deoxycholate Search Results


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  • 99
    Thermo Fisher sodium deoxycholate
    a) In vivo release of 4HPR from CR PLGA millicylinders vs . that from water soluble matrix PVA/sucrose implants with and without solubilizers. The PLGA implants contained 20% 4HPR + 15% MgCO 3 + 1% PVP + 20% NaDC or CaDC, while the PVA/sucrose implants contained either 20% 4HPR or additional solubilizer and crystallization inhibitor (20% NaDC + 1% PVP). b) Implant images prior to harvesting from rat SC tissue on day 28 (yellow represents 4HPR). C) In vivo release of sodium and calcium <t>deoxycholate</t> (DC − ) from PLGA millicylinders. ( mean ± SE, n = 3).
    Sodium Deoxycholate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3607 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 3607 article reviews
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    99
    Millipore deoxycholate
    Comparison of the change in the cyclin A protein in human colonocytes and HCT116 colon cancer cells after incubation for 24 hours with deoxycholic acid based on a Western blot analysis. ERK, extracellular signal-regulated kinase.
    Deoxycholate, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2798 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxycholate/product/Millipore
    Average 99 stars, based on 2798 article reviews
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    93
    Fisher Scientific sodium deoxycholate
    Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium <t>deoxycholate;</t> C—chitosan-coated (0.5 mg/mL).
    Sodium Deoxycholate, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 144 article reviews
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    92
    Amresco deoxycholate
    Weighted chemical shift difference (Δδ HN ) of the 1 H and 15 N resonances of SipD 39–343 when titrated with (A) <t>deoxycholate,</t> (B) taurodeoxycholate and (C) chenodeoxycholate (Δδ HN = [½ (δ H 2 + 1/25δ
    Deoxycholate, supplied by Amresco, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxycholate/product/Amresco
    Average 92 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
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    99
    Roche sodium deoxycholate
    MAGE-A11 interacts with p107 and Rb but not p130. pCMV-FLAG (−), pCMV-FLAG-MAGE, or FLAG-MAGE-(112–429) (5 μg) was expressed in COS1 cells with 5 μg of CMV-p107 ( A ), pCMV-hRb ( B ), or pcDNA3-p130 ( C ). Cells were incubated for 24 h in serum-free medium containing 0.1 μg/ml EGF and harvested in immunoprecipitation lysis buffer without <t>deoxycholate</t> or glycerol. Cell extracts (50 μg of protein/lane, right ) and immunoprecipitates ( IP , left ) were probed using p107, Rb, p130, and MAGE-A11 antibodies. Arrows , p107, Rb, p130, FLAG-MAGE, and IgG.
    Sodium Deoxycholate, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 16461 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    FUJIFILM sodium deoxycholate
    Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium <t>deoxycholate</t> complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.
    Sodium Deoxycholate, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 93/100, based on 183 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    honeywell international deoxycholic acid
    Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium <t>deoxycholate</t> complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.
    Deoxycholic Acid, supplied by honeywell international, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Valiant sodium deoxycholate
    Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium <t>deoxycholate</t> complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.
    Sodium Deoxycholate, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium deoxycholate/product/Valiant
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    99
    Millipore deoxycholic acid dca
    Bile acid metabolism in animals treated with vehicle or TCDF (24 μg/kg). Abbreviations: CA, cholic acid; CDCA, chenodeoxycholic acid; <t>DCA,</t> <t>deoxycholic</t> acid; G, glycine-conjugated; LCA, lithocholic acid; MCA, muricholic acid; NS, not significant; T, taurine-conjugated species; UDCA, ursodeoxycholic acid. Quantification of specific bile acids levels in intestinal tissue ( A,B ) and feces ( C,D ) throughout the enterohepatic circulation of Ahr +/+ mice. ( E ) Quantification of total bile acids in liver, intestine, cecum, and feces of Ahr +/+ mice; the bile acid profile in the small intestine shows the data from jejunum segment. qPCR analysis of Fgf15 , Fxr, and Shp mRNAs in the ileum ( F ) and Fxr and Shp mRNA expression in the liver ( G ) of Ahr +/+ and Ahr –/– mice. ( H ) qPCR analysis of Cyp7a1 , Cyp8b1 , Cyp27a1 , Akr1d1, and Cyp7b1 mRNAs in the liver of Ahr +/+ mice. ( I – K ) mRNA encoding bile acid transporters involved in taurine biosynthesis and bile acid conjugation in the ileum ( I ), and mRNA encoding bile acid transporters in the distal liver ( J ) and ileum ( K ) of vehicle- and TCDF-treated Ahr +/+ mice. Data are presented as mean ± SD; n = 6/group. See also Supplemental Material, Tables S1 and S2. * p
    Deoxycholic Acid Dca, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Merck & Co deoxycholic acid
    Bile salt hydrolase (BSH) enzyme activity assay. Taurodeoxycholic acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of <t>deoxycholic</t> acid formation (* p
    Deoxycholic Acid, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    a) In vivo release of 4HPR from CR PLGA millicylinders vs . that from water soluble matrix PVA/sucrose implants with and without solubilizers. The PLGA implants contained 20% 4HPR + 15% MgCO 3 + 1% PVP + 20% NaDC or CaDC, while the PVA/sucrose implants contained either 20% 4HPR or additional solubilizer and crystallization inhibitor (20% NaDC + 1% PVP). b) Implant images prior to harvesting from rat SC tissue on day 28 (yellow represents 4HPR). C) In vivo release of sodium and calcium deoxycholate (DC − ) from PLGA millicylinders. ( mean ± SE, n = 3).

