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  • 99
    Millipore dna isolation isolation
    FIGURE 2. (A) T/HS lymph but not T/SS lymph or medium induced <t>DNA</t> fragmentation. HUVECs were incubated with lymph for 4 hours, after which genomic DNA was isolated, fractionated on a 1.5% agarose gel and stained with ethidium bromide. The hallmark DNA step-laddering pattern characteristic of apoptosis is present only in the HUVECS incubated with T/HS lymph. These results are representative of 3 independent studies (M, marker). (B) The number of free nucleosomes generated in HUVECs after 4-hour incubation with T/HS lymph was increased compared with HUVECs incubated in medium or T/SS lymph. Nuclear extracts were prepared using a Nucleosome ELISA kit (Oncogene Research). Optical density (OD) correlates with the number of free nucleosomes. Data expressed as mean ± SD. * P
    Dna Isolation Isolation, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna isolation isolation/product/Millipore
    Average 99 stars, based on 60 article reviews
    Price from $9.99 to $1999.99
    dna isolation isolation - by Bioz Stars, 2020-08
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    99
    Millipore dna isolation
    FIGURE 2. (A) T/HS lymph but not T/SS lymph or medium induced <t>DNA</t> fragmentation. HUVECs were incubated with lymph for 4 hours, after which genomic DNA was isolated, fractionated on a 1.5% agarose gel and stained with ethidium bromide. The hallmark DNA step-laddering pattern characteristic of apoptosis is present only in the HUVECS incubated with T/HS lymph. These results are representative of 3 independent studies (M, marker). (B) The number of free nucleosomes generated in HUVECs after 4-hour incubation with T/HS lymph was increased compared with HUVECs incubated in medium or T/SS lymph. Nuclear extracts were prepared using a Nucleosome ELISA kit (Oncogene Research). Optical density (OD) correlates with the number of free nucleosomes. Data expressed as mean ± SD. * P
    Dna Isolation, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna isolation/product/Millipore
    Average 99 stars, based on 194 article reviews
    Price from $9.99 to $1999.99
    dna isolation - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Millipore genomic dna isolation
    Integration of gene expression and methylome data. a Scatterplot showing mean gene expression and boxplot showing mean <t>DNA</t> methylation in differentially methylated regions (DMRs) in treated group for DMRs in promoters ( a ) and DMRs in gene bodies ( b ), with lines representing a linear trend. Bars in the box plot correspond to the median. The lower and upper hinges correspond to the first and third quartiles (the 25th and 75th percentiles). The lower/upper whisker extends from the hinge to the smallest/largest value no further than 1.5 * IQR (inter quartile range) from the hinges. c – d Scatterplots displaying the effect of lipopolysaccharide (LPS) on the transcriptome and the methylome when compared to the control group; change in gene expression (log 2 Fold Change) is plotted against change in DNA methylation for c promoters of 12,115 genes and ( d ) gene bodies of 13,263 genes. Highlighted points denote genes with |ΔMethylation| > 5% and | Δexpression log 2 FC| > 1; hypermethylated/increased expression (yellow), hypermethylated/lower expression (blue), hypomethylated/increased expression (green) and hypomethylated/lower expression (red)
    Genomic Dna Isolation, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna isolation/product/Millipore
    Average 99 stars, based on 99 article reviews
    Price from $9.99 to $1999.99
    genomic dna isolation - by Bioz Stars, 2020-08
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    99
    Millipore isolates total dna
    Integration of gene expression and methylome data. a Scatterplot showing mean gene expression and boxplot showing mean <t>DNA</t> methylation in differentially methylated regions (DMRs) in treated group for DMRs in promoters ( a ) and DMRs in gene bodies ( b ), with lines representing a linear trend. Bars in the box plot correspond to the median. The lower and upper hinges correspond to the first and third quartiles (the 25th and 75th percentiles). The lower/upper whisker extends from the hinge to the smallest/largest value no further than 1.5 * IQR (inter quartile range) from the hinges. c – d Scatterplots displaying the effect of lipopolysaccharide (LPS) on the transcriptome and the methylome when compared to the control group; change in gene expression (log 2 Fold Change) is plotted against change in DNA methylation for c promoters of 12,115 genes and ( d ) gene bodies of 13,263 genes. Highlighted points denote genes with |ΔMethylation| > 5% and | Δexpression log 2 FC| > 1; hypermethylated/increased expression (yellow), hypermethylated/lower expression (blue), hypomethylated/increased expression (green) and hypomethylated/lower expression (red)
    Isolates Total Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    FIGURE 2. (A) T/HS lymph but not T/SS lymph or medium induced DNA fragmentation. HUVECs were incubated with lymph for 4 hours, after which genomic DNA was isolated, fractionated on a 1.5% agarose gel and stained with ethidium bromide. The hallmark DNA step-laddering pattern characteristic of apoptosis is present only in the HUVECS incubated with T/HS lymph. These results are representative of 3 independent studies (M, marker). (B) The number of free nucleosomes generated in HUVECs after 4-hour incubation with T/HS lymph was increased compared with HUVECs incubated in medium or T/SS lymph. Nuclear extracts were prepared using a Nucleosome ELISA kit (Oncogene Research). Optical density (OD) correlates with the number of free nucleosomes. Data expressed as mean ± SD. * P

