diaminobenzidine Search Results


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  • 99
    Vector Laboratories dab peroxidase hrp substrate kit with nickel 3 3 diaminobenzidine
    Dab Peroxidase Hrp Substrate Kit With Nickel 3 3 Diaminobenzidine, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 492 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore 3 3 diaminobenzidine
    3 3 Diaminobenzidine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 15839 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Agilent technologies diaminobenzidine
    (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of <t>diaminobenzidine-stained</t> cells (brown areas) around the peri-implant bone where the biomarker RUNX-2 were intense, represented by black arrow, denoting a greater active of the osteoblastogenesis; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing the expression of RUNX-2 among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri- implant bone healing from RLX group showing a moderate labeling for 14 days and middle labeling for 42 days of the biomarker RUNX-2 represented by red arrows
    Diaminobenzidine, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 11905 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore 3 3 diaminobenzidine tetrahydrochloride
    (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of <t>diaminobenzidine-stained</t> cells (brown areas) around the peri-implant bone where the biomarker RUNX-2 were intense, represented by black arrow, denoting a greater active of the osteoblastogenesis; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing the expression of RUNX-2 among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri- implant bone healing from RLX group showing a moderate labeling for 14 days and middle labeling for 42 days of the biomarker RUNX-2 represented by red arrows
    3 3 Diaminobenzidine Tetrahydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7492 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 3 diaminobenzidine tetrahydrochloride/product/Millipore
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    96
    Millipore diaminobenzidine
    (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of <t>diaminobenzidine-stained</t> cells (brown areas) around the peri-implant bone where the biomarker RUNX-2 were intense, represented by black arrow, denoting a greater active of the osteoblastogenesis; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing the expression of RUNX-2 among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri- implant bone healing from RLX group showing a moderate labeling for 14 days and middle labeling for 42 days of the biomarker RUNX-2 represented by red arrows
    Diaminobenzidine, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 14403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies 3 3 diaminobenzidine tetrahydrochloride
    Representative images of immunohistochemical staining, developed with <t>3,3′-Diaminobenzidine</t> <t>tetrahydrochloride</t> and counter stained with hematoxylin for Col III in rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups, and (D) the statistical analysis. Scale bar represents 100 µm. *P
    3 3 Diaminobenzidine Tetrahydrochloride, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 2721 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher diaminobenzidine
    Meprin-α is expressed in cancer cells. Immunostaining for meprin-α on tissue sections from formalin-fixed and paraffin-embedded colorectal tissue samples using a rabbit polyclonal primary antibody together with a peroxidase-coupled secondary antibody and <t>diaminobenzidine</t> as chromogenic substrate. Counter stain: hematoxylin. Objective magnification: 40×. Bar = 50 µm. (A) In normal colon mucosa meprin-α is barely detectable. Occasionally, signals were detected at the base of crypt regions in the cytosol of cells with apically localized nuclei (asterisks). The specificity of these signals and the identity of the cells was not further investigated. (B) Representative sample of a meprin-α positive primary tumor stage III. Tumors exhibit a mosaic expression pattern. In cancer cell clusters, cells with strong signals for meprin-α (arrows) are intermixed with cells that show weak signals or no signal at all. Cells in the stroma do not express meprin-α (C) Representative sample of meprin-α positive primary tumor stage IV showing clusters of meprin-α positive cancer cells (arrows). (D) Cancer cells express meprin-α (arrows) in liver metastases (Met). The surrounding normal liver tissue (Hep) is only weakly stained.
    Diaminobenzidine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore 3 diaminobenzidine
    Meprin-α is expressed in cancer cells. Immunostaining for meprin-α on tissue sections from formalin-fixed and paraffin-embedded colorectal tissue samples using a rabbit polyclonal primary antibody together with a peroxidase-coupled secondary antibody and <t>diaminobenzidine</t> as chromogenic substrate. Counter stain: hematoxylin. Objective magnification: 40×. Bar = 50 µm. (A) In normal colon mucosa meprin-α is barely detectable. Occasionally, signals were detected at the base of crypt regions in the cytosol of cells with apically localized nuclei (asterisks). The specificity of these signals and the identity of the cells was not further investigated. (B) Representative sample of a meprin-α positive primary tumor stage III. Tumors exhibit a mosaic expression pattern. In cancer cell clusters, cells with strong signals for meprin-α (arrows) are intermixed with cells that show weak signals or no signal at all. Cells in the stroma do not express meprin-α (C) Representative sample of meprin-α positive primary tumor stage IV showing clusters of meprin-α positive cancer cells (arrows). (D) Cancer cells express meprin-α (arrows) in liver metastases (Met). The surrounding normal liver tissue (Hep) is only weakly stained.
    3 Diaminobenzidine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore diaminobenzidine tetrahydrochloride
    Meprin-α is expressed in cancer cells. Immunostaining for meprin-α on tissue sections from formalin-fixed and paraffin-embedded colorectal tissue samples using a rabbit polyclonal primary antibody together with a peroxidase-coupled secondary antibody and <t>diaminobenzidine</t> as chromogenic substrate. Counter stain: hematoxylin. Objective magnification: 40×. Bar = 50 µm. (A) In normal colon mucosa meprin-α is barely detectable. Occasionally, signals were detected at the base of crypt regions in the cytosol of cells with apically localized nuclei (asterisks). The specificity of these signals and the identity of the cells was not further investigated. (B) Representative sample of a meprin-α positive primary tumor stage III. Tumors exhibit a mosaic expression pattern. In cancer cell clusters, cells with strong signals for meprin-α (arrows) are intermixed with cells that show weak signals or no signal at all. Cells in the stroma do not express meprin-α (C) Representative sample of meprin-α positive primary tumor stage IV showing clusters of meprin-α positive cancer cells (arrows). (D) Cancer cells express meprin-α (arrows) in liver metastases (Met). The surrounding normal liver tissue (Hep) is only weakly stained.
    Diaminobenzidine Tetrahydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2603 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies 3 3 diaminobenzidine dab
    Identifying <t>3,3′-diaminobenzidine</t> <t>(DAB)-stained</t> regions using the NanoSuit method. a – g Colon tissue immunostained using an antibody against smooth muscle actin with color development via DAB staining (brown). b Staining of the section shown in a with 1% gold (III) chloride. The DAB-staining intensity of the low-vacuum scanning electron microscopy (Lv-SEM) image was selectively enhanced as white color, taken in backscattered electron (BSE) mode. c Image of a control section without gold (III) chloride treatment. d DAB staining of the muscularis propria of a colon tissue section. e Lv-SEM image, taken in BSE mode. f DAB-stained section of a magnified area of the muscularis propria and blood vessels. g Three-dimensional (3D) structure of the correlative region shown in f . Lv-SEM image, taken in mixed BSE and SE mode. h DAB-stained dendritic cells (DCs) of the human epidermis. i Field emission-scanning electron microscopy (FE-SEM) image of DAB-stained DCs after treatment with 1% gold chloride, taken in yttrium–aluminum–garnet BSE mode. j 3D structure of DAB-stained DCs in an FE-SEM image, taken in secondary electron mode. The scale bars represent 2 mm ( a – c ), 500 ( d , e ), 50 ( f , g ), and 10 μm ( h – j )
    3 3 Diaminobenzidine Dab, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1470 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of diaminobenzidine-stained cells (brown areas) around the peri-implant bone where the biomarker RUNX-2 were intense, represented by black arrow, denoting a greater active of the osteoblastogenesis; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing the expression of RUNX-2 among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri- implant bone healing from RLX group showing a moderate labeling for 14 days and middle labeling for 42 days of the biomarker RUNX-2 represented by red arrows

