diaminobenzidine Search Results


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  • 99
    Millipore diaminobencidine
    EP 2 receptor expression increases in distal human pulmonary arteries from PAH patients. Representative immunohistochemical staining in serial sections of a pulmonary artery from a control ( A ) and a PAH patient ( B ) showing the prostanoid EP 2 receptor (EP 2 ), the smooth muscle marker, the α-smooth muscle actin (α-SMA) and the endothelial cell marker, CD-31. Staining was visualised by <t>diaminobenzidine</t> (brown) in sections counterstained with haematoxylin (blue) and quantified using ImageJ. Grey images represent the digitisation of staining in arterial sections, where adventitial staining has been excluded to focus on the expression in muscle and the endothelium. Bars are 100 µm and is the same for each section. The data are expressed as the pixel area of the respective staining for CD-31, α-SMA, and EP 2 receptor ( C ) or as the ratio of EP 2 /α-SMA+CD31 area staining ( D ). Excluded from the analysis of data presented in parts ( C ) and ( D ) was staining in plexiform lesions. * p
    Diaminobencidine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1507 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore 3 3 diaminobenzadine tetrahydrochloride
    EP 2 receptor expression increases in distal human pulmonary arteries from PAH patients. Representative immunohistochemical staining in serial sections of a pulmonary artery from a control ( A ) and a PAH patient ( B ) showing the prostanoid EP 2 receptor (EP 2 ), the smooth muscle marker, the α-smooth muscle actin (α-SMA) and the endothelial cell marker, CD-31. Staining was visualised by <t>diaminobenzidine</t> (brown) in sections counterstained with haematoxylin (blue) and quantified using ImageJ. Grey images represent the digitisation of staining in arterial sections, where adventitial staining has been excluded to focus on the expression in muscle and the endothelium. Bars are 100 µm and is the same for each section. The data are expressed as the pixel area of the respective staining for CD-31, α-SMA, and EP 2 receptor ( C ) or as the ratio of EP 2 /α-SMA+CD31 area staining ( D ). Excluded from the analysis of data presented in parts ( C ) and ( D ) was staining in plexiform lesions. * p
    3 3 Diaminobenzadine Tetrahydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Agilent technologies diaminobenzidin
    EP 2 receptor expression increases in distal human pulmonary arteries from PAH patients. Representative immunohistochemical staining in serial sections of a pulmonary artery from a control ( A ) and a PAH patient ( B ) showing the prostanoid EP 2 receptor (EP 2 ), the smooth muscle marker, the α-smooth muscle actin (α-SMA) and the endothelial cell marker, CD-31. Staining was visualised by <t>diaminobenzidine</t> (brown) in sections counterstained with haematoxylin (blue) and quantified using ImageJ. Grey images represent the digitisation of staining in arterial sections, where adventitial staining has been excluded to focus on the expression in muscle and the endothelium. Bars are 100 µm and is the same for each section. The data are expressed as the pixel area of the respective staining for CD-31, α-SMA, and EP 2 receptor ( C ) or as the ratio of EP 2 /α-SMA+CD31 area staining ( D ). Excluded from the analysis of data presented in parts ( C ) and ( D ) was staining in plexiform lesions. * p
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    Agilent technologies diaminobenzidine
    Histopathological analyzes of mice lung injected with immuno stimulants. After Lung tissues in (A) Carrageenan (B) Heat-killed yeast cells and (C) Zymozan injected mice. The presence of serine proteinase in the tissue was not detected. Scale bars are indicated. The polyclonal antibodies anti-serine-proteinase at 1:150 dilution was used. The chromogen 3.3′ <t>diaminobenzidine</t> tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin, and examined by light microscopy.
    Diaminobenzidine, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 11560 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Axxora diaminobenzidine
    Histopathological analyzes of mice lung injected with immuno stimulants. After Lung tissues in (A) Carrageenan (B) Heat-killed yeast cells and (C) Zymozan injected mice. The presence of serine proteinase in the tissue was not detected. Scale bars are indicated. The polyclonal antibodies anti-serine-proteinase at 1:150 dilution was used. The chromogen 3.3′ <t>diaminobenzidine</t> tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin, and examined by light microscopy.
