Article Title: Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection
Figure Lengend Snippet: Histopathological images of lung sections at 6 h post-infection. (A) In situ presence of enolase, a control protein. (B) The presence of serine proteinase during infection in lung tissue. White arrows evidence fungal cells and black arrows evidence the labeling occurring also outside fungal cells. Scale bars are indicated. The polyclonal antibodies anti-enolase 25 at 1:150 dilution and anti-serine-proteinase 24 at 1:150 dilution, previously obtained, were used in A and B, respectively. The chromogen 3.3′ diaminobenzidine tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin and examined by light microscopy.
Article Snippet: After, the slides were incubated with Mouse-on Mouse HRP-Polymer (Biocare Medical, #MM620G) for 30 min at 37°C for signal detection, followed by addition of Diaminobenzidine (DAB, Dako, #K3468-1).
Techniques: Infection, In Situ, Labeling, Light Microscopy