Journal: eLife

Article Title: AKT isoforms have distinct hippocampal expression and roles in synaptic plasticity

doi: 10.7554/eLife.30640

Figure Lengend Snippet: ( a ) AKT1 and AKT2 isoforms showed decreased phosphorylation after MK2206 incubation, while AZD5363 resulted in hyperphosphorylation (AKT1: MK2206 F (3, 8) =24.80, p=0.0002, AZD F (3, 8) =37.03, p<0.0001; AKT2: MK2206 F (3, 8) =10.19, p<0.0042, AZD F (3, 8) =34.67, p<0.0001), 3 mice/group. No feedback on PDK1 was observed after incubation with AKT inhibitors (MK2206 F (3, 8) =1.665, p>0.05; AZD5363 F (3, 8) =0.636, p>0.05). ( b ) Validation of specificity of AKT1 and AKT2 phospho-antibodies. ( c ) AKT3 immunoprecipitation specifically pulls down AKT3, leaving AKT1 and AKT2 isoforms in the supernatant. Treatment with 30 µM MK2006 or AZD5363 leads to AKT3 dephosphorylation or hyperphosphorylation.

Article Snippet: Other , Brain slice incubation chamber , Scientific Systems Design Inc , BSC1 , .

Techniques: Phospho-proteomics, Incubation, Biomarker Discovery, Immunoprecipitation, De-Phosphorylation Assay

Journal: eLife

Article Title: AKT isoforms have distinct hippocampal expression and roles in synaptic plasticity

doi: 10.7554/eLife.30640

Figure Lengend Snippet: Baseline recordings of field excitatory postsynaptic potentials (fEPSPs) in CA1 remain stable after incubation with MK2206 or AZD5363 (p>0.05), n = 9–11 slices/group, 4 mice/group respectively, ( F (2,6) =120.3, p<0.0001), 3 mice/group.

Article Snippet: Other , Brain slice incubation chamber , Scientific Systems Design Inc , BSC1 , .

Techniques: Incubation

Journal: eLife

Article Title: AKT isoforms have distinct hippocampal expression and roles in synaptic plasticity

doi: 10.7554/eLife.30640

Figure Lengend Snippet:

Article Snippet: Other , Brain slice incubation chamber , Scientific Systems Design Inc , BSC1 , .

Techniques: Western Blot, Immunostaining, Immunoprecipitation, Protease Inhibitor, Software, Imaging, Slice Preparation, Incubation

Journal: Nature protocols

Article Title: Near-infrared catecholamine nanosensors for high spatiotemporal dopamine imaging

doi: 10.1038/s41596-021-00530-4

Figure Lengend Snippet: a, Schematic representation of the dissection process for the generation of acute coronal brain slices. Red dashed lines represent areas to be cut by using scissors or a vibratome. b Incubation set up to label acute brain slices with nIRCat. c, Microscope perfusion bath set up to image nIRCat-labeled acute brain slices. Coronal brain slices should be placed in the perfusion bath (circle 1) and weighed down by a harp. Acute slices should be placed with the nIRCat-labeled side facing toward the microscope objective.

Article Snippet: Slice incubation chamber (Scientific Systems Design, Inc., BSK4).

Techniques: Dissection, Incubation, Microscopy, Labeling