dde Search Results


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  • 94
    Millipore dde
    <t>DDE</t> activates cell signaling proteins MAPK and VASP in prostate cancer cells. LNCaP ( A ) and LAPC4 ( B ) cells were cultured in the absence of steroid for 24 h, and then either vehicle control (0.1% ethanol), 10 −10 mol/L <t>DHT,</t> or 10 −11 mol/L DDE was added. Following 40 or 120 min, cell lysates were collected and subjected to SDS-PAGE followed by immunoblotting for total ERK1/2, p-ERK1/2, or lamin B (loading control). C. LNCaP and LAPC4 cells were cultured in the absence of steroid for 24 h before treating with either 0.1% ethanol, 10 −10 mol/L DHT, or 10 −11 mol/L DDE. C. Following 10-min incubation, cells were harvested and the cell lysates were subjected to SDS-PAGE for expression analysis of phosphorylated VASP ( p-VASP ) or lamin B (loading control). Isoproterenol ( ISO )-treated lysates were used as positive control for VASP phosphorylation.
    Dde, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde/product/Millipore
    Average 94 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    dde - by Bioz Stars, 2020-05
    94/100 stars
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    ddx 1  (Abcam)
    86
    Abcam ddx 1
    <t>DDE</t> activates cell signaling proteins MAPK and VASP in prostate cancer cells. LNCaP ( A ) and LAPC4 ( B ) cells were cultured in the absence of steroid for 24 h, and then either vehicle control (0.1% ethanol), 10 −10 mol/L <t>DHT,</t> or 10 −11 mol/L DDE was added. Following 40 or 120 min, cell lysates were collected and subjected to SDS-PAGE followed by immunoblotting for total ERK1/2, p-ERK1/2, or lamin B (loading control). C. LNCaP and LAPC4 cells were cultured in the absence of steroid for 24 h before treating with either 0.1% ethanol, 10 −10 mol/L DHT, or 10 −11 mol/L DDE. C. Following 10-min incubation, cells were harvested and the cell lysates were subjected to SDS-PAGE for expression analysis of phosphorylated VASP ( p-VASP ) or lamin B (loading control). Isoproterenol ( ISO )-treated lysates were used as positive control for VASP phosphorylation.
    Ddx 1, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ddx 1/product/Abcam
    Average 86 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    ddx 1 - by Bioz Stars, 2020-05
    86/100 stars
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    86
    Beckman Coulter ddx 880
    <t>DDE</t> activates cell signaling proteins MAPK and VASP in prostate cancer cells. LNCaP ( A ) and LAPC4 ( B ) cells were cultured in the absence of steroid for 24 h, and then either vehicle control (0.1% ethanol), 10 −10 mol/L <t>DHT,</t> or 10 −11 mol/L DDE was added. Following 40 or 120 min, cell lysates were collected and subjected to SDS-PAGE followed by immunoblotting for total ERK1/2, p-ERK1/2, or lamin B (loading control). C. LNCaP and LAPC4 cells were cultured in the absence of steroid for 24 h before treating with either 0.1% ethanol, 10 −10 mol/L DHT, or 10 −11 mol/L DDE. C. Following 10-min incubation, cells were harvested and the cell lysates were subjected to SDS-PAGE for expression analysis of phosphorylated VASP ( p-VASP ) or lamin B (loading control). Isoproterenol ( ISO )-treated lysates were used as positive control for VASP phosphorylation.
    Ddx 880, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ddx 880/product/Beckman Coulter
    Average 86 stars, based on 3 article reviews
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    ddx 880 - by Bioz Stars, 2020-05
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    90
    Millipore 4 4 dde
    Scatterplots of <t>4,4′-DDE</t> blood concentrations versus loggerhead sea turtle lymphocyte proliferation (SI) responses stimulated for 5 days with 5 μg/mL PHA ( A; r S = 0.431; p = 0.084) and 0.8 μg/mL PDB ( B; r S = 0.507; p = 0.038). SI = cpm of mitogen-stimulated cells/cpm of unstimulated cells. Linear trend lines demonstrate the positive relationships determined using Spearman rank correlations.
    4 4 Dde, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4 4 dde/product/Millipore
    Average 90 stars, based on 6 article reviews
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    4 4 dde - by Bioz Stars, 2020-05
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    90
    TaKaRa dde i
    Schematic representation of primer design for detection of PML-RAR α mRNA by <t>RT-LAMP</t> and cutting sites of <t>Dde</t> I restriction enzyme assay. Exon numbers of the PML gene (dark) and the RAR α gene (light) are shown. PML , promyelocytic leukemia ; RAR α, retinoic acid receptor α; RT-LAMP, reverse transcription loop-mediated isothermal amplification.
    Dde I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde i/product/TaKaRa
    Average 90 stars, based on 24 article reviews
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    dde i - by Bioz Stars, 2020-05
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    90
    Promega dde i
    Diagrams representing restriction patterns of 16S rRNA gene digested with <t>Dde</t> I ( a ), Hha I ( b ) or <t>Rsa</t> I ( c ). First column in each diagram corresponds to the banding pattern for the 1 Kb ladder
    Dde I, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 86 article reviews
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    dde i - by Bioz Stars, 2020-05
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    dde i  (Roche)
    90
    Roche dde i
    Dendrogram based on restriction profiles of <t>fla</t> A gene digested with <t>Dde</t> I of 163 Campylobacter isolates from 5 turkey farms (FI–FV—farm 1–5)
    Dde I, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde i/product/Roche
    Average 90 stars, based on 59 article reviews
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    dde i - by Bioz Stars, 2020-05
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    90
    Thermo Fisher dde i
    Fla typing of inoculation strains and strains isolated from infected chicks. The <t>flaA</t> gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.
    Dde I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde i/product/Thermo Fisher
    Average 90 stars, based on 89 article reviews
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    dde i - by Bioz Stars, 2020-05
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    90
    Toyobo dde i
    Fla typing of inoculation strains and strains isolated from infected chicks. The <t>flaA</t> gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.
    Dde I, supplied by Toyobo, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde i/product/Toyobo
    Average 90 stars, based on 6 article reviews
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    dde i - by Bioz Stars, 2020-05
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    93
    Millipore dde biotin azide linker
    Fla typing of inoculation strains and strains isolated from infected chicks. The <t>flaA</t> gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.
    Dde Biotin Azide Linker, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde biotin azide linker/product/Millipore
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dde biotin azide linker - by Bioz Stars, 2020-05
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    92
    Millipore dde oh
    Fla typing of inoculation strains and strains isolated from infected chicks. The <t>flaA</t> gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.
    Dde Oh, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde oh/product/Millipore
    Average 92 stars, based on 2 article reviews
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    dde oh - by Bioz Stars, 2020-05
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    90
    LGC Standards GmbH dde
    Fla typing of inoculation strains and strains isolated from infected chicks. The <t>flaA</t> gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.
    Dde, supplied by LGC Standards GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde/product/LGC Standards GmbH
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dde - by Bioz Stars, 2020-05
    90/100 stars
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    91
    Microsoft dde
    Fla typing of inoculation strains and strains isolated from infected chicks. The <t>flaA</t> gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.
    Dde, supplied by Microsoft, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde/product/Microsoft
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    dde - by Bioz Stars, 2020-05
    91/100 stars
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    91
    PhaseSpace Inc dde
    Fla typing of inoculation strains and strains isolated from infected chicks. The <t>flaA</t> gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.
    Dde, supplied by PhaseSpace Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde/product/PhaseSpace Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dde - by Bioz Stars, 2020-05
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    91
    Tokyo Chemical Industry dde
    Fla typing of inoculation strains and strains isolated from infected chicks. The <t>flaA</t> gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.
    Dde, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde/product/Tokyo Chemical Industry
    Average 91 stars, based on 1 article reviews
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    dde - by Bioz Stars, 2020-05
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    88
    Abcam rabbit polyclonal ddx 4
    Fla typing of inoculation strains and strains isolated from infected chicks. The <t>flaA</t> gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.
    Rabbit Polyclonal Ddx 4, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal ddx 4/product/Abcam
    Average 88 stars, based on 13 article reviews
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    rabbit polyclonal ddx 4 - by Bioz Stars, 2020-05
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    86
    Cell Signaling Technology Inc ddx 3
    Fla typing of inoculation strains and strains isolated from infected chicks. The <t>flaA</t> gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.
    Ddx 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ddx 3/product/Cell Signaling Technology Inc
    Average 86 stars, based on 6 article reviews
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    ddx 3 - by Bioz Stars, 2020-05
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    91
    Cerilliant Corporation dde
    Fla typing of inoculation strains and strains isolated from infected chicks. The <t>flaA</t> gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.
    Dde, supplied by Cerilliant Corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde/product/Cerilliant Corporation
    Average 91 stars, based on 1 article reviews
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    dde - by Bioz Stars, 2020-05
    91/100 stars
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    dde  (Supelco)
    91
    Supelco dde
    Fla typing of inoculation strains and strains isolated from infected chicks. The <t>flaA</t> gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.
    Dde, supplied by Supelco, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dde/product/Supelco
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    dde - by Bioz Stars, 2020-05
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    Image Search Results