    Diaminobenzidine, supplied by Axxora, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson diaminobenzidine
    Histopathological analyzes of mice lung injected with immuno stimulants. After Lung tissues in (A) Carrageenan (B) Heat-killed yeast cells and (C) Zymozan injected mice. The presence of serine proteinase in the tissue was not detected. Scale bars are indicated. The polyclonal antibodies anti-serine-proteinase at 1:150 dilution was used. The chromogen 3.3′ <t>diaminobenzidine</t> tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin, and examined by light microscopy.
    Diaminobenzidine, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim diaminobenzidine
    Histopathological analyzes of mice lung injected with immuno stimulants. After Lung tissues in (A) Carrageenan (B) Heat-killed yeast cells and (C) Zymozan injected mice. The presence of serine proteinase in the tissue was not detected. Scale bars are indicated. The polyclonal antibodies anti-serine-proteinase at 1:150 dilution was used. The chromogen 3.3′ <t>diaminobenzidine</t> tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin, and examined by light microscopy.
    Diaminobenzidine, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boster Bio diaminobenzidine
    Photomicrographs showing increased VEGF immunohistochemical staining in the VEGF + NSCs group. Immunohistochemistry for VEGF using <t>diaminobenzidine</t> in cerebral cortex neurons of cerebral palsy neonatal rats 7 days after transplantation of control (A, sham operation), cerebral palsy (B), phosphate-buffered saline (C), VEGF 165 -transfected neural stem cells (D), or NSCs alone (E). VEGF-positive cells are shown with arrows. Scale bars: 100 μm. VEGF: Vascular endothelial growth factor; NSCs: neural stem cells.
    Diaminobenzidine, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 828 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Dojindo Labs diaminobenzidine
    Photomicrographs showing increased VEGF immunohistochemical staining in the VEGF + NSCs group. Immunohistochemistry for VEGF using <t>diaminobenzidine</t> in cerebral cortex neurons of cerebral palsy neonatal rats 7 days after transplantation of control (A, sham operation), cerebral palsy (B), phosphate-buffered saline (C), VEGF 165 -transfected neural stem cells (D), or NSCs alone (E). VEGF-positive cells are shown with arrows. Scale bars: 100 μm. VEGF: Vascular endothelial growth factor; NSCs: neural stem cells.
    Diaminobenzidine, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 92/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FUJIFILM diaminobenzidine
    Photomicrographs showing increased VEGF immunohistochemical staining in the VEGF + NSCs group. Immunohistochemistry for VEGF using <t>diaminobenzidine</t> in cerebral cortex neurons of cerebral palsy neonatal rats 7 days after transplantation of control (A, sham operation), cerebral palsy (B), phosphate-buffered saline (C), VEGF 165 -transfected neural stem cells (D), or NSCs alone (E). VEGF-positive cells are shown with arrows. Scale bars: 100 μm. VEGF: Vascular endothelial growth factor; NSCs: neural stem cells.
    Diaminobenzidine, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Genmed diaminobenzidine
    Photomicrographs showing increased VEGF immunohistochemical staining in the VEGF + NSCs group. Immunohistochemistry for VEGF using <t>diaminobenzidine</t> in cerebral cortex neurons of cerebral palsy neonatal rats 7 days after transplantation of control (A, sham operation), cerebral palsy (B), phosphate-buffered saline (C), VEGF 165 -transfected neural stem cells (D), or NSCs alone (E). VEGF-positive cells are shown with arrows. Scale bars: 100 μm. VEGF: Vascular endothelial growth factor; NSCs: neural stem cells.
    Diaminobenzidine, supplied by Genmed, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Golden Bridge International diaminobenzidine
    Influence of quercetin nanoemulsion on hepatic gene expression related to cholesterol efflux. mRNA levels of LXRα, ABCG5 and ABCG8 ( A ) and CYP7A1 ( B ) were measured by real-time qPCR and normalized to β-actin. The results were expressed as the fold change compared to HC. CYP7A1 enzyme activity was measured by a rat CYP7A1 ELISA kit and normalized to their relative microsomal protein contents ( C ). Immunohistochemistry staining for CYP7A1was performed using a <t>diaminobenzidine-based</t> staining technique ( D ). Values are expressed as means ± SEM ( n = 8). * p
    Diaminobenzidine, supplied by Golden Bridge International, used in various techniques. Bioz Stars score: 91/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Nichirei diaminobenzidine
