Journal: Frontiers in Cardiovascular Medicine

Article Title: Hematopoietic Cell-Specific SLC37A2 Deficiency Accelerates Atherosclerosis in LDL Receptor-Deficient Mice

doi: 10.3389/fcvm.2021.777098

Figure Lengend Snippet: SLC37A2 deficiency promotes oxidized LDL (OxLDL)-induced macrophage inflammation in vitro . (A) SLC37A2 protein expression analyzed by Western blotting in WT and Slc37a2 −/− bone marrow-derived macrophages (BMDMs) treated with 50 μg/ml oxLDL for 0-24 h. (B) Quantification of cellar total cholesterol (TC), free cholesterol (FC), and cholesterol ester (CE) in WT and Slc37a2 −/− BMDMs after treated with or without 25 or 50 μg/ml oxLDL for 24 h. (C) Relative transcript level of cytokines in WT and Slc37a2 −/− BMDMs stimulated with 50 μg/ml oxLDL for 0-24 h, measured by qPCR. Data are representative of two independent experiments with three samples per group (mean ± SEM). * p < 0.05; unpaired, two-tailed Student's t -test.

Article Snippet: To induce foam cell formation, BMDMs were treated with 25 or 50 μg/ml oxLDL (Athens Research & Technology) for 0-24 h. In some experiments, BMDMs were pretreated for 30 min with hexokinase inhibitor 2-deoxy-D-glucose (2-DG; 10 mM, Sigma-Aldrich), actin polymerization inhibitor cytochalasin D (10 μM, Cayman), or fatty acid β-oxidation inhibitor etomoxir (50 μM, Cayman) and subsequently treated with apoptotic cells for an additional 4 h in the presence of each inhibitor.

Techniques: In Vitro, Expressing, Western Blot, Derivative Assay, Two Tailed Test

Journal: International journal of medical sciences

Article Title: P66Shc is increased in peripheral blood mononuclear cells of the patients with obstructive sleep apnea.

doi: 10.7150/ijms.80343

Figure Lengend Snippet: Figure 2. P66Shc mRNA levels (n=73) were positively correlated with (A) plasma 3-NT, (B) oxLDL, and (C) AOPP levels by Spearman’s rank correlation coefficient.

Article Snippet: Enzyme-linked Immunosorbent Assay The level of plasma 3-NT (Elabscience, Catalog#E-EL-0040c, China), oxLDL (Elabscience, Catalog#E-EL-H6021, China) and AOPP (Abbkine, Catalog#KTB1060, China) were detected with the enzyme-linked immunosorbent assay (ELISA) commercial kits according to the recommendations of the manufacturers.

Techniques: Clinical Proteomics

Journal: Drug Design, Development and Therapy

Article Title: Baolier Capsule’s Secret Weapon: Piperine Boosts Cholesterol Excretion to Combat Atherosclerosis

doi: 10.2147/DDDT.S499598

Figure Lengend Snippet: Key Resources Table

Article Snippet: LDL-C assay kit , Elabscience , E-BC-K205-M.

Techniques: Control, shRNA, Sequencing, Staining, Fluorescence, Microscopy, AST Assay, Phospholipid Assay, Mass Spectrometry