    DDE activates cell signaling proteins MAPK and VASP in prostate cancer cells. LNCaP ( A ) and LAPC4 ( B ) cells were cultured in the absence of steroid for 24 h, and then either vehicle control (0.1% ethanol), 10 −10 mol/L DHT, or 10 −11 mol/L DDE was added. Following 40 or 120 min, cell lysates were collected and subjected to SDS-PAGE followed by immunoblotting for total ERK1/2, p-ERK1/2, or lamin B (loading control). C. LNCaP and LAPC4 cells were cultured in the absence of steroid for 24 h before treating with either 0.1% ethanol, 10 −10 mol/L DHT, or 10 −11 mol/L DDE. C. Following 10-min incubation, cells were harvested and the cell lysates were subjected to SDS-PAGE for expression analysis of phosphorylated VASP ( p-VASP ) or lamin B (loading control). Isoproterenol ( ISO )-treated lysates were used as positive control for VASP phosphorylation.

    Journal: Molecular cancer research : MCR

    Article Title: 2,2-Bis(4-Chlorophenyl)-1,1-Dichloroethylene Stimulates Androgen Independence in Prostate Cancer Cells through Combinatorial Activation of Mutant Androgen Receptor and Mitogen-Activated Protein Kinase Pathways

    doi: 10.1158/1541-7786.MCR-07-2166

    Figure Lengend Snippet: DDE activates cell signaling proteins MAPK and VASP in prostate cancer cells. LNCaP ( A ) and LAPC4 ( B ) cells were cultured in the absence of steroid for 24 h, and then either vehicle control (0.1% ethanol), 10 −10 mol/L DHT, or 10 −11 mol/L DDE was added. Following 40 or 120 min, cell lysates were collected and subjected to SDS-PAGE followed by immunoblotting for total ERK1/2, p-ERK1/2, or lamin B (loading control). C. LNCaP and LAPC4 cells were cultured in the absence of steroid for 24 h before treating with either 0.1% ethanol, 10 −10 mol/L DHT, or 10 −11 mol/L DDE. C. Following 10-min incubation, cells were harvested and the cell lysates were subjected to SDS-PAGE for expression analysis of phosphorylated VASP ( p-VASP ) or lamin B (loading control). Isoproterenol ( ISO )-treated lysates were used as positive control for VASP phosphorylation.