    OPN expression in the cerebral cortex of NMO patients. (A) High OPN expression level in the white matter of NMO patients. Scale bar: 2.5 mm. OPN expression around the boundary area between white and gray matter in the patient with NMO (B), MS (C), and AD (D). Scale bars: 200 μm. The dashed lines indicate the boundary between white and gray matter. w, white matter; g, gray matter. OPN expression in astrocytes (E; arrows), neurons (E; white arrowheads), and oligodendroglias (F; arrowheads) of the cerebral sections of NMO patients. Scale bars: 50 μm. (G and H) Serial cerebral section from NMO patients demonstrating perivascular infiltration of numerous macrophages expressing OPN. The area in the rectangle in (G) is shown at higher magnification in (H). The arrowheads in (H) indicate macrophages. Scale bars: 200 μm (G), and 50 μm (H). OPN staining was performed using mAb against OPN (clone 53) on paraffin-embedded sections (n = 3) from two patients per disease. Photos in (B–H) are immunoperoxidase-stained with <t>3,3′-diaminobenzidine</t> and hematoxylin counterstain. (I and J) Representative immunofluorescence staining of cerebral sections of an NMO patient. (I) Staining with OPN (green) and GFAP (red: astrocytes). (J) Staining with OPN (green) and CNPase (red: oligodendroglias). Nuclei were stained blue with Hoechst 33342. Scale bars: 50 μm.
    Diaminobenzidine, supplied by Nichirei, used in various techniques. Bioz Stars score: 94/100, based on 521 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche diaminobenzidine
    OPN expression in the cerebral cortex of NMO patients. (A) High OPN expression level in the white matter of NMO patients. Scale bar: 2.5 mm. OPN expression around the boundary area between white and gray matter in the patient with NMO (B), MS (C), and AD (D). Scale bars: 200 μm. The dashed lines indicate the boundary between white and gray matter. w, white matter; g, gray matter. OPN expression in astrocytes (E; arrows), neurons (E; white arrowheads), and oligodendroglias (F; arrowheads) of the cerebral sections of NMO patients. Scale bars: 50 μm. (G and H) Serial cerebral section from NMO patients demonstrating perivascular infiltration of numerous macrophages expressing OPN. The area in the rectangle in (G) is shown at higher magnification in (H). The arrowheads in (H) indicate macrophages. Scale bars: 200 μm (G), and 50 μm (H). OPN staining was performed using mAb against OPN (clone 53) on paraffin-embedded sections (n = 3) from two patients per disease. Photos in (B–H) are immunoperoxidase-stained with <t>3,3′-diaminobenzidine</t> and hematoxylin counterstain. (I and J) Representative immunofluorescence staining of cerebral sections of an NMO patient. (I) Staining with OPN (green) and GFAP (red: astrocytes). (J) Staining with OPN (green) and CNPase (red: oligodendroglias). Nuclei were stained blue with Hoechst 33342. Scale bars: 50 μm.
    Diaminobenzidine, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher diaminobenzidine
    Detection of TGF-β production in vivo in spleens of L. donovani -infected hamsters. Spleen sections (4 μm thick) from noninfected hamsters (A) and from hamsters infected with L. donovani for 60 days (B) were fixed with acetone and incubated with anti-TGF-β MAb 1D11.16, washed four times with PBS plus 0.05% Tween 20, and incubated with a rabbit anti-mouse immunoglobulin-peroxidase conjugate for 1 h at room temperature. The slides were washed and incubated with 1 mg of <t>diaminobenzidine</t> per ml and 0.01% hydrogen peroxide in Tris-HCl (0.1 M [pH 7.2]). Brown granules indicate the presence of TGF-β in the spleens of infected animals. Magnification, ×500.
    Diaminobenzidine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Zhongshan Golden Bridge Company diaminobenzidine
    Detection of TGF-β production in vivo in spleens of L. donovani -infected hamsters. Spleen sections (4 μm thick) from noninfected hamsters (A) and from hamsters infected with L. donovani for 60 days (B) were fixed with acetone and incubated with anti-TGF-β MAb 1D11.16, washed four times with PBS plus 0.05% Tween 20, and incubated with a rabbit anti-mouse immunoglobulin-peroxidase conjugate for 1 h at room temperature. The slides were washed and incubated with 1 mg of <t>diaminobenzidine</t> per ml and 0.01% hydrogen peroxide in Tris-HCl (0.1 M [pH 7.2]). Brown granules indicate the presence of TGF-β in the spleens of infected animals. Magnification, ×500.
