Journal: Journal of Bacteriology

Article Title: Genetic Evidence for the Involvement of the S-Layer Protein Gene sap and the Sporulation Genes spo0A , spo0B , and spo0F in Phage AP50c Infection of Bacillus anthracis

doi: 10.1128/JB.00739-13

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: It is temperature sensitive for replication and contains a chloramphenicol resistance gene for Gram-positive organisms, as well as a gene encoding the C9 variant of the Himar1 transposase and an erythromycin resistance gene, located between two inverted repeats that are transposed by the transposase. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid a Description Source or reference Strains Escherichia coli GM119 dam dcm mutant 36 C2925 dam dcm mutant New England Biolabs B. anthracis 34F 2 Sterne/pXO1 + /pXO2 − P. C. Hanna lab 7702 Sterne/pXO1 + /pXO2 − T. M. Koehler lab JB220 7702 ΔBAS2245 This study BAP350 7702 Δ csaB This study BA749 7702 ΔBAS0566 This study BA750 7702 Δ sap This study BA751 7702 Δ eag This study BA752 7702 ΔBAS1792 This study BA753 7702 ΔBAS3559 This study BA754 7702 Δ spo0A This study BA755 7702 Δ spo0F This study DP-B-5747 JB220 Δ spo0B This study B. cereus RS110 ATCC 4342 60 RS112 RTS100 ( B. anthracis -like) RS415 ATCC 25621 61 RS416 ATCC 43881 62 RS417 ATCC 14893 63 RS431 ATCC 27877 64 RS432 ATCC 7064 (NRS201) 60 RS436 B. cereus transition state strain CDC13100 47 RS437 B. cereus transition state strain CDC13140 47 RS438 B. cereus transition state strain CDC32805 47 RS710 B. cereus var.

Techniques: Plasmid Preparation, Mutagenesis, Expressing