Journal: Frontiers in Physiology

Article Title: Optimising the zebrafish Cre/Lox toolbox. Codon improved iCre, new gateway tools, Cre protein and guidelines

doi: 10.3389/fphys.2023.1221310

Figure Lengend Snippet: iCre/Lox toolkit components available at Addgene.

Article Snippet: To further validate our material and iCre’s efficiency in transgenic experiments, we recombined 469-pME-iCre with p5E-UBI (5′clone containing the Ubiquitin promoter) into the optimized destination clone 1456-pDEST-miniTol2_R4R2_Cryst-eGFP (Addgene # 171795), and injected this final construct 114-UBI:iCre_crystGFP (Addgene #171797) with tol2 transposase to promote genomic integration ( ; ).

Techniques: Sequencing, In Vivo, Expressing, Plasmid Preparation, Construct, Clone Assay, Cloning, Marker, Control, Ubiquitin Proteomics

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase Reporter Plasmids

doi: 10.1007/978-1-0716-2453-1_32

Figure Lengend Snippet: Summary of plasmids described in this work. For each plasmid, the following categories are indicated: plasmid type (Type), plasmid abbreviation (Abbreviation), plasmid description (Description), plasmid function (Function), vector backbone (Backbone), vector resistance (Resistance), and source, including reference.

Article Snippet: Omega Destination-CMV:ELuc:bGHpA , pColE1 with CMV:ELuc and Omega Level Entry Point , Empty vector with already integrated control luciferase , ColE1 vector , Spectinomycin , Addgene #124528 ( 12 ).

Techniques: Plasmid Preparation, Control, Luciferase, Binding Assay, Multiplex Assay