Journal: Journal of Translational Medicine

Article Title: Conjugated bile acids alleviate acute pancreatitis through inhibition of TGR5 and NLRP3 mediated inflammation

doi: 10.1186/s12967-024-05922-0

Figure Lengend Snippet: The decrease of TCDCA may be a key factor in aggravating acute pancreatitis. Serum was collected in caerulein or PBS injection mice at 12 h post caerulein-induced SAP modeling for bile acids quasi-targeted metabolomics ( n = 6). a OPLS-DA scatter plots of serum bile acids in WT or Baat −/− mice of control and b SAP model and the Permutation analysis of it. c Heatmap showing different bile acids in serum of individual animals between WT and Baat −/− mice of control and caerulein-induced SAP model. Univariate analysis of major serum bile acids in WT d and e mice of control and SAP model. Volcano map of comparing the control group with the model group of WT f and Baat −/− g mice. Each dot indicates an individual metabolite. Data are presented as mean ± s.d. The two-sided P values were examined using Student’s t-test for comparison of variables between two groups. One-way ANOVA followed by Tukey’s multiple-comparison test was used for comparison of continuous variables among multiple groups. *: P < .05; **: P < .01; ***: P < .001; ****: P < .0001

Article Snippet: The following reagents were used for this study: caerulein (HY-A0190, MCE); alpha-amylase determination kit (BIOSINO, Beijing, China), LPS from Escherichia coli O55: B5 (Sigma, Germany), Nigericin (N1495, Invitrogen).Cholic acid (81–25-4), Chenodeoxycholic Acid (474–25-9), Taurodeoxycholate sodium salt (1180–95-6), Taurocholic acid (81–24-3), Taurochenodeoxycholic acid (516–35-8), Deoxycholic acid sodium salt (302–95-4), Lithocholic acid (434–13-9), Taurolithocholic acid sodium salt (6042–32-6), Ursodeoxycholic acid (128–13-2) and Glycoursodeoxycholic acid (64,480–66-6) are all from MCE.

Techniques: Injection, Control, Comparison

Journal: Journal of Translational Medicine

Article Title: Conjugated bile acids alleviate acute pancreatitis through inhibition of TGR5 and NLRP3 mediated inflammation

doi: 10.1186/s12967-024-05922-0

Figure Lengend Snippet: Taurine or glycine-conjugated forms of CDCA show stronger anti-inflammatory effects. a LPS-primed BMDMs stimulated with nigericin (2 μM) for 3 h were treated with indicated BAs at 50 μM for 24 h. Relative mRNA expression of Il1b (left), Il6 (mid), and Tnfa (right) in BMDMs treated with 50 μM BAs ( n = 6). b LPS-primed BMDMs stimulated with nigericin (2 μM) for 3 h were treated with CDCA or TCDCA at 5–100 μM for 24 h. Relative mRNA expression of Il1b (left) and Il6 (right)in BMDMs treated with BAs of gradient concentration from 5 to 100 μM ( n = 6). c LPS-primed Peritoneal macrophages stimulated with nigericin (2 μM) for 3 h were treated with CDCA or TCDCA at 5–100 μM for 24 h. Relative mRNA expression of Il1b (left) and Il6 (right) in peritoneal macrophages treated with BAs of gradient concentration from 5 to 100 μM ( n = 6). Data are presented as mean ± s.d. The two-sided P values were examined using Student’s t-test for comparison of variables between two groups. One-way ANOVA followed by Tukey’s multiple-comparison test was used for comparison of continuous variables among multiple groups. *: P < .05; **: P < .01; ***: P < .001; ****: P < .0001

Article Snippet: The following reagents were used for this study: caerulein (HY-A0190, MCE); alpha-amylase determination kit (BIOSINO, Beijing, China), LPS from Escherichia coli O55: B5 (Sigma, Germany), Nigericin (N1495, Invitrogen).Cholic acid (81–25-4), Chenodeoxycholic Acid (474–25-9), Taurodeoxycholate sodium salt (1180–95-6), Taurocholic acid (81–24-3), Taurochenodeoxycholic acid (516–35-8), Deoxycholic acid sodium salt (302–95-4), Lithocholic acid (434–13-9), Taurolithocholic acid sodium salt (6042–32-6), Ursodeoxycholic acid (128–13-2) and Glycoursodeoxycholic acid (64,480–66-6) are all from MCE.

Techniques: Expressing, Concentration Assay, Comparison

Journal: Journal of Translational Medicine

Article Title: Conjugated bile acids alleviate acute pancreatitis through inhibition of TGR5 and NLRP3 mediated inflammation

doi: 10.1186/s12967-024-05922-0

Figure Lengend Snippet: TCDCA treatment alleviated severe acute pancreatitis in mice. a - g 3 mM TCDCA was added to the drinking water for 4 weeks before caerulein injection SAP models. Samples were at 12 h post caerulein-induced SAP modeling ( n = 6). a Schematic illustration of experiments in complement of TCDCA and SAP models induced by caerulein injection. b Serum amylase activity and c serum lipase activity of SAP mice. n = 3 biologically independent samples. d Pathological sections and e pancreatic pathology scores from mice from a . Histopathological changes were scored by HE staining. Blue arrows indicate acinar necrosis, pink arrows indicate inflammatory cell infiltration, and black arrows indicate edema. n = 3 biologically independent samples. f Serum expression (ELISA) of IL-1β (left), IL-6 (mid) and TNF-α (right) in WT or Baat −/− SAP mice. n = 3 biologically independent samples. g Relative mRNA expression of Il1b (left) and Il6 (right) in the pancreas in WT or Baat −/− SAP mice. Fold change is relative to respective mock mice. n = 3 biologically independent samples. Data are presented as mean ± s.d. The two-sided P values were examined using Student’s t-test for comparison of variables between two groups. One-way ANOVA followed by Tukey’s multiple-comparison test was used for comparison of continuous variables among multiple groups. *: P < .05; **: P < .01; ***: P < .001; ****: P < .0001

Article Snippet: The following reagents were used for this study: caerulein (HY-A0190, MCE); alpha-amylase determination kit (BIOSINO, Beijing, China), LPS from Escherichia coli O55: B5 (Sigma, Germany), Nigericin (N1495, Invitrogen).Cholic acid (81–25-4), Chenodeoxycholic Acid (474–25-9), Taurodeoxycholate sodium salt (1180–95-6), Taurocholic acid (81–24-3), Taurochenodeoxycholic acid (516–35-8), Deoxycholic acid sodium salt (302–95-4), Lithocholic acid (434–13-9), Taurolithocholic acid sodium salt (6042–32-6), Ursodeoxycholic acid (128–13-2) and Glycoursodeoxycholic acid (64,480–66-6) are all from MCE.

Techniques: Injection, Activity Assay, Staining, Expressing, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Journal of Translational Medicine

Article Title: Conjugated bile acids alleviate acute pancreatitis through inhibition of TGR5 and NLRP3 mediated inflammation

doi: 10.1186/s12967-024-05922-0

Figure Lengend Snippet: TCDCA alleviates pancreatitis via TGR5 and NLRP3 signaling. a - g 3 mM TCDCA was added to the drinking water for 4 weeks before caerulein injection SAP models. Samples were at 12 h post caerulein-induced SAP modeling ( n = 6). a Representative western blot showing expression of TGR5 and NLRP3 signaling pathway-related molecules (NLRP3, pro-CASP1 and CASP1 p10) in the pancreas. Densitometric analysis of band intensity was performed using ImageJ software. The grayscale intensity of the bands was normalized and the results are expressed as a fold change relative to the control group. b Pancreatic pathological sections and c pathology scores from different species (WT, TGR5 −/− and NLRP3 −/− ). Histopathological changes were scored by HE staining. Blue arrows indicate acinar necrosis, pink arrows indicate inflammatory cell infiltration, and black arrows indicate edema. n = 3 biologically independent samples. d Serum amylase activity and e serum lipase activity of different species mice in SAP model. n = 3 biologically independent samples. f Serum expression (ELISA) of IL-1β (left), IL-6 (mid) and TNF-α (right) in WT, TGR5 −/− and NLRP3 −/− SAP mice. n = 3 biologically independent samples. g Relative mRNA expression of Il1b (left) and Il6 (right) in the pancreas in WT, TGR5 −/− and NLRP3 −/− SAP mice. Fold change is relative to respective mock mice. n = 3 biologically independent samples. Data are presented as mean ± s.d. The two-sided P values were examined using Student’s t-test for comparison of variables between two groups. One-way ANOVA followed by Tukey’s multiple-comparison test was used for comparison of continuous variables among multiple groups. *: P < .05; **: P < .01; ***: P < .001; ****: P < .0001. Representative images of three independent replicates were shown in immunoblotting analysis

Article Snippet: The following reagents were used for this study: caerulein (HY-A0190, MCE); alpha-amylase determination kit (BIOSINO, Beijing, China), LPS from Escherichia coli O55: B5 (Sigma, Germany), Nigericin (N1495, Invitrogen).Cholic acid (81–25-4), Chenodeoxycholic Acid (474–25-9), Taurodeoxycholate sodium salt (1180–95-6), Taurocholic acid (81–24-3), Taurochenodeoxycholic acid (516–35-8), Deoxycholic acid sodium salt (302–95-4), Lithocholic acid (434–13-9), Taurolithocholic acid sodium salt (6042–32-6), Ursodeoxycholic acid (128–13-2) and Glycoursodeoxycholic acid (64,480–66-6) are all from MCE.

Techniques: Injection, Western Blot, Expressing, Software, Control, Staining, Activity Assay, Enzyme-linked Immunosorbent Assay, Comparison

Journal: Pharmaceuticals

Article Title: Novel Siprulina platensis Bilosomes for Combating UVB Induced Skin Damage

doi: 10.3390/ph16010036

Figure Lengend Snippet: Composition of empty and SPR-loaded bilosomes SPR-BS prepared by thin film hydration method.

Article Snippet: Sodium deoxycholate was bought from Chem-Impex International, Inc. (Wood Dale, IL, USA), and Cholesterol with a molecular weight of 386.654 g/mol was gifted from The Nile for Pharmaceuticals and Chemical Industries (Cairo, Egypt).

Techniques: