Journal: Nature neuroscience

Article Title: Globally reduced N 6 -methyladenosine (m 6 A) in C9ORF72-ALS/FTD dysregulates RNA metabolism and contributes to neurodegeneration

doi: 10.1038/s41593-023-01374-9

Figure Lengend Snippet: a, Proteomic heatmap (left) and boxplots (right) of the m6A “writer” complex core components in the iPSNs of control (n=26) and C9ORF72-ALS/FTD patients (n=22). Data were analyzed using the proteomic database from Answer ALS and NeuroLINC center. Batch controls were used for normalization of the AnswerALS data. Box plots indicate the interquartile range with the central line representing the median, and the vertical lines extend to the extreme values in the group. b,c, METTL3(b) and (c) METTL14 RNA expression levels were measured by RT-qPCR in control and C9 iPSC, iPSN and lymphoblast cells. Points represent individual cell lines, including one pair with isogenic control in which the repeat expansion was removed71. The isogenic control iPSC was set to 1. n=6 for both control and patient iPSC lines. n=6 for control and 5 for C9ORF72-ALS lymphoblast lines. Date are mean ± s.e.m. METTL3: iPSC, p=0.1042; iPSN, ***p=0.0006; lympho, p=0.8356. METTL14: iPSC, p=0.2328; iPSN, ***p=0.0006; lympho, p=0.0944. Two-tailed t test. d, UHPLC-QQQ-MS/MS quantification of the m6A/A ratio in mRNAs from iPSNs. The mRNAs of the isogenic pair (circle-cross) were extracted by poly-A selection; the mRNAs of the other iPSN lines were extracted by ribosomal RNA depletion. Two-tailed t test was performed on 5 pairs of control and C9 lines (ribosomal RNA depletion samples). Date are mean ± s.e.m. ****p<0.0001 by two-tailed t test. e, UHPLC-QQQ-MS/MS quantification of the m6A/A ratio in mRNAs from postmortem brain tissues extracted by poly-A selection. Points represent individual patient samples. n=6 for both control and patient samples. Date are mean ± s.e.m. *p=0.0168, **p=0.0039, ****p<0.0001, by two-tailed t test.

Article Snippet: Proteomic data analysis Proteomic data matrix was downloaded from NeuroLINC and Answer ALS databases (see Supplementary Table 6 for demographics).

Techniques: Control, RNA Expression, Quantitative RT-PCR, Two Tailed Test, Tandem Mass Spectroscopy, Selection

Journal: Nature neuroscience

Article Title: Globally reduced N 6 -methyladenosine (m 6 A) in C9ORF72-ALS/FTD dysregulates RNA metabolism and contributes to neurodegeneration

doi: 10.1038/s41593-023-01374-9

Figure Lengend Snippet: a, Proteomic heatmap of the m6A pathway components in control (n=26) and C9ORF72-ALS/FTD (n=22) iPSNs. Data were normalized to the batch controls. b, Boxplots of the proteomic data in (a). Box plots indicate the interquartile range with the central line representing the median, and the vertical lines extend to the extreme values in the group. c, Western blotting (left) and quantification (right) of METTL3 and METTL14 expression in control and C9ORF72 iPSNs. Data are mean ± s.e.m. ***p=0.0008 and 0.0003, respectively, by two-tailed t test. d, Relative RNA expression of neuronal marker genes (MAP2, PSD95 and TUBB3) was measured by RT-qPCR in control and C9 iPSNs, showing comparable neuron maturity between control and C9. Points represent individual control or patient lines. n=6 in each group. Data are mean ± s.e.m. MAP2: p=0.304; PSD95: p=0.86; TUBB3: p=0.716, by two-tailed t test.

Article Snippet: Proteomic data analysis Proteomic data matrix was downloaded from NeuroLINC and Answer ALS databases (see Supplementary Table 6 for demographics).

Techniques: Gene Expression, Control, Western Blot, Expressing, Two Tailed Test, RNA Expression, Marker, Quantitative RT-PCR

Journal: Nature neuroscience

Article Title: Globally reduced N 6 -methyladenosine (m 6 A) in C9ORF72-ALS/FTD dysregulates RNA metabolism and contributes to neurodegeneration

doi: 10.1038/s41593-023-01374-9

Figure Lengend Snippet: a, Proteomic heatmap of the core components of m6A “writer” complex in control (n=19) and sporadic ALS (n=93) iPSNs. Data were normalized to the batch controls. b, Boxplots of the proteomic data in (a). Box plots indicate the interquartile range with the central line representing the median, and the vertical lines extend to the extreme values in the group. c, UHPLC-QQQ-MS/MS quantification of the m6A/A ratio in poly-A mRNAs from control (n=5) and sporadic ALS patient (n=7) postmortem frontal cortex. Data are mean ± s.e.m. Points represent individual samples. *p=0.0247 by two-tailed t test.

Article Snippet: Proteomic data analysis Proteomic data matrix was downloaded from NeuroLINC and Answer ALS databases (see Supplementary Table 6 for demographics).

Techniques: Expressing, Control, Tandem Mass Spectroscopy, Two Tailed Test

Journal: Vaccine: X

Article Title: Vaccine traceability: Key learnings from the supply chain initiative by manufacturers from emerging countries

doi: 10.1016/j.jvacx.2023.100366

Figure Lengend Snippet: Summary of six pilot studies focused on primary and secondary vaccine packaging.

Article Snippet: During another Supply Chain Working Group meeting, held in November 2019, PT BioFarma presented its work on vaccines traceability, which was documented and inspired a project to accelerate the implementation of GS1 2D data matrix barcodes on secondary or primary packaging, through supporting pilot studies, coordinated through a purposely created Traceability Pilot Study Consortium .

Techniques: Labeling, Software

Journal: Vaccine: X

Article Title: Vaccine traceability: Key learnings from the supply chain initiative by manufacturers from emerging countries

doi: 10.1016/j.jvacx.2023.100366

Figure Lengend Snippet: Success-enabling factors for implementation of vaccine traceability tools.

Article Snippet: During another Supply Chain Working Group meeting, held in November 2019, PT BioFarma presented its work on vaccines traceability, which was documented and inspired a project to accelerate the implementation of GS1 2D data matrix barcodes on secondary or primary packaging, through supporting pilot studies, coordinated through a purposely created Traceability Pilot Study Consortium .

Techniques: