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The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in
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Journal: Antioxidants (Basel, Switzerland)
Article Title: Human NCF1 90H Variant Promotes IL-23/IL-17-Dependent Mannan-Induced Psoriasis and Psoriatic Arthritis.
doi: 10.3390/antiox12071348
Figure Lengend Snippet: Figure 7. NCF190H variant upregulates JAK1/STAT3 pathway in mannan-induced PsA and PsO. (A) Immunohistochemical staining of p-STAT3 in the ear skin tissues. Scale bar = 200 µm. The arrows indicate the area of positive staining. (B) Statistics (n = 5). (C) Expression of JAK1/p-JAK1 and STAT3/p-STAT3 proteins in psoriatic skins after intraperitoneal injection of mannan. (D) Statistics of Western blot. Immunoblot shows one of the two experiments, with similar results (n = 3 in each experiment) and relative expression with n = 6. (E) Expression of JAK1/STAT3 proteins in psoriatic skins after epicutaneous application of mannan on the back skin of mice. (F) Statistics of western blot (n = 4). The data are shown as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; ns: no significance.
Article Snippet: For immunohistochemical (IHC) staining, skin sections (3.5 μm) were cut and stained with NCF1 (p47phox) mouse antibody (D-10) (
Techniques: Variant Assay, Immunohistochemical staining, Staining, Expressing, Injection, Western Blot