Journal: American Journal of Cancer Research

Article Title: Gentian violet induces apoptosis and ferroptosis via modulating p53 and MDM2 in hepatocellular carcinoma

doi:

Figure Lengend Snippet: The high expression of MDM2 and p53 was dependent on Hep27. Western blot detection of MDM2, p53 and Hep27 expression in SK-HEP-1 and SMMC-7721 cells at the indicated treatment conditions for 48 h. From left to right for each cell line: DMSO, GV (100 nM for SK-HEP-1 and 300 nM for SMMC-7721), GV in cells transfected with Hep27 siRNA, GV co-treated with Fer-1 (2 μM).

Article Snippet: Knockdown of MDM2, p53 and Hep27 was performed using siRNA technology. siRNA oligos targeting MDM2 (sc-29394), p53 (sc-29435), Hep27 (sc-92153) or non-specific siRNAs were purchased from Santa Cruz Biotechnology (Dallas, TX). siRNAs were transfected into cells by electroporation using Nucleofector Device and the Nucleofector Kit L (Lonza, Basel, Switzerland).

Techniques: Expressing, Western Blot, Transfection

Journal: American Journal of Cancer Research

Article Title: Gentian violet induces apoptosis and ferroptosis via modulating p53 and MDM2 in hepatocellular carcinoma

doi:

Figure Lengend Snippet: The proposed molecular mechanisms of GV-induced killing of HCC. When NOX is inhibited by GV, Hep27 is increased, which leads to the blockade of the ubiquitination and degradation of p53 by MDM2. Consequently, the levels of p53 and MDM2 are increased. GV also directly increases p53 level. Increased p53 and MDM2 levels trigger ferroptosis and mitochondrial apoptosis (through the activation of caspase 8) by a coordinated ROS cross point and downstream signaling cascades. On the other hand, GV also triggers death receptor apoptosis (through the activation of caspase 9) by upregulating death receptors DR4/5 and ligands FAS-ligand and TRAIL.

Article Snippet: Knockdown of MDM2, p53 and Hep27 was performed using siRNA technology. siRNA oligos targeting MDM2 (sc-29394), p53 (sc-29435), Hep27 (sc-92153) or non-specific siRNAs were purchased from Santa Cruz Biotechnology (Dallas, TX). siRNAs were transfected into cells by electroporation using Nucleofector Device and the Nucleofector Kit L (Lonza, Basel, Switzerland).

Techniques: Ubiquitin Proteomics, Activation Assay

Journal: Scientific Reports

Article Title: Apoptosis Signal-regulating Kinase 1 promotes Ochratoxin A-induced renal cytotoxicity

doi: 10.1038/srep08078

Figure Lengend Snippet: Proteins differentially expressed in response to OTA treatment versus control in Scrambled shRNA cell group (118:117). Shading area indicates proteins that also appeared in ASK1 knockdown cell group. Proteins are ordered alphabetically by their gene names

Article Snippet: The DHRS2 antibody (1:200) was purchased from Santa Cruz Biotechnology.

Techniques: Control, shRNA, Knockdown

Journal: Scientific Reports

Article Title: Apoptosis Signal-regulating Kinase 1 promotes Ochratoxin A-induced renal cytotoxicity

doi: 10.1038/srep08078

Figure Lengend Snippet: Proteins differentially expressed in response to OTA treatment versus control in ASK1 knockdown cell group (121:119). Shading area indicates proteins that also appeared in scrambled cell group. Proteins are ordered alphabetically by their gene names

Article Snippet: The DHRS2 antibody (1:200) was purchased from Santa Cruz Biotechnology.

Techniques: Control, Knockdown

Journal: Scientific Reports

Article Title: Apoptosis Signal-regulating Kinase 1 promotes Ochratoxin A-induced renal cytotoxicity

doi: 10.1038/srep08078

Figure Lengend Snippet: Four proteins were selected to validate the alteration trend using Western Blot. Gels were run under the same experimental conditions while images of western blots displayed in cropped format. KRT18 was up-regulated in ASK1 knockdown cell group and PKM was up-regulated in scrambled cell group compared with control (no drug treatment). CDK1 and DHRS2 were both down-regulated in scrambled cell group as well as ASK1 knockdown cell group compared to control. Actin was used as a loading control.

Article Snippet: The DHRS2 antibody (1:200) was purchased from Santa Cruz Biotechnology.

Techniques: Western Blot, Knockdown, Control

Journal: Epigenetics

Article Title: Decreased DHRS2 expression is associated with HDACi resistance and poor prognosis in ovarian cancer

doi: 10.1080/15592294.2019.1656155

Figure Lengend Snippet: Correlation analysis of HDACi sensitivity and DHRS2 expression in adherent cells from the ovary . Pearson correlation coefficients for comparisons of HDAC inhibitor sensitivity data, expressed as the area under concentration-response curves (AUCs), with DHRS2 expression measurements, expressed as log2 robust-multi-array-average values.

Article Snippet: For the RNA interference of DHRS2, a specific siRNA pool targeting DHRS2 was custom-designed and synthesized by Ribobio.

Techniques: Expressing, Concentration Assay