    Article Snippet: DHT, DDT, DDE, resveratrol, coumestrol, and cadmium were obtained from Sigma-Aldrich.

    Techniques: Cell Culture, SDS Page, Incubation, Expressing, Positive Control

    DDE induces proliferation in AR-T877A – expressing prostate cancer cells. 22Rv1 ( A ) or LNCaP ( B ) cells were cultured in the absence of steroid for 24 h. Then, either vehicle control (0.1% ethanol), 10 −10 mol/L DHT, or increasing doses of DDE (10 −11 , 10 −8 , or 10 −5 mol/L) were added for 24 h. Cells were labeled with BrdUrd for 16 h and BrdUrd incorporation was monitored via indirect immunofluorescence. Dotted line, the level of BrdUrd incorporation in the absence of ligand (vehicle control) for each cell type was set to 1. C. LNCaP cells were cultured in the absence of steroid for 24 h before the addition of ligand [ethanol control, 10 −10 mol/L DHT, or DDE (10 −11 , 10 −8 , or 10 −5 mol/L)] for the indicated times (24, 48, 72, 96, or 120 h) with fresh ligand added every 48 h. At each time point, cells were collected and cell number and viability were determined via trypan blue exclusion. Experiments were repeated with three biological replicates, each analyzed in triplicate. *, P

    Journal: Molecular cancer research : MCR

    Article Title: 2,2-Bis(4-Chlorophenyl)-1,1-Dichloroethylene Stimulates Androgen Independence in Prostate Cancer Cells through Combinatorial Activation of Mutant Androgen Receptor and Mitogen-Activated Protein Kinase Pathways

    doi: 10.1158/1541-7786.MCR-07-2166

    Figure Lengend Snippet: DDE induces proliferation in AR-T877A – expressing prostate cancer cells. 22Rv1 ( A ) or LNCaP ( B ) cells were cultured in the absence of steroid for 24 h. Then, either vehicle control (0.1% ethanol), 10 −10 mol/L DHT, or increasing doses of DDE (10 −11 , 10 −8 , or 10 −5 mol/L) were added for 24 h. Cells were labeled with BrdUrd for 16 h and BrdUrd incorporation was monitored via indirect immunofluorescence. Dotted line, the level of BrdUrd incorporation in the absence of ligand (vehicle control) for each cell type was set to 1. C. LNCaP cells were cultured in the absence of steroid for 24 h before the addition of ligand [ethanol control, 10 −10 mol/L DHT, or DDE (10 −11 , 10 −8 , or 10 −5 mol/L)] for the indicated times (24, 48, 72, 96, or 120 h) with fresh ligand added every 48 h. At each time point, cells were collected and cell number and viability were determined via trypan blue exclusion. Experiments were repeated with three biological replicates, each analyzed in triplicate. *, P

    Article Snippet: DHT, DDT, DDE, resveratrol, coumestrol, and cadmium were obtained from Sigma-Aldrich.

    Techniques: Expressing, Cell Culture, Labeling, Immunofluorescence

    DDE uses MAPK and mutant AR pathways for induction of cell cycle progression in LNCaP cells. LNCaP cells were cultured in the absence of steroid (0.1% DMSO vehicle control; white columns ) or in the presence of 10 −6 mol/L Casodex ( CSDX; black striped columns ), 5 ×10 −6 mol/L Casodex ( black crisscrossed columns ), 10 −6 mol/L MEK inhibitor (U0126; gray columns ), or combined 10 −6 mol/L Casodex and 10 −6 mol/L U0126 ( black columns ). Following 24 h of pretreatment, cells were stimulated with vehicle control (ethanol), 10 −10 mol/L DHT, or 10 −11 mol/L DDE for 24 h and labeled with BrdUrd during the last 16 h of treatment. BrdUrd incorporation for each ligand in the absence of inhibitory challenge was set to 100%. Experiments were done in triplicate. Columns, average; bars, SD. *, P

    Journal: Molecular cancer research : MCR

    Article Title: 2,2-Bis(4-Chlorophenyl)-1,1-Dichloroethylene Stimulates Androgen Independence in Prostate Cancer Cells through Combinatorial Activation of Mutant Androgen Receptor and Mitogen-Activated Protein Kinase Pathways

    doi: 10.1158/1541-7786.MCR-07-2166

    Figure Lengend Snippet: DDE uses MAPK and mutant AR pathways for induction of cell cycle progression in LNCaP cells. LNCaP cells were cultured in the absence of steroid (0.1% DMSO vehicle control; white columns ) or in the presence of 10 −6 mol/L Casodex ( CSDX; black striped columns ), 5 ×10 −6 mol/L Casodex ( black crisscrossed columns ), 10 −6 mol/L MEK inhibitor (U0126; gray columns ), or combined 10 −6 mol/L Casodex and 10 −6 mol/L U0126 ( black columns ). Following 24 h of pretreatment, cells were stimulated with vehicle control (ethanol), 10 −10 mol/L DHT, or 10 −11 mol/L DDE for 24 h and labeled with BrdUrd during the last 16 h of treatment. BrdUrd incorporation for each ligand in the absence of inhibitory challenge was set to 100%. Experiments were done in triplicate. Columns, average; bars, SD. *, P

    Article Snippet: DHT, DDT, DDE, resveratrol, coumestrol, and cadmium were obtained from Sigma-Aldrich.

