dh5αmcr Search Results


dh5αmcr  (Thermo Fisher)
90
Thermo Fisher dh5αmcr
Bacterial strains and plasmids used in this study
Dh5αmcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dh5αmcr cells  (New England Biolabs)
97
New England Biolabs dh5αmcr cells
Bacterial strains and plasmids used in this study
Dh5αmcr Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e. coli dh5μmcr competent cells  (Bethesda Research Laboratories Inc)
90
Bethesda Research Laboratories Inc e. coli dh5μmcr competent cells
Bacterial strains and plasmids used in this study
E. Coli Dh5μmcr Competent Cells, supplied by Bethesda Research Laboratories Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e. coli dh5αmcr  (Thermo Fisher)
90
Thermo Fisher e. coli dh5αmcr
In vivo integration assay in <t>E.</t> <t>coli.</t> Two different mating experiments were done to confirm that the His6-tagged CTnDOT Int protein expressed in E. coli had all of the functions necessary to perform site-specific recombination in vivo. In the first mating, the plasmid pattDOT (Cmr, pir dependent, containing CTnDOT attachment site attDOT) was mobilized from the donor E. coli pir+ strain BW19851 into the recipient E. coli pir-deficient strain EM24NR carrying the plasmid pINT101 (Apr). The pINT101 plasmid contains the wild-type int gene (under the control of Ptac promoter) from CTnDOT and one of its chromosomal target sites (attB). The Cmr Rfr Apr transconjugants (rifampin resistance from the recipient strain EM24NR) were derived from the cointegrate plasmid due to site-specific recombination between the attDOT and attB sites. The reaction was mediated by the wild-type Int protein expressed from pINT101 in the recipient. The second mating had the same donor and a recipient that carried pINT201. pINT201 has the same attB site and expresses the His6-tagged Int protein. The Cmr Rfr Apr transconjugants were obtained at a similar frequency (10−4/recipient; see Results).
E. Coli Dh5αmcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli strain dh5alphamcr pec k18mob2ptsiexp  (DSMZ)
97
DSMZ escherichia coli strain dh5alphamcr pec k18mob2ptsiexp
In vivo integration assay in <t>E.</t> <t>coli.</t> Two different mating experiments were done to confirm that the His6-tagged CTnDOT Int protein expressed in E. coli had all of the functions necessary to perform site-specific recombination in vivo. In the first mating, the plasmid pattDOT (Cmr, pir dependent, containing CTnDOT attachment site attDOT) was mobilized from the donor E. coli pir+ strain BW19851 into the recipient E. coli pir-deficient strain EM24NR carrying the plasmid pINT101 (Apr). The pINT101 plasmid contains the wild-type int gene (under the control of Ptac promoter) from CTnDOT and one of its chromosomal target sites (attB). The Cmr Rfr Apr transconjugants (rifampin resistance from the recipient strain EM24NR) were derived from the cointegrate plasmid due to site-specific recombination between the attDOT and attB sites. The reaction was mediated by the wild-type Int protein expressed from pINT101 in the recipient. The second mating had the same donor and a recipient that carried pINT201. pINT201 has the same attB site and expresses the His6-tagged Int protein. The Cmr Rfr Apr transconjugants were obtained at a similar frequency (10−4/recipient; see Results).
Escherichia Coli Strain Dh5alphamcr Pec K18mob2ptsiexp, supplied by DSMZ, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e. coli dh5αmcr/pec-k18mob2ptshexp  (DSMZ)
90
DSMZ e. coli dh5αmcr/pec-k18mob2ptshexp
In vivo integration assay in <t>E.</t> <t>coli.</t> Two different mating experiments were done to confirm that the His6-tagged CTnDOT Int protein expressed in E. coli had all of the functions necessary to perform site-specific recombination in vivo. In the first mating, the plasmid pattDOT (Cmr, pir dependent, containing CTnDOT attachment site attDOT) was mobilized from the donor E. coli pir+ strain BW19851 into the recipient E. coli pir-deficient strain EM24NR carrying the plasmid pINT101 (Apr). The pINT101 plasmid contains the wild-type int gene (under the control of Ptac promoter) from CTnDOT and one of its chromosomal target sites (attB). The Cmr Rfr Apr transconjugants (rifampin resistance from the recipient strain EM24NR) were derived from the cointegrate plasmid due to site-specific recombination between the attDOT and attB sites. The reaction was mediated by the wild-type Int protein expressed from pINT101 in the recipient. The second mating had the same donor and a recipient that carried pINT201. pINT201 has the same attB site and expresses the His6-tagged Int protein. The Cmr Rfr Apr transconjugants were obtained at a similar frequency (10−4/recipient; see Results).
E. Coli Dh5αmcr/Pec K18mob2ptshexp, supplied by DSMZ, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli dh5αmcr  (Agilent technologies)
90
Agilent technologies escherichia coli dh5αmcr
In vivo integration assay in <t>E.</t> <t>coli.</t> Two different mating experiments were done to confirm that the His6-tagged CTnDOT Int protein expressed in E. coli had all of the functions necessary to perform site-specific recombination in vivo. In the first mating, the plasmid pattDOT (Cmr, pir dependent, containing CTnDOT attachment site attDOT) was mobilized from the donor E. coli pir+ strain BW19851 into the recipient E. coli pir-deficient strain EM24NR carrying the plasmid pINT101 (Apr). The pINT101 plasmid contains the wild-type int gene (under the control of Ptac promoter) from CTnDOT and one of its chromosomal target sites (attB). The Cmr Rfr Apr transconjugants (rifampin resistance from the recipient strain EM24NR) were derived from the cointegrate plasmid due to site-specific recombination between the attDOT and attB sites. The reaction was mediated by the wild-type Int protein expressed from pINT101 in the recipient. The second mating had the same donor and a recipient that carried pINT201. pINT201 has the same attB site and expresses the His6-tagged Int protein. The Cmr Rfr Apr transconjugants were obtained at a similar frequency (10−4/recipient; see Results).
