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  • dll1  (Abcam)
    96
    Abcam dll1
    MPDZ recruits <t>DLL1</t> and DLL4 to Nectin-2. ( A ) DLL1 and DLL4 full length and such lacking the PDZ-binding site (ΔPDZ) constructs containing a mCherry tag were expressed in HUVEC. Cells were stained with antibodies against DLL1 or DLL4. DLL1 and DLL4 surface expression of mCherry positive cells was analyzed by flow cytometry. n = 3; *, p
    Dll1, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dll1/product/Abcam
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dll1 - by Bioz Stars, 2021-06
    96/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology dll1
    Overexpression of <t>Dll1</t> promotes tumor metastasis in vivo . A , B16-Dll1 or B16-GFP tumor cells were injected into the tail vein of normal mice. Lungs were dissected and photographed 15 days later. Tumor nodules are observed on the surface of lungs (red arrows). B , Tumor nodules on the surface of the lung were counted under a stereomicroscope and compared. C , Lung weight index was calculated as the ratio of lung weight versus body weight of host mice, and was compared between the two groups. D , Lungs in A were sectioned and photographed with H E staining. T indicates the tumor foci in lungs. E , Tumor area in lung section was assessed by the Image-Pro Plus Phase 6 Imaging System and nomalized to total lung area. Data are reported as means±SD. **P
    Dll1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dll1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dll1 - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Angiocrine dll1
    Endothelial <t>Dll1</t> regulates macrophage maturation and arteriogenesis. a Analysis of cell populations by flow cytometry in ischemic muscle of induced endothelial Dll1 mutant mice ( Dll1 iΔEC ) and control mice. n = 8 mice/group, error bars represent s.e.m. * p
    Dll1, supplied by Angiocrine, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dll1/product/Angiocrine
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dll1 - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    N/A
    Notch signalling pathways play key roles in cell fate determination and differentiation in many tissues during embryonic and postnatal development Notch ligands are divided into two subclasses the delta and
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    N/A
    The DLL1 Antibody from Novus Biologicals is a rabbit polyclonal antibody to DLL1 This antibody reacts with human mouse rat chicken The DLL1 Antibody has been validated for the following
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    Image Search Results


    MPDZ recruits DLL1 and DLL4 to Nectin-2. ( A ) DLL1 and DLL4 full length and such lacking the PDZ-binding site (ΔPDZ) constructs containing a mCherry tag were expressed in HUVEC. Cells were stained with antibodies against DLL1 or DLL4. DLL1 and DLL4 surface expression of mCherry positive cells was analyzed by flow cytometry. n = 3; *, p

    Journal: eLife

    Article Title: MPDZ promotes DLL4-induced Notch signaling during angiogenesis

    doi: 10.7554/eLife.32860

    Figure Lengend Snippet: MPDZ recruits DLL1 and DLL4 to Nectin-2. ( A ) DLL1 and DLL4 full length and such lacking the PDZ-binding site (ΔPDZ) constructs containing a mCherry tag were expressed in HUVEC. Cells were stained with antibodies against DLL1 or DLL4. DLL1 and DLL4 surface expression of mCherry positive cells was analyzed by flow cytometry. n = 3; *, p

    Article Snippet: After blocking with 3% BSA in PBS, cells were incubated with the primary antibodies against MPDZ (HPA020255, Sigma-Aldrich, 1:50), DLL1 (ab85346, abcam, 1:100), DLL4 (WH0054567M4, Sigma-Aldrich, 1:100), Nectin-2 (ab135246, abcam, 1:50), Nectin-2 (sc-32804, Santa Cruz Biotechnology, 1:50) over night at 4°C and secondary antibodies (Thermo Fisher Scientific, 1:400) for 1 hr at room temperature.

    Techniques: Binding Assay, Construct, Staining, Expressing, Flow Cytometry, Cytometry

    MPDZ interacts with DLL1 and DLL4. ( A, B ) HEK293T cells were transfected with Citrine-MPDZ together with HA-tagged DLL1, HA-tagged DLL1 ΔPDZ (lacking the PDZ-binding site), Flag-tagged DLL4 or Flag-tagged DLL4 ΔPDZ . Antibodies against Citrine were used to immunoprecipitate Citrine-MPDZ. HA and FLAG-tagged proteins as well as MPDZ were detected by immunoblot (IB). Scheme shows structures of the constructs used for co-immunoprecipitation. Input, 10% of the immunoprecipitate. Cit-MPDZ, Citrine-MPDZ; IP, immunoprecipitation; neg.ctrl., negative control. ( C, D ) MPDZ was co-expressed with either DLL1 or DLL4 in primary endothelial cells (HUVEC). DLL1 and DLL4 were pulled down by using specific antibodies. DLL1, DLL4 and MPDZ were detected by immunoblot (IB). Input, 5% of the immunoprecipitate. IP, immunoprecipitation; neg.ctrl., negative control. ( E ) HUVEC were either transduced with adenovirus expressing GFP (ctrl) or MPDZ (MPDZ). Expression level of Notch target genes HEY1 , HEY2 and HES1 were analyzed by qPCR 48 hr after transduction. Data are presented as mean ±SD. n = 4; *, p

    Journal: eLife

    Article Title: MPDZ promotes DLL4-induced Notch signaling during angiogenesis

    doi: 10.7554/eLife.32860

    Figure Lengend Snippet: MPDZ interacts with DLL1 and DLL4. ( A, B ) HEK293T cells were transfected with Citrine-MPDZ together with HA-tagged DLL1, HA-tagged DLL1 ΔPDZ (lacking the PDZ-binding site), Flag-tagged DLL4 or Flag-tagged DLL4 ΔPDZ . Antibodies against Citrine were used to immunoprecipitate Citrine-MPDZ. HA and FLAG-tagged proteins as well as MPDZ were detected by immunoblot (IB). Scheme shows structures of the constructs used for co-immunoprecipitation. Input, 10% of the immunoprecipitate. Cit-MPDZ, Citrine-MPDZ; IP, immunoprecipitation; neg.ctrl., negative control. ( C, D ) MPDZ was co-expressed with either DLL1 or DLL4 in primary endothelial cells (HUVEC). DLL1 and DLL4 were pulled down by using specific antibodies. DLL1, DLL4 and MPDZ were detected by immunoblot (IB). Input, 5% of the immunoprecipitate. IP, immunoprecipitation; neg.ctrl., negative control. ( E ) HUVEC were either transduced with adenovirus expressing GFP (ctrl) or MPDZ (MPDZ). Expression level of Notch target genes HEY1 , HEY2 and HES1 were analyzed by qPCR 48 hr after transduction. Data are presented as mean ±SD. n = 4; *, p

    Article Snippet: After blocking with 3% BSA in PBS, cells were incubated with the primary antibodies against MPDZ (HPA020255, Sigma-Aldrich, 1:50), DLL1 (ab85346, abcam, 1:100), DLL4 (WH0054567M4, Sigma-Aldrich, 1:100), Nectin-2 (ab135246, abcam, 1:50), Nectin-2 (sc-32804, Santa Cruz Biotechnology, 1:50) over night at 4°C and secondary antibodies (Thermo Fisher Scientific, 1:400) for 1 hr at room temperature.

    Techniques: Transfection, Binding Assay, Construct, Immunoprecipitation, Negative Control, Transduction, Expressing, Real-time Polymerase Chain Reaction

    Overexpression of Dll1 promotes tumor metastasis in vivo . A , B16-Dll1 or B16-GFP tumor cells were injected into the tail vein of normal mice. Lungs were dissected and photographed 15 days later. Tumor nodules are observed on the surface of lungs (red arrows). B , Tumor nodules on the surface of the lung were counted under a stereomicroscope and compared. C , Lung weight index was calculated as the ratio of lung weight versus body weight of host mice, and was compared between the two groups. D , Lungs in A were sectioned and photographed with H E staining. T indicates the tumor foci in lungs. E , Tumor area in lung section was assessed by the Image-Pro Plus Phase 6 Imaging System and nomalized to total lung area. Data are reported as means±SD. **P

    Journal: Brazilian Journal of Medical and Biological Research

    Article Title: Notch ligand Delta-like 1 promotes the metastasis of melanoma by enhancing tumor adhesion

    doi: 10.1590/1414-431X20143368

    Figure Lengend Snippet: Overexpression of Dll1 promotes tumor metastasis in vivo . A , B16-Dll1 or B16-GFP tumor cells were injected into the tail vein of normal mice. Lungs were dissected and photographed 15 days later. Tumor nodules are observed on the surface of lungs (red arrows). B , Tumor nodules on the surface of the lung were counted under a stereomicroscope and compared. C , Lung weight index was calculated as the ratio of lung weight versus body weight of host mice, and was compared between the two groups. D , Lungs in A were sectioned and photographed with H E staining. T indicates the tumor foci in lungs. E , Tumor area in lung section was assessed by the Image-Pro Plus Phase 6 Imaging System and nomalized to total lung area. Data are reported as means±SD. **P

    Article Snippet: The primary antibodies included goat polyclonal IgG antibody to Dll1 and rabbit polyclonal IgG antibodies to Hes1, N-cadherin, or E-cadherin (Santa Cruz Biotechnology, USA). β-actin was detected simultaneously using 1:5000 dilution of monoclonal mouse anti-β-actin antibody (Sigma, USA) as a loading control.

    Techniques: Over Expression, In Vivo, Injection, Mouse Assay, Staining, Imaging

    Dll1-mediated Notch activation induced N-cadherin (cad) expression. The expressions of N-cadherin ( A ) and E-cadherin ( B ) were assessed by real-time RT-PCR, with β-actin as a reference control. C , The protein levels of Hes1, N-cadherin, E-cadherin in tumor cells were examined with Western blot. Data are reported as means±SD. **P

    Journal: Brazilian Journal of Medical and Biological Research

    Article Title: Notch ligand Delta-like 1 promotes the metastasis of melanoma by enhancing tumor adhesion

    doi: 10.1590/1414-431X20143368

    Figure Lengend Snippet: Dll1-mediated Notch activation induced N-cadherin (cad) expression. The expressions of N-cadherin ( A ) and E-cadherin ( B ) were assessed by real-time RT-PCR, with β-actin as a reference control. C , The protein levels of Hes1, N-cadherin, E-cadherin in tumor cells were examined with Western blot. Data are reported as means±SD. **P

    Article Snippet: The primary antibodies included goat polyclonal IgG antibody to Dll1 and rabbit polyclonal IgG antibodies to Hes1, N-cadherin, or E-cadherin (Santa Cruz Biotechnology, USA). β-actin was detected simultaneously using 1:5000 dilution of monoclonal mouse anti-β-actin antibody (Sigma, USA) as a loading control.

    Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Western Blot

    Dll1 overexpression enhanced the adhesion capacity of tumor cells. A , Growth colonies of tumor cells were observed by seeding at low density. B , Plate adhesion assay. The bound tumor cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and compared. C , Endothelial cell (EC) adhesion assay. GFP-labeled tumor cells that adhered to EC were analyzed by flow cytometry. The total number of GFP-labeled cells was calculated based on flow cytometry shown in C. Data are reported as means±SD. **P

    Journal: Brazilian Journal of Medical and Biological Research

    Article Title: Notch ligand Delta-like 1 promotes the metastasis of melanoma by enhancing tumor adhesion

    doi: 10.1590/1414-431X20143368

    Figure Lengend Snippet: Dll1 overexpression enhanced the adhesion capacity of tumor cells. A , Growth colonies of tumor cells were observed by seeding at low density. B , Plate adhesion assay. The bound tumor cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and compared. C , Endothelial cell (EC) adhesion assay. GFP-labeled tumor cells that adhered to EC were analyzed by flow cytometry. The total number of GFP-labeled cells was calculated based on flow cytometry shown in C. Data are reported as means±SD. **P

    Article Snippet: The primary antibodies included goat polyclonal IgG antibody to Dll1 and rabbit polyclonal IgG antibodies to Hes1, N-cadherin, or E-cadherin (Santa Cruz Biotechnology, USA). β-actin was detected simultaneously using 1:5000 dilution of monoclonal mouse anti-β-actin antibody (Sigma, USA) as a loading control.