    Diaminobenzidine, supplied by Zhongshan Golden Bridge Company, used in various techniques. Bioz Stars score: 92/100, based on 535 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Zytomed diaminobenzidine
    Detection of TGF-β production in vivo in spleens of L. donovani -infected hamsters. Spleen sections (4 μm thick) from noninfected hamsters (A) and from hamsters infected with L. donovani for 60 days (B) were fixed with acetone and incubated with anti-TGF-β MAb 1D11.16, washed four times with PBS plus 0.05% Tween 20, and incubated with a rabbit anti-mouse immunoglobulin-peroxidase conjugate for 1 h at room temperature. The slides were washed and incubated with 1 mg of <t>diaminobenzidine</t> per ml and 0.01% hydrogen peroxide in Tris-HCl (0.1 M [pH 7.2]). Brown granules indicate the presence of TGF-β in the spleens of infected animals. Magnification, ×500.
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    Abcam diaminobenzidine
    Detection of TGF-β production in vivo in spleens of L. donovani -infected hamsters. Spleen sections (4 μm thick) from noninfected hamsters (A) and from hamsters infected with L. donovani for 60 days (B) were fixed with acetone and incubated with anti-TGF-β MAb 1D11.16, washed four times with PBS plus 0.05% Tween 20, and incubated with a rabbit anti-mouse immunoglobulin-peroxidase conjugate for 1 h at room temperature. The slides were washed and incubated with 1 mg of <t>diaminobenzidine</t> per ml and 0.01% hydrogen peroxide in Tris-HCl (0.1 M [pH 7.2]). Brown granules indicate the presence of TGF-β in the spleens of infected animals. Magnification, ×500.
    Diaminobenzidine, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beijing Solarbio Science diaminobenzidine
    Detection of TGF-β production in vivo in spleens of L. donovani -infected hamsters. Spleen sections (4 μm thick) from noninfected hamsters (A) and from hamsters infected with L. donovani for 60 days (B) were fixed with acetone and incubated with anti-TGF-β MAb 1D11.16, washed four times with PBS plus 0.05% Tween 20, and incubated with a rabbit anti-mouse immunoglobulin-peroxidase conjugate for 1 h at room temperature. The slides were washed and incubated with 1 mg of <t>diaminobenzidine</t> per ml and 0.01% hydrogen peroxide in Tris-HCl (0.1 M [pH 7.2]). Brown granules indicate the presence of TGF-β in the spleens of infected animals. Magnification, ×500.
    Diaminobenzidine, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beyotime diaminobenzidine
    Expression analysis of β-catenin in pancreatic ductal adenocarcinoma and normal tissues. β-catenin was mainly distributed along the cytoplasm border in normal tissues. In PPPDE1-low cancer, β-catenin was particularly prone to appear in the nucleus. However, β-catenin was rarely found to invade the nucleus in PPPDE1-high cancer. PPPDE1, PPPDE peptidase domain-containing protein 1; PPPDE1-high cancer, cancer tissue with a PPPDE1 staining score of > 3; PPPDE1-low cancer, cancer tissue with a PPPDE1 staining score of ≤3 (stain, <t>diaminobenzidine;</t> magnification, ×200).
    Diaminobenzidine, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad diaminobenzidine
    Production of HSP70 (left) and iNOS (right) in traumatized liver. Immunohistochemical staining of tissue harvested 24 h after blunt liver trauma. Enzymatic reaction was performed with <t>diaminobenzidine;</t> methyl green counterstain.
    Diaminobenzidine, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    EP 2 receptor expression increases in distal human pulmonary arteries from PAH patients. Representative immunohistochemical staining in serial sections of a pulmonary artery from a control ( A ) and a PAH patient ( B ) showing the prostanoid EP 2 receptor (EP 2 ), the smooth muscle marker, the α-smooth muscle actin (α-SMA) and the endothelial cell marker, CD-31. Staining was visualised by diaminobenzidine (brown) in sections counterstained with haematoxylin (blue) and quantified using ImageJ. Grey images represent the digitisation of staining in arterial sections, where adventitial staining has been excluded to focus on the expression in muscle and the endothelium. Bars are 100 µm and is the same for each section. The data are expressed as the pixel area of the respective staining for CD-31, α-SMA, and EP 2 receptor ( C ) or as the ratio of EP 2 /α-SMA+CD31 area staining ( D ). Excluded from the analysis of data presented in parts ( C ) and ( D ) was staining in plexiform lesions. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Prostanoid EP2 Receptors Are Up-Regulated in Human Pulmonary Arterial Hypertension: A Key Anti-Proliferative Target for Treprostinil in Smooth Muscle Cells