    Journal: Journal of Applied Oral Science

    Article Title: A SERM increasing the expression of the osteoblastogenesis and mineralization-related proteins and improving quality of bone tissue in an experimental model of osteoporosis

    doi: 10.1590/1678-7757-2017-0329

    Figure Lengend Snippet: (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of diaminobenzidine-stained cells (brown areas) around the peri-implant bone where the biomarker RUNX-2 were intense, represented by black arrow, denoting a greater active of the osteoblastogenesis; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing the expression of RUNX-2 among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri- implant bone healing from RLX group showing a moderate labeling for 14 days and middle labeling for 42 days of the biomarker RUNX-2 represented by red arrows

    Article Snippet: Diaminobenzidine (Dako, Glostrup, Denmark) was used as the chromogen.

    Techniques: Staining, Biomarker Assay, Expressing, Labeling

    (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of diaminobenzidine-stained cells (brown areas) around the peri-implant bone where the biomarker β-catenin were intense, represented by black arrows, denoting an improvement in the bone formation; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing the expression of β-catenin among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri-implant bone healing from RLX group showing a moderate labeling of the biomarker β-catenin represented by red arrows

    Journal: Journal of Applied Oral Science

    Article Title: A SERM increasing the expression of the osteoblastogenesis and mineralization-related proteins and improving quality of bone tissue in an experimental model of osteoporosis

    doi: 10.1590/1678-7757-2017-0329

    Figure Lengend Snippet: (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of diaminobenzidine-stained cells (brown areas) around the peri-implant bone where the biomarker β-catenin were intense, represented by black arrows, denoting an improvement in the bone formation; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing the expression of β-catenin among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri-implant bone healing from RLX group showing a moderate labeling of the biomarker β-catenin represented by red arrows

    Article Snippet: Diaminobenzidine (Dako, Glostrup, Denmark) was used as the chromogen.

    Techniques: Staining, Biomarker Assay, Expressing, Labeling

    (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of diaminobenzidine-stained cells (brown areas) around the peri-implant bone where the biomarker osteocalcin were intense, represented by black arrows, denoting an improvement in the bone mineralization; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing the expression of osteocalcin among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri- implant bone healing from RLX group showing a moderate labeling of the biomarker osteocalcin represented by red arrows

    Journal: Journal of Applied Oral Science

    Article Title: A SERM increasing the expression of the osteoblastogenesis and mineralization-related proteins and improving quality of bone tissue in an experimental model of osteoporosis

    doi: 10.1590/1678-7757-2017-0329

    Figure Lengend Snippet: (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of diaminobenzidine-stained cells (brown areas) around the peri-implant bone where the biomarker osteocalcin were intense, represented by black arrows, denoting an improvement in the bone mineralization; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing the expression of osteocalcin among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri- implant bone healing from RLX group showing a moderate labeling of the biomarker osteocalcin represented by red arrows

    Article Snippet: Diaminobenzidine (Dako, Glostrup, Denmark) was used as the chromogen.

    Techniques: Staining, Biomarker Assay, Expressing, Labeling

    (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of diaminobenzidine-stained cells (brown areas) around the peri-implant bone where the biomarker osteopontin were intense, represented by black arrows, denoting an improvement in the bone mineralization; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing expression of osteopontin among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri- implant bone healing from RLX group showing a moderate labeling of the biomarker osteopontin represented by red arrows

    Journal: Journal of Applied Oral Science

    Article Title: A SERM increasing the expression of the osteoblastogenesis and mineralization-related proteins and improving quality of bone tissue in an experimental model of osteoporosis

    doi: 10.1590/1678-7757-2017-0329

    Figure Lengend Snippet: (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of diaminobenzidine-stained cells (brown areas) around the peri-implant bone where the biomarker osteopontin were intense, represented by black arrows, denoting an improvement in the bone mineralization; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing expression of osteopontin among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri- implant bone healing from RLX group showing a moderate labeling of the biomarker osteopontin represented by red arrows

    Article Snippet: Diaminobenzidine (Dako, Glostrup, Denmark) was used as the chromogen.

    Techniques: Staining, Biomarker Assay, Expressing, Labeling

    (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of diaminobenzidine-stained cells (brown areas) around the peri-implant bone where the biomarker WNT were intense, represented by black arrows, denoting an improvement in the bone formation; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing the expression of WNT among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri-implant bone healing from RLX group showing an intense labeling of WNT pathway represented by red arrows

    Journal: Journal of Applied Oral Science

    Article Title: A SERM increasing the expression of the osteoblastogenesis and mineralization-related proteins and improving quality of bone tissue in an experimental model of osteoporosis

    doi: 10.1590/1678-7757-2017-0329

    Figure Lengend Snippet: (A) Photomicrographs in a higher original objective (x40) of the different groups (SHAM, OVX, and RLX) and periods (14 and 42 days), in which is possible to observe an increased area of diaminobenzidine-stained cells (brown areas) around the peri-implant bone where the biomarker WNT were intense, represented by black arrows, denoting an improvement in the bone formation; (B) The chart shows the scores submitted to the Kappa test, in which the index was adjusted to > 0.8, representing the expression of WNT among the groups and in both periods; (C and D) Photomicrographs in a higher original objective (x100) about the 14 and 42 days of peri-implant bone healing from RLX group showing an intense labeling of WNT pathway represented by red arrows

    Article Snippet: Diaminobenzidine (Dako, Glostrup, Denmark) was used as the chromogen.

    Techniques: Staining, Biomarker Assay, Expressing, Labeling

    Nphs2-, Nphs1- and Synpo staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.

    Journal: PLoS ONE

    Article Title: Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression

    doi: 10.1371/journal.pone.0137043

    Figure Lengend Snippet: Nphs2-, Nphs1- and Synpo staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Nphs2, Nphs1 and Synpo stainings result from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A+B) Nphs2 staining. (A) Tubb2b brdp/brdp kidneys show a specific Nphs2 staining in podocytes arranged like a row of pearls at the periphery of the glomerulus. (B) Within the developing wild type kidney early capillary loop stages and maturing glomeruli are labeled. (C+D) Nphs1 staining. (E+F) Synpo staining. (C-F) Both, Nphs1 and Synpo patterns are comparable to the Nphs2 staining. Black asterisks: Enlarged section areas on the right. Scale bars = 50μm.

    Article Snippet: After blocking of unspecific sites (0,1% Avidin, 0,01% Biotin and Protein Block Serum-free; all obtained from DAKO, Hamburg, Germany), sections were stained with the first antibody for 2 h diluted in Antibody Diluent with Background Reducing Components (DAKO, Hamburg, Germany) followed by incubation with the biotinylated secondary antibody (30 min; DAKO, Hamburg, Germany) and the streptavidin-peroxidase reagent (30 min, DAKO, Hamburg, Germany) and finally 3,3'-diaminobenzidine (DAB; DAKO, Hamburg, Germany) used as the chromogen.

    Techniques: Staining, Immunohistochemistry, Mouse Assay, Labeling

    TUBB2B and DCDC2 are expressed in human kidneys. (A) Western-blot analysis to measure the existence of TUBB2B and DCDC2 in human kidneys. GAPDH = loading control. CC = Podocyte cell culture. WT = Wild type kidney. (B+C) Immunohistochemical staining of TUBB2B and DCDC2 in healthy human kidneys. 3,3'-diaminobenzidine (DAB) was used as chromogen (brown staining) and nuclei were stained with hematoxylin (blue). The black arrows mark a nuclear as well as cytoplasmic staining of TUBB2B and a cytoplasmic staining of DCDC2 in podocytes. A negative control was performed without the primary antibody ( S1 Fig ). T = tubule. EPC = parietal epithelial cell. Scale bar = 50 μm.

    Journal: PLoS ONE

    Article Title: Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression

    doi: 10.1371/journal.pone.0137043

    Figure Lengend Snippet: TUBB2B and DCDC2 are expressed in human kidneys. (A) Western-blot analysis to measure the existence of TUBB2B and DCDC2 in human kidneys. GAPDH = loading control. CC = Podocyte cell culture. WT = Wild type kidney. (B+C) Immunohistochemical staining of TUBB2B and DCDC2 in healthy human kidneys. 3,3'-diaminobenzidine (DAB) was used as chromogen (brown staining) and nuclei were stained with hematoxylin (blue). The black arrows mark a nuclear as well as cytoplasmic staining of TUBB2B and a cytoplasmic staining of DCDC2 in podocytes. A negative control was performed without the primary antibody ( S1 Fig ). T = tubule. EPC = parietal epithelial cell. Scale bar = 50 μm.

    Article Snippet: After blocking of unspecific sites (0,1% Avidin, 0,01% Biotin and Protein Block Serum-free; all obtained from DAKO, Hamburg, Germany), sections were stained with the first antibody for 2 h diluted in Antibody Diluent with Background Reducing Components (DAKO, Hamburg, Germany) followed by incubation with the biotinylated secondary antibody (30 min; DAKO, Hamburg, Germany) and the streptavidin-peroxidase reagent (30 min, DAKO, Hamburg, Germany) and finally 3,3'-diaminobenzidine (DAB; DAKO, Hamburg, Germany) used as the chromogen.

    Techniques: Western Blot, Cell Culture, Immunohistochemistry, Staining, Negative Control

    Tubb2b staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Tubb2b staining results from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A) Tubb2b brdp/brdp kidneys show a specific cytoplasmic Tubb2b expression in tubuli (T) and in podocytes (P), confirming the developmental defects seen with the Wt1, Nphs2, Nphs1 and Synpo stainings. (B) Tubb2b expression in wild type kidneys is restricted to the mature podocytes. Interestingly in the developing wild type kidney Tubb2b is not expressed in the early developmental stages of maturing podocytes. Note, that in murine podocytes nuclear Tubb2b seems much less expressed compared to human kidneys. Black asterisks: Enlarged section areas on the right. Scale bars = 50 μm.