Escherichia Coli Dh5αmcr, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli jm109  (Promega)
90
Promega escherichia coli jm109
In vivo integration assay in <t>E.</t> <t>coli.</t> Two different mating experiments were done to confirm that the His6-tagged CTnDOT Int protein expressed in E. coli had all of the functions necessary to perform site-specific recombination in vivo. In the first mating, the plasmid pattDOT (Cmr, pir dependent, containing CTnDOT attachment site attDOT) was mobilized from the donor E. coli pir+ strain BW19851 into the recipient E. coli pir-deficient strain EM24NR carrying the plasmid pINT101 (Apr). The pINT101 plasmid contains the wild-type int gene (under the control of Ptac promoter) from CTnDOT and one of its chromosomal target sites (attB). The Cmr Rfr Apr transconjugants (rifampin resistance from the recipient strain EM24NR) were derived from the cointegrate plasmid due to site-specific recombination between the attDOT and attB sites. The reaction was mediated by the wild-type Int protein expressed from pINT101 in the recipient. The second mating had the same donor and a recipient that carried pINT201. pINT201 has the same attB site and expresses the His6-tagged Int protein. The Cmr Rfr Apr transconjugants were obtained at a similar frequency (10−4/recipient; see Results).
Escherichia Coli Jm109, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli dh5αmcr  (Cosmo Bio USA)
90
Cosmo Bio USA escherichia coli dh5αmcr
In vivo integration assay in <t>E.</t> <t>coli.</t> Two different mating experiments were done to confirm that the His6-tagged CTnDOT Int protein expressed in E. coli had all of the functions necessary to perform site-specific recombination in vivo. In the first mating, the plasmid pattDOT (Cmr, pir dependent, containing CTnDOT attachment site attDOT) was mobilized from the donor E. coli pir+ strain BW19851 into the recipient E. coli pir-deficient strain EM24NR carrying the plasmid pINT101 (Apr). The pINT101 plasmid contains the wild-type int gene (under the control of Ptac promoter) from CTnDOT and one of its chromosomal target sites (attB). The Cmr Rfr Apr transconjugants (rifampin resistance from the recipient strain EM24NR) were derived from the cointegrate plasmid due to site-specific recombination between the attDOT and attB sites. The reaction was mediated by the wild-type Int protein expressed from pINT101 in the recipient. The second mating had the same donor and a recipient that carried pINT201. pINT201 has the same attB site and expresses the His6-tagged Int protein. The Cmr Rfr Apr transconjugants were obtained at a similar frequency (10−4/recipient; see Results).
Escherichia Coli Dh5αmcr, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chef genomic dna plug kit  (Bio-Rad)
94
Bio-Rad chef genomic dna plug kit
In vivo integration assay in <t>E.</t> <t>coli.</t> Two different mating experiments were done to confirm that the His6-tagged CTnDOT Int protein expressed in E. coli had all of the functions necessary to perform site-specific recombination in vivo. In the first mating, the plasmid pattDOT (Cmr, pir dependent, containing CTnDOT attachment site attDOT) was mobilized from the donor E. coli pir+ strain BW19851 into the recipient E. coli pir-deficient strain EM24NR carrying the plasmid pINT101 (Apr). The pINT101 plasmid contains the wild-type int gene (under the control of Ptac promoter) from CTnDOT and one of its chromosomal target sites (attB). The Cmr Rfr Apr transconjugants (rifampin resistance from the recipient strain EM24NR) were derived from the cointegrate plasmid due to site-specific recombination between the attDOT and attB sites. The reaction was mediated by the wild-type Int protein expressed from pINT101 in the recipient. The second mating had the same donor and a recipient that carried pINT201. pINT201 has the same attB site and expresses the His6-tagged Int protein. The Cmr Rfr Apr transconjugants were obtained at a similar frequency (10−4/recipient; see Results).
Chef Genomic Dna Plug Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli dh5αmcr/pemmdhaint  (DSMZ)
90
DSMZ escherichia coli dh5αmcr/pemmdhaint
In vivo integration assay in <t>E.</t> <t>coli.</t> Two different mating experiments were done to confirm that the His6-tagged CTnDOT Int protein expressed in E. coli had all of the functions necessary to perform site-specific recombination in vivo. In the first mating, the plasmid pattDOT (Cmr, pir dependent, containing CTnDOT attachment site attDOT) was mobilized from the donor E. coli pir+ strain BW19851 into the recipient E. coli pir-deficient strain EM24NR carrying the plasmid pINT101 (Apr). The pINT101 plasmid contains the wild-type int gene (under the control of Ptac promoter) from CTnDOT and one of its chromosomal target sites (attB). The Cmr Rfr Apr transconjugants (rifampin resistance from the recipient strain EM24NR) were derived from the cointegrate plasmid due to site-specific recombination between the attDOT and attB sites. The reaction was mediated by the wild-type Int protein expressed from pINT101 in the recipient. The second mating had the same donor and a recipient that carried pINT201. pINT201 has the same attB site and expresses the His6-tagged Int protein. The Cmr Rfr Apr transconjugants were obtained at a similar frequency (10−4/recipient; see Results).
Escherichia Coli Dh5αmcr/Pemmdhaint, supplied by DSMZ, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Bacterial strains and plasmids used in this study