    Journal: Annals of Surgery

    Article Title: Trauma-Hemorrhagic Shock Mesenteric Lymph Induces Endothelial Apoptosis That Involves Both Caspase-Dependent and Caspase-Independent Mechanisms

    doi: 10.1097/01.sla.0000129341.94219.cf

    Figure Lengend Snippet: FIGURE 2. (A) T/HS lymph but not T/SS lymph or medium induced DNA fragmentation. HUVECs were incubated with lymph for 4 hours, after which genomic DNA was isolated, fractionated on a 1.5% agarose gel and stained with ethidium bromide. The hallmark DNA step-laddering pattern characteristic of apoptosis is present only in the HUVECS incubated with T/HS lymph. These results are representative of 3 independent studies (M, marker). (B) The number of free nucleosomes generated in HUVECs after 4-hour incubation with T/HS lymph was increased compared with HUVECs incubated in medium or T/SS lymph. Nuclear extracts were prepared using a Nucleosome ELISA kit (Oncogene Research). Optical density (OD) correlates with the number of free nucleosomes. Data expressed as mean ± SD. * P

    Article Snippet: Using a DNA Isolation Kit (Oncogene Research Products, Boston MA) low molecular weight DNA extracts were prepared.

    Techniques: Incubation, Isolation, Agarose Gel Electrophoresis, Staining, Marker, Generated, Enzyme-linked Immunosorbent Assay

    MIEN1 expression upon knockdown of DNA methyltransferases in PWR-1E ( A – C ) MIEN1 expression upon different DNMT knockdown in PWR-1E cells as shown by (A–B) qPCR and ( C ) Western Blotting. MIEN1 expression upon different DNMT knockdown in DU-145 cells as shown by ( D ) qPCR and ( E ) Western Blotting. The P -values were computed using One-way ANOVA to compare all the groups and then followed by Tukey's Post Hoc comparison to obtain the pairwise significances between the treatments. *** P ≤ 0.001; ** P ≤ 0.01.

    Journal: Oncotarget

    Article Title: MIEN1 is tightly regulated by SINE Alu methylation in its promoter

    doi: 10.18632/oncotarget.11675

    Figure Lengend Snippet: MIEN1 expression upon knockdown of DNA methyltransferases in PWR-1E ( A – C ) MIEN1 expression upon different DNMT knockdown in PWR-1E cells as shown by (A–B) qPCR and ( C ) Western Blotting. MIEN1 expression upon different DNMT knockdown in DU-145 cells as shown by ( D ) qPCR and ( E ) Western Blotting. The P -values were computed using One-way ANOVA to compare all the groups and then followed by Tukey's Post Hoc comparison to obtain the pairwise significances between the treatments. *** P ≤ 0.001; ** P ≤ 0.01.

    Article Snippet: Genomic DNA isolation, bisulfite modification and sequencing Genomic DNA from PWR-1E, LNCaP, DU-145 and PC-3 cells was isolated using the Genomic DNA isolation kit (Sigma-Aldrich; St. Louis, MO, USA) according to manufacturer's protocol.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    Integration of gene expression and methylome data. a Scatterplot showing mean gene expression and boxplot showing mean DNA methylation in differentially methylated regions (DMRs) in treated group for DMRs in promoters ( a ) and DMRs in gene bodies ( b ), with lines representing a linear trend. Bars in the box plot correspond to the median. The lower and upper hinges correspond to the first and third quartiles (the 25th and 75th percentiles). The lower/upper whisker extends from the hinge to the smallest/largest value no further than 1.5 * IQR (inter quartile range) from the hinges. c – d Scatterplots displaying the effect of lipopolysaccharide (LPS) on the transcriptome and the methylome when compared to the control group; change in gene expression (log 2 Fold Change) is plotted against change in DNA methylation for c promoters of 12,115 genes and ( d ) gene bodies of 13,263 genes. Highlighted points denote genes with |ΔMethylation| > 5% and | Δexpression log 2 FC| > 1; hypermethylated/increased expression (yellow), hypermethylated/lower expression (blue), hypomethylated/increased expression (green) and hypomethylated/lower expression (red)