    Journal: PLoS ONE

    Article Title: Everolimus Stabilizes Podocyte Microtubules via Enhancing TUBB2B and DCDC2 Expression

    doi: 10.1371/journal.pone.0137043

    Figure Lengend Snippet: Tubb2b staining of wild type and Tubb2b brdp/brdp mouse kidneys (E18.5). Immunohistochemical staining of mice kidneys (dark-brown Tubb2b staining results from 3,3'-diaminobenzidine. Nuclei were stained with hematoxylin (blue). (A) Tubb2b brdp/brdp kidneys show a specific cytoplasmic Tubb2b expression in tubuli (T) and in podocytes (P), confirming the developmental defects seen with the Wt1, Nphs2, Nphs1 and Synpo stainings. (B) Tubb2b expression in wild type kidneys is restricted to the mature podocytes. Interestingly in the developing wild type kidney Tubb2b is not expressed in the early developmental stages of maturing podocytes. Note, that in murine podocytes nuclear Tubb2b seems much less expressed compared to human kidneys. Black asterisks: Enlarged section areas on the right. Scale bars = 50 μm.

    Article Snippet: After blocking of unspecific sites (0,1% Avidin, 0,01% Biotin and Protein Block Serum-free; all obtained from DAKO, Hamburg, Germany), sections were stained with the first antibody for 2 h diluted in Antibody Diluent with Background Reducing Components (DAKO, Hamburg, Germany) followed by incubation with the biotinylated secondary antibody (30 min; DAKO, Hamburg, Germany) and the streptavidin-peroxidase reagent (30 min, DAKO, Hamburg, Germany) and finally 3,3'-diaminobenzidine (DAB; DAKO, Hamburg, Germany) used as the chromogen.

    Techniques: Staining, Immunohistochemistry, Mouse Assay, Expressing

    Disclosing of the dental plaque by HRP. Dental plaques and biofilms by oral gram-positive bacteria (A) S. sanguinis and (B) S. salivarius , (C) the major gram-positive bacterium L. casei , and (D) the major gram-negative bacterium E. coli were developed on the artificial tooth surface. (E) The dental pellicle and (F) the naked artificial tooth surface (f) were additionally applied. They were subsequently stained by HRP with the chromogenic DAB substrate. (G) S. sanguinis and (H) S. salivarius , (I) L. casei , (J) E. coli and the (K) dental pellicle developed on surfaces of the artificial tooth, and (L) the naked artificial tooth surface was additionally stained by the commercially available ‘dental plaque disclosing agent’ D C Red No 28. The partial magnified images of the artificial tooth surfaces are shown in large windows, with the whole images shown in small windows at upper right. The long scales indicate 1 mm, whereas, the short scales in each of the small windows on the upper right indicate 10 mm. HRP, horseradish peroxidase; S. sanguinis, Streptococcus sanguinis; S. salivarius, Streptococcus salivarius; L. casei, Lactobacillus casei; E. coli, Escherichia coli ; DAB, diaminobenzidine.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Horseradish peroxidase interacts with the cell wall peptidoglycans on oral bacteria

    doi: 10.3892/etm.2020.9016

    Figure Lengend Snippet: Disclosing of the dental plaque by HRP. Dental plaques and biofilms by oral gram-positive bacteria (A) S. sanguinis and (B) S. salivarius , (C) the major gram-positive bacterium L. casei , and (D) the major gram-negative bacterium E. coli were developed on the artificial tooth surface. (E) The dental pellicle and (F) the naked artificial tooth surface (f) were additionally applied. They were subsequently stained by HRP with the chromogenic DAB substrate. (G) S. sanguinis and (H) S. salivarius , (I) L. casei , (J) E. coli and the (K) dental pellicle developed on surfaces of the artificial tooth, and (L) the naked artificial tooth surface was additionally stained by the commercially available ‘dental plaque disclosing agent’ D C Red No 28. The partial magnified images of the artificial tooth surfaces are shown in large windows, with the whole images shown in small windows at upper right. The long scales indicate 1 mm, whereas, the short scales in each of the small windows on the upper right indicate 10 mm. HRP, horseradish peroxidase; S. sanguinis, Streptococcus sanguinis; S. salivarius, Streptococcus salivarius; L. casei, Lactobacillus casei; E. coli, Escherichia coli ; DAB, diaminobenzidine.

    Article Snippet: Next, the dental plaque was disclosed with 10 mg/ml diaminobenzidine (DAB) (Dako), the chromogenic substrate, in 50 mM Tris-HCl (pH 7.6) supplemented with 7.5 µl/ml of 3% hydrogen peroxide for 5 min at RT.

    Techniques: Staining

    Immunoreactivity for GFAP and Iba-1. Representative images of GFAP + astrocytes and Iba-1 + microglia in the cortex and hippocampus of 5 month-old male F344 and HIV-1 Tg rats. 3,3-diaminobenzidine (brown) stained cells displayed normal process-bearing morphologies with no evidence of microglia activation or astrocyte hypertrophy. Quantitation of the % area occupied by Iba-1 + immunoreactive cells (mean +/− SD) in the cortex (n=6) and hippocampus (n=10) is provided within each representative image.

    Journal: Neurotoxicity research

    Article Title: Age-related decrease in tyrosine hydroxylase immunoreactivity in the substantia nigra and region-specific changes in microglia morphology in HIV-1 Tg rats

    doi: 10.1007/s12640-019-00077-z

    Figure Lengend Snippet: Immunoreactivity for GFAP and Iba-1. Representative images of GFAP + astrocytes and Iba-1 + microglia in the cortex and hippocampus of 5 month-old male F344 and HIV-1 Tg rats. 3,3-diaminobenzidine (brown) stained cells displayed normal process-bearing morphologies with no evidence of microglia activation or astrocyte hypertrophy. Quantitation of the % area occupied by Iba-1 + immunoreactive cells (mean +/− SD) in the cortex (n=6) and hippocampus (n=10) is provided within each representative image.

    Article Snippet: Rinsed sections were incubated using Vectastain Elite ABC kit for 90 min at RT, washed in 1xTBS and treated with 3-diaminobenzidine (DAB; Agilent Technologies, Santa Clara, CA) [10 mg DAB in 40 ml 1xTBS] and chromogen with nickel chloride amplification.

    Techniques: Staining, Activation Assay, Quantitation Assay

    Tyrosine Hydroxylase Immunoreactivity and Unbiased Stereology. A. Estimates of TH+ neuronal number between 2 and 8 months of age. Scatter graph of individual values (% of F344 control) at each age. Bar graphs of mean +/− SEM for 2–5 months (n=9) and 8 months (n=4–5). No difference was observed between 2–5 months of age. At 8 months-of-age, HIV-1 Tg rats showed significantly fewer TH+ neurons as compared to age-matched controls (p=0.002; n=4–5). B. Representative images of TH immunoreactivity (3,3-diaminobenzidine staining: brown, black) in the SN at 8 months-of-age. C. At 5 months of age, TH immunoreactivity in the SN showed no difference between F344 and HIV-1 rats with DAB staining or fluorescence (green) staining. Iba-1+ microglia showed similar morphology across groups as shown by immunostaining for Iba-1 (red, DAB). GFAP staining (DAB) showed no difference between groups.

    Journal: Neurotoxicity research

    Article Title: Age-related decrease in tyrosine hydroxylase immunoreactivity in the substantia nigra and region-specific changes in microglia morphology in HIV-1 Tg rats

    doi: 10.1007/s12640-019-00077-z

    Figure Lengend Snippet: Tyrosine Hydroxylase Immunoreactivity and Unbiased Stereology. A. Estimates of TH+ neuronal number between 2 and 8 months of age. Scatter graph of individual values (% of F344 control) at each age. Bar graphs of mean +/− SEM for 2–5 months (n=9) and 8 months (n=4–5). No difference was observed between 2–5 months of age. At 8 months-of-age, HIV-1 Tg rats showed significantly fewer TH+ neurons as compared to age-matched controls (p=0.002; n=4–5). B. Representative images of TH immunoreactivity (3,3-diaminobenzidine staining: brown, black) in the SN at 8 months-of-age. C. At 5 months of age, TH immunoreactivity in the SN showed no difference between F344 and HIV-1 rats with DAB staining or fluorescence (green) staining. Iba-1+ microglia showed similar morphology across groups as shown by immunostaining for Iba-1 (red, DAB). GFAP staining (DAB) showed no difference between groups.

    Article Snippet: Rinsed sections were incubated using Vectastain Elite ABC kit for 90 min at RT, washed in 1xTBS and treated with 3-diaminobenzidine (DAB; Agilent Technologies, Santa Clara, CA) [10 mg DAB in 40 ml 1xTBS] and chromogen with nickel chloride amplification.

    Techniques: Staining, Fluorescence, Immunostaining

    Vimentin (Vim) expression in canine mammary sarcomas of various histological type. (A) Representative images of CMSs obtained under the Olympus BX41 microscope. Positive staining for Vim is observed as brown precipitate in the cytoplasm of neoplastic cells. The EnVision + System-HRP detection system was used, and the signal was visualized with chromogen 3,3-diaminobenzidine 3-3' (DAB). (B) The graph represents integrated optical density (IOD) of vimentin-positive cells in canine mammary sarcomas. The colorimetric intensity of IHC-stained antigen spots was determined in a computer-assisted image analyzer (Olympus Microimage™ Image Analysis version 4.0 for Windows, USA), and the color intensity of the antigen spot is expressed as mean pixel optical density on a 1–256 scale. The results are presented as the mean (±SEM) from all tumors in each group. Data was processed in Prism 5.00 software (GraphPad Software, California, USA) using one-way ANOVA and Tukey's HSD post-hoc test. P-values

    Journal: BMC Veterinary Research

    Article Title: Retrospective study and immunohistochemical analysis of canine mammary sarcomas

    doi: 10.1186/1746-6148-9-248

    Figure Lengend Snippet: Vimentin (Vim) expression in canine mammary sarcomas of various histological type. (A) Representative images of CMSs obtained under the Olympus BX41 microscope. Positive staining for Vim is observed as brown precipitate in the cytoplasm of neoplastic cells. The EnVision + System-HRP detection system was used, and the signal was visualized with chromogen 3,3-diaminobenzidine 3-3' (DAB). (B) The graph represents integrated optical density (IOD) of vimentin-positive cells in canine mammary sarcomas. The colorimetric intensity of IHC-stained antigen spots was determined in a computer-assisted image analyzer (Olympus Microimage™ Image Analysis version 4.0 for Windows, USA), and the color intensity of the antigen spot is expressed as mean pixel optical density on a 1–256 scale. The results are presented as the mean (±SEM) from all tumors in each group. Data was processed in Prism 5.00 software (GraphPad Software, California, USA) using one-way ANOVA and Tukey's HSD post-hoc test. P-values

    Article Snippet: The sections were washed, covered with diaminobenzidine chromogen (DAKO) and counterstained with Mayer's hematoxylin for 10 min.

    Techniques: Expressing, Microscopy, Staining, Immunohistochemistry, Software

    Histological and immunohistochemical images of canine mammary fibrosarcoma. The histological sample was stained with the standard hematoxylin and eosin (H-E) method. Positive immunostaining (nuclear or cytoplasmic) was observed as brown precipitate. The EnVision + System-HRP detection system was used, and the signal was visualized with chromogen 3,3-diaminobenzidine 3-3' (DAB). Arrows indicate positive nuclear staining of cells. An asterisk (*) indicates a negatively stained sarcomatous area. Images were obtained under the Olympus BX41 microscope. Original magnification: H-E (x400, arrow indicates a mitotic figure), Vim (x400), α-SMA (x400), Des (x400), Ki67 (x400), PR (x200; arrow indicates PR-positive epithelial cells of the mammary gland duct); PR (x400), p53 (x400).

    Journal: BMC Veterinary Research

    Article Title: Retrospective study and immunohistochemical analysis of canine mammary sarcomas

    doi: 10.1186/1746-6148-9-248

    Figure Lengend Snippet: Histological and immunohistochemical images of canine mammary fibrosarcoma. The histological sample was stained with the standard hematoxylin and eosin (H-E) method. Positive immunostaining (nuclear or cytoplasmic) was observed as brown precipitate. The EnVision + System-HRP detection system was used, and the signal was visualized with chromogen 3,3-diaminobenzidine 3-3' (DAB). Arrows indicate positive nuclear staining of cells. An asterisk (*) indicates a negatively stained sarcomatous area. Images were obtained under the Olympus BX41 microscope. Original magnification: H-E (x400, arrow indicates a mitotic figure), Vim (x400), α-SMA (x400), Des (x400), Ki67 (x400), PR (x200; arrow indicates PR-positive epithelial cells of the mammary gland duct); PR (x400), p53 (x400).

    Article Snippet: The sections were washed, covered with diaminobenzidine chromogen (DAKO) and counterstained with Mayer's hematoxylin for 10 min.

    Techniques: Immunohistochemistry, Staining, Immunostaining, Microscopy

    Histological and immunohistochemical images of canine mammary liposarcoma. The histological sample was stained with the standard hematoxylin and eosin (H-E) method. Immunopositivity (nuclear or cytoplasmic) is shown as brown precipitate in neoplastic cells. The EnVision + System-HRP detection system was used, and the signal was visualized with chromogen 3,3-diaminobenzidine 3-3' (DAB). Arrows indicate positive nuclear staining of cells. The images were obtained under the Olympus BX41 microscope. Original magnification: H-E (x400), Vim (x400), Ki67 (x1000), PR (x400), p53 (x400).

    Journal: BMC Veterinary Research

    Article Title: Retrospective study and immunohistochemical analysis of canine mammary sarcomas

    doi: 10.1186/1746-6148-9-248

    Figure Lengend Snippet: Histological and immunohistochemical images of canine mammary liposarcoma. The histological sample was stained with the standard hematoxylin and eosin (H-E) method. Immunopositivity (nuclear or cytoplasmic) is shown as brown precipitate in neoplastic cells. The EnVision + System-HRP detection system was used, and the signal was visualized with chromogen 3,3-diaminobenzidine 3-3' (DAB). Arrows indicate positive nuclear staining of cells. The images were obtained under the Olympus BX41 microscope. Original magnification: H-E (x400), Vim (x400), Ki67 (x1000), PR (x400), p53 (x400).

    Article Snippet: The sections were washed, covered with diaminobenzidine chromogen (DAKO) and counterstained with Mayer's hematoxylin for 10 min.

    Techniques: Immunohistochemistry, Staining, Microscopy

    Histological and immunohistochemical photographs of other canine mammary sarcomas. The histological sample was stained with the standard hematoxylin and eosin (H-E) method. Immunopositivity (nuclear or cytoplasmic) is shown as brown precipitate in neoplastic cells. The EnVision + System-HRP detection system was used, and the signal was visualized with chromogen 3,3-diaminobenzidine 3-3' (DAB). Arrows indicate positive nuclear staining of cells. The images were obtained under the Olympus BX41 microscope. Original magnification: H-E (x100), Vim (x400), Ki67 (x400), PR (x400), p53 (x1000).

    Journal: BMC Veterinary Research

    Article Title: Retrospective study and immunohistochemical analysis of canine mammary sarcomas

    doi: 10.1186/1746-6148-9-248

    Figure Lengend Snippet: Histological and immunohistochemical photographs of other canine mammary sarcomas. The histological sample was stained with the standard hematoxylin and eosin (H-E) method. Immunopositivity (nuclear or cytoplasmic) is shown as brown precipitate in neoplastic cells. The EnVision + System-HRP detection system was used, and the signal was visualized with chromogen 3,3-diaminobenzidine 3-3' (DAB). Arrows indicate positive nuclear staining of cells. The images were obtained under the Olympus BX41 microscope. Original magnification: H-E (x100), Vim (x400), Ki67 (x400), PR (x400), p53 (x1000).

    Article Snippet: The sections were washed, covered with diaminobenzidine chromogen (DAKO) and counterstained with Mayer's hematoxylin for 10 min.

    Techniques: Immunohistochemistry, Staining, Microscopy

    Histological and immunohistochemical images of canine mammary osteosarcoma. The histological sample was stained with the standard hematoxylin and eosin (H-E) method. Immunopositivity (nuclear or cytoplasmic) is shown as brown precipitate in neoplastic cells. The EnVision + System-HRP detection system was used, and the signal was visualized with chromogen 3,3-diaminobenzidine 3-3' (DAB). Arrows indicate positive nuclear staining of cells. An asterisk (*) indicates a negatively stained sarcomatous area. Images were obtained under the Olympus BX41 microscope. Original magnification: H-E (x400), Vim (x400), α-SMA (x400), Ki67 (x400), ERα (x200, x400), PR (x400), p53 (x400).

    Journal: BMC Veterinary Research

    Article Title: Retrospective study and immunohistochemical analysis of canine mammary sarcomas

    doi: 10.1186/1746-6148-9-248

    Figure Lengend Snippet: Histological and immunohistochemical images of canine mammary osteosarcoma. The histological sample was stained with the standard hematoxylin and eosin (H-E) method. Immunopositivity (nuclear or cytoplasmic) is shown as brown precipitate in neoplastic cells. The EnVision + System-HRP detection system was used, and the signal was visualized with chromogen 3,3-diaminobenzidine 3-3' (DAB). Arrows indicate positive nuclear staining of cells. An asterisk (*) indicates a negatively stained sarcomatous area. Images were obtained under the Olympus BX41 microscope. Original magnification: H-E (x400), Vim (x400), α-SMA (x400), Ki67 (x400), ERα (x200, x400), PR (x400), p53 (x400).