Journal:

Article Title: Salmonella typhimurium Encodes a Putative Iron Transport System within the Centisome 63 Pathogenicity Island

doi:

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: DH5αMCR , F − mcrA Δ( mrr-hsdRMS-mcrBC ) , GIBCO BRL.

Techniques: Plasmid Preparation

In vivo integration assay in E. coli. Two different mating experiments were done to confirm that the His6-tagged CTnDOT Int protein expressed in E. coli had all of the functions necessary to perform site-specific recombination in vivo. In the first mating, the plasmid pattDOT (Cmr, pir dependent, containing CTnDOT attachment site attDOT) was mobilized from the donor E. coli pir+ strain BW19851 into the recipient E. coli pir-deficient strain EM24NR carrying the plasmid pINT101 (Apr). The pINT101 plasmid contains the wild-type int gene (under the control of Ptac promoter) from CTnDOT and one of its chromosomal target sites (attB). The Cmr Rfr Apr transconjugants (rifampin resistance from the recipient strain EM24NR) were derived from the cointegrate plasmid due to site-specific recombination between the attDOT and attB sites. The reaction was mediated by the wild-type Int protein expressed from pINT101 in the recipient. The second mating had the same donor and a recipient that carried pINT201. pINT201 has the same attB site and expresses the His6-tagged Int protein. The Cmr Rfr Apr transconjugants were obtained at a similar frequency (10−4/recipient; see Results).

Journal:

Article Title: Development of an In Vitro Integration Assay for the Bacteroides Conjugative Transposon CTnDOT

doi: 10.1128/JB.184.17.4829-4837.2002

Figure Lengend Snippet: In vivo integration assay in E. coli. Two different mating experiments were done to confirm that the His6-tagged CTnDOT Int protein expressed in E. coli had all of the functions necessary to perform site-specific recombination in vivo. In the first mating, the plasmid pattDOT (Cmr, pir dependent, containing CTnDOT attachment site attDOT) was mobilized from the donor E. coli pir+ strain BW19851 into the recipient E. coli pir-deficient strain EM24NR carrying the plasmid pINT101 (Apr). The pINT101 plasmid contains the wild-type int gene (under the control of Ptac promoter) from CTnDOT and one of its chromosomal target sites (attB). The Cmr Rfr Apr transconjugants (rifampin resistance from the recipient strain EM24NR) were derived from the cointegrate plasmid due to site-specific recombination between the attDOT and attB sites. The reaction was mediated by the wild-type Int protein expressed from pINT101 in the recipient. The second mating had the same donor and a recipient that carried pINT201. pINT201 has the same attB site and expresses the His6-tagged Int protein. The Cmr Rfr Apr transconjugants were obtained at a similar frequency (10−4/recipient; see Results).

Article Snippet: For initial development of the in vitro system, E. coli DH5αMCR (Gibco BRL) was used as the recipient.

Techniques: In Vivo, Plasmid Preparation, Derivative Assay

Frequencies of in vivo integration in E. coli a

Journal:

Article Title: Development of an In Vitro Integration Assay for the Bacteroides Conjugative Transposon CTnDOT

doi: 10.1128/JB.184.17.4829-4837.2002

Figure Lengend Snippet: Frequencies of in vivo integration in E. coli a

Article Snippet: For initial development of the in vitro system, E. coli DH5αMCR (Gibco BRL) was used as the recipient.

Techniques: In Vivo

E. coli host factor IHF can stimulate the in vitro integration reactions. Different concentrations of IHF (lane 1, 0.25 μg; lane 2, 0.125 μg; lane 3, 0.063 μg; lane 4, 0.032 μg; lane 5, 0.016 μg; lane 6, 0.008 μg; lane 7, 0.004 μg) were used in each reaction mixture. The products from the reaction mixtures were digested by EcoRI and BamHI. The 1.8-kb DNA substrate is the digested DNA fragment containing attDOT from the plasmid pattDOT. The 5.3-kb integration product (lanes 1 to 5) is the digested DNA fragment containing attDOT from the final reaction product cointegrate plasmid. The integration product is not seen when the concentration of IHF is too low (<0.008 μg/reaction mixture; lanes 6 and 7). Lane 8, molecular size markers.

Journal:

Article Title: Development of an In Vitro Integration Assay for the Bacteroides Conjugative Transposon CTnDOT

doi: 10.1128/JB.184.17.4829-4837.2002

Figure Lengend Snippet: E. coli host factor IHF can stimulate the in vitro integration reactions. Different concentrations of IHF (lane 1, 0.25 μg; lane 2, 0.125 μg; lane 3, 0.063 μg; lane 4, 0.032 μg; lane 5, 0.016 μg; lane 6, 0.008 μg; lane 7, 0.004 μg) were used in each reaction mixture. The products from the reaction mixtures were digested by EcoRI and BamHI. The 1.8-kb DNA substrate is the digested DNA fragment containing attDOT from the plasmid pattDOT. The 5.3-kb integration product (lanes 1 to 5) is the digested DNA fragment containing attDOT from the final reaction product cointegrate plasmid. The integration product is not seen when the concentration of IHF is too low (<0.008 μg/reaction mixture; lanes 6 and 7). Lane 8, molecular size markers.

Article Snippet: For initial development of the in vitro system, E. coli DH5αMCR (Gibco BRL) was used as the recipient.

Techniques: In Vitro, Plasmid Preparation, Concentration Assay