Journal: Bioactive Materials

Article Title: Upconversion nanoparticles regulated drug & gas dual-effective nanoplatform for the targeting cooperated therapy of thrombus and anticoagulation

doi: 10.1016/j.bioactmat.2022.03.013

Figure Lengend Snippet: NIR responsive characteristics of UCATS. A) UCL spectra of UCM, UCA and UCATS. B) UV–vis absorption spectra changes of UCA before and after irradiation with 808 nm NIR light (2 W cm −2 ). C) UV–vis absorption spectra changes of SNAP before and after irradiation by vis (room light) and UV (365 nm) light for different time. D) NIR-triggered controllable release of UK from UCATS-UK. E) NIR-triggered NO release from UCATS at different concentrations and SNAP. 808 nm NIR: 2 W cm −2 . F) On/Off behavior of NO release triggered by 808 nm NIR light (2 W cm −2 ). G) The fluorescent images of HUVECs stained with NO fluorescent probe (DAF-FM DA) and Hoechst 33342 after being treated with UCATS under dark conditions or 808 nm NIR irradiation (1 W cm −2 ). Scale bar = 50 μm. Data are means ± s.d. (n = 3).

Article Snippet: Ammonium fluoride (NH 4 F, 98.0%) and tetraethyl orthosilicate (TEOS, 99.0%) were purchased from Alfa Co., LTD. DAF-FM DA and Hoechst 33342 were purchased from MCE Co., LTD. 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM, 97.0%) was purchased from J&K Scientific.

Techniques: Irradiation, Staining

Journal: Frontiers in Plant Science

Article Title: Reactive Oxygen Species (ROS) Generation Is Indispensable for Haustorium Formation of the Root Parasitic Plant Striga hermonthica

doi: 10.3389/fpls.2019.00328

Figure Lengend Snippet: Effects of external application of ROS-scavenging enzymes on haustorium formation. S. hermonthica seedlings were exposed to DMBQ or syringic acid with the ROS-scavenging enzymes, catalase (A) or SOD (B) . Ten micrometer of DMBQ (A,B) and 100 μM (A) or 10 μM (B) of syringic acid were used in each experiment to allow comparison with a previous report on catalase (Keyes et al., ). After 24 h exposure, haustorium formation rates were evaluated. Experiments were performed at least twice independently with similar results. Red triangles and blue squares indicate treatment with DMBQ and syringic acid, respectively, and error bars indicate standard error (SE: n = 3). ∞ indicates without enzyme control. Asterisks indicate statistically significant differences between samples with and without the indicated enzymes as detected by a Student's t -test (* P < 0.05, red asterisk, DMBQ; blue asterisk, syringic acid).

Article Snippet: For visualization of NO − and · OH, Striga haustoria induced by 10 μM DMBQ or syringic acid for 24 h were stained with 10 μM diaminoflurescein-2-diacetate (DAF-2 DA, Goryo Chemical, Inc., Sapporo, Japan) or 10 μM aminophenyl fluorescein (APF, Goryo Chemical, Inc.), respectively, for 30 min, and were then washed with sterile water a few times.

Techniques: Comparison, Control

Journal: Frontiers in Plant Science

Article Title: Reactive Oxygen Species (ROS) Generation Is Indispensable for Haustorium Formation of the Root Parasitic Plant Striga hermonthica

doi: 10.3389/fpls.2019.00328

Figure Lengend Snippet: Visualization of H 2 O 2 accumulation in haustoria of S. hermonthica. S. hermonthica seedlings were untreated (A) or treated with 10 μM DMBQ (B) or 10 μM syringic acid (C) for 24 h and accumulation of H 2 O 2 was visualized by carboxy-H2DFFDA staining. Fluorescence images (left panels), DIC images (middle panels), and overlay images (right panels) are shown. Scale bar indicates 500 μm. The experiments were repeated at least three times and representative images are shown.