    Article Snippet: The sections were washed, covered with diaminobenzidine chromogen (DAKO) and counterstained with Mayer's hematoxylin for 10 min.

    Techniques: Immunohistochemistry, Staining, Microscopy

    Representative images of immunohistochemical staining, developed with 3,3′-Diaminobenzidine tetrahydrochloride and counter stained with hematoxylin for Col III in rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups, and (D) the statistical analysis. Scale bar represents 100 µm. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Intensity-dependent effect of treadmill running on rat Achilles tendon

    doi: 10.3892/etm.2018.6084

    Figure Lengend Snippet: Representative images of immunohistochemical staining, developed with 3,3′-Diaminobenzidine tetrahydrochloride and counter stained with hematoxylin for Col III in rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups, and (D) the statistical analysis. Scale bar represents 100 µm. *P

    Article Snippet: Sections were subsequently incubated with horseradish peroxidase conjugated goat anti-mouse Imunoglobulin G (1:200; cat. no. sc2005; Santa Cruz Biotechnology, CA, USA) for 1 h at room temperature, developed with 3,3′-Diaminobenzidine tetrahydrochloride (DAKO; Agilent Technologies, Inc., Santa Clara, CA, USA) and counter-stained in hematoxylin.

    Techniques: Immunohistochemistry, Staining

    Representative images of the immunohistochemical staining, developed with 3,3′-Diaminobenzidine tetrahydrochloride and counter stained with hematoxylin for Col I in the rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups and (D) the statistical analysis. Scale bar represents 100 µm. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Intensity-dependent effect of treadmill running on rat Achilles tendon

    doi: 10.3892/etm.2018.6084

    Figure Lengend Snippet: Representative images of the immunohistochemical staining, developed with 3,3′-Diaminobenzidine tetrahydrochloride and counter stained with hematoxylin for Col I in the rat Achilles tendon. (A) CON, (B) MIR and (C) HIR groups and (D) the statistical analysis. Scale bar represents 100 µm. *P

    Article Snippet: Sections were subsequently incubated with horseradish peroxidase conjugated goat anti-mouse Imunoglobulin G (1:200; cat. no. sc2005; Santa Cruz Biotechnology, CA, USA) for 1 h at room temperature, developed with 3,3′-Diaminobenzidine tetrahydrochloride (DAKO; Agilent Technologies, Inc., Santa Clara, CA, USA) and counter-stained in hematoxylin.

    Techniques: Immunohistochemistry, Staining

    Meprin-α is expressed in cancer cells. Immunostaining for meprin-α on tissue sections from formalin-fixed and paraffin-embedded colorectal tissue samples using a rabbit polyclonal primary antibody together with a peroxidase-coupled secondary antibody and diaminobenzidine as chromogenic substrate. Counter stain: hematoxylin. Objective magnification: 40×. Bar = 50 µm. (A) In normal colon mucosa meprin-α is barely detectable. Occasionally, signals were detected at the base of crypt regions in the cytosol of cells with apically localized nuclei (asterisks). The specificity of these signals and the identity of the cells was not further investigated. (B) Representative sample of a meprin-α positive primary tumor stage III. Tumors exhibit a mosaic expression pattern. In cancer cell clusters, cells with strong signals for meprin-α (arrows) are intermixed with cells that show weak signals or no signal at all. Cells in the stroma do not express meprin-α (C) Representative sample of meprin-α positive primary tumor stage IV showing clusters of meprin-α positive cancer cells (arrows). (D) Cancer cells express meprin-α (arrows) in liver metastases (Met). The surrounding normal liver tissue (Hep) is only weakly stained.

    Journal: PLoS ONE

    Article Title: Enhanced Activity of Meprin-?, a Pro-Migratory and Pro-Angiogenic Protease, in Colorectal Cancer

    doi: 10.1371/journal.pone.0026450

    Figure Lengend Snippet: Meprin-α is expressed in cancer cells. Immunostaining for meprin-α on tissue sections from formalin-fixed and paraffin-embedded colorectal tissue samples using a rabbit polyclonal primary antibody together with a peroxidase-coupled secondary antibody and diaminobenzidine as chromogenic substrate. Counter stain: hematoxylin. Objective magnification: 40×. Bar = 50 µm. (A) In normal colon mucosa meprin-α is barely detectable. Occasionally, signals were detected at the base of crypt regions in the cytosol of cells with apically localized nuclei (asterisks). The specificity of these signals and the identity of the cells was not further investigated. (B) Representative sample of a meprin-α positive primary tumor stage III. Tumors exhibit a mosaic expression pattern. In cancer cell clusters, cells with strong signals for meprin-α (arrows) are intermixed with cells that show weak signals or no signal at all. Cells in the stroma do not express meprin-α (C) Representative sample of meprin-α positive primary tumor stage IV showing clusters of meprin-α positive cancer cells (arrows). (D) Cancer cells express meprin-α (arrows) in liver metastases (Met). The surrounding normal liver tissue (Hep) is only weakly stained.

    Article Snippet: A.) in 25 mM Tris-HCl, pH 7.5, 140 mM NaCl, and diaminobenzidine (Immuno Pure Metal Enhanced DAB; Pierce Chemical Co., Rockford, IL, U. S.