    Techniques: Mutagenesis, Cell Culture, Labeling

    DDE induces mutant AR transcriptional activity in CV1 cells. CV1 cells were transfected in the absence of androgen with plasmids encoding wtAR or mutant AR (T877A or H874Y), ARR2-luciferase reporter, along with cytomegalovirus-β-galactosidase as an internal transfection control. After transfection, the cells were treated with either ethanol ( EtOH ), DHT, or DDE for 24 h. Cells were harvested using trypsin, lysed using reporter lysis buffer, and analyzed for luciferase activity. The luciferase/β-galactosidase ratio for endogenous ligand DHT was set to 100. Experiments were done with at least three independent replicates. *, P

    Journal: Molecular cancer research : MCR

    Article Title: 2,2-Bis(4-Chlorophenyl)-1,1-Dichloroethylene Stimulates Androgen Independence in Prostate Cancer Cells through Combinatorial Activation of Mutant Androgen Receptor and Mitogen-Activated Protein Kinase Pathways

    doi: 10.1158/1541-7786.MCR-07-2166

    Figure Lengend Snippet: DDE induces mutant AR transcriptional activity in CV1 cells. CV1 cells were transfected in the absence of androgen with plasmids encoding wtAR or mutant AR (T877A or H874Y), ARR2-luciferase reporter, along with cytomegalovirus-β-galactosidase as an internal transfection control. After transfection, the cells were treated with either ethanol ( EtOH ), DHT, or DDE for 24 h. Cells were harvested using trypsin, lysed using reporter lysis buffer, and analyzed for luciferase activity. The luciferase/β-galactosidase ratio for endogenous ligand DHT was set to 100. Experiments were done with at least three independent replicates. *, P

    Article Snippet: DHT, DDT, DDE, resveratrol, coumestrol, and cadmium were obtained from Sigma-Aldrich.

    Techniques: Mutagenesis, Activity Assay, Transfection, Luciferase, Lysis

    Effect of p,p ′-DDE and o,p ′-DDE (both at 10 μM) on caspase-3 activity ( a , b ) and LDH release ( c , d ) in RXRα or RXRβ siRNA-transfected hippocampal cells. The effectiveness of mRNA silencing was verified using qPCR ( e ). Primary hippocampal cultures were transfected with 50 nM RXRα or RXRβ siRNAs in INTERFERin™-containing medium without antibiotics for 6 h. The results were normalized to the absorbance in vehicle-treated cells and expressed as a percentage of the control, either in siRNA-transfected or non-transfected cells ( a – d ). mRNA silencing was presented as a fold of the control non-transfected cells ( e ). Each bar represents the mean ± SEM of three to four independent experiments. The number of replicates in each experiment ranged from 5 to 8. *** p

    Journal: Neurotoxicity Research

    Article Title: The Crucial Involvement of Retinoid X Receptors in DDE Neurotoxicity

    doi: 10.1007/s12640-015-9572-6

    Figure Lengend Snippet: Effect of p,p ′-DDE and o,p ′-DDE (both at 10 μM) on caspase-3 activity ( a , b ) and LDH release ( c , d ) in RXRα or RXRβ siRNA-transfected hippocampal cells. The effectiveness of mRNA silencing was verified using qPCR ( e ). Primary hippocampal cultures were transfected with 50 nM RXRα or RXRβ siRNAs in INTERFERin™-containing medium without antibiotics for 6 h. The results were normalized to the absorbance in vehicle-treated cells and expressed as a percentage of the control, either in siRNA-transfected or non-transfected cells ( a – d ). mRNA silencing was presented as a fold of the control non-transfected cells ( e ). Each bar represents the mean ± SEM of three to four independent experiments. The number of replicates in each experiment ranged from 5 to 8. *** p

    Article Snippet: Measurement of Global DNA Methylation Global DNA methylation shifts at 24 h after treatment with p,p ′-DDE or o,p ′-DDE were measured in hippocampal cells using a specific ELISA-based format kit (Imprint® Methylated DNA Quantification—Sigma-Aldrich; St. Louis, MO, USA).

    Techniques: Activity Assay, Transfection, Real-time Polymerase Chain Reaction

    Influence of p,p ′-DDE and o,p ′-DDE on global DNA methylation at 7 DIV hippocampal cultures. Primary hippocampal cultures were treated with p,p ′-DDE or o,p ′-DDE (both at 10 μM) and total DNA was extracted from hippocampal cells at 24 h post-treatment, followed by ELISA. Each bar represents the mean of three independent experiments ± SEM. The number of replicates in each experiment ranged from 2 to 3. ** p

    Journal: Neurotoxicity Research

    Article Title: The Crucial Involvement of Retinoid X Receptors in DDE Neurotoxicity

    doi: 10.1007/s12640-015-9572-6

    Figure Lengend Snippet: Influence of p,p ′-DDE and o,p ′-DDE on global DNA methylation at 7 DIV hippocampal cultures. Primary hippocampal cultures were treated with p,p ′-DDE or o,p ′-DDE (both at 10 μM) and total DNA was extracted from hippocampal cells at 24 h post-treatment, followed by ELISA. Each bar represents the mean of three independent experiments ± SEM. The number of replicates in each experiment ranged from 2 to 3. ** p

    Article Snippet: Measurement of Global DNA Methylation Global DNA methylation shifts at 24 h after treatment with p,p ′-DDE or o,p ′-DDE were measured in hippocampal cells using a specific ELISA-based format kit (Imprint® Methylated DNA Quantification—Sigma-Aldrich; St. Louis, MO, USA).

    Techniques: DNA Methylation Assay, Enzyme-linked Immunosorbent Assay

    Impact of the RXR antagonist on p,p ′-DDE and o,p ′-DDE–induced on caspase-3 activity ( a ) and LDH release ( b ) at 7 DIV hippocampal cultures. Primary hippocampal cultures were treated with p,p ′-DDE or o,p ′-DDE (10, 100 μM) for 6 h. The RXR antagonist HX 531 (0.1 μM) was added into the culture media approximately 45–60 min before DDE was added. The results were normalized to the absorbency in vehicle-treated cells and expressed as a percentage of control. Each bar represents the mean of three to four independent experiments ± SEM. The number of replicates in each experiment ranged from 5 to 8. * p

    Journal: Neurotoxicity Research

    Article Title: The Crucial Involvement of Retinoid X Receptors in DDE Neurotoxicity

    doi: 10.1007/s12640-015-9572-6

    Figure Lengend Snippet: Impact of the RXR antagonist on p,p ′-DDE and o,p ′-DDE–induced on caspase-3 activity ( a ) and LDH release ( b ) at 7 DIV hippocampal cultures. Primary hippocampal cultures were treated with p,p ′-DDE or o,p ′-DDE (10, 100 μM) for 6 h. The RXR antagonist HX 531 (0.1 μM) was added into the culture media approximately 45–60 min before DDE was added. The results were normalized to the absorbency in vehicle-treated cells and expressed as a percentage of control. Each bar represents the mean of three to four independent experiments ± SEM. The number of replicates in each experiment ranged from 5 to 8. * p

    Article Snippet: Measurement of Global DNA Methylation Global DNA methylation shifts at 24 h after treatment with p,p ′-DDE or o,p ′-DDE were measured in hippocampal cells using a specific ELISA-based format kit (Imprint® Methylated DNA Quantification—Sigma-Aldrich; St. Louis, MO, USA).

    Techniques: Activity Assay

    Effect of p,p ′-DDE and o,p ′-DDE (both at 10 μM) on the mRNA expression levels of Rxrα , Rxrβ , and Rxrγ in hippocampal cultures at 7 DIV. The extraction of total RNA at 6 h post-treatment from the hippocampal cells was followed by reverse transcription (RT) and quantitative polymerase chain reaction (qPCR). The products of the RT reaction were amplified using TaqMan probes and primers corresponding to the specific genes. Hprt was used as a reference gene. Each bar represents the mean ± SEM of three independent experiments. The number of replicates for each experiment ranged from 2 to 3, ** p

    Journal: Neurotoxicity Research

    Article Title: The Crucial Involvement of Retinoid X Receptors in DDE Neurotoxicity

    doi: 10.1007/s12640-015-9572-6

    Figure Lengend Snippet: Effect of p,p ′-DDE and o,p ′-DDE (both at 10 μM) on the mRNA expression levels of Rxrα , Rxrβ , and Rxrγ in hippocampal cultures at 7 DIV. The extraction of total RNA at 6 h post-treatment from the hippocampal cells was followed by reverse transcription (RT) and quantitative polymerase chain reaction (qPCR). The products of the RT reaction were amplified using TaqMan probes and primers corresponding to the specific genes. Hprt was used as a reference gene. Each bar represents the mean ± SEM of three independent experiments. The number of replicates for each experiment ranged from 2 to 3, ** p

    Article Snippet: Measurement of Global DNA Methylation Global DNA methylation shifts at 24 h after treatment with p,p ′-DDE or o,p ′-DDE were measured in hippocampal cells using a specific ELISA-based format kit (Imprint® Methylated DNA Quantification—Sigma-Aldrich; St. Louis, MO, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Amplification

    Effects of p,p ′-DDE and o,p ′-DDE on protein levels of RXRα and RXRβ in mouse hippocampal cultures at 7 DIV. Hippocampal cells were cultured for 7 DIV and then treated for 24 h with p,p ′-DDE (10 μM) or o,p ′-DDE (10 μM). The concentrations of the receptors were measured using specific ELISAs and presented as pg of RXRα or RXRβ per μg of total protein. For Western blot analyses, protein samples were denatured, electrophoretically separated, transferred to PVDF membrane, and subjected to immunolabeling. Signals were developed by chemiluminescence (ECL) and visualized with Luminescent Image Analyzer Fuji-Las 4000 (Fuji, Japan). Immunoreactive bands were quantified using an image analyzer (ScienceLab, MultiGauge V3.0), and the relative protein levels of RXRα and RXRβ were presented as a percentage of the control. Each bar or value represents the mean of three independent experiments ± SEM. The number of replicates in each experiment ranged from 2 to 3. * p

    Journal: Neurotoxicity Research

    Article Title: The Crucial Involvement of Retinoid X Receptors in DDE Neurotoxicity

    doi: 10.1007/s12640-015-9572-6

    Figure Lengend Snippet: Effects of p,p ′-DDE and o,p ′-DDE on protein levels of RXRα and RXRβ in mouse hippocampal cultures at 7 DIV. Hippocampal cells were cultured for 7 DIV and then treated for 24 h with p,p ′-DDE (10 μM) or o,p ′-DDE (10 μM). The concentrations of the receptors were measured using specific ELISAs and presented as pg of RXRα or RXRβ per μg of total protein. For Western blot analyses, protein samples were denatured, electrophoretically separated, transferred to PVDF membrane, and subjected to immunolabeling. Signals were developed by chemiluminescence (ECL) and visualized with Luminescent Image Analyzer Fuji-Las 4000 (Fuji, Japan). Immunoreactive bands were quantified using an image analyzer (ScienceLab, MultiGauge V3.0), and the relative protein levels of RXRα and RXRβ were presented as a percentage of the control. Each bar or value represents the mean of three independent experiments ± SEM. The number of replicates in each experiment ranged from 2 to 3. * p

    Article Snippet: Measurement of Global DNA Methylation Global DNA methylation shifts at 24 h after treatment with p,p ′-DDE or o,p ′-DDE were measured in hippocampal cells using a specific ELISA-based format kit (Imprint® Methylated DNA Quantification—Sigma-Aldrich; St. Louis, MO, USA).

    Techniques: Cell Culture, Western Blot, Immunolabeling

    Time-course effects of p,p ′-DDE and o,p ′-DDE (0.1, 1, 10, and 100 μM) on caspase-3 activity ( a ) and LDH release ( b ) in primary cultures of mouse hippocampal cells at 7 DIV. Cells were treated with p,p ′-DDE or o,p ′-DDE for 6 and 24 h. The results are presented as a percentage of the control. Each bar represents the mean of three to four independent experiments ± SEM. The number of replicates in each experiment ranged from 5 to 8. * p

    Journal: Neurotoxicity Research

    Article Title: The Crucial Involvement of Retinoid X Receptors in DDE Neurotoxicity

    doi: 10.1007/s12640-015-9572-6

    Figure Lengend Snippet: Time-course effects of p,p ′-DDE and o,p ′-DDE (0.1, 1, 10, and 100 μM) on caspase-3 activity ( a ) and LDH release ( b ) in primary cultures of mouse hippocampal cells at 7 DIV. Cells were treated with p,p ′-DDE or o,p ′-DDE for 6 and 24 h. The results are presented as a percentage of the control. Each bar represents the mean of three to four independent experiments ± SEM. The number of replicates in each experiment ranged from 5 to 8. * p

    Article Snippet: Measurement of Global DNA Methylation Global DNA methylation shifts at 24 h after treatment with p,p ′-DDE or o,p ′-DDE were measured in hippocampal cells using a specific ELISA-based format kit (Imprint® Methylated DNA Quantification—Sigma-Aldrich; St. Louis, MO, USA).

    Techniques: Activity Assay

    Time-course effects of p,p ′-DDE and o,p ′-DDE (0.1, 1, 10, 100 μM) on caspase-3 activity ( a ) and LDH release ( b ) in primary cultures of mouse neocortical cells at 7 DIV. The cells were treated with p,p ′-DDE or o,p ′-DDE for 6 and 24 h. The results are presented as a percentage of the control. Each bar represents the mean of three to four independent experiments ± SEM. The number of replicates in each experiment ranged from 5 to 8. * p

    Journal: Neurotoxicity Research

    Article Title: The Crucial Involvement of Retinoid X Receptors in DDE Neurotoxicity

    doi: 10.1007/s12640-015-9572-6

    Figure Lengend Snippet: Time-course effects of p,p ′-DDE and o,p ′-DDE (0.1, 1, 10, 100 μM) on caspase-3 activity ( a ) and LDH release ( b ) in primary cultures of mouse neocortical cells at 7 DIV. The cells were treated with p,p ′-DDE or o,p ′-DDE for 6 and 24 h. The results are presented as a percentage of the control. Each bar represents the mean of three to four independent experiments ± SEM. The number of replicates in each experiment ranged from 5 to 8. * p

    Article Snippet: Measurement of Global DNA Methylation Global DNA methylation shifts at 24 h after treatment with p,p ′-DDE or o,p ′-DDE were measured in hippocampal cells using a specific ELISA-based format kit (Imprint® Methylated DNA Quantification—Sigma-Aldrich; St. Louis, MO, USA).

    Techniques: Activity Assay

    Time-course effects of p,p ′-DDE and o,p ′-DDE (0.1, 1, 10, 100 μM) on caspase-3 activity ( a ) and LDH release ( b ) in primary cultures of mouse cerebellar cells at 7 DIV. The cells were treated with p,p ′-DDE or o,p ′-DDE for 6 and 24 h. The results are presented as a percentage of the control. Each bar represents the mean of three to four independent experiments ± SEM. The number of replicates in each experiment ranged from 5 to 8. * p

    Journal: Neurotoxicity Research

    Article Title: The Crucial Involvement of Retinoid X Receptors in DDE Neurotoxicity

    doi: 10.1007/s12640-015-9572-6

    Figure Lengend Snippet: Time-course effects of p,p ′-DDE and o,p ′-DDE (0.1, 1, 10, 100 μM) on caspase-3 activity ( a ) and LDH release ( b ) in primary cultures of mouse cerebellar cells at 7 DIV. The cells were treated with p,p ′-DDE or o,p ′-DDE for 6 and 24 h. The results are presented as a percentage of the control. Each bar represents the mean of three to four independent experiments ± SEM. The number of replicates in each experiment ranged from 5 to 8. * p

    Article Snippet: Measurement of Global DNA Methylation Global DNA methylation shifts at 24 h after treatment with p,p ′-DDE or o,p ′-DDE were measured in hippocampal cells using a specific ELISA-based format kit (Imprint® Methylated DNA Quantification—Sigma-Aldrich; St. Louis, MO, USA).

    Techniques: Activity Assay

    Scatterplots of 4,4′-DDE blood concentrations versus loggerhead sea turtle lymphocyte proliferation (SI) responses stimulated for 5 days with 5 μg/mL PHA ( A; r S = 0.431; p = 0.084) and 0.8 μg/mL PDB ( B; r S = 0.507; p = 0.038). SI = cpm of mitogen-stimulated cells/cpm of unstimulated cells. Linear trend lines demonstrate the positive relationships determined using Spearman rank correlations.

    Journal: Environmental Health Perspectives

    Article Title: Effects of Organochlorine Contaminants on Loggerhead Sea Turtle Immunity: Comparison of a Correlative Field Study and In Vitro Exposure Experiments

    doi: 10.1289/ehp.8143

    Figure Lengend Snippet: Scatterplots of 4,4′-DDE blood concentrations versus loggerhead sea turtle lymphocyte proliferation (SI) responses stimulated for 5 days with 5 μg/mL PHA ( A; r S = 0.431; p = 0.084) and 0.8 μg/mL PDB ( B; r S = 0.507; p = 0.038). SI = cpm of mitogen-stimulated cells/cpm of unstimulated cells. Linear trend lines demonstrate the positive relationships determined using Spearman rank correlations.

    Article Snippet: In 2004, neat Aroclor 1254 (Supelco, Bellefonte, PA) and 4,4′-DDE (Aldrich Chemical Co., Milwaukee, WI) were weighed and diluted in sterile DMSO to make stock solutions.

