dithiothreitol Millipore Search Results


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  • 95
    Millipore dl dithiothreitol solution
    Dl Dithiothreitol Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher dithiothreitol
    The effect of additives on preamplification specificity and efficiency. The yield and specificity of preamplification were evaluated at 35 conditions using 18 different additives. Analysis of preamplification: (A, B) preamplification response curves and corresponding melting curves for reactions in the presence of 1-µg/µl bovine serum albumin with 2.5% glycerol or water. (C) Average Cq ± SD for positive (n = 3) and negative samples (n = 3) applying different conditions for preamplification. ΔCq refers to the difference in Cq-values between positive and negative samples for each condition. Conditions are sorted according to ΔCq-value. (F) High-throughput qPCR data of individual assays for nine selected conditions. Average Cq ± SD (n = 3) is shown. The right y-axis indicates the percentage of negative controls positive for nonspecific PCR product formation, calculated from the 91 assays performing accurately in the preamplification (n total = 273, 3 negative qPCR controls per assay). 7-deaza-dGTP: 7-deaza-2′-deoxyguanosine 5′-triphosphate lithium salt; BSA: Bovine serum albumin; DMSO: Dimethyl sulfoxide; DTT: <t>Dithiothreitol;</t> LPA: GenElute-LPA; NTC: Non-template control; Poly(I:C): Polyinosinic–polycytidylic acid potassium salt; TMA Cl: Tetramethylammonium chloride.
    Dithiothreitol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 10368 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore dithiothreitol sputolysin
    Phenotypes of the Cn mon1 Δ mutant following exposure to various stresses. Spot dilution assays with WT (H99), Cn cna1 Δ (KK1), Cn mon1 Δ (HP55 and HP56), and Cn mon1 + Cn MON1 (HPC3 and HPC4). Cells were incubated overnight, diluted 10-fold, and plated on YPD agar. Plated cells were incubated for 2 days at 30, 37, 38, and 39 °C. (A) Serially diluted cells were also plated on YPD agar without or with (B) <t>dithiothreitol</t> (DTT), (C) sodium dodecylsulfate (SDS), (D) calcofluor white, (E) congo red, and (F) fluconazole at the indicated concentrations. Results shown are representative of two independent experiments.
    Dithiothreitol Sputolysin, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore sputolysin reagent
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Sputolysin Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore sputolysin solution
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Sputolysin Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Merck KGaA dithiothreitol
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Dithiothreitol, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 96/100, based on 194 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore cleland s reductacryl reagent
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Cleland S Reductacryl Reagent, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Millipore 100um dithiothreitol
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    100um Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore mℳ dithiothreitol
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Mℳ Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore dl dithiothreitol d10
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Dl Dithiothreitol D10, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Merck KGaA dithiothreitol dtt
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Dithiothreitol Dtt, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 95/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore dithiothreitol dtt
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Dithiothreitol Dtt, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore sputalysin
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Sputalysin, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Roche dithiothreitol
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Dithiothreitol, supplied by Roche, used in various techniques. Bioz Stars score: 98/100, based on 8639 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    75
    Millipore dithiothreitol iodoacetamide
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Dithiothreitol Iodoacetamide, supplied by Millipore, used in various techniques. Bioz Stars score: 75/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore papain dithiothreitol
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Papain Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore 50ul dithiothreitol dtt
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    50ul Dithiothreitol Dtt, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Millipore 1m dithiothreitol
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    1m Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore prepared dithiothreitol
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Prepared Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore 10 50 mm dithiothreitol
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    10 50 Mm Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Millipore rnase free dithiothreitol
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Rnase Free Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 82/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore dithiothreitol buffer
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Dithiothreitol Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore molecular grade dithiothreitol
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    Molecular Grade Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA 1 4 dithiothreitol
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    1 4 Dithiothreitol, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore 100 mm dithiothreitol
    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with <t>sputolysin</t> and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was
    100 Mm Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore m oxidized dithiothreitol
    Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) <t>dithiothreitol.</t> Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.
    M Oxidized Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Millipore dithiothreitol activated papain
    Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) <t>dithiothreitol.</t> Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.
    Dithiothreitol Activated Papain, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore heavy metal free dithiothreitol dtt
    Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) <t>dithiothreitol.</t> Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.
    Heavy Metal Free Dithiothreitol Dtt, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore phenylmethylsulfonyl fluoride pmsf dithiothreitol dithiothreitol dtt
    Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) <t>dithiothreitol.</t> Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.
    Phenylmethylsulfonyl Fluoride Pmsf Dithiothreitol Dithiothreitol Dtt, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 6 article reviews
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    phenylmethylsulfonyl fluoride pmsf dithiothreitol dithiothreitol dtt - by Bioz Stars, 2020-02
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    88
    Millipore alexa fluor594 cadaverine dithiothreitol
    Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) <t>dithiothreitol.</t> Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.
    Alexa Fluor594 Cadaverine Dithiothreitol, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor594 cadaverine dithiothreitol/product/Millipore
    Average 88 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    alexa fluor594 cadaverine dithiothreitol - by Bioz Stars, 2020-02
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    The effect of additives on preamplification specificity and efficiency. The yield and specificity of preamplification were evaluated at 35 conditions using 18 different additives. Analysis of preamplification: (A, B) preamplification response curves and corresponding melting curves for reactions in the presence of 1-µg/µl bovine serum albumin with 2.5% glycerol or water. (C) Average Cq ± SD for positive (n = 3) and negative samples (n = 3) applying different conditions for preamplification. ΔCq refers to the difference in Cq-values between positive and negative samples for each condition. Conditions are sorted according to ΔCq-value. (F) High-throughput qPCR data of individual assays for nine selected conditions. Average Cq ± SD (n = 3) is shown. The right y-axis indicates the percentage of negative controls positive for nonspecific PCR product formation, calculated from the 91 assays performing accurately in the preamplification (n total = 273, 3 negative qPCR controls per assay). 7-deaza-dGTP: 7-deaza-2′-deoxyguanosine 5′-triphosphate lithium salt; BSA: Bovine serum albumin; DMSO: Dimethyl sulfoxide; DTT: Dithiothreitol; LPA: GenElute-LPA; NTC: Non-template control; Poly(I:C): Polyinosinic–polycytidylic acid potassium salt; TMA Cl: Tetramethylammonium chloride.