Article Snippet: For visualization of NO − and · OH, Striga haustoria induced by 10 μM DMBQ or syringic acid for 24 h were stained with 10 μM diaminoflurescein-2-diacetate (DAF-2 DA, Goryo Chemical, Inc., Sapporo, Japan) or 10 μM aminophenyl fluorescein (APF, Goryo Chemical, Inc.), respectively, for 30 min, and were then washed with sterile water a few times.

Techniques: Staining, Fluorescence

Journal: Frontiers in Plant Science

Article Title: Reactive Oxygen Species (ROS) Generation Is Indispensable for Haustorium Formation of the Root Parasitic Plant Striga hermonthica

doi: 10.3389/fpls.2019.00328

Figure Lengend Snippet: Visualization of O 2 − accumulation in haustoria of S. hermonthica. S. hermonthica seedlings were untreated (A) or treated with 10 μM DMBQ (B) or 10 μM syringic acid (C) for 24 h and stained with NBT to visualize O 2 − . NBT-stained seedlings (left panels) and no-staining control (right panels) are shown. Scale bar indicates 500 μm. The experiments were repeated at least three times and representative images are shown.

Article Snippet: For visualization of NO − and · OH, Striga haustoria induced by 10 μM DMBQ or syringic acid for 24 h were stained with 10 μM diaminoflurescein-2-diacetate (DAF-2 DA, Goryo Chemical, Inc., Sapporo, Japan) or 10 μM aminophenyl fluorescein (APF, Goryo Chemical, Inc.), respectively, for 30 min, and were then washed with sterile water a few times.

Techniques: Staining, Control

Journal: Frontiers in Plant Science

Article Title: Reactive Oxygen Species (ROS) Generation Is Indispensable for Haustorium Formation of the Root Parasitic Plant Striga hermonthica

doi: 10.3389/fpls.2019.00328

Figure Lengend Snippet: Effects of ROS or ROS-regulating enzyme inhibitors on haustorium formation. Haustorium formation rates were calculated after 24 h treatment with 10 μM (A) , 100 μM (B) , 1 mM (C) of the inhibitors indicated, in the presence of 10 μM DMBQ or syringic acid. Error bars indicate SE ( n = 3), and NT, not tested. Different lower-case letters represent significant differences as determined by a Tukey HSD test ( p < 0.05).

Article Snippet: For visualization of NO − and · OH, Striga haustoria induced by 10 μM DMBQ or syringic acid for 24 h were stained with 10 μM diaminoflurescein-2-diacetate (DAF-2 DA, Goryo Chemical, Inc., Sapporo, Japan) or 10 μM aminophenyl fluorescein (APF, Goryo Chemical, Inc.), respectively, for 30 min, and were then washed with sterile water a few times.

Techniques:

Journal: Frontiers in Plant Science

Article Title: Reactive Oxygen Species (ROS) Generation Is Indispensable for Haustorium Formation of the Root Parasitic Plant Striga hermonthica

doi: 10.3389/fpls.2019.00328

Figure Lengend Snippet: Effects of Kl and DDC on haustorium formation. (A) Effect of 5 mM KI on haustorium induction by DMBQ or syringic acid. (B) Concentration-dependent inhibition of DDC on haustorium formation induced by DMBQ or syringic acid. Error bars indicate SE ( n = 3), and NT, not tested. Different lower-case letters represent significant differences as determined by a Tukey HSD test ( p < 0.05).

Article Snippet: For visualization of NO − and · OH, Striga haustoria induced by 10 μM DMBQ or syringic acid for 24 h were stained with 10 μM diaminoflurescein-2-diacetate (DAF-2 DA, Goryo Chemical, Inc., Sapporo, Japan) or 10 μM aminophenyl fluorescein (APF, Goryo Chemical, Inc.), respectively, for 30 min, and were then washed with sterile water a few times.

