic DNA fragments were cloned into the E. coli strain JM109, downstream of the arabinose-inducible promoter P BAD of the pHERD20T vector. (B) E. coli transformants, representing the PAO1 shotgun antisense library (SAL), were arrayed in 96-well microplates and (C) mated with P. aeruginosa PAO1 in the presence of a helper strain (triparental mating). (D) SAL recipient PAO1 exconjugants were selected by spotting on PIA plates supplemented with Cb both in the absence and in the presence of the P BAD inducer arabinose. Recipient PAO1 exconjugant spots were inspected for growth defects following 24 h of incubation at 37°C. (E) The identity of the genomic fragments eliciting growth defects (lethal effects, indicated by a lack of a spot: only with inducer, e.g. clones A4, A8, B5, and E4, and with and without an inducer, e.g. clones A2 and E6; growth impairment, indicated as gray spots: only with an inducer, e.g. clones C2, A6, and B6, and with and without an inducer, e.g. C3 and B8) was determined by sequencing the inserts in the corresponding clones of E. coli SAL.

    Journal: BMC Microbiology

    Article Title: A shotgun antisense approach to the identification of novel essential genes in Pseudomonas aeruginosa

    doi: 10.1186/1471-2180-14-24

    Figure Lengend Snippet: Construction and screening of PAO1 SALs. (A) Genomic DNA was isolated from P. aeruginosa PAO1 and nebulized to obtain sheared fragments of 200–800 bp. After treatment with exonuclease BAL-31 and Klenow polymerase, the genomic DNA fragments were cloned into the E. coli strain JM109, downstream of the arabinose-inducible promoter P BAD of the pHERD20T vector. (B) E. coli transformants, representing the PAO1 shotgun antisense library (SAL), were arrayed in 96-well microplates and (C) mated with P. aeruginosa PAO1 in the presence of a helper strain (triparental mating). (D) SAL recipient PAO1 exconjugants were selected by spotting on PIA plates supplemented with Cb both in the absence and in the presence of the P BAD inducer arabinose. Recipient PAO1 exconjugant spots were inspected for growth defects following 24 h of incubation at 37°C. (E) The identity of the genomic fragments eliciting growth defects (lethal effects, indicated by a lack of a spot: only with inducer, e.g. clones A4, A8, B5, and E4, and with and without an inducer, e.g. clones A2 and E6; growth impairment, indicated as gray spots: only with an inducer, e.g. clones C2, A6, and B6, and with and without an inducer, e.g. C3 and B8) was determined by sequencing the inserts in the corresponding clones of E. coli SAL.

    Article Snippet: Construction and screening of PAO1 shotgun antisense libraries Genomic DNA was isolated from P. aeruginosa PAO1 using an illustra GenomicPrep Cells and Tissue DNA Isolation Kit (GE Healthcare).

    Techniques: Isolation, Clone Assay, Plasmid Preparation, Incubation, Sequencing