    Techniques: Immunostaining, Staining, Expressing

    Identifying 3,3′-diaminobenzidine (DAB)-stained regions using the NanoSuit method. a – g Colon tissue immunostained using an antibody against smooth muscle actin with color development via DAB staining (brown). b Staining of the section shown in a with 1% gold (III) chloride. The DAB-staining intensity of the low-vacuum scanning electron microscopy (Lv-SEM) image was selectively enhanced as white color, taken in backscattered electron (BSE) mode. c Image of a control section without gold (III) chloride treatment. d DAB staining of the muscularis propria of a colon tissue section. e Lv-SEM image, taken in BSE mode. f DAB-stained section of a magnified area of the muscularis propria and blood vessels. g Three-dimensional (3D) structure of the correlative region shown in f . Lv-SEM image, taken in mixed BSE and SE mode. h DAB-stained dendritic cells (DCs) of the human epidermis. i Field emission-scanning electron microscopy (FE-SEM) image of DAB-stained DCs after treatment with 1% gold chloride, taken in yttrium–aluminum–garnet BSE mode. j 3D structure of DAB-stained DCs in an FE-SEM image, taken in secondary electron mode. The scale bars represent 2 mm ( a – c ), 500 ( d , e ), 50 ( f , g ), and 10 μm ( h – j )

    Journal: Laboratory Investigation; a Journal of Technical Methods and Pathology

    Article Title: The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy

    doi: 10.1038/s41374-019-0309-7

    Figure Lengend Snippet: Identifying 3,3′-diaminobenzidine (DAB)-stained regions using the NanoSuit method. a – g Colon tissue immunostained using an antibody against smooth muscle actin with color development via DAB staining (brown). b Staining of the section shown in a with 1% gold (III) chloride. The DAB-staining intensity of the low-vacuum scanning electron microscopy (Lv-SEM) image was selectively enhanced as white color, taken in backscattered electron (BSE) mode. c Image of a control section without gold (III) chloride treatment. d DAB staining of the muscularis propria of a colon tissue section. e Lv-SEM image, taken in BSE mode. f DAB-stained section of a magnified area of the muscularis propria and blood vessels. g Three-dimensional (3D) structure of the correlative region shown in f . Lv-SEM image, taken in mixed BSE and SE mode. h DAB-stained dendritic cells (DCs) of the human epidermis. i Field emission-scanning electron microscopy (FE-SEM) image of DAB-stained DCs after treatment with 1% gold chloride, taken in yttrium–aluminum–garnet BSE mode. j 3D structure of DAB-stained DCs in an FE-SEM image, taken in secondary electron mode. The scale bars represent 2 mm ( a – c ), 500 ( d , e ), 50 ( f , g ), and 10 μm ( h – j )

    Article Snippet: After washing in phosphate-buffered solution (PBS; 137 mM NaCl, 10 mM phosphate, 2.7 mM KCl, pH 7.4), the sections were incubated with a peroxidase- or biotin-conjugated universal immunoenzyme polymer anti-mouse or anti-rabbit solution (Nichirei Biosciences) and visualized using streptavidin-conjugated gold (40 nm; Abcam, Cambridge, UK) or 3,3′-diaminobenzidine (DAB) (DAKO).

    Techniques: Staining, Electron Microscopy

    Direct observation of gold particles in immunostained sections using the NanoSuit method. a – c Staining of a breast cancer section with a human epidermal growth factor receptor 2 (HER2) score of 3+. a HER2 expression detected by 3,3′-diaminobenzidine (DAB) staining (brown). HER2 expression observed by detection of 40-nm gold particle signals as multiple white dots in a low-vacuum scanning electron microscopy (Lv-SEM) image taken in backscattered electron (BSE) mode ( b ), or in an FE-SEM taken in secondary electron mode ( c ). Staining of a breast cancer section with a HER2 score of 0. No HER2 expression was detected by DAB staining (brown) ( d ), in an Lv-SEM image taken in BSE mode ( e ), or in an FE-SEM image taken in secondary electron mode ( f ). g , h Detection of human cytomegalovirus (CMV) with an anti-gB antibody conjugated with 40-nm gold particles. The white square in g was magnified and multiple 40-nm gold particles on the surface of human CMV particles are shown in h . i F-actin detected with 40-nm gold particles on TNT-like structures. The scale bars represent 200 ( a , d ), 20 ( b , e ), 2 ( c , f , g , i ), and 1 μm ( h )

    Journal: Laboratory Investigation; a Journal of Technical Methods and Pathology

    Article Title: The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy

    doi: 10.1038/s41374-019-0309-7

    Figure Lengend Snippet: Direct observation of gold particles in immunostained sections using the NanoSuit method. a – c Staining of a breast cancer section with a human epidermal growth factor receptor 2 (HER2) score of 3+. a HER2 expression detected by 3,3′-diaminobenzidine (DAB) staining (brown). HER2 expression observed by detection of 40-nm gold particle signals as multiple white dots in a low-vacuum scanning electron microscopy (Lv-SEM) image taken in backscattered electron (BSE) mode ( b ), or in an FE-SEM taken in secondary electron mode ( c ). Staining of a breast cancer section with a HER2 score of 0. No HER2 expression was detected by DAB staining (brown) ( d ), in an Lv-SEM image taken in BSE mode ( e ), or in an FE-SEM image taken in secondary electron mode ( f ). g , h Detection of human cytomegalovirus (CMV) with an anti-gB antibody conjugated with 40-nm gold particles. The white square in g was magnified and multiple 40-nm gold particles on the surface of human CMV particles are shown in h . i F-actin detected with 40-nm gold particles on TNT-like structures. The scale bars represent 200 ( a , d ), 20 ( b , e ), 2 ( c , f , g , i ), and 1 μm ( h )

    Article Snippet: After washing in phosphate-buffered solution (PBS; 137 mM NaCl, 10 mM phosphate, 2.7 mM KCl, pH 7.4), the sections were incubated with a peroxidase- or biotin-conjugated universal immunoenzyme polymer anti-mouse or anti-rabbit solution (Nichirei Biosciences) and visualized using streptavidin-conjugated gold (40 nm; Abcam, Cambridge, UK) or 3,3′-diaminobenzidine (DAB) (DAKO).

    Techniques: Staining, Expressing, Electron Microscopy