Techniques: Concentration Assay, Inhibition

Journal: Frontiers in Plant Science

Article Title: Reactive Oxygen Species (ROS) Generation Is Indispensable for Haustorium Formation of the Root Parasitic Plant Striga hermonthica

doi: 10.3389/fpls.2019.00328

Figure Lengend Snippet: Effects of DPI and external H 2 O 2 on haustorium formation. (A) Haustorium formation rates upon treatment with lower concentrations of DPI in the presence of 10 μM DMBQ or syringic acid. (B) Effects of external H 2 O 2 on haustorium formation rates treated with 1 μM DPI in the presence of 10 μM DMBQ or syringic acid. Error bars indicate SE ( n = 3). NT, not tested. Different lower-case letters represent significant differences as determined by a Tukey HSD test ( p < 0.05).

Article Snippet: For visualization of NO − and · OH, Striga haustoria induced by 10 μM DMBQ or syringic acid for 24 h were stained with 10 μM diaminoflurescein-2-diacetate (DAF-2 DA, Goryo Chemical, Inc., Sapporo, Japan) or 10 μM aminophenyl fluorescein (APF, Goryo Chemical, Inc.), respectively, for 30 min, and were then washed with sterile water a few times.

Techniques:

Journal: Frontiers in Plant Science

Article Title: Reactive Oxygen Species (ROS) Generation Is Indispensable for Haustorium Formation of the Root Parasitic Plant Striga hermonthica

doi: 10.3389/fpls.2019.00328

Figure Lengend Snippet: Effects of external peroxidase on haustorium formation by DMBQ and syringic acid. (A) Concentration-dependent haustorium formation rates of DMBQ and syringic acid treatments. S. hermonthica seedlings were exposed to 0.05, 0.1, 1, and 10 μM of DMBQ or syringic acid, and haustorium formation rates were calculated after 24 h. (B,C) Striga seedlings were exposed to a combination of 0.01 U/μl HRP and 0.001% H 2 O 2 in the presence of various concentrations of syringic acid (B) or DMBQ (C) and haustorium formation rates were calculated after 24 h. (D) S. hermonthica seedlings were exposed to a high concentration of HRP at 0.1 U/μl and 0.001% H 2 O 2 , and haustorium formation rates were calculated after 24 h. Error bars indicate SE ( n = 3). NT, not tested. Different lower-case letters represent significant differences as detected by a Tukey HSD test ( p < 0.05).

Article Snippet: For visualization of NO − and · OH, Striga haustoria induced by 10 μM DMBQ or syringic acid for 24 h were stained with 10 μM diaminoflurescein-2-diacetate (DAF-2 DA, Goryo Chemical, Inc., Sapporo, Japan) or 10 μM aminophenyl fluorescein (APF, Goryo Chemical, Inc.), respectively, for 30 min, and were then washed with sterile water a few times.

Techniques: Concentration Assay

Journal: Frontiers in Plant Science

Article Title: Reactive Oxygen Species (ROS) Generation Is Indispensable for Haustorium Formation of the Root Parasitic Plant Striga hermonthica

doi: 10.3389/fpls.2019.00328

Figure Lengend Snippet: Models for haustorium induction in S. hermonthica . (A) A model from previous studies [modified from (Keyes et al., )]. DMBQ is produced from syringic acid by Striga -derived peroxidase and H 2 O 2 . DMBQ acts as a signal for Striga seedlings to form a haustorium. (B) A model suggested by this study. Striga -derived peroxidase can convert syringic acid to DMBQ, which may be further converted to a compound with higher haustorium-inducing activity. In addition, peroxidase enhances haustorium formation independently from HIFs. NADPH oxidase and H 2 O 2 production have a pivotal role in haustorium formation in Striga .

Article Snippet: For visualization of NO − and · OH, Striga haustoria induced by 10 μM DMBQ or syringic acid for 24 h were stained with 10 μM diaminoflurescein-2-diacetate (DAF-2 DA, Goryo Chemical, Inc., Sapporo, Japan) or 10 μM aminophenyl fluorescein (APF, Goryo Chemical, Inc.), respectively, for 30 min, and were then washed with sterile water a few times.