    Journal: Expert Review of Molecular Diagnostics

    Article Title: Properties of targeted preamplification in DNA and cDNA quantification

    doi: 10.1586/14737159.2015.1057124

    Figure Lengend Snippet: The effect of additives on preamplification specificity and efficiency. The yield and specificity of preamplification were evaluated at 35 conditions using 18 different additives. Analysis of preamplification: (A, B) preamplification response curves and corresponding melting curves for reactions in the presence of 1-µg/µl bovine serum albumin with 2.5% glycerol or water. (C) Average Cq ± SD for positive (n = 3) and negative samples (n = 3) applying different conditions for preamplification. ΔCq refers to the difference in Cq-values between positive and negative samples for each condition. Conditions are sorted according to ΔCq-value. (F) High-throughput qPCR data of individual assays for nine selected conditions. Average Cq ± SD (n = 3) is shown. The right y-axis indicates the percentage of negative controls positive for nonspecific PCR product formation, calculated from the 91 assays performing accurately in the preamplification (n total = 273, 3 negative qPCR controls per assay). 7-deaza-dGTP: 7-deaza-2′-deoxyguanosine 5′-triphosphate lithium salt; BSA: Bovine serum albumin; DMSO: Dimethyl sulfoxide; DTT: Dithiothreitol; LPA: GenElute-LPA; NTC: Non-template control; Poly(I:C): Polyinosinic–polycytidylic acid potassium salt; TMA Cl: Tetramethylammonium chloride.

