Journal: Endocrinology

Article Title: Identification and cloning of a novel androgen-responsive gene, uridine diphosphoglucose dehydrogenase, in human breast cancer cells.

doi: 10.1210/endo.140.10.7071

Figure Lengend Snippet: FIG. 1. Differential display PCR analysis. A, Human ZR-75–1 breast cancer cells were grown for 48 h in the presence of estradiol (10210 M) alone (control) or in combination with DHT (1029 M). Total RNA was isolated from control and DHT-treated cells and used for cDNA syn- thesis in the presence of oligo(dT) primer and Moloney murine leu- kemia virus reverse transcriptase. A fraction of each cDNA was am- plified by PCR using P4 and T1 primers, [a-33P]dATP, and dNTP mix. The products were analyzed on a denaturing 6% acrylamide gel. The gel was dried and exposed to x-ray film. B, The differentially displayed cDNA was amplified by PCR using the P4 and T1 primers and was separated on a 1% agarose gel along with DNA size markers.

Article Snippet: 17b-Estradiol (E2) and DHT were purchased from Steraloids (Wilton, NH).

Techniques: Control, Isolation, Virus, Reverse Transcription, Acrylamide Gel Assay, Amplification, Agarose Gel Electrophoresis

Journal: Endocrinology

Article Title: Identification and cloning of a novel androgen-responsive gene, uridine diphosphoglucose dehydrogenase, in human breast cancer cells.

doi: 10.1210/endo.140.10.7071

Figure Lengend Snippet: FIG. 2. Northern blot analysis of androgen-regulated mRNAs. Total RNA was isolated from ZR-75–1 cells grown for 48 h in the presence of estradiol (10210 M) alone (control) or in combination with DHT (1029

Article Snippet: 17b-Estradiol (E2) and DHT were purchased from Steraloids (Wilton, NH).

Techniques: Northern Blot, Isolation, Control

Journal: Endocrinology

Article Title: Identification and cloning of a novel androgen-responsive gene, uridine diphosphoglucose dehydrogenase, in human breast cancer cells.

doi: 10.1210/endo.140.10.7071

Figure Lengend Snippet: FIG. 6. Time course of DHT treatment on UDPGDH mRNA levels in ZR-75–1 cells. ZR-75–1 cells were grown for 0, 1, 6, 24, and 48 h in medium containing estradiol (10210 M) supplemented with DHT (1029

Article Snippet: 17b-Estradiol (E2) and DHT were purchased from Steraloids (Wilton, NH).

Techniques:

Journal: Endocrinology

Article Title: Identification and cloning of a novel androgen-responsive gene, uridine diphosphoglucose dehydrogenase, in human breast cancer cells.

doi: 10.1210/endo.140.10.7071

Figure Lengend Snippet: FIG. 7. Northern analysis of UDPGDH mRNA levels after DHT, cy- cloheximide, actinomycin D, and hydroxyflutamide treatment in ZR- 75–1 cells. ZR-75–1 cells were grown in medium containing estradiol (10210 M) supplemented with 1029 M DHT, DHT plus cycloheximide, DHT plus actinomycin D, and DHT plus hydroxyflutamide (3 3 1026

Article Snippet: 17b-Estradiol (E2) and DHT were purchased from Steraloids (Wilton, NH).

Techniques: Northern Blot

Journal: Foods (Basel, Switzerland)

Article Title: Analysis of the Beneficial Effects of Probiotics on the Gut-Prostate Axis Using Prostatic Co-Culture Model.

doi: 10.3390/foods13223647

Figure Lengend Snippet: Figure 2. Permeability and adhesion study on CaCo-2 cells. In (A), Cell viability is measured by using the MTT test; in (B), TEER Value using EVOM3; in (C), SCFA analysis is conducted using an ELISA kit; in (D), butyric acid analysis is carried out using an ELISA kit; in (E) the analysis of aggregation activity and in (F) the analysis of hydrophobicity activity. Data are mean ± SD of five independent experiments performed in triplicates compared to control values (0% line). In (A,C–F), * p < 0.05 vs. control; ** p < 0.05 vs. single agents. In (B), p < 0.05 vs. control; ** p < 0.05 vs. single agents.

Article Snippet: According to the manufacturer, a Human Dihydrotestosterone ELISA Kit (Cusabio, Houston, TX, USA) was used to measure DHT in co-culture prostatic supernatants [43].

Techniques: Permeability, Enzyme-linked Immunosorbent Assay, Activity Assay, Control

Journal: Foods (Basel, Switzerland)

Article Title: Analysis of the Beneficial Effects of Probiotics on the Gut-Prostate Axis Using Prostatic Co-Culture Model.

doi: 10.3390/foods13223647

Figure Lengend Snippet: Figure 3. Integrity analysis. In (A), the analysis of claudin-1 measured by ELISA test; in (B), the analysis of ZO-1 by ELISA test; in (C), the analysis of occludin-1 by ELISA kit. Data are mean ± SD of five independent experiments performed in triplicates vs. control values (0% line) and * p < 0.05 vs. control; ** p < 0.05 vs. single agents.