Techniques: Modification, Produced, Derivative Assay, Activity Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Epidermal growth factor receptor targeting IgG3 triggers complement-mediated lysis of decay-accelerating factor expressing tumor cells through the alternative pathway amplification loop.

doi: 10.4049/jimmunol.1400329

Figure Lengend Snippet: FIGURE 4. CML triggered by anti–EGFR-IgG3 negatively correlates with CD55 and CD59 expression levels. (A) Surface expression levels of CD46, CD55, and CD59 on analyzed cell lines were quantified by calibrated flow cytometry. Means 6 SEM of at least three independent experiments are presented. (B) Correlations between CD46, CD55, or CD59 and anti–EGFR-IgG3–mediated CDC were calculated for all four cell lines. CDC results at 2 mg/ml Ab concentration were taken from experiments presented in Fig. 2. (C) A431 cells were seeded into 10-cm plates and grown overnight. On the following day, cells were transfected with 50 nM control siRNA or with single siRNAs specific for CD46, CD55, CD59, or with a combination of all three mCRP-specific siRNAs for 72 h. Efficiency of siRNA-induced knockdown was analyzed by direct flow cytometry using fluorochrome-labeled, mCRP- specific Abs (CD46-Pacific blue, CD55-PE, CD59-FITC), or respective control Abs. (D–G) CDC against control siRNA or mCRP-specific, siRNA- transfected A431 cells was analyzed by 3-h [51Cr] release assays in the presence of 25% v/v human serum and anti–EGFR-IgG1 (upper panels), anti– EGFR-IgG3 (lower panels), as well as the respective control Abs at increasing concentrations. (H) Concentration-dependent binding of CD55-Ab (BRIC216, mouse IgG1) to A431 cells was analyzed by indirect immunofluorescence. Results from one representative experiment are presented. (I) CDC triggered by anti–EGFR-IgG1, anti–EGFR-IgG3, or respective control Abs (all at 66.67 nM) against A431 cells in the presence of saturating concentrations of CD55-Ab or a control Ab (both at 66.67 nM) was analyzed by 3-h [51Cr] release assays in the presence of 25% v/v human (Figure legend continues)

Article Snippet: To block complement regulatory activity of CD55, we used mouse anti-human CD55 (66.67 nM, BRIC216, mouse IgG1; Bio-Rad) blocking mAb in CDC experiments at saturating concentrations.

Techniques: Expressing, Cytometry, Concentration Assay, Transfection, Control, Knockdown, Labeling, Binding Assay

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Epidermal growth factor receptor targeting IgG3 triggers complement-mediated lysis of decay-accelerating factor expressing tumor cells through the alternative pathway amplification loop.

doi: 10.4049/jimmunol.1400329

Figure Lengend Snippet: FIGURE 5. CD55 dampens anti–EGFR-IgG3–triggered CML and promotes C1q-dependent induction of AP amplification. BHK-EGFR+ #5 cells were transiently transfected with a control vector or a CD55 vector for 48 h. (A) Cell-surface expression of CD55 was analyzed by direct flow cytometry using PE-conjugated CD55-specific or control Abs. (B–D) The influence of CD55 overexpression on anti–EGFR-IgG3–mediated CDC was investigated by [51Cr] release assays either (B) in an Ab concentration–response curve, (C) in a time-dependent manner, or (D) in serum titration experiments. (E–H) The influence of the alternative complement pathway inhibitor CRIg (E and G), the presence of C1q in serum (F; at 66.67 nM Ab concentration; mean 6 SEM of triplicates), as well as of factor B (H; 13.33 nM Ab concentration, 12.5% v/v factor B–depleted serum, 200 mg/ml factor B), on anti–EGFR-IgG3–mediated CDC was analyzed using either (E and F) control vector–transfected or CD55 vector–transfected BHK-EGFR+ #5 cells or (G and H) DiFi cells (66.67 nM Ab concentration). (I) Deposition of factor Bb on control vector– or CD55 vector–transfected BHK-EGFR+ #5 cells was analyzed by flow cytometry. Relative deposition levels were calculated by equating RFI measured in the absence of Ab with 100%. Results are presented as mean 6 SEM of at least three independent experiments with different blood donors. *p # 0.05 anti–EGFR-IgG3 versus respective control Ab; (B–D, I) #p # 0.05 control vector versus CD55 vector; (E and G) #p # 0.05 without CRIg-Fc versus CRIg-Fc; (H) #p # 0.05 w/o factor B versus with factor B.