    doi: 10.3390/ijms19082372

    Figure Lengend Snippet: EP 2 receptor expression increases in distal human pulmonary arteries from PAH patients. Representative immunohistochemical staining in serial sections of a pulmonary artery from a control ( A ) and a PAH patient ( B ) showing the prostanoid EP 2 receptor (EP 2 ), the smooth muscle marker, the α-smooth muscle actin (α-SMA) and the endothelial cell marker, CD-31. Staining was visualised by diaminobenzidine (brown) in sections counterstained with haematoxylin (blue) and quantified using ImageJ. Grey images represent the digitisation of staining in arterial sections, where adventitial staining has been excluded to focus on the expression in muscle and the endothelium. Bars are 100 µm and is the same for each section. The data are expressed as the pixel area of the respective staining for CD-31, α-SMA, and EP 2 receptor ( C ) or as the ratio of EP 2 /α-SMA+CD31 area staining ( D ). Excluded from the analysis of data presented in parts ( C ) and ( D ) was staining in plexiform lesions. * p

    Article Snippet: Sections were then developed utilizing avidin-conjugated horseradish peroxidase (HRP) and staining was visualised with diaminobenzidine (Sigma-Aldrich) in sections lightly counterstained with haematoxylin (Sigma-Aldrich).

    Techniques: Expressing, Immunohistochemistry, Staining, Marker

    In situ detection of H 2 O 2 using 3,3′-diaminobenzidine (DAB) staining on rubber tree leaves after treatment with 5 mM SA. The rubber tree leaves were sprayed with either DW as a control or 5 mM SA. Leaf pieces were collected and stained after treatment at different time points (0, 3, 6, 12, 24, 48, 72, 96 and 120 h). Pictures represent three independent biological replicates. The dark brown precipitates indicate the presence and distribution of H 2 O 2 in plant cells, which could be visualized using a light microscope (scale bars = 100 μm).

    Journal: International Journal of Molecular Sciences

    Article Title: Salicylic Acid Induces Resistance in Rubber Tree against Phytophthora palmivora

    doi: 10.3390/ijms19071883

    Figure Lengend Snippet: In situ detection of H 2 O 2 using 3,3′-diaminobenzidine (DAB) staining on rubber tree leaves after treatment with 5 mM SA. The rubber tree leaves were sprayed with either DW as a control or 5 mM SA. Leaf pieces were collected and stained after treatment at different time points (0, 3, 6, 12, 24, 48, 72, 96 and 120 h). Pictures represent three independent biological replicates. The dark brown precipitates indicate the presence and distribution of H 2 O 2 in plant cells, which could be visualized using a light microscope (scale bars = 100 μm).

    Article Snippet: In situ detection of H2 O2 content in rubber tree leaf cells was performed by staining with 3, 3′-diaminobenzidine (DAB; Sigma Chem.

    Techniques: In Situ, Staining, Light Microscopy

    Histopathological analyzes of mice lung injected with immuno stimulants. After Lung tissues in (A) Carrageenan (B) Heat-killed yeast cells and (C) Zymozan injected mice. The presence of serine proteinase in the tissue was not detected. Scale bars are indicated. The polyclonal antibodies anti-serine-proteinase at 1:150 dilution was used. The chromogen 3.3′ diaminobenzidine tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin, and examined by light microscopy.

    Journal: Virulence

    Article Title: Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection

    doi: 10.1080/21505594.2017.1355660

    Figure Lengend Snippet: Histopathological analyzes of mice lung injected with immuno stimulants. After Lung tissues in (A) Carrageenan (B) Heat-killed yeast cells and (C) Zymozan injected mice. The presence of serine proteinase in the tissue was not detected. Scale bars are indicated. The polyclonal antibodies anti-serine-proteinase at 1:150 dilution was used. The chromogen 3.3′ diaminobenzidine tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin, and examined by light microscopy.

    Article Snippet: After, the slides were incubated with Mouse-on Mouse HRP-Polymer (Biocare Medical, #MM620G) for 30 min at 37°C for signal detection, followed by addition of Diaminobenzidine (DAB, Dako, #K3468-1).

    Techniques: Mouse Assay, Injection, Light Microscopy

    Histopathological images of lung sections at 6 h post-infection. (A) In situ presence of enolase, a control protein. (B) The presence of serine proteinase during infection in lung tissue. White arrows evidence fungal cells and black arrows evidence the labeling occurring also outside fungal cells. Scale bars are indicated. The polyclonal antibodies anti-enolase 25 at 1:150 dilution and anti-serine-proteinase 24 at 1:150 dilution, previously obtained, were used in A and B, respectively. The chromogen 3.3′ diaminobenzidine tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin and examined by light microscopy.

    Journal: Virulence

    Article Title: Paracoccidioides brasiliensis presents metabolic reprogramming and secretes a serine proteinase during murine infection

    doi: 10.1080/21505594.2017.1355660

    Figure Lengend Snippet: Histopathological images of lung sections at 6 h post-infection. (A) In situ presence of enolase, a control protein. (B) The presence of serine proteinase during infection in lung tissue. White arrows evidence fungal cells and black arrows evidence the labeling occurring also outside fungal cells. Scale bars are indicated. The polyclonal antibodies anti-enolase 25 at 1:150 dilution and anti-serine-proteinase 24 at 1:150 dilution, previously obtained, were used in A and B, respectively. The chromogen 3.3′ diaminobenzidine tetrahydrocloride (DAB, Dako, #K3468-1) was used, and sections were then counterstained with Mayer's hematoxylin and examined by light microscopy.

    Article Snippet: After, the slides were incubated with Mouse-on Mouse HRP-Polymer (Biocare Medical, #MM620G) for 30 min at 37°C for signal detection, followed by addition of Diaminobenzidine (DAB, Dako, #K3468-1).

    Techniques: Infection, In Situ, Labeling, Light Microscopy

    Photomicrographs showing increased VEGF immunohistochemical staining in the VEGF + NSCs group. Immunohistochemistry for VEGF using diaminobenzidine in cerebral cortex neurons of cerebral palsy neonatal rats 7 days after transplantation of control (A, sham operation), cerebral palsy (B), phosphate-buffered saline (C), VEGF 165 -transfected neural stem cells (D), or NSCs alone (E). VEGF-positive cells are shown with arrows. Scale bars: 100 μm. VEGF: Vascular endothelial growth factor; NSCs: neural stem cells.

    Journal: Neural Regeneration Research

    Article Title: Response of the sensorimotor cortex of cerebral palsy rats receiving transplantation of vascular endothelial growth factor 165-transfected neural stem cells

    doi: 10.4103/1673-5374.141785

    Figure Lengend Snippet: Photomicrographs showing increased VEGF immunohistochemical staining in the VEGF + NSCs group. Immunohistochemistry for VEGF using diaminobenzidine in cerebral cortex neurons of cerebral palsy neonatal rats 7 days after transplantation of control (A, sham operation), cerebral palsy (B), phosphate-buffered saline (C), VEGF 165 -transfected neural stem cells (D), or NSCs alone (E). VEGF-positive cells are shown with arrows. Scale bars: 100 μm. VEGF: Vascular endothelial growth factor; NSCs: neural stem cells.

    Article Snippet: Immunoreactivity was detected with diaminobenzidine (Wuhan Boster Biological), then sections were counter-stained with hematoxylin-eosin and mounted with gum.

    Techniques: Immunohistochemistry, Staining, Transplantation Assay, Transfection

    Influence of quercetin nanoemulsion on hepatic gene expression related to cholesterol efflux. mRNA levels of LXRα, ABCG5 and ABCG8 ( A ) and CYP7A1 ( B ) were measured by real-time qPCR and normalized to β-actin. The results were expressed as the fold change compared to HC. CYP7A1 enzyme activity was measured by a rat CYP7A1 ELISA kit and normalized to their relative microsomal protein contents ( C ). Immunohistochemistry staining for CYP7A1was performed using a diaminobenzidine-based staining technique ( D ). Values are expressed as means ± SEM ( n = 8). * p

    Journal: Nutrients

    Article Title: Formulation and Characterization of Quercetin-loaded Oil in Water Nanoemulsion and Evaluation of Hypocholesterolemic Activity in Rats

    doi: 10.3390/nu11020244

    Figure Lengend Snippet: Influence of quercetin nanoemulsion on hepatic gene expression related to cholesterol efflux. mRNA levels of LXRα, ABCG5 and ABCG8 ( A ) and CYP7A1 ( B ) were measured by real-time qPCR and normalized to β-actin. The results were expressed as the fold change compared to HC. CYP7A1 enzyme activity was measured by a rat CYP7A1 ELISA kit and normalized to their relative microsomal protein contents ( C ). Immunohistochemistry staining for CYP7A1was performed using a diaminobenzidine-based staining technique ( D ). Values are expressed as means ± SEM ( n = 8). * p

    Article Snippet: The sections were stained with diaminobenzidine (Golden Bridge International, Inc., Bothell, WA, USA) and counterstained with Mayer hematoxylin (ScyTek, Logan, UT, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Activity Assay, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining

    OPN expression in the cerebral cortex of NMO patients. (A) High OPN expression level in the white matter of NMO patients. Scale bar: 2.5 mm. OPN expression around the boundary area between white and gray matter in the patient with NMO (B), MS (C), and AD (D). Scale bars: 200 μm. The dashed lines indicate the boundary between white and gray matter. w, white matter; g, gray matter. OPN expression in astrocytes (E; arrows), neurons (E; white arrowheads), and oligodendroglias (F; arrowheads) of the cerebral sections of NMO patients. Scale bars: 50 μm. (G and H) Serial cerebral section from NMO patients demonstrating perivascular infiltration of numerous macrophages expressing OPN. The area in the rectangle in (G) is shown at higher magnification in (H). The arrowheads in (H) indicate macrophages. Scale bars: 200 μm (G), and 50 μm (H). OPN staining was performed using mAb against OPN (clone 53) on paraffin-embedded sections (n = 3) from two patients per disease. Photos in (B–H) are immunoperoxidase-stained with 3,3′-diaminobenzidine and hematoxylin counterstain. (I and J) Representative immunofluorescence staining of cerebral sections of an NMO patient. (I) Staining with OPN (green) and GFAP (red: astrocytes). (J) Staining with OPN (green) and CNPase (red: oligodendroglias). Nuclei were stained blue with Hoechst 33342. Scale bars: 50 μm.