    Article Snippet: Preamplification additives The effects of 18 different PCR additives on the preamplification reaction were evaluated (final tested concentrations are shown): 7-deaza-2′-deoxyguanosine 5′-triphosphate lithium salt (50 and 100 µM; Sigma-Aldrich), ammonium sulfate (10 and 15 mM; Sigma-Aldrich), betaine (0.25 and 0.50 M; Sigma-Aldrich), bovine serum albumin supplied at 20 mg/ml in 10 mM Tris-HCl, 100 mM KCl, 1 mM EDTA and 50% glycerol (1 and 2 µg/µl; Thermo Scientific), D-(+)-trehalose dehydrate (0.15 and 0.30 M; Sigma-Aldrich), dimethyl sulfoxide (1%; Sigma-Aldrich), dithiothreitol (1.5 and 3.0 mM; Life Technologies), formamide (0.5 M; Sigma-Aldrich), gelatin (0.01 and 0.10%; Sigma-Aldrich), glycerol (2.5 and 5.0%; Sigma-Aldrich), IGEPAL CA-630 (0.25 and 0.50%, Sigma-Aldrich), l -carnitine inner salt (0.25 and 0.50 M; Sigma-Aldrich), GenElute-LPA (50 and 100 ng/µl; Sigma-Aldrich), polyinosinic:polycytidylic acid potassium salt (5 and 50 ng/µl; Sigma-Aldrich), tetramethylammonium chloride (30 and 60 mM; Sigma-Aldrich), Triton X-100 (0.2 and 0.4%; Sigma-Aldrich), TWEEN 20 (0.10 and 0.50%, Sigma-Aldrich) and yeast tRNA (50 and 100 ng/µl, Life Technologies).

    Techniques: High Throughput Screening Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    Phenotypes of the Cn mon1 Δ mutant following exposure to various stresses. Spot dilution assays with WT (H99), Cn cna1 Δ (KK1), Cn mon1 Δ (HP55 and HP56), and Cn mon1 + Cn MON1 (HPC3 and HPC4). Cells were incubated overnight, diluted 10-fold, and plated on YPD agar. Plated cells were incubated for 2 days at 30, 37, 38, and 39 °C. (A) Serially diluted cells were also plated on YPD agar without or with (B) dithiothreitol (DTT), (C) sodium dodecylsulfate (SDS), (D) calcofluor white, (E) congo red, and (F) fluconazole at the indicated concentrations. Results shown are representative of two independent experiments.

    Journal: Mycobiology

    Article Title: Mon1 Is Essential for Fungal Virulence and Stress Survival in Cryptococcus neoformans

    doi: 10.1080/12298093.2018.1468053

    Figure Lengend Snippet: Phenotypes of the Cn mon1 Δ mutant following exposure to various stresses. Spot dilution assays with WT (H99), Cn cna1 Δ (KK1), Cn mon1 Δ (HP55 and HP56), and Cn mon1 + Cn MON1 (HPC3 and HPC4). Cells were incubated overnight, diluted 10-fold, and plated on YPD agar. Plated cells were incubated for 2 days at 30, 37, 38, and 39 °C. (A) Serially diluted cells were also plated on YPD agar without or with (B) dithiothreitol (DTT), (C) sodium dodecylsulfate (SDS), (D) calcofluor white, (E) congo red, and (F) fluconazole at the indicated concentrations. Results shown are representative of two independent experiments.

    Article Snippet: To examine stress tolerance, each strain was serially diluted 10-fold and spotted onto YPD agar supplemented with the following compounds: dithiothreitol (DTT; Sigma), sodium dodecyl sulfate (SDS; Fisher, Fair Lawn, NJ, USA), and fluconazole (Sigma).

    Techniques: Mutagenesis, Incubation

    Protein solubility of ( A ) raw and ( B ) cooked noodles with 0%, 0.5%, 1.0%, 1.5% and 2.0% transglutaminase in extracting reagents phosphate buffer (PB; 0.1 M; pH 6.8), 8 M urea in PB (PB + Urea), 0.05 M dithiothreitol in PB (PB + DTT) and 0.05 M sodium dodecyl sulphate in PB (PB + SDS); * indicates significant difference ( p

    Journal: Foods

    Article Title: Effects of Transglutaminase on the Protein Network and In Vitro Starch Digestibility of Asian Wheat Noodles

    doi: 10.3390/foods8120607

    Figure Lengend Snippet: Protein solubility of ( A ) raw and ( B ) cooked noodles with 0%, 0.5%, 1.0%, 1.5% and 2.0% transglutaminase in extracting reagents phosphate buffer (PB; 0.1 M; pH 6.8), 8 M urea in PB (PB + Urea), 0.05 M dithiothreitol in PB (PB + DTT) and 0.05 M sodium dodecyl sulphate in PB (PB + SDS); * indicates significant difference ( p

    Article Snippet: Other extracting reagents were prepared by dissolving either urea (8 M; PB + Urea; Sigma-Aldrich, Saint Louis, MO, USA) or DL-dithiothreitol (0.05 M; PB + DTT; Sigma-Aldrich, Saint Louis, MO, USA) in 0.1 M phosphate buffer.

    Techniques: Solubility

    Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with sputolysin and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was

    Journal: Infection and Immunity

    Article Title: Expression and Antimicrobial Function of Bactericidal Permeability-Increasing Protein in Cystic Fibrosis Patients

    doi: 10.1128/IAI.02066-05

    Figure Lengend Snippet: Positive correlation of BPI and IL-8 concentrations in cell-free lung fluid from CF patients. Supernatants of sputum samples from CF patients were collected after treatment with sputolysin and subsequent centrifugation. IL-8 and BPI levels were determined by ELISA. Linear regression shows the positive correlation r 2 = 0.6785. The P value was

    Article Snippet: To extract the cells in sputum, fresh sputum samples were treated with 2 ml of sputolysin reagent (Calbiochem; Merck, Darmstadt, Germany) and 18 ml of phosphate-buffered saline (PBS) for 15 min at room temperature (RT) with occasional mixing.

    Techniques: Centrifugation, Enzyme-linked Immunosorbent Assay

    Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) dithiothreitol. Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Redox Regulation of Methionine Aminopeptidase 2 Activity *

    doi: 10.1074/jbc.M114.554253

    Figure Lengend Snippet: Redox potential of the MetAP2 disulfide bond. A , the labeling of fully reduced MetAP2 by the biotin-linked maleimide, MPB, was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (DTT red ) to oxidized (DTT ox ) dithiothreitol. Biotin incorporation was measured by SDS-PAGE and blotting with streptavidin-peroxidase. B , plot of the ratio of reduced to oxidized MetAP2 as a function of the ratio of reduced to oxidized DTT. The solid line . The calculated equilibrium constant is 0.028 (95% confidence interval: 0.012–0.044). From the Nernst equation, the standard redox potential of the Cys 228 -Cys 448 disulfide is −261 mV. Data points and error bars , mean ± S.E. of three independent experiments. C , the initial rate of hydrolysis of Met-Gly-Pro-AMC by fully oxidized or reduced MetAP2 was used to calculate the ratio of reduced to oxidized enzyme as a function of the ratio of reduced (Trx red ) to oxidized (Trx ox ) thioredoxin. The solid line . The calculated equilibrium constant is 0.740 (95% confidence interval: 0.600–0.688), which equates to a standard redox potential of −266 mV for the Cys 228 -Cys 448 disulfide bond. Data points and error bars , mean ± S.E. of three independent experiments.

    Article Snippet: The blocked MetAP2 (250 n m ) was incubated with 1 m m oxidized dithiothreitol (DTTox , trans -4,5-dihydroxy-1,2-dithiane; Sigma) and various concentrations of reduced dithiothreitol (DTTred ) for 18 h at 30 °C in the phosphate buffer.

    Techniques: Labeling, SDS Page