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Fig. 5 Western blot of cecropin-intein carrier. (a) Immunoblotting with anti-CBD antibody; (b) Immunoblotting with anti-E. coli ER2566 cell lysate antibody. Lane 1: Molecular weight marker (Bio-Rad) (kDa); Lane 2: Chitin beds with cecropin-intein carrier before cleavage with DTT. Membrane was revealed with <t>diaminobenzidine.</t> SDS-PAGE used for immunoblotting in sections a and b are shown as reference to the right of revealed membranes. The cecropin-intein carrier detection with anti-CBD antibody in shown by an arrow in section a.
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Fig. 5 Western blot of cecropin-intein carrier. (a) Immunoblotting with anti-CBD antibody; (b) Immunoblotting with anti-E. coli ER2566 cell lysate antibody. Lane 1: Molecular weight marker (Bio-Rad) (kDa); Lane 2: Chitin beds with cecropin-intein carrier before cleavage with DTT. Membrane was revealed with <t>diaminobenzidine.</t> SDS-PAGE used for immunoblotting in sections a and b are shown as reference to the right of revealed membranes. The cecropin-intein carrier detection with anti-CBD antibody in shown by an arrow in section a.
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Fig. 5 Western blot of cecropin-intein carrier. (a) Immunoblotting with anti-CBD antibody; (b) Immunoblotting with anti-E. coli ER2566 cell lysate antibody. Lane 1: Molecular weight marker (Bio-Rad) (kDa); Lane 2: Chitin beds with cecropin-intein carrier before cleavage with DTT. Membrane was revealed with <t>diaminobenzidine.</t> SDS-PAGE used for immunoblotting in sections a and b are shown as reference to the right of revealed membranes. The cecropin-intein carrier detection with anti-CBD antibody in shown by an arrow in section a.
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Fig. 5 Western blot of cecropin-intein carrier. (a) Immunoblotting with anti-CBD antibody; (b) Immunoblotting with anti-E. coli ER2566 cell lysate antibody. Lane 1: Molecular weight marker (Bio-Rad) (kDa); Lane 2: Chitin beds with cecropin-intein carrier before cleavage with DTT. Membrane was revealed with <t>diaminobenzidine.</t> SDS-PAGE used for immunoblotting in sections a and b are shown as reference to the right of revealed membranes. The cecropin-intein carrier detection with anti-CBD antibody in shown by an arrow in section a.
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Fig. 5 Western blot of cecropin-intein carrier. (a) Immunoblotting with anti-CBD antibody; (b) Immunoblotting with anti-E. coli ER2566 cell lysate antibody. Lane 1: Molecular weight marker (Bio-Rad) (kDa); Lane 2: Chitin beds with cecropin-intein carrier before cleavage with DTT. Membrane was revealed with <t>diaminobenzidine.</t> SDS-PAGE used for immunoblotting in sections a and b are shown as reference to the right of revealed membranes. The cecropin-intein carrier detection with anti-CBD antibody in shown by an arrow in section a.
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Fig. 5 Western blot of cecropin-intein carrier. (a) Immunoblotting with anti-CBD antibody; (b) Immunoblotting with anti-E. coli ER2566 cell lysate antibody. Lane 1: Molecular weight marker (Bio-Rad) (kDa); Lane 2: Chitin beds with cecropin-intein carrier before cleavage with DTT. Membrane was revealed with <t>diaminobenzidine.</t> SDS-PAGE used for immunoblotting in sections a and b are shown as reference to the right of revealed membranes. The cecropin-intein carrier detection with anti-CBD antibody in shown by an arrow in section a.
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Fig. 5 Western blot of cecropin-intein carrier. (a) Immunoblotting with anti-CBD antibody; (b) Immunoblotting with anti-E. coli ER2566 cell lysate antibody. Lane 1: Molecular weight marker (Bio-Rad) (kDa); Lane 2: Chitin beds with cecropin-intein carrier before cleavage with DTT. Membrane was revealed with <t>diaminobenzidine.</t> SDS-PAGE used for immunoblotting in sections a and b are shown as reference to the right of revealed membranes. The cecropin-intein carrier detection with anti-CBD antibody in shown by an arrow in section a.
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MedChemExpress 3 3 diaminobenzidine dab substrate
Fig. 5 Western blot of cecropin-intein carrier. (a) Immunoblotting with anti-CBD antibody; (b) Immunoblotting with anti-E. coli ER2566 cell lysate antibody. Lane 1: Molecular weight marker (Bio-Rad) (kDa); Lane 2: Chitin beds with cecropin-intein carrier before cleavage with DTT. Membrane was revealed with <t>diaminobenzidine.</t> SDS-PAGE used for immunoblotting in sections a and b are shown as reference to the right of revealed membranes. The cecropin-intein carrier detection with anti-CBD antibody in shown by an arrow in section a.
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Image Search Results


Fig. 5 Western blot of cecropin-intein carrier. (a) Immunoblotting with anti-CBD antibody; (b) Immunoblotting with anti-E. coli ER2566 cell lysate antibody. Lane 1: Molecular weight marker (Bio-Rad) (kDa); Lane 2: Chitin beds with cecropin-intein carrier before cleavage with DTT. Membrane was revealed with diaminobenzidine. SDS-PAGE used for immunoblotting in sections a and b are shown as reference to the right of revealed membranes. The cecropin-intein carrier detection with anti-CBD antibody in shown by an arrow in section a.

Journal: Electronic Journal of Biotechnology

Article Title: Intein-mediated expression of cecropin in Escherichia coli

doi: 10.2225/vol15-issue2-fulltext-2

Figure Lengend Snippet: Fig. 5 Western blot of cecropin-intein carrier. (a) Immunoblotting with anti-CBD antibody; (b) Immunoblotting with anti-E. coli ER2566 cell lysate antibody. Lane 1: Molecular weight marker (Bio-Rad) (kDa); Lane 2: Chitin beds with cecropin-intein carrier before cleavage with DTT. Membrane was revealed with diaminobenzidine. SDS-PAGE used for immunoblotting in sections a and b are shown as reference to the right of revealed membranes. The cecropin-intein carrier detection with anti-CBD antibody in shown by an arrow in section a.

Article Snippet: For the visual detection 3,3’ diaminobenzidine (DAB) (Pierce) was used.

Techniques: Western Blot, Molecular Weight, Marker, Membrane, SDS Page