Article Snippet: To block complement regulatory activity of CD55, we used mouse anti-human CD55 (66.67 nM, BRIC216, mouse IgG1; Bio-Rad) blocking mAb in CDC experiments at saturating concentrations.

Techniques: Transfection, Control, Plasmid Preparation, Expressing, Cytometry, Over Expression, Concentration Assay, Titration

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Epidermal growth factor receptor targeting IgG3 triggers complement-mediated lysis of decay-accelerating factor expressing tumor cells through the alternative pathway amplification loop.

doi: 10.4049/jimmunol.1400329

Figure Lengend Snippet: FIGURE 6. Overview of complement activation by human anti–EGFR-IgG3 in the context of CD55 expression. On CD55-deficient target cells (left panel), anti–EGFR-IgG3 mediates strong C3b but low C4b deposition and induces assembly of classical and alternative C3 convertases, predominantly resulting in the induction of fast and efficient CDC via the classical pathway of complement activation. In contrast, on CD55-expressing target cells (right panel), CD55 mainly accelerates the decay of low amounts of classical C3 convertases, leading to amplification of the AP and finally to slow and inefficient CDC induction.

Article Snippet: To block complement regulatory activity of CD55, we used mouse anti-human CD55 (66.67 nM, BRIC216, mouse IgG1; Bio-Rad) blocking mAb in CDC experiments at saturating concentrations.

Techniques: Activation Assay, Expressing

Journal: Clinical and experimental immunology

Article Title: miR-132-3p regulates antibody-mediated complement-dependent cytotoxicity in colon cancer cells by directly targeting CD55.

doi: 10.1093/cei/uxac120

Figure Lengend Snippet: Figure 1: CD55 is a direct target of miR-132-3p. (A) The relative expression level of miRNAs in the Ls-174T cell line was detected by RT-qPCR and compared to the miRNAs levels in the LoVo cell line. (B) miR-132-3p expression level in LoVo or Ls-174T cell lines transfected with the miR-132-3p mimics or inhibitor and their respective negative controls. CD55 mRNA (C) and protein (D) expression in the LoVo or Ls-174T cell line transfected with the miR-132-3p mimics or inhibitor and their respective negative controls. (E) Schematic representation of the CD55-3ʹ-UTR (untranslated region) reporter construct and sequence alignment between miR-132-3p and the CD55 3ʹ-UTR wild-type and CD55 3ʹ-UTR mutant sequences. Mutations were generated based on the miR-132-3p-binding sequence of the CD55 3ʹ-UTR as indicated. Dual-luciferase reporter data with the wild-type and mutated 3ʹ-UTR of CD55 are shown after miR-132-3p overexpression. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The membranes were blocked with 5% skim milk in TBS containing 0.05% Tween 20 at room temperature for 1 h. Membranes were incubated overnight with primary antibodies against CD55 (1:400, ABclonal, Woburn, MA, USA) and β-actin (1:8000, ZenBioScience, Chengdu, China) in 5% non-fat milk at 4°C.