    Journal: International journal of pharmaceutics

    Article Title: In vivo controlled release of fenretinide from long-acting release depots for chemoprevention of oral squamous cell carcinoma recurrence

    doi: 10.1016/j.ijpharm.2017.11.037

    Figure Lengend Snippet: a) In vivo release of 4HPR from CR PLGA millicylinders vs . that from water soluble matrix PVA/sucrose implants with and without solubilizers. The PLGA implants contained 20% 4HPR + 15% MgCO 3 + 1% PVP + 20% NaDC or CaDC, while the PVA/sucrose implants contained either 20% 4HPR or additional solubilizer and crystallization inhibitor (20% NaDC + 1% PVP). b) Implant images prior to harvesting from rat SC tissue on day 28 (yellow represents 4HPR). C) In vivo release of sodium and calcium deoxycholate (DC − ) from PLGA millicylinders. ( mean ± SE, n = 3).

    Article Snippet: Excipients used were sodium deoxycholate, (NaDC, 99% pure, Acros), polyvinylpyrrolidone (PVP K30, 40 kDa, BASF), hydroxypropyl methylcellulose K4M (HPMC K4M, Dow Chemical, Midland, MI), and β-CD (Sigma-Aldrich).

    Techniques: In Vivo, Crystallization Assay

    Comparison of the change in the cyclin A protein in human colonocytes and HCT116 colon cancer cells after incubation for 24 hours with deoxycholic acid based on a Western blot analysis. ERK, extracellular signal-regulated kinase.

    Journal: Journal of the Korean Society of Coloproctology

    Article Title: Effects of DCA on Cell Cycle Proteins in Colonocytes

    doi: 10.3393/jksc.2010.26.4.254

    Figure Lengend Snippet: Comparison of the change in the cyclin A protein in human colonocytes and HCT116 colon cancer cells after incubation for 24 hours with deoxycholic acid based on a Western blot analysis. ERK, extracellular signal-regulated kinase.

    Article Snippet: The culture medium used in the cell culture was RPMI1640 (JEIL Biotech Services Incorp, Daegu, Korea), and fetal bovine serum (FBS, Hyclone Laboratories, Logaar, UT, USA), penicillin-streptomycin, and deoxycholate (Sigma, St Louis, MO, USA) were added reagents.

    Techniques: Incubation, Western Blot

    Isolated human colonic crypt (A) and colonocytes (B). Human normal colonocytes were incubated for 24 hours with various concentration of deoxycholic acid: 0 (C), 20 µM (D), and 250 µM (E).

    Journal: Journal of the Korean Society of Coloproctology

    Article Title: Effects of DCA on Cell Cycle Proteins in Colonocytes

    doi: 10.3393/jksc.2010.26.4.254

    Figure Lengend Snippet: Isolated human colonic crypt (A) and colonocytes (B). Human normal colonocytes were incubated for 24 hours with various concentration of deoxycholic acid: 0 (C), 20 µM (D), and 250 µM (E).

    Article Snippet: The culture medium used in the cell culture was RPMI1640 (JEIL Biotech Services Incorp, Daegu, Korea), and fetal bovine serum (FBS, Hyclone Laboratories, Logaar, UT, USA), penicillin-streptomycin, and deoxycholate (Sigma, St Louis, MO, USA) were added reagents.

    Techniques: Isolation, Incubation, Concentration Assay

    Change of cell-cycle-related proteins in human colonocytes after incubation for 24 hours with deoxycholic acid based on a Western blot analysis. Conc., concentration; ERK, extracellular signal-regulated kinase.

    Journal: Journal of the Korean Society of Coloproctology

    Article Title: Effects of DCA on Cell Cycle Proteins in Colonocytes

    doi: 10.3393/jksc.2010.26.4.254

    Figure Lengend Snippet: Change of cell-cycle-related proteins in human colonocytes after incubation for 24 hours with deoxycholic acid based on a Western blot analysis. Conc., concentration; ERK, extracellular signal-regulated kinase.

    Article Snippet: The culture medium used in the cell culture was RPMI1640 (JEIL Biotech Services Incorp, Daegu, Korea), and fetal bovine serum (FBS, Hyclone Laboratories, Logaar, UT, USA), penicillin-streptomycin, and deoxycholate (Sigma, St Louis, MO, USA) were added reagents.

    Techniques: Incubation, Western Blot, Concentration Assay

    Comparison of the change in cell-cycle-related proteins in the Caco-2 colorectal cancer cell-line after incubation for 72 hours with deoxycholic acid based on a Western blot analysis. Conc., concentration; ERK, extracellular signal-regulated kinase.

    Journal: Journal of the Korean Society of Coloproctology

    Article Title: Effects of DCA on Cell Cycle Proteins in Colonocytes

    doi: 10.3393/jksc.2010.26.4.254

    Figure Lengend Snippet: Comparison of the change in cell-cycle-related proteins in the Caco-2 colorectal cancer cell-line after incubation for 72 hours with deoxycholic acid based on a Western blot analysis. Conc., concentration; ERK, extracellular signal-regulated kinase.

    Article Snippet: The culture medium used in the cell culture was RPMI1640 (JEIL Biotech Services Incorp, Daegu, Korea), and fetal bovine serum (FBS, Hyclone Laboratories, Logaar, UT, USA), penicillin-streptomycin, and deoxycholate (Sigma, St Louis, MO, USA) were added reagents.

    Techniques: Incubation, Western Blot, Concentration Assay

    Comparison of the change in the cyclin E protein in human colonocytes and in HCT116 colon cancer cells after incubation for 24 hours with deoxycholic acid based on a Western blot analysis. ERK, extracellular signal-regulated kinase.

    Journal: Journal of the Korean Society of Coloproctology

    Article Title: Effects of DCA on Cell Cycle Proteins in Colonocytes

    doi: 10.3393/jksc.2010.26.4.254

    Figure Lengend Snippet: Comparison of the change in the cyclin E protein in human colonocytes and in HCT116 colon cancer cells after incubation for 24 hours with deoxycholic acid based on a Western blot analysis. ERK, extracellular signal-regulated kinase.

    Article Snippet: The culture medium used in the cell culture was RPMI1640 (JEIL Biotech Services Incorp, Daegu, Korea), and fetal bovine serum (FBS, Hyclone Laboratories, Logaar, UT, USA), penicillin-streptomycin, and deoxycholate (Sigma, St Louis, MO, USA) were added reagents.

    Techniques: Incubation, Western Blot

    Comparison of the change in the cyclin D1 protein in human colonocytes and HCT116 colon cancer cells after incubation for 24 hours with deoxycholic acid based on a Western blot analysis. ERK, extracellular signal-regulated kinase.

    Journal: Journal of the Korean Society of Coloproctology

    Article Title: Effects of DCA on Cell Cycle Proteins in Colonocytes

    doi: 10.3393/jksc.2010.26.4.254

    Figure Lengend Snippet: Comparison of the change in the cyclin D1 protein in human colonocytes and HCT116 colon cancer cells after incubation for 24 hours with deoxycholic acid based on a Western blot analysis. ERK, extracellular signal-regulated kinase.

    Article Snippet: The culture medium used in the cell culture was RPMI1640 (JEIL Biotech Services Incorp, Daegu, Korea), and fetal bovine serum (FBS, Hyclone Laboratories, Logaar, UT, USA), penicillin-streptomycin, and deoxycholate (Sigma, St Louis, MO, USA) were added reagents.

    Techniques: Incubation, Western Blot

    Comparison of the change of Cdk2 protein after incubation for 24 hours with deoxycholic acid in human colonocytes and HCT116 colon cancer cells by Western blot analysis. ERK, extracellular signal-regulated kinase.

    Journal: Journal of the Korean Society of Coloproctology

    Article Title: Effects of DCA on Cell Cycle Proteins in Colonocytes

    doi: 10.3393/jksc.2010.26.4.254

    Figure Lengend Snippet: Comparison of the change of Cdk2 protein after incubation for 24 hours with deoxycholic acid in human colonocytes and HCT116 colon cancer cells by Western blot analysis. ERK, extracellular signal-regulated kinase.

    Article Snippet: The culture medium used in the cell culture was RPMI1640 (JEIL Biotech Services Incorp, Daegu, Korea), and fetal bovine serum (FBS, Hyclone Laboratories, Logaar, UT, USA), penicillin-streptomycin, and deoxycholate (Sigma, St Louis, MO, USA) were added reagents.

    Techniques: Incubation, Western Blot

    Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium deoxycholate; C—chitosan-coated (0.5 mg/mL).

    Journal: Molecules

    Article Title: Chitosan-Coated Flexible Liposomes Magnify the Anticancer Activity and Bioavailability of Docetaxel: Impact on Composition

    doi: 10.3390/molecules24020250

    Figure Lengend Snippet: Zeta potentials of docetaxel (DXT)-loaded (5 mg/mL) liposomes and chitosomes. The data are expressed as means ± SD, n = 3. CL—conventional liposome; FL—flexible liposome; T—Tween-80; DP—dicetyl phosphate; NDC—sodium deoxycholate; C—chitosan-coated (0.5 mg/mL).

    Article Snippet: Sodium deoxycholate, methanol, and acetonitrile (HPLC grade) were purchased from Fisher Scientific Co., (Hampton, NH, USA).

    Techniques:

    Weighted chemical shift difference (Δδ HN ) of the 1 H and 15 N resonances of SipD 39–343 when titrated with (A) deoxycholate, (B) taurodeoxycholate and (C) chenodeoxycholate (Δδ HN = [½ (δ H 2 + 1/25δ

    Journal: Biochemistry

    Article Title: NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts

    doi: 10.1021/bi100335u

    Figure Lengend Snippet: Weighted chemical shift difference (Δδ HN ) of the 1 H and 15 N resonances of SipD 39–343 when titrated with (A) deoxycholate, (B) taurodeoxycholate and (C) chenodeoxycholate (Δδ HN = [½ (δ H 2 + 1/25δ

    Article Snippet: For NMR chemical shift mapping, 2D 1 H-15 N TROSY-HSQC ( ) spectra were acquired using 15 N-labeled SipD39–343 that was titrated with varying amounts of deoxycholate (Amresco), chenodeoxycholate (Sigma), taurodeoxycholate (Sigma) or cholate hydrate (Sigma).

    Techniques:

    (A) Overlay of four 2D 1 H- 15 N TROSY spectra of [ 2 H, 15 N, 13 C]-labeled SipD 39–343 with increasing amounts of deoxycholate. Some of the assignments are indicated as well as the noise peaks (*). (B) Expanded regions of the 2D 1 H- 15 N TROSY spectra for

    Journal: Biochemistry

    Article Title: NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts

    doi: 10.1021/bi100335u

    Figure Lengend Snippet: (A) Overlay of four 2D 1 H- 15 N TROSY spectra of [ 2 H, 15 N, 13 C]-labeled SipD 39–343 with increasing amounts of deoxycholate. Some of the assignments are indicated as well as the noise peaks (*). (B) Expanded regions of the 2D 1 H- 15 N TROSY spectra for

    Article Snippet: For NMR chemical shift mapping, 2D 1 H-15 N TROSY-HSQC ( ) spectra were acquired using 15 N-labeled SipD39–343 that was titrated with varying amounts of deoxycholate (Amresco), chenodeoxycholate (Sigma), taurodeoxycholate (Sigma) or cholate hydrate (Sigma).

    Techniques: Labeling

    Ribbon representation of the crystal structure of SipD 39–343 (to be reported elsewhere). The side chains of residues that showed significant chemical shift perturbation (Δδ HN > 0.03) with deoxycholate were colored red (legend:

    Journal: Biochemistry

    Article Title: NMR characterization of the interaction of the Salmonella type III secretion system protein SipD and bile salts

    doi: 10.1021/bi100335u

    Figure Lengend Snippet: Ribbon representation of the crystal structure of SipD 39–343 (to be reported elsewhere). The side chains of residues that showed significant chemical shift perturbation (Δδ HN > 0.03) with deoxycholate were colored red (legend:

    Article Snippet: For NMR chemical shift mapping, 2D 1 H-15 N TROSY-HSQC ( ) spectra were acquired using 15 N-labeled SipD39–343 that was titrated with varying amounts of deoxycholate (Amresco), chenodeoxycholate (Sigma), taurodeoxycholate (Sigma) or cholate hydrate (Sigma).

    Techniques:

    MAGE-A11 interacts with p107 and Rb but not p130. pCMV-FLAG (−), pCMV-FLAG-MAGE, or FLAG-MAGE-(112–429) (5 μg) was expressed in COS1 cells with 5 μg of CMV-p107 ( A ), pCMV-hRb ( B ), or pcDNA3-p130 ( C ). Cells were incubated for 24 h in serum-free medium containing 0.1 μg/ml EGF and harvested in immunoprecipitation lysis buffer without deoxycholate or glycerol. Cell extracts (50 μg of protein/lane, right ) and immunoprecipitates ( IP , left ) were probed using p107, Rb, p130, and MAGE-A11 antibodies. Arrows , p107, Rb, p130, FLAG-MAGE, and IgG.

    Journal: The Journal of Biological Chemistry

    Article Title: Proto-oncogene Activity of Melanoma Antigen-A11 (MAGE-A11) Regulates Retinoblastoma-related p107 and E2F1 Proteins *

    doi: 10.1074/jbc.M113.468579

    Figure Lengend Snippet: MAGE-A11 interacts with p107 and Rb but not p130. pCMV-FLAG (−), pCMV-FLAG-MAGE, or FLAG-MAGE-(112–429) (5 μg) was expressed in COS1 cells with 5 μg of CMV-p107 ( A ), pCMV-hRb ( B ), or pcDNA3-p130 ( C ). Cells were incubated for 24 h in serum-free medium containing 0.1 μg/ml EGF and harvested in immunoprecipitation lysis buffer without deoxycholate or glycerol. Cell extracts (50 μg of protein/lane, right ) and immunoprecipitates ( IP , left ) were probed using p107, Rb, p130, and MAGE-A11 antibodies. Arrows , p107, Rb, p130, FLAG-MAGE, and IgG.

    Article Snippet: Immunoblots of extracts from COS1 cells (2 × 106 cells/10-cm dish, 7 × 105 cells/6-cm dish) transfected using DEAE-dextran were prepared in immunoblot lysis buffer containing 1% Triton X-100, 0.1% SDS, 1% sodium deoxycholate, 0.15 m NaCl, 2 m m EDTA, 50 m m NaF, 2 m m sodium vanadate, 50 m m Tris-HCl (pH 7.5), 1 m m phenylmethylsulfonyl fluoride, 1 m m dithiothreitol, and protease inhibitors (Roche Applied Science).

    Techniques: Incubation, Immunoprecipitation, Lysis

    Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium deoxycholate complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.

    Journal: International Journal of Molecular Sciences

    Article Title: Multifunctional Composite Microcapsules for Oral Delivery of Insulin

    doi: 10.3390/ijms18010054

    Figure Lengend Snippet: Diagram of the preparation of multifunctional composite microcapsules. Ins-SD complex, insulin-sodium deoxycholate complex; PVA, polyvinyl alcohol; PLGA, poly(lactide- co -glycolide); NPs, nanoparticles, HP55, hydroxypropyl methyl cellulose phthalate.

    Article Snippet: Sodium deoxycholate and poly(lactic-co -glycolic acid) (PLGA 75/25, molecular weight of 20 kDa) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).

    Techniques:

    Bile acid metabolism in animals treated with vehicle or TCDF (24 μg/kg). Abbreviations: CA, cholic acid; CDCA, chenodeoxycholic acid; DCA, deoxycholic acid; G, glycine-conjugated; LCA, lithocholic acid; MCA, muricholic acid; NS, not significant; T, taurine-conjugated species; UDCA, ursodeoxycholic acid. Quantification of specific bile acids levels in intestinal tissue ( A,B ) and feces ( C,D ) throughout the enterohepatic circulation of Ahr +/+ mice. ( E ) Quantification of total bile acids in liver, intestine, cecum, and feces of Ahr +/+ mice; the bile acid profile in the small intestine shows the data from jejunum segment. qPCR analysis of Fgf15 , Fxr, and Shp mRNAs in the ileum ( F ) and Fxr and Shp mRNA expression in the liver ( G ) of Ahr +/+ and Ahr –/– mice. ( H ) qPCR analysis of Cyp7a1 , Cyp8b1 , Cyp27a1 , Akr1d1, and Cyp7b1 mRNAs in the liver of Ahr +/+ mice. ( I – K ) mRNA encoding bile acid transporters involved in taurine biosynthesis and bile acid conjugation in the ileum ( I ), and mRNA encoding bile acid transporters in the distal liver ( J ) and ileum ( K ) of vehicle- and TCDF-treated Ahr +/+ mice. Data are presented as mean ± SD; n = 6/group. See also Supplemental Material, Tables S1 and S2. * p

    Journal: Environmental Health Perspectives

    Article Title: Persistent Organic Pollutants Modify Gut Microbiota–Host Metabolic Homeostasis in Mice Through Aryl Hydrocarbon Receptor Activation

    doi: 10.1289/ehp.1409055

    Figure Lengend Snippet: Bile acid metabolism in animals treated with vehicle or TCDF (24 μg/kg). Abbreviations: CA, cholic acid; CDCA, chenodeoxycholic acid; DCA, deoxycholic acid; G, glycine-conjugated; LCA, lithocholic acid; MCA, muricholic acid; NS, not significant; T, taurine-conjugated species; UDCA, ursodeoxycholic acid. Quantification of specific bile acids levels in intestinal tissue ( A,B ) and feces ( C,D ) throughout the enterohepatic circulation of Ahr +/+ mice. ( E ) Quantification of total bile acids in liver, intestine, cecum, and feces of Ahr +/+ mice; the bile acid profile in the small intestine shows the data from jejunum segment. qPCR analysis of Fgf15 , Fxr, and Shp mRNAs in the ileum ( F ) and Fxr and Shp mRNA expression in the liver ( G ) of Ahr +/+ and Ahr –/– mice. ( H ) qPCR analysis of Cyp7a1 , Cyp8b1 , Cyp27a1 , Akr1d1, and Cyp7b1 mRNAs in the liver of Ahr +/+ mice. ( I – K ) mRNA encoding bile acid transporters involved in taurine biosynthesis and bile acid conjugation in the ileum ( I ), and mRNA encoding bile acid transporters in the distal liver ( J ) and ileum ( K ) of vehicle- and TCDF-treated Ahr +/+ mice. Data are presented as mean ± SD; n = 6/group. See also Supplemental Material, Tables S1 and S2. * p

    Article Snippet: External bile acid standards, including cholic acid (CA), lithocholic acid (LCA), chenodeoxycholic acid (CDCA), deoxycholic acid (DCA), ursodeoxycholic acid (UDCA), α-muricholic acid (αMCA), β-muricholic acid (βMCA), ω-muricholic acid (ωMCA), glycocholic acid (GCA), glycochenodeoxycholic acid (GCDCA), taurocholic acid (TCA), taurodeoxycholic acid (TDCA), tauro chenodeoxycholic acid (TCDCA), taurolithocholic acid (TLCA), tauro-α-muricholic acid (TαMCA), and tauro-β-muricholic acid (TβMCA), were purchased from Sigma-Aldrich.

