Journal: Frontiers in Immunology

Article Title: Circulating Reelin promotes inflammation and modulates disease activity in acute and long COVID-19 cases

doi: 10.3389/fimmu.2023.1185748

Figure Lengend Snippet: Reelin expression is increased in COVID-19 rodent models and its inhibition dampens the disease severity. (A) Plasma Reelin expression was measured over time by western blotting (A) in hamsters infected by SARS-CoV-2 (n≥6 per time point) and (B) in hACE2 transgenic mice infected by SARS-CoV-2 (n≥3 per time point). (C) hACE2 transgenic mice were injected intraperitoneally with an anti-Reelin antibody (CR-50, 100µg, n=5) or a control IgG (100µg, n=10) twice per week, starting 3 days before infection with SARS-CoV-2. Survival, weight loss, and clinical score from 1 (healthy) to 4 (dead) were recorded every day. *p<0.05, **p<0.01.

Article Snippet: The effect of anti-Reelin treatment for inhibiting SARS-CoV-2 infection in vivo was determined using transgenic mice globally expressing human angiotensin-converting enzyme 2 (hACE2 Tg), AC70 line (Taconic Biosciences).

Techniques: Expressing, Inhibition, Clinical Proteomics, Western Blot, Infection, Transgenic Assay, Injection, Control

Journal: iScience

Article Title: Broad sarbecovirus neutralization by combined memory B cell antibodies to ancestral SARS-CoV-2

doi: 10.1016/j.isci.2024.110354

Figure Lengend Snippet:

Article Snippet: pUNO-1 SARS-CoV-2 Spike (Lambda)-dfur expression vector , InvivoGen , #p1-spike-v9-df.

Techniques: Virus, Recombinant, Electron Microscopy, Staining, Mutagenesis, Silver Staining, Plasmid Preparation, Expressing, Software, Membrane, Binding Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Imaging, Microscopy, Transmission Assay, Microplate Reader Absorbance Measurement

Journal: Journal of Pharmaceutical Analysis

Article Title: Gliquidone alleviates DSS-induced ulcerative colitis in rats by targeting carnitine palmitoyltransferase 1A

doi: 10.1016/j.jpha.2025.101409

Figure Lengend Snippet: Gliquidone (Glq) attenuate intestinal inflammation in vitro . (A, B) The Ramachandran plot of carnitine palmitoyltransferase 1A (CPT1A) (A) and the sequences of CPT1A structure (B). (C–E) Glq showed no cytotoxicity (C) and inhibited CPT1A expression at test concentrations in THP-1 cells (D) and CCD 841 CoN cells (E). (F) Retention behavior of Glq on CPT1A/mitochondrial membrane chromatography (MMC) columns. (G–J) Glq inhibited intercellular adhesion molecule-1 (ICAM-1) expression in CCD 841 CoN cells (G) and inhibited the release of interleukin-6 (IL-6) (H), interleukin-1 beta (IL-1β) (I), and tumor necrosis factor-alpha (TNF-α) (J) in THP-1 cells. (K) The expression of NOD-like receptor thermal protein domain associated protein 3 (NLRP3) and nuclear factor kappa-B (NF-κB p65) in THP-1 cells. D: ∗∗∗ P <0.001, ∗∗∗∗ P <0.0001 compared to the PAM+LPS group, ## P <0.01 compared to the PMA group; E, G: ∗∗ P <0.01, ∗∗∗∗ P <0.0001 compared to the TNF-α group, ## P <0.01, #### P <0.0001 compared to the Control group; H–K: ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001, ∗∗∗∗ P <0.0001 compared to the LPS group, # P <0.05, #### P <0.0001 compared to the Control group. ETO: etomoxir; PMA: phorbol-12-myristate-13-acetate; LPS: lipopolysaccharides.

Article Snippet: Rats were randomly divided into three groups ( n = 8): Control, DSS, and DSS + gliquidone (2.5 mg/kg) (MCE, Princeton, NJ, USA).

Techniques: In Vitro, Expressing, Membrane, Chromatography, Control

Journal: Journal of Pharmaceutical Analysis

Article Title: Gliquidone alleviates DSS-induced ulcerative colitis in rats by targeting carnitine palmitoyltransferase 1A

doi: 10.1016/j.jpha.2025.101409

Figure Lengend Snippet: Analysis of bonding mode of gliquidone with carnitine palmitoyltransferase 1A (CPT1A).

Article Snippet: Rats were randomly divided into three groups ( n = 8): Control, DSS, and DSS + gliquidone (2.5 mg/kg) (MCE, Princeton, NJ, USA).

Techniques:

Journal: Journal of Pharmaceutical Analysis

Article Title: Gliquidone alleviates DSS-induced ulcerative colitis in rats by targeting carnitine palmitoyltransferase 1A

doi: 10.1016/j.jpha.2025.101409

Figure Lengend Snippet: Gliquidone (Glq) attenuates intestinal injury in vivo. (A) Changes in body weight over the experimental period. (B) Disease activity index (DAI) score. (C) Colon length. (D–F) Inflammatory factors including interleukin-1 beta (IL-1β) (D), interleukin-6 (IL-6) (E) and tumor necrosis factor-alpha (TNF-α) (F) measured in the colon. (G) Intercellular adhesion molecule-1 (ICAM-1) expression in the colon. (H, I) Concentration of long-chain acylcarnitine (LCACs) (H) and long-chain fatty acids (LCFAs) (I) measured in colons. (J, K) Carnitine palmitoyltransferase 1A (CPT1A) (J) and NOD-like receptor thermal protein domain associated protein 3 (NLRP3) (K) expression in colon. ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001, ∗∗∗∗ P <0.0001 compared to the DSS gruop; # P <0.05, ## P <0.01, ### P <0.001, #### P <0.0001 compared to the Control group. DSS: dextran sulfate sodium; C14: myristoyl carnitine; C16: palmityl carnitine; C18: stearoyl carnitine; F14: myristic acid; F16: palmitic acid; F18: stearic acid.

Article Snippet: Rats were randomly divided into three groups ( n = 8): Control, DSS, and DSS + gliquidone (2.5 mg/kg) (MCE, Princeton, NJ, USA).

Techniques: In Vivo, Activity Assay, Expressing, Concentration Assay, Control