Journal: ACS chemical biology

Article Title: A Bacterial Cell Shape-Determining Inhibitor

doi: 10.1021/acschembio.5b01039

Figure Lengend Snippet: C. jejuni wild type 81–176 and ΔkpsM show cell straightening in the presence of inhibitor 1. DIC microscopy images of C. jejuni 81–176 (A) and the acapsular ΔkpsM (D) without inhibitor (left) and treated with 2.3 mM inhibitor for 24h (right). Scatter plots arraying cell length (x-axis, µm) and side curvature (y-axis, arbitrary units) for 81–176 (B) and ΔkpsM (E) grown for 24 h with and without inhibitor 1. Each contour represents the morphology of a single cell from a 1000× DIC image as determined using CellTool software. Smooth histograms displaying population side curvature (x-axis, arbitrary units) as a density function (y-axis) for 81–176 (C) and ΔkpsM (F) grown for 24 h with and without inhibitor 1. Kolmogorov–Smirnov statistical comparisons of population cell side curvature distributions indicate that treated cells have significantly lower side curvatures than untreated cells (81–176, p<0.00001; ΔkpsM, p<0.00001, with p<0.05 indicating significance).

Article Snippet: Cell morphology was monitored by DIC microscopy (described below) over 24 h. At 24 h, changes in cell morphology were quantified by CellTool analysis on images from DIC microscopy (described below).

Techniques: Microscopy, Software