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Image Search Results
Journal: Immunity, Inflammation and Disease
Article Title: Mapping an epitope in EBNA‐1 that is recognized by monoclonal antibodies to EBNA‐1 that cross‐react with dsDNA
doi: 10.1002/iid3.119
Figure Lengend Snippet: 16D2 cross‐reacts with dsDNA. 16D2 was tested by ELISA for binding to EBNA‐1 (A) and dsDNA (B). Binding to dsDNA was confirmed by immunostaining of the kinetoplast of Crithidia luciliae (C). Immunostaining of an isotype control MAb is shown in D (D). 16D2 was tested by ELISA for reactivity with a panel of antigens; Sm, LPS, BSA, and proteinase 3 (PR3) (E). Results are representative of three experiments. ELISA values were obtained in triplicate. Error bars indicate standard deviations of triplicate values.
Article Snippet: Ready to use
Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Immunostaining, Control
Journal: PLoS Genetics
Article Title: Initial Genomics of the Human Nucleolus
doi: 10.1371/journal.pgen.1000889
Figure Lengend Snippet: (A) Histograms show the frequency of the nucleolar localisation of NADs and control chromosomal regions detected by 3D FISH in HeLa cervix carcinoma and IMR90 diploid fibroblast cells. Percentage of nucleolus-associated alleles is shown on the left. Red diamond indicates target, green ones negative controls, whereas yellow diamond indicates the chromosome X pericentromeric and blue diamond the 5S cluster positive controls, respectively (see for further BAC details). Single light optical sections of HeLa nuclei are shown on the right. BAC hybridization signals of RP11-90G23 target, RP5-915N17 positive control and RP11-81M8 negative control BACs are shown in green, nucleolar staining in red and DAPI counterstain in blue (scale bars: 5 µm). (B) α-H3K27Me3, α-centromere, α-active Pol II and α-B23/nucleophosmin immunostaining of HeLa and IMR90 cells. α-H3K27Me3, α-centromere and α-active Pol II signals are shown in green, nucleolar staining in red and DAPI counterstain in blue (scale bars: 5 µm).
Article Snippet: In localisation experiments α-B23/nucleophosmin (Sigma, B0556), α-H3K27Me3 (Upstate, 07-449), α-active Pol II (Covance, MMS-129R),
Techniques: Control, Hybridization, Positive Control, Negative Control, Staining, Immunostaining