    Journal: BBA Clinical

    Article Title: Increased cerebrospinal fluid osteopontin levels and its involvement in macrophage infiltration in neuromyelitis optica

    doi: 10.1016/j.bbacli.2015.01.003

    Figure Lengend Snippet: OPN expression in the cerebral cortex of NMO patients. (A) High OPN expression level in the white matter of NMO patients. Scale bar: 2.5 mm. OPN expression around the boundary area between white and gray matter in the patient with NMO (B), MS (C), and AD (D). Scale bars: 200 μm. The dashed lines indicate the boundary between white and gray matter. w, white matter; g, gray matter. OPN expression in astrocytes (E; arrows), neurons (E; white arrowheads), and oligodendroglias (F; arrowheads) of the cerebral sections of NMO patients. Scale bars: 50 μm. (G and H) Serial cerebral section from NMO patients demonstrating perivascular infiltration of numerous macrophages expressing OPN. The area in the rectangle in (G) is shown at higher magnification in (H). The arrowheads in (H) indicate macrophages. Scale bars: 200 μm (G), and 50 μm (H). OPN staining was performed using mAb against OPN (clone 53) on paraffin-embedded sections (n = 3) from two patients per disease. Photos in (B–H) are immunoperoxidase-stained with 3,3′-diaminobenzidine and hematoxylin counterstain. (I and J) Representative immunofluorescence staining of cerebral sections of an NMO patient. (I) Staining with OPN (green) and GFAP (red: astrocytes). (J) Staining with OPN (green) and CNPase (red: oligodendroglias). Nuclei were stained blue with Hoechst 33342. Scale bars: 50 μm.

    Article Snippet: After washing with PBS three times, peroxidase was visualized with 3,3′-diaminobenzidine, using the Histofine DAB substrate kit (Nichirei Corp., Cat# 425011).

    Techniques: Expressing, Mass Spectrometry, Staining, Immunofluorescence

    Detection of TGF-β production in vivo in spleens of L. donovani -infected hamsters. Spleen sections (4 μm thick) from noninfected hamsters (A) and from hamsters infected with L. donovani for 60 days (B) were fixed with acetone and incubated with anti-TGF-β MAb 1D11.16, washed four times with PBS plus 0.05% Tween 20, and incubated with a rabbit anti-mouse immunoglobulin-peroxidase conjugate for 1 h at room temperature. The slides were washed and incubated with 1 mg of diaminobenzidine per ml and 0.01% hydrogen peroxide in Tris-HCl (0.1 M [pH 7.2]). Brown granules indicate the presence of TGF-β in the spleens of infected animals. Magnification, ×500.

    Journal: Infection and Immunity

    Article Title: Transforming Growth Factor ? and Immunosuppression in Experimental Visceral Leishmaniasis

    doi:

    Figure Lengend Snippet: Detection of TGF-β production in vivo in spleens of L. donovani -infected hamsters. Spleen sections (4 μm thick) from noninfected hamsters (A) and from hamsters infected with L. donovani for 60 days (B) were fixed with acetone and incubated with anti-TGF-β MAb 1D11.16, washed four times with PBS plus 0.05% Tween 20, and incubated with a rabbit anti-mouse immunoglobulin-peroxidase conjugate for 1 h at room temperature. The slides were washed and incubated with 1 mg of diaminobenzidine per ml and 0.01% hydrogen peroxide in Tris-HCl (0.1 M [pH 7.2]). Brown granules indicate the presence of TGF-β in the spleens of infected animals. Magnification, ×500.

    Article Snippet: The slides were washed four times and incubated with 1 mg of diaminobenzidine (Pierce Chemical Company) per ml and 0.01% hydrogen peroxide in 100 mM Tris-HCl (pH 7.2).

    Techniques: In Vivo, Infection, Incubation

    Expression analysis of β-catenin in pancreatic ductal adenocarcinoma and normal tissues. β-catenin was mainly distributed along the cytoplasm border in normal tissues. In PPPDE1-low cancer, β-catenin was particularly prone to appear in the nucleus. However, β-catenin was rarely found to invade the nucleus in PPPDE1-high cancer. PPPDE1, PPPDE peptidase domain-containing protein 1; PPPDE1-high cancer, cancer tissue with a PPPDE1 staining score of > 3; PPPDE1-low cancer, cancer tissue with a PPPDE1 staining score of ≤3 (stain, diaminobenzidine; magnification, ×200).

    Journal: Oncology Letters

    Article Title: Implications of PPPDE1 expression in the distribution of plakoglobin and β-catenin in pancreatic ductal adenocarcinoma

    doi: 10.3892/ol.2014.2279

    Figure Lengend Snippet: Expression analysis of β-catenin in pancreatic ductal adenocarcinoma and normal tissues. β-catenin was mainly distributed along the cytoplasm border in normal tissues. In PPPDE1-low cancer, β-catenin was particularly prone to appear in the nucleus. However, β-catenin was rarely found to invade the nucleus in PPPDE1-high cancer. PPPDE1, PPPDE peptidase domain-containing protein 1; PPPDE1-high cancer, cancer tissue with a PPPDE1 staining score of > 3; PPPDE1-low cancer, cancer tissue with a PPPDE1 staining score of ≤3 (stain, diaminobenzidine; magnification, ×200).

    Article Snippet: The sections were stained using an avidin-biotin-peroxidase complex (ABC) method and visualized with diaminobenzidine (DAB; Beyotime, Haimen, China), according to the manufacturer’s instructions.

