Journal: Journal of Ginseng Research
Article Title: An Edible Gintonin Preparation from Ginseng
Figure Lengend Snippet: GC-MS spectral analysis of lipid components of edible gintonin prepared from ginseng root. Acid hydrolyzed gintonin were partitioned between distilled water and n -butanol. The n -butanol layer, after concentration, was further partitioned between distilled water and n -hexane. The n -hexane layer was subjected to gas chromatography-mass spectrometry with a DB5-MS capillary column. Several major peaks were present in hexane fraction of gintonin and were identified. The 20.94 peak was nonanedioic acid that is known to function to defense after infection in plants. The 22.04 peak of palmitic acid (C16:0) and 23.5 peak of linoleic acid (C18:2) were present as dominant fatty acids or their ester forms.
Article Snippet: The n -hexane layer was prepared for lipid and hydrophobic moiety analysis by a 6890N GC-MS system (Agilent, Santa Rosa, CA, USA) with a DB5-MS capillary column (30 cm×250 μm×0.25 μm) at the Korea Basic Science Institute (Seoul, Korea) and by gas chromatography using an Agilent 6890N chromatograph equipped with flame ionization detector and a split injection system and fitted with a supelco SPB-1 capillary column (15 m×0.32 mm inside diameter, 0.25 mm thickness) .
Techniques: Gas Chromatography-Mass Spectrometry, Concentration Assay, Gas Chromatography, Mass Spectrometry, Infection