Journal: Frontiers in Pharmacology

Article Title: Dexmedetomidine alleviates lung ischemia-reperfusion injury by inhibiting cuproptosis: an in vivo study

doi: 10.3389/fphar.2025.1562535

Figure Lengend Snippet: WB results of cuproptosis-related protein expression. (A) Western blots for FDX 1, SLC 31 A1, LIAS, DLST, DLD, and SDHB in rat lung tissue. (B–G) relative levels of FDX 1, SLC 31 A1, LIAS, DLST, DLD, and SDHB in rat lung tissue (n = 3). ## p < 0.05 vs. control group; ** p < 0.05 vs. I/R group.

Article Snippet: Membranes were incubated overnight at 4°C with primary antibodies against: lipoic acid (Abcam, 1:1,000), FDX1 (Abcam, 1:1,000), LIAS (Proteintech, 1:1,000), SDHB (Proteintech, 1:1,000), DLAT (Proteintech, 1:1,000), DLD (Proteintech, 1:1,000), SLC31A1 (MCE, 1:1,000), DLST (MCE, 1:1,000), and β-actin (Sevier, 1:5,000).

Techniques: Expressing, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Dexmedetomidine alleviates lung ischemia-reperfusion injury by inhibiting cuproptosis: an in vivo study

doi: 10.3389/fphar.2025.1562535

Figure Lengend Snippet: Immunohistochemical and WB results of lipoacylated proteins. (A–C) representative images of lipoacylated protein immunohistochemical staining in rat lung tissue. (D) Western blots for lip-DLAT and lip-DLST. (E, F) relative levels of lip-DLAT and lip-DLST in rats lung tissue (n = 3). ## p < 0.05 vs. control group; ** p < 0.05 vs. I/R group.

Article Snippet: Membranes were incubated overnight at 4°C with primary antibodies against: lipoic acid (Abcam, 1:1,000), FDX1 (Abcam, 1:1,000), LIAS (Proteintech, 1:1,000), SDHB (Proteintech, 1:1,000), DLAT (Proteintech, 1:1,000), DLD (Proteintech, 1:1,000), SLC31A1 (MCE, 1:1,000), DLST (MCE, 1:1,000), and β-actin (Sevier, 1:5,000).

Techniques: Immunohistochemical staining, Staining, Western Blot, Control

Journal: Metabolomics

Article Title: Effect of everolimus on the glucose metabolic pathway in mouse skeletal muscle cells (C2C12)

doi: 10.1007/s11306-017-1236-5

Figure Lengend Snippet: List of genes measured by real-time RT-PCR in this study. TaqMan ® gene expression assays. The following TaqMan probes (Thermo Fisher Scientific; Waltham, MA, USA) were used in our study

Article Snippet: Dlst (Mm00513470_m1) , Eno1 (Mm01619597_g1) , Fh1 (Mm01321349_m1).

Techniques: Quantitative RT-PCR, Gene Expression

Journal: Metabolomics

Article Title: Effect of everolimus on the glucose metabolic pathway in mouse skeletal muscle cells (C2C12)

doi: 10.1007/s11306-017-1236-5

Figure Lengend Snippet: Metabolic profiling of intracellular glycometabolism. Changes in metabolite levels after 48 h exposure to everolimus ( closed bars , EVE) were compared with those without everolimus at 48 h (Control). The data represent the mean ± S.D. (n = 3). Statistically significant differences between the EVE and control: *p < 0.05; **p < 0.01 (Student’s t -test). Abbreviations: G6P glucose 6-phosphate, F6P fructose 6-phosphate, F1,6P fructose 1,6-bisphosphate, GAP glyceraldehyde phosphate, DHAP dihydroxyacetone phosphate, 1,3-Bis-PG 1,3-Bis phosphoglycerate, 3PG 3-phosphoglycerate, 2PG 2-phosphoglycerate, PEP phosphoenolpyruvate, Ac-CoA acetyl CoA, Ru5P ribulose 5-phosphate, R5P ribose 5-phosphate, Xu5P xylulose 5-phosphate, mTOR mammalian target of rapamycin, HIF1a hypoxia-inducible factor-1α, GLUT glucose transporter, HK1 hexokinase-1, GPI glucose-6-phosphate isomerase, PFKM 6-phosphofructokinase, ALDOA aldolase, TPI1P2 triosephosphate isomerase, GAPDH glyceraldehyde 3-phosphate dehydrogenase, PGK1 phosphoglycerate kinase-1, ENO1 enolase-1, PKM2 pyruvate kinase-2, LDHA _lactate dehydrogenase A, PDHA1 pyruvate dehydrogenase α1, G6PD 6-phosphate dehydrogenase, PGD 6-phosphogluconate dehydrogenase, RPIA ribose-5-phosphate isomerase A, TKT transketolase, TALDO1 transaldolase-1, CS citrate synthase, ACO1 aconitase-1, IDH1 isocitrate dehydrogenase-1, OGDH α-ketoglutarate dehydrogenase, DLST dihydrolipoamide succinyltransferase, SUCLG2 succinyl-CoA ligase, SDHA succinate dehydrogenase A, FH fumarate hydratase, MDH1 malate dehydrogenase-1

