Journal: Folia microbiologica

Article Title: Unusual oral mucosal microbiota after hematopoietic cell transplantation with glycopeptide antibiotics: potential association with pathophysiology of oral mucositis.

doi: 10.1007/s12223-018-0596-1

Figure Lengend Snippet: Fig. 2 DGGE profiles of amplified 16S rDNA from mucosal bacterial samples of HCT patients. DGGE profiles of amplified 16S rDNA from mucosal bacterial samples of HCT patients are shown. a Quinolone prophylaxis and/or β- lactam monotherapy group. b β- Lactam-glycopeptide combination therapy group. bfr, before HCT sample; aft, after HCT sample. Intense bands (a a– ao, b a–z) were sequenced, and bacterial species identified from the sequence data are shown in Table 2. M, marker

Article Snippet: For DGGE analyses, the amplicons and DGGE marker I (Nippon Gene, Tokyo, Japan) were electrophoresed using a D-code DGGE complete system (Bio-Rad, Hercules, CA) operated at 60 °C for 12 h at 100 V in a linear 25–65% denaturing agent gradient (100% denaturing agent consisted of 7mol/L urea and 40% deionized formamide) with 8% polyacrylamide gels (polyacrylamide gel, ratio of acrylamide HG (Wako Pure Chemical Industries, Osaka, Japan) to bisacrylamide (Wako Pure Chemical Industries), 37.5:1).

Techniques: Amplification, Glycoproteomics, Sequencing, Marker