    Techniques: Expressing, Staining

    Expression analysis of PPPDE1 in pancreatic ductal adenocarcinoma and normal tissues. The expression of PPPDE1 was markedly decreased in cancer tissues compared with that in normal tissues, showing the lowest expression in poorly differentiated pancreatic cancer. PPPDE1, PPPDE peptidase domain-containing protein 1; PPPDE1-high cancer, cancer tissue with a PPPDE1 staining score of > 3; PPPDE1-low cancer, cancer tissue with a PPPDE1 staining score of ≤3 (stain, diaminobenzidine; magnification, ×200).

    Journal: Oncology Letters

    Article Title: Implications of PPPDE1 expression in the distribution of plakoglobin and β-catenin in pancreatic ductal adenocarcinoma

    doi: 10.3892/ol.2014.2279

    Figure Lengend Snippet: Expression analysis of PPPDE1 in pancreatic ductal adenocarcinoma and normal tissues. The expression of PPPDE1 was markedly decreased in cancer tissues compared with that in normal tissues, showing the lowest expression in poorly differentiated pancreatic cancer. PPPDE1, PPPDE peptidase domain-containing protein 1; PPPDE1-high cancer, cancer tissue with a PPPDE1 staining score of > 3; PPPDE1-low cancer, cancer tissue with a PPPDE1 staining score of ≤3 (stain, diaminobenzidine; magnification, ×200).

    Article Snippet: The sections were stained using an avidin-biotin-peroxidase complex (ABC) method and visualized with diaminobenzidine (DAB; Beyotime, Haimen, China), according to the manufacturer’s instructions.

    Techniques: Expressing, Staining

    Expression analysis of plakoglobin in pancreatic ductal adenocarcinoma and normal tissues. Plakoglobin expression was highest in normal tissues compared with that in cancer tissues, and was mainly distributed in the membrane and cytoplasm border of cells. A greater proportion of cells exhibited low membrane plakoglobin expression in PPPDE1-low cancer compared with that in PPPDE1-high cancer. However, cytoplasmic staining of plakoglobin remained evident in PPPDE1-low cancer, although its total abundance was markedly decreased compared with that of normal cells. PPPDE1, PPPDE peptidase domain-containing protein 1; PPPDE1-high cancer, cancer tissue with a PPPDE1 staining score of > 3; PPPDE1-low cancer, cancer tissue with a PPPDE1 staining score of ≤3 (stain, diaminobenzidine; magnification, ×200).

    Journal: Oncology Letters

    Article Title: Implications of PPPDE1 expression in the distribution of plakoglobin and β-catenin in pancreatic ductal adenocarcinoma

    doi: 10.3892/ol.2014.2279

    Figure Lengend Snippet: Expression analysis of plakoglobin in pancreatic ductal adenocarcinoma and normal tissues. Plakoglobin expression was highest in normal tissues compared with that in cancer tissues, and was mainly distributed in the membrane and cytoplasm border of cells. A greater proportion of cells exhibited low membrane plakoglobin expression in PPPDE1-low cancer compared with that in PPPDE1-high cancer. However, cytoplasmic staining of plakoglobin remained evident in PPPDE1-low cancer, although its total abundance was markedly decreased compared with that of normal cells. PPPDE1, PPPDE peptidase domain-containing protein 1; PPPDE1-high cancer, cancer tissue with a PPPDE1 staining score of > 3; PPPDE1-low cancer, cancer tissue with a PPPDE1 staining score of ≤3 (stain, diaminobenzidine; magnification, ×200).

    Article Snippet: The sections were stained using an avidin-biotin-peroxidase complex (ABC) method and visualized with diaminobenzidine (DAB; Beyotime, Haimen, China), according to the manufacturer’s instructions.

    Techniques: Expressing, Staining

    Production of HSP70 (left) and iNOS (right) in traumatized liver. Immunohistochemical staining of tissue harvested 24 h after blunt liver trauma. Enzymatic reaction was performed with diaminobenzidine; methyl green counterstain.

    Journal: Comparative Medicine

    Article Title: Development and Characterization of a Rat Model of Nonpenetrating Liver Trauma

    doi:

    Figure Lengend Snippet: Production of HSP70 (left) and iNOS (right) in traumatized liver. Immunohistochemical staining of tissue harvested 24 h after blunt liver trauma. Enzymatic reaction was performed with diaminobenzidine; methyl green counterstain.

    Article Snippet: Biotinylated secondary IgG antibody (1:250 in 10% serum) was added for 30 min at room temperature, followed by peroxidase-conjugated streptavidin (1:600) at room temperature for 30 min. Enzymatic reactions were performed by using diaminobenzidine (BioRad).

    Techniques: Immunohistochemistry, Staining