Techniques: Expressing, Quantitative RT-PCR, Transfection, Construct, Sequencing, Mutagenesis, Generated, Binding Assay, Luciferase, Over Expression

Journal: Clinical and experimental immunology

Article Title: miR-132-3p regulates antibody-mediated complement-dependent cytotoxicity in colon cancer cells by directly targeting CD55.

doi: 10.1093/cei/uxac120

Figure Lengend Snippet: Figure 4: miR-132-3p and CD55 expression level in colon tumour tissues and adjacent normal tissues. (A) H&E staining of frozen tissue samples (×100). (B) Representative images of CD55 expression in human normal colon tissues and colon cancer tissues, as determined through IHC. a: Normal colon tissues. b: Highly differentiated colon cancer tissues. c: Moderately differentiated colon cancer tissues. d: Poorly differentiated colon cancer tissues. (C) Expression of CD55 mRNA and miR-132-3p in paired tumour tissues and normal tissues. (D) Correlation analysis of CD55 protein based on IHC and CD55 based on RT-qPCR (left), as well as CD55 protein based on IHC and miR-132-3p based on RT-qPCR. *P < 0.05, ***P < 0.001.

Article Snippet: The membranes were blocked with 5% skim milk in TBS containing 0.05% Tween 20 at room temperature for 1 h. Membranes were incubated overnight with primary antibodies against CD55 (1:400, ABclonal, Woburn, MA, USA) and β-actin (1:8000, ZenBioScience, Chengdu, China) in 5% non-fat milk at 4°C.

Techniques: Expressing, Staining, Quantitative RT-PCR

Journal: iScience

Article Title: CRISPR-Cas9 screening reveals a distinct class of MHC-I binders with precise HLA-peptide recognition

doi: 10.1016/j.isci.2024.110120

Figure Lengend Snippet: Receptor-ligand CRISPR-Cas9 activation screen reveals that CD55 interacts with HLA-C∗07:01-VRIG tetramers (A) Schematic of the receptor ligand CRISPR-Cas9 activation screen. K562 cells transduced with a genome-wide activation library were stained with a pool of three HLA tetramers (HLA-A∗02:01-NLVP, HLA-B∗07:02-TPRV, and HLA-C∗07:01-VRIG), and enriched gRNAs in stained cells were identified using NGS. (B) SigmaFC scores of genes from two replicate screens. SigmaFC scores were calculated using PinAplPy, and top hits are annotated. (C) K562 cells stably expressing dCas9 and transduced with a gRNA upregulating CD55 or a control guide were stained with the HLA-A, -B, -C, or tetramers as in (A) or with HLA-E∗01:01-VMAP tetramers and analyzed by flow cytometry. (D) In vitro co-immunoprecipitation of recombinant CD55-Fc with HLA-A∗02:01-NLVP, HLA-B∗07:02-TPRV, HLA-C∗07:01-VRIG, or HLA-E∗01:01-VMAP tetramers. (E) Three different cell lines (HeLa, PC-3M, or SiHa) that express CD55 endogenously were stained for CD55 (top) or with HLA-C∗07:01-VRIG tetramers (bottom) and analyzed by flow cytometry. (F) HeLa wild-type or HeLa CD55 KO cells were stained with αCD55 or HLA-C∗07:01-VRIG tetramers and analyzed by flow cytometry. All data except (B) represent at least three independent experiments. CRISPRa, CRISPR activation screen; TMs, tetramers; WT, wild-type; KO, knockout. Related to Figure S1 and Table S1 .

Article Snippet: Antibodies used in this study were anti-heparan sulfate chains (AMSBIO, F58-10E4), FITC anti-human CD55 (Biolegend, 311306), anti-human CD55 BRIC110 (ARP, 08-9402-2, targets SCR2 of CD55), anti-human CD55 BRIC216 (Biorad, MCA914T, targets SCR3 of CD55), anti-human CD55 MAB2009 (R&D systems, MAB2009-SP, targets SCR1 of CD55), anti-SDC2 APC (R&D systems, FAB2965A), anti-SDC4 APC (R&D systems, FAB29181A), anti-CD55 APC (Biolegend, #311311), or goat-anti-mouse APC (Biolegend, #405308).