Article Snippet: Dlst (Mm00513470_m1) , Eno1 (Mm01619597_g1) , Fh1 (Mm01321349_m1).

Techniques: Control

Journal: Journal of Cancer Prevention

Article Title: Breast Cancer Selective Disruption of Actin Cytoskeleton by Diallyl Trisulfide

doi: 10.15430/jcp.2022.27.2.101

Figure Lengend Snippet: Figure 6. Effect of DATS on expression of tricarboxylic acid cycle-associated proteins. (A) Reactome pathway enrichment analysis of downreg- ulated genes by DATS treatment in MCF-10A and SK-BR-3 cell lines. (B) Immunoblot analysis for ACO2 and DLST in MCF-10A and SK-BR-3 cells treated with DMSO (control) or indicated doses of DATS for specified time points. The numbers above bands represent fold change in expression relative to corresponding DMSO-treated controls. (C) Quantification of ACO2 and DLST expression in SK-BR-3 cells. Results combined from three independent experiments are shown as mean ± SD (n = 3) and statistical analysis was done by one-way ANOVA followed by Dunnett’s test. DATS, diallyl trisulfide; DMSO, dimethyl sulfoxide, DLST, dihydrolipoamide S-succinyltransferase; ACO2, aconitase 2; HDR, Homology directed repair; ATR, ataxia telangiectasia and Rad3 related; AP, abasic sites; PCNA, proliferating cell nuclear antigen.

Article Snippet: Anti-aconitase 2 (ACO2) antibody and anti-dihydrolipoamide S-succinyltransferase (DLST) antibody were from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Cell reports

Article Title: A lncRNA-mediated metabolic rewiring of cell senescence.

doi: 10.1016/j.celrep.2025.115747

Figure Lengend Snippet: Figure 4. sin-lncRNA interacts with DLST and controls its subcellular localization (A) RNA-FISH based on locked nucleic acid (LNA) technology on fixed IMR90 ER:RAS cells depleted or not of sin-lncRNA (siRNA_sin-lncRNA#1/3) and treated for 5 days with 4OHT. Two different LNAs were used. Incubation without LNA probe (‘‘No_probe’’) was used as control. Scale bar: 10 μm. (B) RT-qPCR analysis of a set of genes after mitochondria purification from proliferating (−4OHT) or senescent cells (+4OHT) using Mitotracker (green, 488) staining and FACS sorter. The graph shows a representative experiment. (C) Schematic representation of the TCA cycle that takes place in the mitochondria. DLST enzyme is highlighted in red. (D) RNA immunoprecipitation analysis monitoring DLST binding to sin-lncRNA, MALAT1, and GAPDH RNA in control and sin-lncRNA-depleted (siRNA#1/3) senescent IMR90 ER:RAS, using anti-DLST antibody or IgG as a negative control. The graph represents the mean ± SD (n = 2). (E) Left, western blot analysis of DLST cellular fractionation in control and sin-lncRNA-depleted (siRNA#1/3) senescent IMR90 ER:RAS. GAPDH and H3 were used as cytoplasmic and nuclear controls, respectively. Right, percentage of nuclear and cytoplasmic distribution of DLST normalized to GAPDH (cyto) or H3 (nucl). The graph represents the mean ± SD (n = 3). Two-tailed Student’s t test, **p < 0.01.

Article Snippet: After centrifugation, the samples were precleared with Dynabeads Protein A (Invitrogen) for 1 h. One per cent of the sample was used as the input control and the remaining extracts were incubated with 10 μg DLST antibody (Bethyl) or IgG at 4 ◦C overnight.

Techniques: Incubation, Control, Quantitative RT-PCR, Purification, Staining, RNA Immunoprecipitation, Binding Assay, Negative Control, Western Blot, Cell Fractionation, Two Tailed Test