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Image Search Results
Journal: PLoS ONE
Article Title: The Extracytoplasmic Stress Factor, σ E , Is Required to Maintain Cell Envelope Integrity in Escherichia coli
doi: 10.1371/journal.pone.0001573
Figure Lengend Snippet: Cultures of SEA007 and SEA008 were grown as in . Samples were taken approximately 2.5 hrs. after induction of rseA and rseB overexpression. (A–D) Images of live cells are shown using differential interference microscopy (DIC, column 1) and fluorescence microscopy following addition of FM4-64 to visualize membranes (red, column 2), DAPI to visualize DNA (blue, column 3), and expression of YFP to visualize the cytoplasm (green, column 4). The three fluorescent micrographs are overlaid in column 5. (A) Images of the SEA008 control strain in which σ E was not inhibited are shown. (B–D) Images of SEA007 following σ E inhibition, reveal blebs that contain YFP and stain with DAPI (B and C) and that contain YFP but do not stain with DAPI (D). In (D), the arrow marks a bleb lacking DAPI staining and the arrowhead marks a lysed cell that retained DAPI staining, but lost YFP. Scale bars are 2 µm. Over 1,000 cells were examined by fluorescence microscopy and typical micrographs are represented here. Scanning electron micrographs (E) and transmission electron micrographs (F) of SEA007 following σ E inhibition. Scale bars are 1 µm. No blebs were seen on cells in control cultures.
Article Snippet: Cells were mounted on freshly prepared polylysine coated coverslips and visualized using an Olympus BX-61 epi-fluorescent microscope with a 100× phase oil objective (NA of 1.3), using an Olympus 41028 filter cube for YFP, Olympus 31000v2 filter cube for
Techniques: Over Expression, Microscopy, Fluorescence, Expressing, Inhibition, Staining, Transmission Assay
Journal: The Journal of Neuroscience
Article Title: Cholinergic Control of GnRH Neuron Physiology and Luteinizing Hormone Secretion in Male Mice: Involvement of ACh/GABA Cotransmission
doi: 10.1523/JNEUROSCI.1780-23.2024
Figure Lengend Snippet: An overview of the reagents used for immunohistochemistry
Article Snippet: At the same time, to detect Cy5 emission, a He–Ne laser (637 nm) and a
Techniques: Immuno-Electron Microscopy, Electron Microscopy