Journal: ACS Omega

Article Title: Identification of Bithionol, Dichlorophen, and Miconazole as Antibacterial Agents against Acinetobacter calcoaceticus

doi: 10.1021/acsomega.0c03211

Figure Lengend Snippet: Drug screening against A. calcoaceticus . Zones of inhibition of A. calcoaceticus growth by 1 μL of 3.3 mM Miconazole, Dichlorophen, Doxycycline (positive control, halo circled with the dotted line for reference), and DMSO (negative control) on lawns of 75 μL of A. calcoaceticus culture incubated overnight at 37 °C. Normalized fold changes of the halos are shown at the bottom right corner of the images.

Article Snippet: Dichlorophen structural analogues, Bithionol and Triclosan, were obtained from Fluka Analytical and Alfa Aesar, respectively.

Techniques: Drug discovery, Inhibition, Positive Control, Negative Control, Incubation

Journal: ACS Omega

Article Title: Identification of Bithionol, Dichlorophen, and Miconazole as Antibacterial Agents against Acinetobacter calcoaceticus

doi: 10.1021/acsomega.0c03211

Figure Lengend Snippet: Diffusion susceptibility test on A. calcoaceticus . Representative zones of inhibition of A. calcoaceticus growth by 1 μL of MIC and sub-MIC of Miconazole, Dichlorophen, Bithionol, and Triclosan. DMSO serves as the negative control. MIC and sub-MIC values are indicated above corresponding halo images. Normalized fold changes of the halos are shown at the bottom right corner of the images.

Article Snippet: Dichlorophen structural analogues, Bithionol and Triclosan, were obtained from Fluka Analytical and Alfa Aesar, respectively.

Techniques: Diffusion-based Assay, Inhibition, Negative Control

Journal: ACS Omega

Article Title: Identification of Bithionol, Dichlorophen, and Miconazole as Antibacterial Agents against Acinetobacter calcoaceticus

doi: 10.1021/acsomega.0c03211

Figure Lengend Snippet: Kinetic growth of A. calcoaceticus with varying concentrations of hits. Antimicrobial activity of Miconazole, Dichlorophen, Bithionol, and Triclosan at concentrations ranging from 32 to 1 μM on A. calcoaceticus growth.

Article Snippet: Dichlorophen structural analogues, Bithionol and Triclosan, were obtained from Fluka Analytical and Alfa Aesar, respectively.

Techniques: Activity Assay

Journal: ACS Omega

Article Title: Identification of Bithionol, Dichlorophen, and Miconazole as Antibacterial Agents against Acinetobacter calcoaceticus

doi: 10.1021/acsomega.0c03211

Figure Lengend Snippet: Broad-spectrum antibacterial activity of hits. Zones of inhibition by our hits on SMR A. calcoaceticus , E. coli , C. freundii , E. aerogenes , B. cereus , and B. subtilis . Normalized fold changes are indicated at the bottom right corner of images. E. aerogenes was insensitive to Miconazole, Dichlorophen, and Bithionol.

Article Snippet: Dichlorophen structural analogues, Bithionol and Triclosan, were obtained from Fluka Analytical and Alfa Aesar, respectively.

Techniques: Activity Assay, Inhibition

Journal: ACS Omega

Article Title: Identification of Bithionol, Dichlorophen, and Miconazole as Antibacterial Agents against Acinetobacter calcoaceticus

doi: 10.1021/acsomega.0c03211

Figure Lengend Snippet: Broad-spectrum antibacterial activity of hits. Minimum inhibitory concentrations (MICs) and standard deviations of Miconazole, Dichlorophen, Bithionol, and Triclosan versus select bacterial strains on solid growth medium.

Article Snippet: Dichlorophen structural analogues, Bithionol and Triclosan, were obtained from Fluka Analytical and Alfa Aesar, respectively.

Techniques: Activity Assay

Journal: ACS Omega

Article Title: Identification of Bithionol, Dichlorophen, and Miconazole as Antibacterial Agents against Acinetobacter calcoaceticus

doi: 10.1021/acsomega.0c03211

Figure Lengend Snippet: Broad-spectrum antibacterial activity of combinations of hits. Images depict zones of inhibition of B. subtilis , E. coli , C. freundii , B. cereus , A. calcoaceticus , and SMR A. calcoaceticus treated with Miconazole, Dichlorophen, Bithionol, and Triclosan combinations at concentrations shown in Table . DMSO serves as the negative control. Normalized fold changes are shown on the bottom right corner of each image.

Article Snippet: Dichlorophen structural analogues, Bithionol and Triclosan, were obtained from Fluka Analytical and Alfa Aesar, respectively.

Techniques: Activity Assay, Inhibition, Negative Control

Journal: ACS Omega

Article Title: Identification of Bithionol, Dichlorophen, and Miconazole as Antibacterial Agents against Acinetobacter calcoaceticus

doi: 10.1021/acsomega.0c03211

Figure Lengend Snippet: Broad-Spectrum Antimicrobial Activity of Hits and Analogues as Assessed by MIC on Solid Growth Medium

Article Snippet: Dichlorophen structural analogues, Bithionol and Triclosan, were obtained from Fluka Analytical and Alfa Aesar, respectively.

Techniques: Activity Assay, Analogues, Bacteria, Standard Deviation

Journal: ACS Omega

Article Title: Identification of Bithionol, Dichlorophen, and Miconazole as Antibacterial Agents against Acinetobacter calcoaceticus

doi: 10.1021/acsomega.0c03211

Figure Lengend Snippet: Concentrations of Drugs Used in Broad-Spectrum Analysis of Hit Combination of Hits a

Article Snippet: Dichlorophen structural analogues, Bithionol and Triclosan, were obtained from Fluka Analytical and Alfa Aesar, respectively.

Techniques: Bacteria