    Journal: BMC Genomics

    Article Title: LPS-treatment of bovine endometrial epithelial cells causes differential DNA methylation of genes associated with inflammation and endometrial function

    doi: 10.1186/s12864-020-06777-7

    Figure Lengend Snippet: Integration of gene expression and methylome data. a Scatterplot showing mean gene expression and boxplot showing mean DNA methylation in differentially methylated regions (DMRs) in treated group for DMRs in promoters ( a ) and DMRs in gene bodies ( b ), with lines representing a linear trend. Bars in the box plot correspond to the median. The lower and upper hinges correspond to the first and third quartiles (the 25th and 75th percentiles). The lower/upper whisker extends from the hinge to the smallest/largest value no further than 1.5 * IQR (inter quartile range) from the hinges. c – d Scatterplots displaying the effect of lipopolysaccharide (LPS) on the transcriptome and the methylome when compared to the control group; change in gene expression (log 2 Fold Change) is plotted against change in DNA methylation for c promoters of 12,115 genes and ( d ) gene bodies of 13,263 genes. Highlighted points denote genes with |ΔMethylation| > 5% and | Δexpression log 2 FC| > 1; hypermethylated/increased expression (yellow), hypermethylated/lower expression (blue), hypomethylated/increased expression (green) and hypomethylated/lower expression (red)

    Article Snippet: LPS challenge and genomic DNA isolation According to the guide from Sigma, LPS (L2630-10MG O111:B4, Sigma-Aldrich) was reconstituted by 2 mL LAL reagent water (W50–640, Lonza, Walkersville, MD, USA) to a stock concentration of 5 mg/mL.

    Techniques: Expressing, DNA Methylation Assay, Methylation, Whisker Assay

    LPS effects on DNA methylation in bovine endometrial epithelial cells (bEECs). a Principal component analysis displaying overall methylation profiles across all samples. The first dimension explained 21% variation and separated Cow1 from Cow2 and Cow3. The second dimension explained 16% variation, separated both Cow2 versus Cow3. b Venn diagram displaying overlapping differentially methylated regions (DMRs) from 24 h sample groups: 0 μg vs. 2 μg (pink), 0 μg vs. 8 μg (blue), and 0 μg vs. 2 μg + 8 μg (green). c Heatmap of significant DMRs (1291) showing similar methylation trend for the analyses performed in ( b ). The scale shows hypermethylated (red) and hypomethylated (blue) levels for each DMR. d Bar plot showing distribution of the percent of hyper and hypomethylated DMRs when comparing time 0 h and 24 h in controls, and 24 h control with 2 μg or 8 μg, and 2 μg + 8 μg combined LPS groups. Top bar shows a similar pattern for total DMRs identified in 2 μg, 8 μg, and 2 μg + 8 μg analysis

    Journal: BMC Genomics

    Article Title: LPS-treatment of bovine endometrial epithelial cells causes differential DNA methylation of genes associated with inflammation and endometrial function

    doi: 10.1186/s12864-020-06777-7

    Figure Lengend Snippet: LPS effects on DNA methylation in bovine endometrial epithelial cells (bEECs). a Principal component analysis displaying overall methylation profiles across all samples. The first dimension explained 21% variation and separated Cow1 from Cow2 and Cow3. The second dimension explained 16% variation, separated both Cow2 versus Cow3. b Venn diagram displaying overlapping differentially methylated regions (DMRs) from 24 h sample groups: 0 μg vs. 2 μg (pink), 0 μg vs. 8 μg (blue), and 0 μg vs. 2 μg + 8 μg (green). c Heatmap of significant DMRs (1291) showing similar methylation trend for the analyses performed in ( b ). The scale shows hypermethylated (red) and hypomethylated (blue) levels for each DMR. d Bar plot showing distribution of the percent of hyper and hypomethylated DMRs when comparing time 0 h and 24 h in controls, and 24 h control with 2 μg or 8 μg, and 2 μg + 8 μg combined LPS groups. Top bar shows a similar pattern for total DMRs identified in 2 μg, 8 μg, and 2 μg + 8 μg analysis

    Article Snippet: LPS challenge and genomic DNA isolation According to the guide from Sigma, LPS (L2630-10MG O111:B4, Sigma-Aldrich) was reconstituted by 2 mL LAL reagent water (W50–640, Lonza, Walkersville, MD, USA) to a stock concentration of 5 mg/mL.

    Techniques: DNA Methylation Assay, Methylation