Journal: BBA Clinical

Article Title: Mutation of EMG1 causing Bowen–Conradi syndrome results in reduced cell proliferation rates concomitant with G2/M arrest and 18S rRNA processing delay

doi: 10.1016/j.bbacli.2014.05.002

Figure Lengend Snippet: Ribosomal subunit levels at steady-state are normal in BCS patient cells. (A, B) Sucrose gradient separation of 40S and 60S ribosomal subunits in lymphoblasts (A) and fibroblasts (B). Ribosomal subunits were detected following sucrose gradient separation by absorbance at 254 nm, and examples of the resultant tracings are shown. (C, D) Fractions were collected at regular intervals and probed by immunoblot analysis for ribosomal proteins using anti-RPS19 and anti-RPL7 antibodies to verify the separation of the small (RPS) and large (RPL) subunits. (E, F) The area under the curve was determined for individual peaks, and the ratio of large to small subunit was calculated. No significant difference between unaffected control and BCS-affected cells was found in lymphoblasts (E) and fibroblasts (F). The mean of six individual experiments and SEM are shown.

Article Snippet: Primary antibody dilutions were used as follows: RPL7 rabbit polyclonal at 1:5000 (Bethyl laboratories), RPS19 rabbit polyclonal at 1:3000 (Proteintech Group), TP53 2B2.68 mouse monoclonal at 1:2000 (Santa Cruz), p21/WAF1/Cip1 mouse monoclonal at 1:250 (Millipore), and β-actin mouse monoclonal at 1:5000 (Sigma).

Techniques: Western Blot, Control

Journal: Neural Development

Article Title: Axon fasciculation in the developing olfactory nerve

doi: 10.1186/1749-8104-5-20

Figure Lengend Snippet: Primary antibodies

Article Snippet: DBA (Biotinylated) , Vector Laboratories , NA , 1:50.

Techniques: Plasmid Preparation