described21 Search Results


pc 3  (ATCC)
99
ATCC pc 3
Pc 3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transmission electron microscopy tem  (JEOL)
99
JEOL transmission electron microscopy tem
Figure 7. Restoration of cytotoxic activity of CTLs from pediatric patients with NP-C1 by HPbCD. (A) Chromium-51 (51Cr) release assay shows reduced cytotoxicity of CTLs from pediatric patients with NP-C1 at the effector-to-target (E:T) ratios indicated using MDA-MB-231 target cell. Average cytotoxic activity (95% confidence interval shown as shaded gray) of CTLs from 4 HDs. (B) Confocal immunofluorescence <t>microscopy</t> shows GzmB (green)/Prf (magenta) vesicles from an HD and PP4. DAPI is shown in blue. The Diameter of CGs was estimated by computational 3-dimensional re-creation (Imaris vesicles) based on GzmB staining of 2 HD samples and PP4. (C) <t>TEM</t> images show details of activated CTLs (scale bar, 2 mm) and zoomed-in autolysosome-like structures (scale bar, 500 nm). Number of autolysosome-like structures per cell (left), area of autolysosome-like structures per cell (middle), and number of dense-core granules per cell (n 5 73 for HD control and n 5 72 for PP4). (D) Confocal microscopy of
Transmission Electron Microscopy Tem, supplied by JEOL, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gamma interferon (ifn-g  (Becton Dickinson)
90
Becton Dickinson gamma interferon (ifn-g
Figure 7. Restoration of cytotoxic activity of CTLs from pediatric patients with NP-C1 by HPbCD. (A) Chromium-51 (51Cr) release assay shows reduced cytotoxicity of CTLs from pediatric patients with NP-C1 at the effector-to-target (E:T) ratios indicated using MDA-MB-231 target cell. Average cytotoxic activity (95% confidence interval shown as shaded gray) of CTLs from 4 HDs. (B) Confocal immunofluorescence <t>microscopy</t> shows GzmB (green)/Prf (magenta) vesicles from an HD and PP4. DAPI is shown in blue. The Diameter of CGs was estimated by computational 3-dimensional re-creation (Imaris vesicles) based on GzmB staining of 2 HD samples and PP4. (C) <t>TEM</t> images show details of activated CTLs (scale bar, 2 mm) and zoomed-in autolysosome-like structures (scale bar, 500 nm). Number of autolysosome-like structures per cell (left), area of autolysosome-like structures per cell (middle), and number of dense-core granules per cell (n 5 73 for HD control and n 5 72 for PP4). (D) Confocal microscopy of
Gamma Interferon (Ifn G, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtor  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc mtor
Figure 7. Restoration of cytotoxic activity of CTLs from pediatric patients with NP-C1 by HPbCD. (A) Chromium-51 (51Cr) release assay shows reduced cytotoxicity of CTLs from pediatric patients with NP-C1 at the effector-to-target (E:T) ratios indicated using MDA-MB-231 target cell. Average cytotoxic activity (95% confidence interval shown as shaded gray) of CTLs from 4 HDs. (B) Confocal immunofluorescence <t>microscopy</t> shows GzmB (green)/Prf (magenta) vesicles from an HD and PP4. DAPI is shown in blue. The Diameter of CGs was estimated by computational 3-dimensional re-creation (Imaris vesicles) based on GzmB staining of 2 HD samples and PP4. (C) <t>TEM</t> images show details of activated CTLs (scale bar, 2 mm) and zoomed-in autolysosome-like structures (scale bar, 500 nm). Number of autolysosome-like structures per cell (left), area of autolysosome-like structures per cell (middle), and number of dense-core granules per cell (n 5 73 for HD control and n 5 72 for PP4). (D) Confocal microscopy of
Mtor, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti p65  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc anti p65
Figure 7. Restoration of cytotoxic activity of CTLs from pediatric patients with NP-C1 by HPbCD. (A) Chromium-51 (51Cr) release assay shows reduced cytotoxicity of CTLs from pediatric patients with NP-C1 at the effector-to-target (E:T) ratios indicated using MDA-MB-231 target cell. Average cytotoxic activity (95% confidence interval shown as shaded gray) of CTLs from 4 HDs. (B) Confocal immunofluorescence <t>microscopy</t> shows GzmB (green)/Prf (magenta) vesicles from an HD and PP4. DAPI is shown in blue. The Diameter of CGs was estimated by computational 3-dimensional re-creation (Imaris vesicles) based on GzmB staining of 2 HD samples and PP4. (C) <t>TEM</t> images show details of activated CTLs (scale bar, 2 mm) and zoomed-in autolysosome-like structures (scale bar, 500 nm). Number of autolysosome-like structures per cell (left), area of autolysosome-like structures per cell (middle), and number of dense-core granules per cell (n 5 73 for HD control and n 5 72 for PP4). (D) Confocal microscopy of
Anti P65, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti fscn1 mouse monoclonal  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology anti fscn1 mouse monoclonal
Figure 7. Restoration of cytotoxic activity of CTLs from pediatric patients with NP-C1 by HPbCD. (A) Chromium-51 (51Cr) release assay shows reduced cytotoxicity of CTLs from pediatric patients with NP-C1 at the effector-to-target (E:T) ratios indicated using MDA-MB-231 target cell. Average cytotoxic activity (95% confidence interval shown as shaded gray) of CTLs from 4 HDs. (B) Confocal immunofluorescence <t>microscopy</t> shows GzmB (green)/Prf (magenta) vesicles from an HD and PP4. DAPI is shown in blue. The Diameter of CGs was estimated by computational 3-dimensional re-creation (Imaris vesicles) based on GzmB staining of 2 HD samples and PP4. (C) <t>TEM</t> images show details of activated CTLs (scale bar, 2 mm) and zoomed-in autolysosome-like structures (scale bar, 500 nm). Number of autolysosome-like structures per cell (left), area of autolysosome-like structures per cell (middle), and number of dense-core granules per cell (n 5 73 for HD control and n 5 72 for PP4). (D) Confocal microscopy of
Anti Fscn1 Mouse Monoclonal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chitosan  (Valiant Co Ltd)
94
Valiant Co Ltd chitosan
Figure 7. Restoration of cytotoxic activity of CTLs from pediatric patients with NP-C1 by HPbCD. (A) Chromium-51 (51Cr) release assay shows reduced cytotoxicity of CTLs from pediatric patients with NP-C1 at the effector-to-target (E:T) ratios indicated using MDA-MB-231 target cell. Average cytotoxic activity (95% confidence interval shown as shaded gray) of CTLs from 4 HDs. (B) Confocal immunofluorescence <t>microscopy</t> shows GzmB (green)/Prf (magenta) vesicles from an HD and PP4. DAPI is shown in blue. The Diameter of CGs was estimated by computational 3-dimensional re-creation (Imaris vesicles) based on GzmB staining of 2 HD samples and PP4. (C) <t>TEM</t> images show details of activated CTLs (scale bar, 2 mm) and zoomed-in autolysosome-like structures (scale bar, 500 nm). Number of autolysosome-like structures per cell (left), area of autolysosome-like structures per cell (middle), and number of dense-core granules per cell (n 5 73 for HD control and n 5 72 for PP4). (D) Confocal microscopy of
Chitosan, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rpmi 1640  (GE Healthcare)
94
GE Healthcare rpmi 1640
Figure 7. Restoration of cytotoxic activity of CTLs from pediatric patients with NP-C1 by HPbCD. (A) Chromium-51 (51Cr) release assay shows reduced cytotoxicity of CTLs from pediatric patients with NP-C1 at the effector-to-target (E:T) ratios indicated using MDA-MB-231 target cell. Average cytotoxic activity (95% confidence interval shown as shaded gray) of CTLs from 4 HDs. (B) Confocal immunofluorescence <t>microscopy</t> shows GzmB (green)/Prf (magenta) vesicles from an HD and PP4. DAPI is shown in blue. The Diameter of CGs was estimated by computational 3-dimensional re-creation (Imaris vesicles) based on GzmB staining of 2 HD samples and PP4. (C) <t>TEM</t> images show details of activated CTLs (scale bar, 2 mm) and zoomed-in autolysosome-like structures (scale bar, 500 nm). Number of autolysosome-like structures per cell (left), area of autolysosome-like structures per cell (middle), and number of dense-core granules per cell (n 5 73 for HD control and n 5 72 for PP4). (D) Confocal microscopy of
Rpmi 1640, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microscope zeiss kinevo blue 400  (Carl Zeiss)
90
Carl Zeiss microscope zeiss kinevo blue 400
Figure 7. Restoration of cytotoxic activity of CTLs from pediatric patients with NP-C1 by HPbCD. (A) Chromium-51 (51Cr) release assay shows reduced cytotoxicity of CTLs from pediatric patients with NP-C1 at the effector-to-target (E:T) ratios indicated using MDA-MB-231 target cell. Average cytotoxic activity (95% confidence interval shown as shaded gray) of CTLs from 4 HDs. (B) Confocal immunofluorescence <t>microscopy</t> shows GzmB (green)/Prf (magenta) vesicles from an HD and PP4. DAPI is shown in blue. The Diameter of CGs was estimated by computational 3-dimensional re-creation (Imaris vesicles) based on GzmB staining of 2 HD samples and PP4. (C) <t>TEM</t> images show details of activated CTLs (scale bar, 2 mm) and zoomed-in autolysosome-like structures (scale bar, 500 nm). Number of autolysosome-like structures per cell (left), area of autolysosome-like structures per cell (middle), and number of dense-core granules per cell (n 5 73 for HD control and n 5 72 for PP4). (D) Confocal microscopy of
Microscope Zeiss Kinevo Blue 400, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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described 21 polyclonal rabbit anti human dkk1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology described 21 polyclonal rabbit anti human dkk1
Figure 1. Representative images of Dickkopf-related protein 1 <t>(DKK1),</t> matrix metalloproteinase 9 (MMP9), and vascular endothe- lial growth factor C (VEGF-C) are shown in tissue microarrays by immunochemistry analysis. Histology slides show (A) hematoxy- lin and eosin (HE) staining, no staining (DKK1), low staining (DKK1þ), moderate staining (DKK1þþ), strong staining (DKK1þþþ), and weak staining (DKK1) for DKK1 in peritumoral tissue (a, b, c, d, e, f, respectively) (original magnification, 400, bar ¼ 100 lm); (B) strong staining for DKK1, MMP9, and VEGF-C in case 18 (a, b, c) and weak staining of DKK1, MMP9, and VEGF-C staining in case 9 (d, e, f) (original magnification, 400, bar ¼ 100 lm).
Described 21 Polyclonal Rabbit Anti Human Dkk1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hindiii noti fragment  (TaKaRa)
99
TaKaRa hindiii noti fragment
Figure 1. Representative images of Dickkopf-related protein 1 <t>(DKK1),</t> matrix metalloproteinase 9 (MMP9), and vascular endothe- lial growth factor C (VEGF-C) are shown in tissue microarrays by immunochemistry analysis. Histology slides show (A) hematoxy- lin and eosin (HE) staining, no staining (DKK1), low staining (DKK1þ), moderate staining (DKK1þþ), strong staining (DKK1þþþ), and weak staining (DKK1) for DKK1 in peritumoral tissue (a, b, c, d, e, f, respectively) (original magnification, 400, bar ¼ 100 lm); (B) strong staining for DKK1, MMP9, and VEGF-C in case 18 (a, b, c) and weak staining of DKK1, MMP9, and VEGF-C staining in case 9 (d, e, f) (original magnification, 400, bar ¼ 100 lm).
Hindiii Noti Fragment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sodium dodecyl sulfate polyacrylamide gel electrophoresis  (Bio-Rad)
97
Bio-Rad sodium dodecyl sulfate polyacrylamide gel electrophoresis
Figure 1. Representative images of Dickkopf-related protein 1 <t>(DKK1),</t> matrix metalloproteinase 9 (MMP9), and vascular endothe- lial growth factor C (VEGF-C) are shown in tissue microarrays by immunochemistry analysis. Histology slides show (A) hematoxy- lin and eosin (HE) staining, no staining (DKK1), low staining (DKK1þ), moderate staining (DKK1þþ), strong staining (DKK1þþþ), and weak staining (DKK1) for DKK1 in peritumoral tissue (a, b, c, d, e, f, respectively) (original magnification, 400, bar ¼ 100 lm); (B) strong staining for DKK1, MMP9, and VEGF-C in case 18 (a, b, c) and weak staining of DKK1, MMP9, and VEGF-C staining in case 9 (d, e, f) (original magnification, 400, bar ¼ 100 lm).
Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 7. Restoration of cytotoxic activity of CTLs from pediatric patients with NP-C1 by HPbCD. (A) Chromium-51 (51Cr) release assay shows reduced cytotoxicity of CTLs from pediatric patients with NP-C1 at the effector-to-target (E:T) ratios indicated using MDA-MB-231 target cell. Average cytotoxic activity (95% confidence interval shown as shaded gray) of CTLs from 4 HDs. (B) Confocal immunofluorescence microscopy shows GzmB (green)/Prf (magenta) vesicles from an HD and PP4. DAPI is shown in blue. The Diameter of CGs was estimated by computational 3-dimensional re-creation (Imaris vesicles) based on GzmB staining of 2 HD samples and PP4. (C) TEM images show details of activated CTLs (scale bar, 2 mm) and zoomed-in autolysosome-like structures (scale bar, 500 nm). Number of autolysosome-like structures per cell (left), area of autolysosome-like structures per cell (middle), and number of dense-core granules per cell (n 5 73 for HD control and n 5 72 for PP4). (D) Confocal microscopy of

Journal: Blood

Article Title: Severely impaired CTL killing is a feature of the neurological disorder Niemann-Pick disease type C1.

doi: 10.1182/blood.2021013477

Figure Lengend Snippet: Figure 7. Restoration of cytotoxic activity of CTLs from pediatric patients with NP-C1 by HPbCD. (A) Chromium-51 (51Cr) release assay shows reduced cytotoxicity of CTLs from pediatric patients with NP-C1 at the effector-to-target (E:T) ratios indicated using MDA-MB-231 target cell. Average cytotoxic activity (95% confidence interval shown as shaded gray) of CTLs from 4 HDs. (B) Confocal immunofluorescence microscopy shows GzmB (green)/Prf (magenta) vesicles from an HD and PP4. DAPI is shown in blue. The Diameter of CGs was estimated by computational 3-dimensional re-creation (Imaris vesicles) based on GzmB staining of 2 HD samples and PP4. (C) TEM images show details of activated CTLs (scale bar, 2 mm) and zoomed-in autolysosome-like structures (scale bar, 500 nm). Number of autolysosome-like structures per cell (left), area of autolysosome-like structures per cell (middle), and number of dense-core granules per cell (n 5 73 for HD control and n 5 72 for PP4). (D) Confocal microscopy of

Article Snippet: Volocity 3D Image Analysis software was used to calculate protein and lipid colocalization.28 Time-lapse microscopy was conducted as previously described.21 For transmission electron microscopy (TEM), cells were imaged with a JEOL 1400 Flash electron microscope and quantified with Fiji-ImageJ.29 Detailed settings and analyses are described in the data supplement.

