Journal: BMC Biotechnology

Article Title: In utero recombinant adeno-associated virus gene transfer in mice, rats, and primates

doi: 10.1186/1472-6750-3-16

Figure Lengend Snippet: rAAV2 mediated gene transfer in the mouse fetus. Fetuses at 15–16 day gestation were infected with rAAV2CMVgfp via intra-amniotic transfer. At 72 hours post-infection whole fetuses were fixed and section for deconvoluting microscopic analysis. Sections were stained with the nuclear stain DAPI (blue) and phalloidin (red) staining of actin. A-lung; B-intestines; and C-kidney. Original magnification 630X

Article Snippet: Fluorescent analyses of samples were performed in a Leitz deconvoluting microscope.

Techniques: Infection, Staining

Journal: BMC Biotechnology

Article Title: In utero recombinant adeno-associated virus gene transfer in mice, rats, and primates

doi: 10.1186/1472-6750-3-16

Figure Lengend Snippet: Persistence and expression of transgenes following in utero rAAV2 gene therapy. In utero gene therapy withrAAV2CMVgfp was performed in NHP and the tissues analyzed for GFP expression at 15 months of age. All sections were stained with the nuclear-specific DAPI (blue color) and visualized on a deconvoluting microscope. A – Lung; B – Intestines; and C – Kidney. Original magnification 630X

Article Snippet: Fluorescent analyses of samples were performed in a Leitz deconvoluting microscope.

Techniques: Expressing, In Utero, Staining, Microscopy

Journal: BMC Biotechnology

Article Title: In utero recombinant adeno-associated virus gene transfer in mice, rats, and primates

doi: 10.1186/1472-6750-3-16

Figure Lengend Snippet: High resolution, three color analysis of GFP protein in NHP intestines. Frozen sections from a 15 month old NHP were stained with the nuclear stain DAPI (blue color) and the actin-specific phalloidin conjugated to fluorescent marker (red color). Three dimensional reconstructions were performed on an automated, deconvoluting microscope. Original magnification 630X

Article Snippet: Fluorescent analyses of samples were performed in a Leitz deconvoluting microscope.

Techniques: Staining, Marker, Microscopy

Journal: mSphere

Article Title: Unique dermal bacterial signature differentiates atopic dermatitis skin from healthy

doi: 10.1128/msphere.00156-25

Figure Lengend Snippet: Schematic of participants, locations, samples, and analysis and metadata of participants in dot plots. Samples (a) were collected at three different habitats—dry (d), moist (m), and sebaceous (s) from the cheek, elbow pit, and back of hand, respectively. Fifteen consecutive tape strips (b) were collected for confocal laser scanning microscopy (CLSM) (tapes 1, 6, and 15), cultivation, and MALDI-TOF (pools 2–5, 7–10, and 11–14). Additionally (c), four biopsies, two from each elbow pit and back of hand, were collected for CLSM and metagenomics, respectively. The latter were divided into epidermal and dermal compartments before DNA extraction. (d) The distribution of age and sex is shown for the two groups of participants, atopic dermatitis (AD) and healthy (HC). Each dot represents one participant. The healthy participants were sex- and age-matched to the included AD patients. The average age of the participants was 34 years. (e) The total severity score (TSS) of eczema based on lichenification, excoriation, erythema, crusting/oozing, dryness/scaling, and edema (score of 0–3) is shown. Each dot represents the total TSS of the sampled area. Eczema categories: non-lesional = 0–1; mild = 2–5; moderate = 6–9; and severe = 10–18. TSS is shown with dotted lines.

Article Snippet: Representative images were taken using the software ZEN black (Zeiss) and deconvoluted using the interactive microscopy image analysis (Imaris) version 9 (Bitplane, Zurich, Switzerland) ( ).

Techniques: Confocal Laser Scanning Microscopy, DNA Extraction