    Techniques: Mouse Assay, Real-time Polymerase Chain Reaction, Expressing, Conjugation Assay

    The effect of RA incorporated DexDA-2 nanoparticles on the invasion ( A ) and MMP-2 expression ( B ) of CT26 tumor cells. Notes: Serum free media was treated for control. CT26 cells were exposed to empty nanoparticles, All-trans retinoic acid (RA), and RA-incorporated nanoparticles of DexDA-2 (NP) for 24h. After that, cells were trypsinized, washed with PBS, and seeded onto upper chamber (primarily coated with Matrigel) to invade. Abbreviations: RA, all- trans retinoic acid; DexDA, deoxycholic acid-conjugated dextran; EN10, empty nanoparticles of DexDA-2, 10 μg/mL; EN100, empty nanoparticles of DexDA-2, 100 μg/mL; RA1, RA 1 μg/mL; RA10, RA 10 μg/mL; RA20, RA 20 μg/mL; RA50, RA 50 μg/mL; NP1, RA-incorporated nanoparticles of DexDA-2 (RA dose 1 μg/mL); NP10, RA-incorporated nanoparticles of DexDA-2 (RA dose 10 μg/mL); NP20, RA-incorporated nanoparticles of DexDA-2 (RA dose 20 μg/mL); NP50, RA-incorporated nanoparticles of DexDA-2 (RA dose 50 μg/mL); MMP-2, matrix metalloproteinases-2.

    Journal: International Journal of Nanomedicine

    Article Title: All-trans retinoic acid-incorporated nanoparticles of deoxycholic acid-conjugated dextran for treatment of CT26 colorectal carcinoma cells

    doi: 10.2147/IJN.S40580

    Figure Lengend Snippet: The effect of RA incorporated DexDA-2 nanoparticles on the invasion ( A ) and MMP-2 expression ( B ) of CT26 tumor cells. Notes: Serum free media was treated for control. CT26 cells were exposed to empty nanoparticles, All-trans retinoic acid (RA), and RA-incorporated nanoparticles of DexDA-2 (NP) for 24h. After that, cells were trypsinized, washed with PBS, and seeded onto upper chamber (primarily coated with Matrigel) to invade. Abbreviations: RA, all- trans retinoic acid; DexDA, deoxycholic acid-conjugated dextran; EN10, empty nanoparticles of DexDA-2, 10 μg/mL; EN100, empty nanoparticles of DexDA-2, 100 μg/mL; RA1, RA 1 μg/mL; RA10, RA 10 μg/mL; RA20, RA 20 μg/mL; RA50, RA 50 μg/mL; NP1, RA-incorporated nanoparticles of DexDA-2 (RA dose 1 μg/mL); NP10, RA-incorporated nanoparticles of DexDA-2 (RA dose 10 μg/mL); NP20, RA-incorporated nanoparticles of DexDA-2 (RA dose 20 μg/mL); NP50, RA-incorporated nanoparticles of DexDA-2 (RA dose 50 μg/mL); MMP-2, matrix metalloproteinases-2.

    Article Snippet: Materials Dextran from Leuconostoc mesenteroides (average molecular weights 38,900), RA, deoxycholic acid (DA), thiazolyl blue tetrazolium bromide (MTT), N,N′-Dicyclohexylcarbodiimide (DCC), and 4-(N ,N -dimethylamino)pyridine (DMAP) were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Techniques: Expressing

    Intrinsic cytotoxicity of empty nanoparticles of deoxycholic acid-conjugated dextran (DexDA) against RAW 264.7 mouse macrophage cells. Notes: For intrinsic cytotoxicity of empty nanoparticles of DexDA conjugates, 3 × 10 4 RAW264.7 mouse macrophage cells were seeded in 96-well plates and exposed to empty DexDA nanoparticles. All treatment was incubated for 2 days. The viability of cells was evaluated by thiazolyl blue tetrazolium bromide (MTT) assay, as described in the Materials and methods section.

    Journal: International Journal of Nanomedicine

    Article Title: All-trans retinoic acid-incorporated nanoparticles of deoxycholic acid-conjugated dextran for treatment of CT26 colorectal carcinoma cells

    doi: 10.2147/IJN.S40580

    Figure Lengend Snippet: Intrinsic cytotoxicity of empty nanoparticles of deoxycholic acid-conjugated dextran (DexDA) against RAW 264.7 mouse macrophage cells. Notes: For intrinsic cytotoxicity of empty nanoparticles of DexDA conjugates, 3 × 10 4 RAW264.7 mouse macrophage cells were seeded in 96-well plates and exposed to empty DexDA nanoparticles. All treatment was incubated for 2 days. The viability of cells was evaluated by thiazolyl blue tetrazolium bromide (MTT) assay, as described in the Materials and methods section.

    Article Snippet: Materials Dextran from Leuconostoc mesenteroides (average molecular weights 38,900), RA, deoxycholic acid (DA), thiazolyl blue tetrazolium bromide (MTT), N,N′-Dicyclohexylcarbodiimide (DCC), and 4-(N ,N -dimethylamino)pyridine (DMAP) were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Techniques: Incubation, MTT Assay

    The effect of RA-incorporated DexDA-2 nanoparticles on the proliferation of CT26 tumor cells. Abbreviations: RA, all- trans retinoic acid; DexDA, deoxycholic acid-conjugated dextran; NP, RA-incorporated nanoparticles of DexDA-2; empty NP, empty nanoparticles of DexDA-2.

    Journal: International Journal of Nanomedicine

    Article Title: All-trans retinoic acid-incorporated nanoparticles of deoxycholic acid-conjugated dextran for treatment of CT26 colorectal carcinoma cells

    doi: 10.2147/IJN.S40580

    Figure Lengend Snippet: The effect of RA-incorporated DexDA-2 nanoparticles on the proliferation of CT26 tumor cells. Abbreviations: RA, all- trans retinoic acid; DexDA, deoxycholic acid-conjugated dextran; NP, RA-incorporated nanoparticles of DexDA-2; empty NP, empty nanoparticles of DexDA-2.

    Article Snippet: Materials Dextran from Leuconostoc mesenteroides (average molecular weights 38,900), RA, deoxycholic acid (DA), thiazolyl blue tetrazolium bromide (MTT), N,N′-Dicyclohexylcarbodiimide (DCC), and 4-(N ,N -dimethylamino)pyridine (DMAP) were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Techniques:

    Particle-size changes according to the drug content in the nanoparticles ( A ) and substitution degree (DS) of deoxycholic acid in the deoxycholic acid-conjugated dextran (DexDA) conjugates ( B ).

    Journal: International Journal of Nanomedicine

    Article Title: All-trans retinoic acid-incorporated nanoparticles of deoxycholic acid-conjugated dextran for treatment of CT26 colorectal carcinoma cells

    doi: 10.2147/IJN.S40580

    Figure Lengend Snippet: Particle-size changes according to the drug content in the nanoparticles ( A ) and substitution degree (DS) of deoxycholic acid in the deoxycholic acid-conjugated dextran (DexDA) conjugates ( B ).

    Article Snippet: Materials Dextran from Leuconostoc mesenteroides (average molecular weights 38,900), RA, deoxycholic acid (DA), thiazolyl blue tetrazolium bromide (MTT), N,N′-Dicyclohexylcarbodiimide (DCC), and 4-(N ,N -dimethylamino)pyridine (DMAP) were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Techniques:

    ( A and B ) RA release from DexDA nanoparticles. ( A ) The effect of drug content in the nanoparticles; ( B ) effect of substitution degree of deoxycholic acid in the conjugates. Notes: * P

    Journal: International Journal of Nanomedicine

    Article Title: All-trans retinoic acid-incorporated nanoparticles of deoxycholic acid-conjugated dextran for treatment of CT26 colorectal carcinoma cells

    doi: 10.2147/IJN.S40580

    Figure Lengend Snippet: ( A and B ) RA release from DexDA nanoparticles. ( A ) The effect of drug content in the nanoparticles; ( B ) effect of substitution degree of deoxycholic acid in the conjugates. Notes: * P

    Article Snippet: Materials Dextran from Leuconostoc mesenteroides (average molecular weights 38,900), RA, deoxycholic acid (DA), thiazolyl blue tetrazolium bromide (MTT), N,N′-Dicyclohexylcarbodiimide (DCC), and 4-(N ,N -dimethylamino)pyridine (DMAP) were purchased from Sigma-Aldrich (St Louis, MO, USA).

    Techniques:

    Representative longitudinal cross-sectional images of proximal rat tibiae stained with hematoxylin and eosin (H E) that were either sham-operated (SHAM) or ovariectomized (OVX) and treated with various modes. Notes: Cross-sectional images were either SHAM ( A ) or OVX ( B ). The treatment modes were once-daily subcutaneous injection of 10 μg kg −1 rhPTH (1-34) (OVX-PTH-SC) ( C ) or once-daily oral administration of 50 μg kg −1 rhPTH (1-34) (OVX-PTH-ORAL) ( D ), rhPTH (1-34)/Lys-DOCA complex as 50 μg kg −1 of rhPTH (1-34) (OVX-PTH/LysDOCA-ORAL) ( E ), or enteric-microparticles containing rhPTH (1-34)/Lys-DOCA nanocomplex as 50 μg kg −1 of rhPTH (1-34) (OVX-PTH/LysDOCA-MP-ORAL) ( F ) for 12 weeks (n=10 for each group). Scale bar =200 μm. Abbreviations: PTH, parathyroid hormone; recombinant human PTH; LysDOCA, lysine-linked deoxycholic acid.

