Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Peptidyl arginine deiminase immunization induces anticitrullinated protein antibodies in mice with particular MHC types

doi: 10.1073/pnas.1713112114

Figure Lengend Snippet: IgG responses to PADs in DBA/2 mice. Plates were coated with PADs and blocked with BSA. Sera from primed mice were obtained at 15, 35, 55, and 65 d postimmunization and were diluted at 1/40. ( A ) For PBS-immunized mice, nine sera were tested for murine PAD2 and human PAD4. ( B ) For PAD-immunized mice, each serum was tested against the same PAD used for each immunization. After washing, peroxidase-conjugated antimurine IgG was added. The OD was read at 405 nm. The background OD was obtained by adding each serum to a well without PAD (negative). Positive sera were defined as an OD value higher than twice the background OD.

Article Snippet: C3H/HeNHsd and DBA/2 mice were purchased from Envigo Laboratories.

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Peptidyl arginine deiminase immunization induces anticitrullinated protein antibodies in mice with particular MHC types

doi: 10.1073/pnas.1713112114

Figure Lengend Snippet: T cell responses to PADs in DBA/2 mice. Spleen and lymph nodes were obtained at 65 d postimmunization. Cells were extracted and cultured at 5 × 10 6 cells per well with 2 μg of proteins or PHA. T cell responses were evaluated by bromodeoxyruridine incorporation. Positive responses were defined as an OD value higher than twice the OD for cells cultured without protein.

Article Snippet: C3H/HeNHsd and DBA/2 mice were purchased from Envigo Laboratories.

Techniques: Cell Culture

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Peptidyl arginine deiminase immunization induces anticitrullinated protein antibodies in mice with particular MHC types

doi: 10.1073/pnas.1713112114

Figure Lengend Snippet: Citrullinated peptide detection in the sera of DBA/2 mice immunized with PADs or PBS. ELISA plates were coated with 46 citrullinated peptides and blocked with BSA. Sera from primed mice obtained at 55 and 65 d postimmunization were diluted at 1/80. After washing, peroxidase-conjugated antimurine IgG was added. The OD was read at 405 nm. The background OD was obtained by adding each serum to a well without peptide. Positive sera were defined as an OD value higher than twice the background OD. A column corresponded to one mouse.

Article Snippet: C3H/HeNHsd and DBA/2 mice were purchased from Envigo Laboratories.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia

Article Title: Selected 68 Ga-siderophores versus 68 Ga-colloid and 68 Ga-citrate: biodistribution and small animal imaging in mice

doi: 10.5507/bp.2014.052

Figure Lengend Snippet: Biodistribution of 68 Ga-TAFC, 68 Ga-FOXE, 68 Ga-acetate/colloid, 68 Ga-colloid and 68 Ga-citrate in normal DBA/2 mice 30 min and 90 min p.i.

Article Snippet: The studies were performed using DBA/2 mice (Anlab, Prague, Czech Republic).

Techniques: