Journal: International Journal of Molecular Sciences

Article Title: Positive Allosteric Modulator of SERCA Pump NDC-1173 Exerts Beneficial Effects in Mouse Model of Alzheimer’s Disease

doi: 10.3390/ijms241311057

Figure Lengend Snippet: ER-stress-marker genes and primers. C/EBP homologous protein (CHOP/Ddit3), binding immunoglobulin protein (BiP/Grp78), activating transcription factor 6 (ATF6), activating transcription factor 4 (ATF4), and X-box binding protein 1 spliced variant (XBP1spl) with associated primer sequences used for qPCR experiments.

Article Snippet: CHOP (Ddit3) , FP 5′-GGAGGTCCTGTCCTCAGATGAA-3′ RP 5′-GCTCCTCTGTCAGCCAAGCTAG-3′ , OriGene.

Techniques: Binding Assay, Variant Assay

Journal: International Journal of Molecular Sciences

Article Title: Positive Allosteric Modulator of SERCA Pump NDC-1173 Exerts Beneficial Effects in Mouse Model of Alzheimer’s Disease

doi: 10.3390/ijms241311057

Figure Lengend Snippet: The effects of NDC-1173 on expression of ER-stress-related markers in APP/PS1 mice. ( A ) C/EBP homologous protein (CHOP/Ddit3), ( B ) binding immunoglobulin protein (BiP/Grp78), ( C ) activating transcription factor 6 (ATF6), ( D ) activating transcription factor 4 (ATF4), and ( E ) X-box binding protein 1 spliced variant (XBP1spl) were detected by real-time quantitative PCR and normalized to Cyclophilin. mRNA expression of ER-stress-related markers were reduced in APP/PS1 mice treated with NDN compound 1173 compared to the untreated vehicle group (VEH). Data expressed as mean ± SD ( n = 9–11). Statistical significance determined by two-tailed unpaired t -tests. *** p < 0.001 compared to untreated APP/PS1 mice.

Article Snippet: CHOP (Ddit3) , FP 5′-GGAGGTCCTGTCCTCAGATGAA-3′ RP 5′-GCTCCTCTGTCAGCCAAGCTAG-3′ , OriGene.

Techniques: Expressing, Binding Assay, Variant Assay, Real-time Polymerase Chain Reaction, Two Tailed Test

Journal: International Journal of Molecular Medicine

Article Title: Isorhamnetin protects against bleomycin-induced pulmonary fibrosis by inhibiting endoplasmic reticulum stress and epithelial-mesenchymal transition

doi: 10.3892/ijmm.2018.3965

Figure Lengend Snippet: Effects of Isor on BLM-induced pulmonary endoplasmic reticulum stress markers. (A) The protein expression levels of CHOP and GRP78 were assessed by western blot analysis, and the densitometry values were normalized to β-actin. (B) The mRNA expression levels of CHOP and GRP78 were determined by reverse transcription-quantitative polymerase chain reaction. (C) Immunohistochemistry staining of CHOP in lung tissue sections (magnification, ×200 and ×400). Data are presented as the mean ± standard deviation (n=3). * P<0.05, ** P<0.01 and *** P<0.001, with comparisons indicated by lines. Isor, isorhamnetin; BLM, bleomycin; CHOP, DNA damage-inducible transcript 3; GRP78, 78 kDa glucose-regulating protein.

Article Snippet: Antibodies targeting vimentin (dilution, 1:1,200; YT4880), phosphorylated (p)-PERK (dilution, 1:2,000; cat. no. YP1055), DNA damage-inducible transcript 3 (DDIT3; also known as CHOP; dilution, 1:1,200; cat. no. YT0911), eukaryotic translation initiation factor 2 subunit α (eIF2α; dilution, 1:1,200; cat. no. YT1507) and p-eIF2α (dilution, 1:1,000; cat. no. YP0093) were obtained from ImmunoWay Biotechnology, Plano, TX, USA.

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Immunohistochemistry, Staining, Standard Deviation

Journal: International Journal of Molecular Medicine

Article Title: Isorhamnetin protects against bleomycin-induced pulmonary fibrosis by inhibiting endoplasmic reticulum stress and epithelial-mesenchymal transition

doi: 10.3892/ijmm.2018.3965

Figure Lengend Snippet: Effects of Isor on the expression of endoplasmic reticulum stress markers in TGFβ1-induced A549 cells and HBECs. The protein expression levels of CHOP and GRP78 were assessed by western blot analysis in A549 cells and in HBECs, and the densitometry values were normalized to β-actin. Data are presented as the mean ± standard deviation (n=3). ** P<0.01 and *** P<0.001, with comparisons indicated by lines. Isor, isorhamnetin; TGFβ1, transforming growth factor β1; HBECs, human bronchial epithelial cells; CHOP, DNA damage-inducible transcript 3; GRP78, 78 kDa glucose-regulating protein.

Article Snippet: Antibodies targeting vimentin (dilution, 1:1,200; YT4880), phosphorylated (p)-PERK (dilution, 1:2,000; cat. no. YP1055), DNA damage-inducible transcript 3 (DDIT3; also known as CHOP; dilution, 1:1,200; cat. no. YT0911), eukaryotic translation initiation factor 2 subunit α (eIF2α; dilution, 1:1,200; cat. no. YT1507) and p-eIF2α (dilution, 1:1,000; cat. no. YP0093) were obtained from ImmunoWay Biotechnology, Plano, TX, USA.

Techniques: Expressing, Western Blot, Standard Deviation