Journal: Clinical hemorheology and microcirculation

Article Title: Effects of ethanol on red blood cell rheological behavior

doi: 10.3233/CH-2012-1632

Figure Lengend Snippet: Morphological appearance of erythrocytes visualized by DIC light microscopy. A: Untreated cells in PBS. B: Erythrocytes in PBS with 2% ethanol concentration. C: RBC in the LORCA viscous medium (dextran). D: Erythrocytes in dextran containing 2% ethanol.

Article Snippet: RBC shape was evaluated by DIC light microscopy (model BX50F; Olympus, Tokyo, Japan).

Techniques: Light Microscopy, Concentration Assay

Journal: Scientific Reports

Article Title: Nuclear expansion and pore opening are instant signs of neuronal hypoxia and can identify poorly fixed brains

doi: 10.1038/s41598-018-32878-1

Figure Lengend Snippet: Dysmorphic neurons surrounded by swollen astrocyte end-feet displayed a donut-like staining with NeuN. Unlike well-perfused Nissl-stained paraffin-embedded brain sections ( a ), swollen astrocyte end-feet (AEF) surrounding the soma of dysmorphic neurons were observed in poorly-perfused sections (arrows in b ) as typically seen after hypoxia/ischemia. DIC images ( c and d ) showed that, in contrast to granular surface corresponding to cell bodies and capillaries in well-perfused sections ( c ), a striated tissue surface as a common type of artifact was noted in poorly-perfused cortical sections consistent with cellular edema ( d ). NeuN immunostaining of the frozen brain sections confirmed that the dysmorphic cells were neurons (arrowheads in f ) surrounded by swollen cellular processes (AEF) as illustrated by phase contrast imaging of the same section (low contrast rim - arrowheads in e ). Intriguingly, these dysmorphic neurons displayed a circular NeuN staining pattern (donut-like stainings - arrowhaeds in f ), leaving the center part of the nucleus unstained. Intact neurons in poorly perfused sections exhibited a homogenous NeuN labeling and did not show the pericellular low contrast rim in phase contrast microscopy (arrow in e and f ). Nissl-stained and DIC images were taken from cortical areas 4 ( * ). Scale bars: 25 µm.

Article Snippet: Representative sections were also imaged under laser scanning confocal microscope (Carl Zeiss LSM 510 and LSM 880 system equipped with fast airy scan detector, or Leica SP8) and with differential interference microscopy (DIC) (Carl Zeiss Axioimager M1).

Techniques: Staining, Immunostaining, Imaging, Labeling, Microscopy