Techniques: CRISPR, Activation Assay, Transduction, Genome Wide, Staining, Stable Transfection, Expressing, Control, Flow Cytometry, In Vitro, Immunoprecipitation, Recombinant, Knock-Out

Journal: iScience

Article Title: CRISPR-Cas9 screening reveals a distinct class of MHC-I binders with precise HLA-peptide recognition

doi: 10.1016/j.isci.2024.110120

Figure Lengend Snippet: Interaction of CD55 with HLA-C∗07:01-VRIG tetramers is allotype and peptide specific (A) HEK293T cells were transfected with a plasmid containing GFP and a truncation mutant of CD55 and analyzed by flow cytometry. GFP+ positive cells were analyzed for staining with HLA-C∗07:01-VRIG. Each mutant removes an additional SCR domain from CD55. Data are represented as mean ± SD. (B) HeLa cells were stained with HLA-C∗07:01-VRIG tetramers after pre-incubation with CD55 blocking antibodies targeting different SCR domains on CD55 and analyzed by flow cytometry. (C) HeLa cells were stained with either HLA-C∗07:01 or HLA-C∗07:02 tetramers loaded with the VRIG peptide and analyzed by flow cytometry. (D) HeLa cells were stained with HLA-C∗07:01 tetramers loaded with different alanine mutants of the VRIGHLYIL peptide and analyzed by flow cytometry. (E) CD55-Fc was immobilized on a Prot-G chip for SPR data using HLA-C∗07:01-VRIG tetramers as analyte to determine interaction on and off rates and K D . Response units were measured with increasing concentrations of HLA-C∗07:01-VRIG tetramers. All data represent at least three independent experiments, except (E), which represents a biological duplicate. FL, full length. Related to Figure S2 , Tables S2 and .

Article Snippet: Antibodies used in this study were anti-heparan sulfate chains (AMSBIO, F58-10E4), FITC anti-human CD55 (Biolegend, 311306), anti-human CD55 BRIC110 (ARP, 08-9402-2, targets SCR2 of CD55), anti-human CD55 BRIC216 (Biorad, MCA914T, targets SCR3 of CD55), anti-human CD55 MAB2009 (R&D systems, MAB2009-SP, targets SCR1 of CD55), anti-SDC2 APC (R&D systems, FAB2965A), anti-SDC4 APC (R&D systems, FAB29181A), anti-CD55 APC (Biolegend, #311311), or goat-anti-mouse APC (Biolegend, #405308).

Techniques: Transfection, Plasmid Preparation, Mutagenesis, Flow Cytometry, Staining, Incubation, Blocking Assay

Journal: iScience

Article Title: CRISPR-Cas9 screening reveals a distinct class of MHC-I binders with precise HLA-peptide recognition

doi: 10.1016/j.isci.2024.110120

Figure Lengend Snippet:

Article Snippet: Antibodies used in this study were anti-heparan sulfate chains (AMSBIO, F58-10E4), FITC anti-human CD55 (Biolegend, 311306), anti-human CD55 BRIC110 (ARP, 08-9402-2, targets SCR2 of CD55), anti-human CD55 BRIC216 (Biorad, MCA914T, targets SCR3 of CD55), anti-human CD55 MAB2009 (R&D systems, MAB2009-SP, targets SCR1 of CD55), anti-SDC2 APC (R&D systems, FAB2965A), anti-SDC4 APC (R&D systems, FAB29181A), anti-CD55 APC (Biolegend, #311311), or goat-anti-mouse APC (Biolegend, #405308).

Techniques: Virus, Recombinant, Blocking Assay, Genome Wide, Activation Assay, CRISPR, Knock-Out, Mutagenesis, Plasmid Preparation, Software, Imaging