    Techniques:

    The effect of a 5-day in vitro exposure to 4,4′-DDE on loggerhead sea turtle lymphocyte proliferation (SI) responses stimulated by 5 μg/mL PHA ( A ) and 0.2 μg/mL PDB ( B ). Data are shown as mean ± SE of the percentage of the SI measured in the control (no DMSO or 4,4′-DDE) for each turtle. Sample sizes are 8 or 16 depending on the treatment group. The x -axis crosses the y -axis at the percentage of the control value for the wells receiving only DMSO. The mean ± SE SI for the DMSO controls in the PHA and PDB experiments was 96.6 ± 15.0 and 172 ± 39, respectively. Vertical dashed lines indicate the range of 4,4′-DDE concentrations measured in the blood of 17 loggerhead sea turtles used in the correlative field study. *Significantly different from the DMSO control (ANOVA with log-transformed data, Dunnet’s multiple comparison test; p

    Journal: Environmental Health Perspectives

    Article Title: Effects of Organochlorine Contaminants on Loggerhead Sea Turtle Immunity: Comparison of a Correlative Field Study and In Vitro Exposure Experiments

    doi: 10.1289/ehp.8143

    Figure Lengend Snippet: The effect of a 5-day in vitro exposure to 4,4′-DDE on loggerhead sea turtle lymphocyte proliferation (SI) responses stimulated by 5 μg/mL PHA ( A ) and 0.2 μg/mL PDB ( B ). Data are shown as mean ± SE of the percentage of the SI measured in the control (no DMSO or 4,4′-DDE) for each turtle. Sample sizes are 8 or 16 depending on the treatment group. The x -axis crosses the y -axis at the percentage of the control value for the wells receiving only DMSO. The mean ± SE SI for the DMSO controls in the PHA and PDB experiments was 96.6 ± 15.0 and 172 ± 39, respectively. Vertical dashed lines indicate the range of 4,4′-DDE concentrations measured in the blood of 17 loggerhead sea turtles used in the correlative field study. *Significantly different from the DMSO control (ANOVA with log-transformed data, Dunnet’s multiple comparison test; p

    Article Snippet: In 2004, neat Aroclor 1254 (Supelco, Bellefonte, PA) and 4,4′-DDE (Aldrich Chemical Co., Milwaukee, WI) were weighed and diluted in sterile DMSO to make stock solutions.

    Techniques: In Vitro, Transformation Assay

    Scatterplots of plasma lysozyme activity versus concentrations of 4,4′-DDE ( A; r S = −0.310, p = 0.038) and ∑chlordanes ( B; r S = −0.368, p = 0.013) measured in the blood of loggerhead sea turtles. Linear trend lines demonstrate the negative relationships determined using Spearman rank correlations.

    Journal: Environmental Health Perspectives

    Article Title: Effects of Organochlorine Contaminants on Loggerhead Sea Turtle Immunity: Comparison of a Correlative Field Study and In Vitro Exposure Experiments

    doi: 10.1289/ehp.8143

    Figure Lengend Snippet: Scatterplots of plasma lysozyme activity versus concentrations of 4,4′-DDE ( A; r S = −0.310, p = 0.038) and ∑chlordanes ( B; r S = −0.368, p = 0.013) measured in the blood of loggerhead sea turtles. Linear trend lines demonstrate the negative relationships determined using Spearman rank correlations.

    Article Snippet: In 2004, neat Aroclor 1254 (Supelco, Bellefonte, PA) and 4,4′-DDE (Aldrich Chemical Co., Milwaukee, WI) were weighed and diluted in sterile DMSO to make stock solutions.

    Techniques: Activity Assay

    Schematic representation of primer design for detection of PML-RAR α mRNA by RT-LAMP and cutting sites of Dde I restriction enzyme assay. Exon numbers of the PML gene (dark) and the RAR α gene (light) are shown. PML , promyelocytic leukemia ; RAR α, retinoic acid receptor α; RT-LAMP, reverse transcription loop-mediated isothermal amplification.

    Journal: Yonago Acta Medica

    Article Title: Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia–Retinoic Acid Receptor α mRNA

    doi:

    Figure Lengend Snippet: Schematic representation of primer design for detection of PML-RAR α mRNA by RT-LAMP and cutting sites of Dde I restriction enzyme assay. Exon numbers of the PML gene (dark) and the RAR α gene (light) are shown. PML , promyelocytic leukemia ; RAR α, retinoic acid receptor α; RT-LAMP, reverse transcription loop-mediated isothermal amplification.

    Article Snippet: The specificity of LAMP was confirmed by restriction enzyme digestion using Dde I (Takara Bio, Otsu, Japan) under the following conditions: 5 μL LAMP product was mixed with 2 μL of 10 × K buffer (Takara Bio), 1 μL Dde I, with distilled water added to obtain a final volume of 20 μL, followed by incubation at 37 °C for 120 min. Dde I was inactivated at 70 °C for 15 min and the digestion products (10-μL aliquots) were mixed with loading buffer (Nippon Gene, Toyama, Japan) and separated by 1.5% agarose gel electrophoresis (100 V, 35 min) with 0.005% ethidium bromide using a Mupid mini gel electrophoresis system (Advance, Tokyo, Japan), with 5 μL Gene Ladder 100 (Nippon Gene) used as a molecular size marker.