    Techniques: Over Expression, Cell Adhesion Assay, Labeling, Flow Cytometry, Cytometry

    Overexpression of Dll1 led to tumor cell arrest in lung. A , Vessels of mouse lung were labeled with Evans blue (red) and sectioned, arrested GFP-labeled tumor cells (green) were detected with fluorescent microscope, at 40× magnification. Insets were amplified by laser scanning microscope at 200× magnification. Arrows indicate the vessels of mouse lung section. B , Average numbers of GFP-labeled tumor cells were counted in lung sections with the Image-Pro Plus Phase 6 Imaging System and compared. Data are reported as means±SD. **P

    Journal: Brazilian Journal of Medical and Biological Research

    Article Title: Notch ligand Delta-like 1 promotes the metastasis of melanoma by enhancing tumor adhesion

    doi: 10.1590/1414-431X20143368

    Figure Lengend Snippet: Overexpression of Dll1 led to tumor cell arrest in lung. A , Vessels of mouse lung were labeled with Evans blue (red) and sectioned, arrested GFP-labeled tumor cells (green) were detected with fluorescent microscope, at 40× magnification. Insets were amplified by laser scanning microscope at 200× magnification. Arrows indicate the vessels of mouse lung section. B , Average numbers of GFP-labeled tumor cells were counted in lung sections with the Image-Pro Plus Phase 6 Imaging System and compared. Data are reported as means±SD. **P

    Article Snippet: The primary antibodies included goat polyclonal IgG antibody to Dll1 and rabbit polyclonal IgG antibodies to Hes1, N-cadherin, or E-cadherin (Santa Cruz Biotechnology, USA). β-actin was detected simultaneously using 1:5000 dilution of monoclonal mouse anti-β-actin antibody (Sigma, USA) as a loading control.

    Techniques: Over Expression, Labeling, Microscopy, Amplification, Laser-Scanning Microscopy, Imaging

    Overexpression of Dll1 activated Notch signaling in B16 cells. A , Quantitative PCR revealed expression of Dll1 mRNA in transfected cells. B , Expression of Notch down-stream gene Hes1 was observed in transfected B16 tumor cells. C , Dll1 protein was detected by Western blot in B16 tumor cells. Data are reported as means±SD. **P

    Journal: Brazilian Journal of Medical and Biological Research

    Article Title: Notch ligand Delta-like 1 promotes the metastasis of melanoma by enhancing tumor adhesion

    doi: 10.1590/1414-431X20143368

    Figure Lengend Snippet: Overexpression of Dll1 activated Notch signaling in B16 cells. A , Quantitative PCR revealed expression of Dll1 mRNA in transfected cells. B , Expression of Notch down-stream gene Hes1 was observed in transfected B16 tumor cells. C , Dll1 protein was detected by Western blot in B16 tumor cells. Data are reported as means±SD. **P

    Article Snippet: The primary antibodies included goat polyclonal IgG antibody to Dll1 and rabbit polyclonal IgG antibodies to Hes1, N-cadherin, or E-cadherin (Santa Cruz Biotechnology, USA). β-actin was detected simultaneously using 1:5000 dilution of monoclonal mouse anti-β-actin antibody (Sigma, USA) as a loading control.

    Techniques: Over Expression, Real-time Polymerase Chain Reaction, Expressing, Transfection, Western Blot

    Endothelial Dll1 regulates macrophage maturation and arteriogenesis. a Analysis of cell populations by flow cytometry in ischemic muscle of induced endothelial Dll1 mutant mice ( Dll1 iΔEC ) and control mice. n = 8 mice/group, error bars represent s.e.m. * p

    Journal: Nature Communications

    Article Title: Blood vessel control of macrophage maturation promotes arteriogenesis in ischemia

    doi: 10.1038/s41467-017-00953-2

    Figure Lengend Snippet: Endothelial Dll1 regulates macrophage maturation and arteriogenesis. a Analysis of cell populations by flow cytometry in ischemic muscle of induced endothelial Dll1 mutant mice ( Dll1 iΔEC ) and control mice. n = 8 mice/group, error bars represent s.e.m. * p

    Article Snippet: To address the role of endothelial Dll1 in macrophage maturation we pretreated EC with a DLL1 -specific siRNA, which efficiently reduced DLL1 expression without altering expression of other Notch ligands or Notch target genes (Supplementary Fig. ).

    Techniques: Flow Cytometry, Cytometry, Mutagenesis, Mouse Assay

    Ischemia triggers induction of Notch ligand Dll1 in arteries. a Quantitative RT-PCR analysis of cell populations of Cx3cr1 GFP/+ mice sorted from d3 ischemic muscle relative to bone marrow (BM) Ly6C hi monocytes. n = 3 independent experiments, error bars represent s.e.m. *** p

    Journal: Nature Communications

    Article Title: Blood vessel control of macrophage maturation promotes arteriogenesis in ischemia

    doi: 10.1038/s41467-017-00953-2

    Figure Lengend Snippet: Ischemia triggers induction of Notch ligand Dll1 in arteries. a Quantitative RT-PCR analysis of cell populations of Cx3cr1 GFP/+ mice sorted from d3 ischemic muscle relative to bone marrow (BM) Ly6C hi monocytes. n = 3 independent experiments, error bars represent s.e.m. *** p

    Article Snippet: To address the role of endothelial Dll1 in macrophage maturation we pretreated EC with a DLL1 -specific siRNA, which efficiently reduced DLL1 expression without altering expression of other Notch ligands or Notch target genes (Supplementary Fig. ).

    Techniques: Quantitative RT-PCR, Mouse Assay

    Endothelial Dll1 instructs macrophage maturation in vitro. a Quantitative RT-PCR analysis of p3 primary human aortic EC (HAEC, A) or coronary microvascular (MV) EC. n = 3 independent samples in duplicates/group, error bars represent s.e.m., ** p

    Journal: Nature Communications

    Article Title: Blood vessel control of macrophage maturation promotes arteriogenesis in ischemia

    doi: 10.1038/s41467-017-00953-2

    Figure Lengend Snippet: Endothelial Dll1 instructs macrophage maturation in vitro. a Quantitative RT-PCR analysis of p3 primary human aortic EC (HAEC, A) or coronary microvascular (MV) EC. n = 3 independent samples in duplicates/group, error bars represent s.e.m., ** p

    Article Snippet: To address the role of endothelial Dll1 in macrophage maturation we pretreated EC with a DLL1 -specific siRNA, which efficiently reduced DLL1 expression without altering expression of other Notch ligands or Notch target genes (Supplementary Fig. ).

    Techniques: In Vitro, Quantitative RT-PCR

    DLL1 instruct macrophage maturation in vitro. a Flow cytometry of Ly6C hi monocytes from Cx3cr1 GFP/+ mice cultured for 3d on IgG-Fc (con) or DLL1-Fc chimeric proteins, in the presence of 10 ng/ml CSF1. Representative of n = 5 independent experiments. b Quantitative RT-PCR analysis at d3 after culture with CSF1, CSF2. n = 2 independent experiments performed in duplicates, error bars represent s.e.m. ** p

    Journal: Nature Communications

    Article Title: Blood vessel control of macrophage maturation promotes arteriogenesis in ischemia

    doi: 10.1038/s41467-017-00953-2

    Figure Lengend Snippet: DLL1 instruct macrophage maturation in vitro. a Flow cytometry of Ly6C hi monocytes from Cx3cr1 GFP/+ mice cultured for 3d on IgG-Fc (con) or DLL1-Fc chimeric proteins, in the presence of 10 ng/ml CSF1. Representative of n = 5 independent experiments. b Quantitative RT-PCR analysis at d3 after culture with CSF1, CSF2. n = 2 independent experiments performed in duplicates, error bars represent s.e.m. ** p

    Article Snippet: To address the role of endothelial Dll1 in macrophage maturation we pretreated EC with a DLL1 -specific siRNA, which efficiently reduced DLL1 expression without altering expression of other Notch ligands or Notch target genes (Supplementary Fig. ).

    Techniques: In Vitro, Flow Cytometry, Cytometry, Mouse Assay, Cell Culture, Quantitative RT-PCR