Techniques: Activity Assay, Release Assay, Microscopy, Staining, Control, Confocal Microscopy

Figure 1. Representative images of Dickkopf-related protein 1 (DKK1), matrix metalloproteinase 9 (MMP9), and vascular endothe- lial growth factor C (VEGF-C) are shown in tissue microarrays by immunochemistry analysis. Histology slides show (A) hematoxy- lin and eosin (HE) staining, no staining (DKK1), low staining (DKK1þ), moderate staining (DKK1þþ), strong staining (DKK1þþþ), and weak staining (DKK1) for DKK1 in peritumoral tissue (a, b, c, d, e, f, respectively) (original magnification, 400, bar ¼ 100 lm); (B) strong staining for DKK1, MMP9, and VEGF-C in case 18 (a, b, c) and weak staining of DKK1, MMP9, and VEGF-C staining in case 9 (d, e, f) (original magnification, 400, bar ¼ 100 lm).

Journal: Cancer

Article Title: High expression of Dickkopf-related protein 1 is related to lymphatic metastasis and indicates poor prognosis in intrahepatic cholangiocarcinoma patients after surgery.

doi: 10.1002/cncr.27788

Figure Lengend Snippet: Figure 1. Representative images of Dickkopf-related protein 1 (DKK1), matrix metalloproteinase 9 (MMP9), and vascular endothe- lial growth factor C (VEGF-C) are shown in tissue microarrays by immunochemistry analysis. Histology slides show (A) hematoxy- lin and eosin (HE) staining, no staining (DKK1), low staining (DKK1þ), moderate staining (DKK1þþ), strong staining (DKK1þþþ), and weak staining (DKK1) for DKK1 in peritumoral tissue (a, b, c, d, e, f, respectively) (original magnification, 400, bar ¼ 100 lm); (B) strong staining for DKK1, MMP9, and VEGF-C in case 18 (a, b, c) and weak staining of DKK1, MMP9, and VEGF-C staining in case 9 (d, e, f) (original magnification, 400, bar ¼ 100 lm).

Article Snippet: TMAs were constructed and immunohistochemistry was performed as described.21 Polyclonal rabbit anti-human DKK1 (sc-25516, 1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, Calif), polyclonal rabbit anti-human VEGF-C antibody (AP2042d, 1:50 dilution; Abgent, San Diego, Calif), and polyclonal rabbit anti-human MMP9 antibody (AP6214a, 1:50 dilution; Abgent) were used to detect the expression of DKK1, VEGF-C, and MMP9, respectively.

Techniques: Staining

Figure 2. Kaplan-Meier analysis for overall survival (OS) and time to recurrence (TTR) is shown in 138 intrahepatic cholangiocarci- noma (ICC) patients based on Dickkopf-related protein 1 (DKK1) expression. (A, B) Compared with the DKK1-positive (DKK1þ) group, the TTR and OS were significantly higher in the DKK1-negative (DKK1) group; (C, D) The prognostic significances of DKK1 for OS and TTR are shown in patients with TNM stage IþII.

Journal: Cancer

Article Title: High expression of Dickkopf-related protein 1 is related to lymphatic metastasis and indicates poor prognosis in intrahepatic cholangiocarcinoma patients after surgery.

doi: 10.1002/cncr.27788

Figure Lengend Snippet: Figure 2. Kaplan-Meier analysis for overall survival (OS) and time to recurrence (TTR) is shown in 138 intrahepatic cholangiocarci- noma (ICC) patients based on Dickkopf-related protein 1 (DKK1) expression. (A, B) Compared with the DKK1-positive (DKK1þ) group, the TTR and OS were significantly higher in the DKK1-negative (DKK1) group; (C, D) The prognostic significances of DKK1 for OS and TTR are shown in patients with TNM stage IþII.

Article Snippet: TMAs were constructed and immunohistochemistry was performed as described.21 Polyclonal rabbit anti-human DKK1 (sc-25516, 1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, Calif), polyclonal rabbit anti-human VEGF-C antibody (AP2042d, 1:50 dilution; Abgent, San Diego, Calif), and polyclonal rabbit anti-human MMP9 antibody (AP6214a, 1:50 dilution; Abgent) were used to detect the expression of DKK1, VEGF-C, and MMP9, respectively.

Techniques: Expressing

Figure 3. Kaplan-Meier analysis for Dickkopf-related protein 1 (DKK1) is shown in other intrahepatic cholangiocarcinoma sub- groups. (A, B) Prognostic role of DKK1 in patients without hilar lymph node metastasis, (C, D) without vascular invasion, (E, F) with a single tumor, and (G, H) with well-differentiated tumor.

Journal: Cancer

Article Title: High expression of Dickkopf-related protein 1 is related to lymphatic metastasis and indicates poor prognosis in intrahepatic cholangiocarcinoma patients after surgery.

doi: 10.1002/cncr.27788

Figure Lengend Snippet: Figure 3. Kaplan-Meier analysis for Dickkopf-related protein 1 (DKK1) is shown in other intrahepatic cholangiocarcinoma sub- groups. (A, B) Prognostic role of DKK1 in patients without hilar lymph node metastasis, (C, D) without vascular invasion, (E, F) with a single tumor, and (G, H) with well-differentiated tumor.

Article Snippet: TMAs were constructed and immunohistochemistry was performed as described.21 Polyclonal rabbit anti-human DKK1 (sc-25516, 1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, Calif), polyclonal rabbit anti-human VEGF-C antibody (AP2042d, 1:50 dilution; Abgent, San Diego, Calif), and polyclonal rabbit anti-human MMP9 antibody (AP6214a, 1:50 dilution; Abgent) were used to detect the expression of DKK1, VEGF-C, and MMP9, respectively.

Techniques:

Figure 4. Expression of DKK1 is shown in cell lines and clinical specimens. (A) DKK1 expression in HCCC-9810 and IBEpiC cells were detected by immunofluorescence assay, western blot, and qRT-PCR analysis. The ELISA showed DKK1 level in HCCC-9810 supernatant was significantly higher than that in IBEpiC cells. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a housekeeping gene; (B) DKK1 messenger RNA (mRNA) expression in intrahepatic cholangiocarci- noma (ICC) tumor tissues was higher than that in peritumoral tissues; (C) The mRNA expression levels of DKK1 in ICC tis- sues of patients suffering ICC recurrence (16 of 36) were higher than in those without recurrence; (D, E) DKK1 protein expression in ICC patients with no recurrence, in those with recurrence, and in peritumoral tissues was detected by west- ern blot analysis; (F) Serum DKK1 level was significantly higher in ICC patients and receiver operating characteristic (ROC) curve of DKK1 for diagnosis; (G) The mRNA expression of DKK1 was positively related to MMP9 and VEGF-C expression.

Journal: Cancer

Article Title: High expression of Dickkopf-related protein 1 is related to lymphatic metastasis and indicates poor prognosis in intrahepatic cholangiocarcinoma patients after surgery.

doi: 10.1002/cncr.27788

Figure Lengend Snippet: Figure 4. Expression of DKK1 is shown in cell lines and clinical specimens. (A) DKK1 expression in HCCC-9810 and IBEpiC cells were detected by immunofluorescence assay, western blot, and qRT-PCR analysis. The ELISA showed DKK1 level in HCCC-9810 supernatant was significantly higher than that in IBEpiC cells. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a housekeeping gene; (B) DKK1 messenger RNA (mRNA) expression in intrahepatic cholangiocarci- noma (ICC) tumor tissues was higher than that in peritumoral tissues; (C) The mRNA expression levels of DKK1 in ICC tis- sues of patients suffering ICC recurrence (16 of 36) were higher than in those without recurrence; (D, E) DKK1 protein expression in ICC patients with no recurrence, in those with recurrence, and in peritumoral tissues was detected by west- ern blot analysis; (F) Serum DKK1 level was significantly higher in ICC patients and receiver operating characteristic (ROC) curve of DKK1 for diagnosis; (G) The mRNA expression of DKK1 was positively related to MMP9 and VEGF-C expression.

Article Snippet: TMAs were constructed and immunohistochemistry was performed as described.21 Polyclonal rabbit anti-human DKK1 (sc-25516, 1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, Calif), polyclonal rabbit anti-human VEGF-C antibody (AP2042d, 1:50 dilution; Abgent, San Diego, Calif), and polyclonal rabbit anti-human MMP9 antibody (AP6214a, 1:50 dilution; Abgent) were used to detect the expression of DKK1, VEGF-C, and MMP9, respectively.

Techniques: Expressing, Immunofluorescence, Western Blot, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Biomarker Discovery

Figure 5. Functional analysis after DKK1 small interfering RNA (siRNA) in HCCC-9810 cells. (A) DKK1 expression was decreased significantly after siRNA for 48 hours, according to qRT-PCR, and 72 hours, according to western blot analysis; (B) The concen- tration of DKK1 in cell supernatants was also inhibited by siRNA; (C) Transwell Matrigel invasion assays showed decreased DKK1 expression were accompanied by impairment in the invasiveness of HCCC-9810 cells (original magnification 50, bar ¼ 100 lm); (D) Monolayers of DKK1 siRNA-treated or negative control were abraded and then monitored at 0, 24, and 48 hours for wound healing test. *P < .05. The cleaned areas were calculated by Image Pro Plus software 6.0 (original magnification 50, bar ¼ 100 lm); (E) Cell proliferation after DKK1 siRNA knockdown was detected by the CCK-8 assay; (F) The mRNA level of VEGF-C and MMP9 were significantly down-regulated after DKK1 knockdown.

Journal: Cancer

Article Title: High expression of Dickkopf-related protein 1 is related to lymphatic metastasis and indicates poor prognosis in intrahepatic cholangiocarcinoma patients after surgery.

doi: 10.1002/cncr.27788

Figure Lengend Snippet: Figure 5. Functional analysis after DKK1 small interfering RNA (siRNA) in HCCC-9810 cells. (A) DKK1 expression was decreased significantly after siRNA for 48 hours, according to qRT-PCR, and 72 hours, according to western blot analysis; (B) The concen- tration of DKK1 in cell supernatants was also inhibited by siRNA; (C) Transwell Matrigel invasion assays showed decreased DKK1 expression were accompanied by impairment in the invasiveness of HCCC-9810 cells (original magnification 50, bar ¼ 100 lm); (D) Monolayers of DKK1 siRNA-treated or negative control were abraded and then monitored at 0, 24, and 48 hours for wound healing test. *P < .05. The cleaned areas were calculated by Image Pro Plus software 6.0 (original magnification 50, bar ¼ 100 lm); (E) Cell proliferation after DKK1 siRNA knockdown was detected by the CCK-8 assay; (F) The mRNA level of VEGF-C and MMP9 were significantly down-regulated after DKK1 knockdown.

Article Snippet: TMAs were constructed and immunohistochemistry was performed as described.21 Polyclonal rabbit anti-human DKK1 (sc-25516, 1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, Calif), polyclonal rabbit anti-human VEGF-C antibody (AP2042d, 1:50 dilution; Abgent, San Diego, Calif), and polyclonal rabbit anti-human MMP9 antibody (AP6214a, 1:50 dilution; Abgent) were used to detect the expression of DKK1, VEGF-C, and MMP9, respectively.

Techniques: Functional Assay, Small Interfering RNA, Expressing, Quantitative RT-PCR, Western Blot, Negative Control, Software, Knockdown, CCK-8 Assay