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Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.
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Image Search Results


Journal: Frontiers in Microbiology

Article Title: Portable nanopore-sequencing technology: Trends in development and applications

doi: 10.3389/fmicb.2023.1043967

Figure Lengend Snippet:

Article Snippet: BulkVis , An app to visualize raw squiggle data from Oxford Nanopore Technologies (ONT) bulk files , https://github.com/LooseLab/bulkVis.

Techniques: Sequencing, Variant Assay, Selection, Software

Journal: Frontiers in Microbiology

Article Title: Portable nanopore-sequencing technology: Trends in development and applications

doi: 10.3389/fmicb.2023.1043967

Figure Lengend Snippet:

Article Snippet: BulkVis , An app to visualize raw squiggle data from Oxford Nanopore Technologies (ONT) bulk files , https://github.com/LooseLab/bulkVis.

Techniques: Sequencing, Sampling, Illumina Sequencing, Scaffolding, Software, Generated

Journal: Frontiers in Microbiology

Article Title: Portable nanopore-sequencing technology: Trends in development and applications

doi: 10.3389/fmicb.2023.1043967

Figure Lengend Snippet:

Article Snippet: BulkVis , An app to visualize raw squiggle data from Oxford Nanopore Technologies (ONT) bulk files , https://github.com/LooseLab/bulkVis.

Techniques: Nanopore Sequencing, Sequencing, Methylation, Modification, Generated, Extraction

Journal: Frontiers in Microbiology

Article Title: Portable nanopore-sequencing technology: Trends in development and applications

doi: 10.3389/fmicb.2023.1043967

Figure Lengend Snippet:

Article Snippet: BulkVis , An app to visualize raw squiggle data from Oxford Nanopore Technologies (ONT) bulk files , https://github.com/LooseLab/bulkVis.

Techniques: DNA Methylation Assay, Nanopore Sequencing, Modification, Control, RNA Sequencing, Sequencing, Methylation, Bacteria

Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.

Journal: Toxicological Sciences

Article Title: Nonionic Ethoxylated Surfactants Induce Adipogenesis in 3T3-L1 Cells

doi: 10.1093/toxsci/kfx234

Figure Lengend Snippet: Ethoxylated Surfactants May Promote Adipogenesis via Inhibition of TRβ. Antagonist activity for the TRβ as measured via FRET reporter gene assay using the TRβ GeneBLAzer Reporter Assay as described in the Methods. Percent agonist TRβ activity for the T3 positive control relative to its maximum response (A), percent TRβ antagonism of intra-assay EC80 T3 (concentration that exhibits 80% of maximum T3 response; 0.3 nM) for various alkyl chain length ethoxylated surfactants (B), inhibition or enhancement of cell viability relative to vehicle control for various alkyl chain length ethoxylated surfactants (C), percent TRβ antagonism relative to intra-assay EC80 T3 for various ethoxylate chain length NPEOs (D), and inhibition or enhancement of cell viability relative to vehicle control for various ethoxylate chain length NPEOs (E). Data presented as mean ± SEM from 3 independent experiments. * indicates lowest concentration with significant antagonist activity relative to EC80 T3, p < .05, as per 1-way ANOVA and Dunnett’s posthoc test in GraphPad Prism 7.0.

Article Snippet: EC20/50 values were estimated using curves generated from raw fluorescence data using a 4-parameter variable-slope Hill model in GraphPad Prism 7.0.

Techniques: Inhibition, Activity Assay, Reporter Gene Assay, Reporter Assay, Positive Control, Intra Assay, Concentration Assay