Article Snippet: According to the manufacturer, a Human Dihydrotestosterone ELISA Kit (Cusabio, Houston, TX, USA) was used to measure DHT in co-culture prostatic supernatants [43].

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Foods (Basel, Switzerland)

Article Title: Analysis of the Beneficial Effects of Probiotics on the Gut-Prostate Axis Using Prostatic Co-Culture Model.

doi: 10.3390/foods13223647

Figure Lengend Snippet: Figure 4. Analysis of oxidative stress and inflammation. All of the following results are derived from the ELISA kit: (A) ROS generation; (B) SOD levels; (C) TNF-α production; (D) IL-6 production; and (E) IL-10 production. The mean ± SD of five separate studies conducted in triplicate is shown, along with * p < 0.05 vs. control, ** p < 0.05 vs. single drugs, and # p < 0.05 vs. DHT, in comparison to control values (0% line).

Article Snippet: According to the manufacturer, a Human Dihydrotestosterone ELISA Kit (Cusabio, Houston, TX, USA) was used to measure DHT in co-culture prostatic supernatants [43].

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Control, Comparison

Journal: Foods (Basel, Switzerland)

Article Title: Analysis of the Beneficial Effects of Probiotics on the Gut-Prostate Axis Using Prostatic Co-Culture Model.

doi: 10.3390/foods13223647

Figure Lengend Snippet: Figure 5. Mechanism analyses for prostate hyperplasia. Testosterone levels (A), 5α-reductase levels (B), DHT levels (C), AR levels (D), and PSA levels (E) are all derived from the ELISA kit. Values are mean ± SD from five independent tests in triplicates compared to control values (0% line). * p < 0.05 vs. control; ** p < 0.05 vs. single agents; # p < 0.05 vs. DHT.

Article Snippet: According to the manufacturer, a Human Dihydrotestosterone ELISA Kit (Cusabio, Houston, TX, USA) was used to measure DHT in co-culture prostatic supernatants [43].

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: Foods (Basel, Switzerland)

Article Title: Analysis of the Beneficial Effects of Probiotics on the Gut-Prostate Axis Using Prostatic Co-Culture Model.

doi: 10.3390/foods13223647

Figure Lengend Snippet: Figure 6. Serotonin and proliferation studies. The following results are derived from an ELISA kit: (A) 5-Htr1a levels, (B) serotonin production, (C) Ki67 expression, and (D) proliferation rate. The mean ± SD of five separate, duplicate tests is presented, along with * p < 0.05 vs. control, ** p < 0.05 vs. single drugs, and # p < 0.05 vs. DHT, in comparison to control values (0% line).

Article Snippet: According to the manufacturer, a Human Dihydrotestosterone ELISA Kit (Cusabio, Houston, TX, USA) was used to measure DHT in co-culture prostatic supernatants [43].

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Expressing, Control, Comparison

Journal: International journal of molecular medicine

Article Title: Development of finasteride polymer microspheres for systemic application in androgenic alopecia.

doi: 10.3892/ijmm.2019.4149

Figure Lengend Snippet: Figure 2. FNS‑loaded microspheres effectively prevent 5α‑reductase activity in a testosterone‑induced androgenic alopecia mouse model. (A) Schematic diagram of the study protocol. The mice were administered with the test compounds orally or subcutaneously for 8 weeks. (B) Images of the dorsal skin at 8 weeks. Individual data were obtained using enzyme‑linked immunosorbent assays for (C) testosterone and (D) DHT in serum. The DHT levels decreased 4 weeks after orally‑applied FNS and FNS‑loaded microspheres administration. All data are presented as the mean ± standard error of the mean from three independent experiments. *P<0.05 and **P<0.01, vs. microspheres‑treated group. FNS, finasteride; TP, testosterone propionate; DHT, dihydrotestosterone.

Article Snippet: Serum testosterone (cat. no. CSB‐E05101m) and DHT (cat. no. CSB‐E07880m) were measured via enzyme linked immunosorbent assays (ELISA; Cusabio, Wuhan, China).

Techniques: Activity Assay

Journal: Analytical and bioanalytical chemistry

Article Title: Simultaneous measurement of total Estradiol and Testosterone in human serum by isotope dilution liquid chromatography tandem mass spectrometry

doi: 10.1007/s00216-017-0529-x

Figure Lengend Snippet: Steroid hormones used for interference analysis. Their Molecular weight and source of materials are provided.

Article Snippet: 5a-Dihydrotestosterone (DHT) , 290.44 , Cerilliant (Round Rock, TX).

Techniques: Molecular Weight