    Techniques: Enzymatic Assay, Amplification

    Dde I restriction enzyme digestion of RT-LAMP products. Products of bcr1, bcr2, and bcr3 plasmid DNA amplification were incubated with (+) or without (−) Dde I at 37 °C for 120 min, followed by deactivation at 70 °C for 15 min and separation by 1.5% agarose gel electrophoresis (100 V, 35 min). The amounts of bcr1, bcr2 and bcr3 plasmid DNAs were 8.8, 7.8, and 8.0 × 10 6 copies/tube, respectively. The expected sizes of digested RT-LAMP products are the followings: 468 bp, about 234 bp (stem and loop structure) and 83 bp for bcr1; 232 bp, about 116 bp (stem and loop structure) and 83 bp for bcr2; and 139 bp and 121 bp (merged in Figure) for bcr3. DW, distilled water (negative control); M, 100-bp ladder size marker; neg RNA, IVS-0035 RNA (negative control); RT-LAMP, reverse transcription loop-mediated isothermal amplification.

    Journal: Yonago Acta Medica

    Article Title: Development of Reverse Transcription Loop-Mediated Isothermal Amplification for Simple and Rapid Detection of Promyelocytic Leukemia–Retinoic Acid Receptor α mRNA

    doi:

    Figure Lengend Snippet: Dde I restriction enzyme digestion of RT-LAMP products. Products of bcr1, bcr2, and bcr3 plasmid DNA amplification were incubated with (+) or without (−) Dde I at 37 °C for 120 min, followed by deactivation at 70 °C for 15 min and separation by 1.5% agarose gel electrophoresis (100 V, 35 min). The amounts of bcr1, bcr2 and bcr3 plasmid DNAs were 8.8, 7.8, and 8.0 × 10 6 copies/tube, respectively. The expected sizes of digested RT-LAMP products are the followings: 468 bp, about 234 bp (stem and loop structure) and 83 bp for bcr1; 232 bp, about 116 bp (stem and loop structure) and 83 bp for bcr2; and 139 bp and 121 bp (merged in Figure) for bcr3. DW, distilled water (negative control); M, 100-bp ladder size marker; neg RNA, IVS-0035 RNA (negative control); RT-LAMP, reverse transcription loop-mediated isothermal amplification.

    Article Snippet: The specificity of LAMP was confirmed by restriction enzyme digestion using Dde I (Takara Bio, Otsu, Japan) under the following conditions: 5 μL LAMP product was mixed with 2 μL of 10 × K buffer (Takara Bio), 1 μL Dde I, with distilled water added to obtain a final volume of 20 μL, followed by incubation at 37 °C for 120 min. Dde I was inactivated at 70 °C for 15 min and the digestion products (10-μL aliquots) were mixed with loading buffer (Nippon Gene, Toyama, Japan) and separated by 1.5% agarose gel electrophoresis (100 V, 35 min) with 0.005% ethidium bromide using a Mupid mini gel electrophoresis system (Advance, Tokyo, Japan), with 5 μL Gene Ladder 100 (Nippon Gene) used as a molecular size marker.

    Techniques: Plasmid Preparation, Amplification, Incubation, Agarose Gel Electrophoresis, Negative Control, Marker

    Diagrams representing restriction patterns of 16S rRNA gene digested with Dde I ( a ), Hha I ( b ) or Rsa I ( c ). First column in each diagram corresponds to the banding pattern for the 1 Kb ladder

    Journal: Microbial ecology

    Article Title: Characterization of the Bacterial Diversity in Indo-West Pacific Loliginid and Sepiolid Squid Light Organs

    doi: 10.1007/s00248-012-0099-6

    Figure Lengend Snippet: Diagrams representing restriction patterns of 16S rRNA gene digested with Dde I ( a ), Hha I ( b ) or Rsa I ( c ). First column in each diagram corresponds to the banding pattern for the 1 Kb ladder

    Article Snippet: RFLP analysis was completed as described by Urakawa et al. [ , ] using three restriction endonucleases: Rsa I (5′GTAC3′), Hha I (5′GCGC3′) and Dde I (5′CTNAG3′; Promega Corporation, Madison, WI).

    Techniques:

    Dendrogram based on restriction profiles of fla A gene digested with Dde I of 163 Campylobacter isolates from 5 turkey farms (FI–FV—farm 1–5)

    Journal: Gut Pathogens

    Article Title: Prevalence, genotyping and risk factors of thermophilic Campylobacter spreading in organic turkey farms in Germany

    doi: 10.1186/s13099-016-0108-2

    Figure Lengend Snippet: Dendrogram based on restriction profiles of fla A gene digested with Dde I of 163 Campylobacter isolates from 5 turkey farms (FI–FV—farm 1–5)

    Article Snippet: The fla A amplicon was digested for 18 h at 37 °C with Dde I (Roche Diagnostics GmbH).

    Techniques:

    Fla typing of inoculation strains and strains isolated from infected chicks. The flaA gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.

    Journal: Applied and Environmental Microbiology

    Article Title: Study of the Infectivity of Saline-Stored Campylobacter jejuni for Day-Old Chicks

    doi: 10.1128/AEM.67.5.2388-2392.2001

    Figure Lengend Snippet: Fla typing of inoculation strains and strains isolated from infected chicks. The flaA gene was amplified by PCR and digested with either (A) Alu I or (B) Dde I. M, molecular size marker (100-bp DNA ladder); lane 1, Pen-6 inoculation strain; lanes 2 and 3, isolates from chickens colonized with the Pen-6 strain; lane 4, Kl-5133 inoculation strain; lanes 5 and 6, isolates from chickens colonized with the Kl-5133 strain; lane 7, G97-76595 inoculation strain; lanes 8 and 9, isolates from chickens colonized with the G97-76595 strain. Lane numbers refer to the same information in both panels A and B.

    Article Snippet: Samples of the flaA PCR product were subsequently digested with Dde I (Life Technologies, Rockville, Md.) and Alu I (Boehringer GmbH, Mannheim, Germany) separately, and the digests were analyzed by 3% agarose gel electrophoresis with a 100-bp DNA ladder (Life Technologies) as the molecular size marker.

    Techniques: Isolation, Infection, Amplification, Polymerase Chain Reaction, Marker