    Journal: International Journal of Nanomedicine

    Article Title: Preparation and in vivo evaluation of an orally available enteric-microencapsulated parathyroid hormone (1-34)-deoxycholic acid nanocomplex

    doi: 10.2147/IJN.S110573

    Figure Lengend Snippet: Representative longitudinal cross-sectional images of proximal rat tibiae stained with hematoxylin and eosin (H E) that were either sham-operated (SHAM) or ovariectomized (OVX) and treated with various modes. Notes: Cross-sectional images were either SHAM ( A ) or OVX ( B ). The treatment modes were once-daily subcutaneous injection of 10 μg kg −1 rhPTH (1-34) (OVX-PTH-SC) ( C ) or once-daily oral administration of 50 μg kg −1 rhPTH (1-34) (OVX-PTH-ORAL) ( D ), rhPTH (1-34)/Lys-DOCA complex as 50 μg kg −1 of rhPTH (1-34) (OVX-PTH/LysDOCA-ORAL) ( E ), or enteric-microparticles containing rhPTH (1-34)/Lys-DOCA nanocomplex as 50 μg kg −1 of rhPTH (1-34) (OVX-PTH/LysDOCA-MP-ORAL) ( F ) for 12 weeks (n=10 for each group). Scale bar =200 μm. Abbreviations: PTH, parathyroid hormone; recombinant human PTH; LysDOCA, lysine-linked deoxycholic acid.

    Article Snippet: N -Methylmorpholine, deoxycholic acid (DOCA), ethyl chloroformate, N ε -Boc-l -lysine methyl ester hydrochloride (H-Lys(Boc)-OMe·HCl), and 2,2,2-trifluoroethanol (TFE) were from Sigma-Aldrich Co. (St Louis, MO, USA).

    Techniques: Staining, Injection, Recombinant

    Representative 2D and 3D images of the distal rat tibiae that were either sham-operated (SHAM) or ovariectomized (OVX) and treated with various modes. Notes: Distal rat tibiae were either SHAM ( A ) or OVX ( B ). The treatment modes were once-daily subcutaneous injection of 10 μg kg −1 rhPTH (1-34) (OVX-PTH-SC) ( C ) or once-daily oral administration of 50 μg kg −1 rhPTH (1-34) (OVX-PTH-ORAL) ( D ), rhPTH (1-34)/Lys-DOCA complex as 50 μg kg −1 of rhPTH (1-34) (OVX-PTH/LysDOCA-ORAL) ( E ), or enteric-microparticles containing rhPTH (1-34)/Lys-DOCA nanocomplex as 50 μg kg −1 of rhPTH (1-34) (OVX-PTH/LysDOCA-MP-ORAL) ( F ) for 12 weeks (n=10 for each group). Abbreviations: PTH, parathyroid hormone; recombinant human PTH; LysDOCA, lysine-linked deoxycholic acid.

    Journal: International Journal of Nanomedicine

    Article Title: Preparation and in vivo evaluation of an orally available enteric-microencapsulated parathyroid hormone (1-34)-deoxycholic acid nanocomplex

    doi: 10.2147/IJN.S110573

    Figure Lengend Snippet: Representative 2D and 3D images of the distal rat tibiae that were either sham-operated (SHAM) or ovariectomized (OVX) and treated with various modes. Notes: Distal rat tibiae were either SHAM ( A ) or OVX ( B ). The treatment modes were once-daily subcutaneous injection of 10 μg kg −1 rhPTH (1-34) (OVX-PTH-SC) ( C ) or once-daily oral administration of 50 μg kg −1 rhPTH (1-34) (OVX-PTH-ORAL) ( D ), rhPTH (1-34)/Lys-DOCA complex as 50 μg kg −1 of rhPTH (1-34) (OVX-PTH/LysDOCA-ORAL) ( E ), or enteric-microparticles containing rhPTH (1-34)/Lys-DOCA nanocomplex as 50 μg kg −1 of rhPTH (1-34) (OVX-PTH/LysDOCA-MP-ORAL) ( F ) for 12 weeks (n=10 for each group). Abbreviations: PTH, parathyroid hormone; recombinant human PTH; LysDOCA, lysine-linked deoxycholic acid.

    Article Snippet: N -Methylmorpholine, deoxycholic acid (DOCA), ethyl chloroformate, N ε -Boc-l -lysine methyl ester hydrochloride (H-Lys(Boc)-OMe·HCl), and 2,2,2-trifluoroethanol (TFE) were from Sigma-Aldrich Co. (St Louis, MO, USA).

    Techniques: Injection, Recombinant

    Bile salt hydrolase (BSH) enzyme activity assay. Taurodeoxycholic acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p

    Journal: Methods (San Diego, Calif.)

    Article Title: Functional Microbiomics: Evaluation of Gut Microbiota-Bile Acid Metabolism Interactions in Health and Disease

    doi: 10.1016/j.ymeth.2018.04.028

    Figure Lengend Snippet: Bile salt hydrolase (BSH) enzyme activity assay. Taurodeoxycholic acid was used as the substrate for the enzyme assay, and results are therefore expressed as rate of deoxycholic acid formation (* p

    Article Snippet: Faecal protein incubated with phosphate-buffered saline served as a negative control, and faecal protein incubated with varying concentrations of deoxycholic acid (Merck) was used to establish a standard curve to quantify precipitate formation.

    Techniques: Enzyme Activity Assay, Enzymatic Assay