d-ap5 Search Results


d 2 amino 5 phosphonopentanoate d ap5  (Alomone Labs)
95
Alomone Labs d 2 amino 5 phosphonopentanoate d ap5
D 2 Amino 5 Phosphonopentanoate D Ap5, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d ap5  (Tocris)
96
Tocris d ap5
D Ap5, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d ap5  (MedChemExpress)
95
MedChemExpress d ap5
a , Schematic showing the spatial arrangement of two electrodes, with the left one filled with glutamate (1 mM) for activating aSMCs through puff application, while the right one records membrane currents. b , A representative trace of glutamate-induced depolarizing currents at a holding potential of –40 mV in the absence (top) and presence (bottom) of <t>D-AP5</t> and NBQX. c , The statistical analysis for b ( N = 5 cells). d , e , Still frame images of Ca 2+ spark-like ( d ) and Ca 2+ wave ( e ) in aSMCs upon 500 μM ( d ) or 1 mM ( e ) glutamate stimulation (red, high; blue, low). f , Representative trace of Ca 2+ spark-like kinetics and Ca 2+ wave of d and e . g , h , Quantification of the frequencies of Ca 2+ spark-like events and Ca 2+ waves in aSMCs treated with vehicle ( n = 4 cells), glutamate alone ( g , 500 μM; h , 1 mM; n = 5 cells for each dose), or together with D-AP5 ( n = 5 cells). These cells were collected from three independent experiments. The baseline F0 was determined by averaging 10 images without Ca2+ sparks. A Ca2+ spark was identified as a localized increase in ΔF/F0 greater than 0.2. Ca2+ waves were defined by an ΔF/F0 elevation >0.2 that propagated for more than 20 μm. i , Schematic illustration depicts the spatial configuration of electrodes, with the left one containing NMDA (100 μM) for puffing. j , Exemplary traces of NMDA-evoked currents at a holding potential of –40 mV under Mg 2+ -free artificial cerebrospinal fluid (aCSF) conditions. The first (left) and second (right) NMDA-induced currents were sequentially recorded on the same aSMC with intermediate aCSF washing. k , Quantification for j ( N = 12 cells). l , Model diagram shows neural optogenetics (OG) and responding GCamp6 dynamic signals in co-culture. m , Representative live image of a ChR2 and GCamp6 double-positive neuron connecting with a GCamp6 and tdTomato double-positive aSMCs; n = 6 co-cultures. n , Sequential Ca 2+ images of aSMCs upon OG. o , Quantitative analysis of the effect of D-AP5 on Ca 2+ dynamics in aSMCs during OG, as shown in n ( N = 8 cells without OG; 5 cells with OG; 6 cells with OG plus D-AP5; cells were collected from three independent experiments). Data are the mean ± s.e.m; nested unpaired two-tailed t -test ( c and o ), one-way ANOVA with Bonferroni multiple-comparison post hoc tests ( g and h ).
D Ap5, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti hsdap5 bethyl laboratories cat  (Bethyl)
91
Bethyl rabbit polyclonal anti hsdap5 bethyl laboratories cat
a , Schematic showing the spatial arrangement of two electrodes, with the left one filled with glutamate (1 mM) for activating aSMCs through puff application, while the right one records membrane currents. b , A representative trace of glutamate-induced depolarizing currents at a holding potential of –40 mV in the absence (top) and presence (bottom) of <t>D-AP5</t> and NBQX. c , The statistical analysis for b ( N = 5 cells). d , e , Still frame images of Ca 2+ spark-like ( d ) and Ca 2+ wave ( e ) in aSMCs upon 500 μM ( d ) or 1 mM ( e ) glutamate stimulation (red, high; blue, low). f , Representative trace of Ca 2+ spark-like kinetics and Ca 2+ wave of d and e . g , h , Quantification of the frequencies of Ca 2+ spark-like events and Ca 2+ waves in aSMCs treated with vehicle ( n = 4 cells), glutamate alone ( g , 500 μM; h , 1 mM; n = 5 cells for each dose), or together with D-AP5 ( n = 5 cells). These cells were collected from three independent experiments. The baseline F0 was determined by averaging 10 images without Ca2+ sparks. A Ca2+ spark was identified as a localized increase in ΔF/F0 greater than 0.2. Ca2+ waves were defined by an ΔF/F0 elevation >0.2 that propagated for more than 20 μm. i , Schematic illustration depicts the spatial configuration of electrodes, with the left one containing NMDA (100 μM) for puffing. j , Exemplary traces of NMDA-evoked currents at a holding potential of –40 mV under Mg 2+ -free artificial cerebrospinal fluid (aCSF) conditions. The first (left) and second (right) NMDA-induced currents were sequentially recorded on the same aSMC with intermediate aCSF washing. k , Quantification for j ( N = 12 cells). l , Model diagram shows neural optogenetics (OG) and responding GCamp6 dynamic signals in co-culture. m , Representative live image of a ChR2 and GCamp6 double-positive neuron connecting with a GCamp6 and tdTomato double-positive aSMCs; n = 6 co-cultures. n , Sequential Ca 2+ images of aSMCs upon OG. o , Quantitative analysis of the effect of D-AP5 on Ca 2+ dynamics in aSMCs during OG, as shown in n ( N = 8 cells without OG; 5 cells with OG; 6 cells with OG plus D-AP5; cells were collected from three independent experiments). Data are the mean ± s.e.m; nested unpaired two-tailed t -test ( c and o ), one-way ANOVA with Bonferroni multiple-comparison post hoc tests ( g and h ).
Rabbit Polyclonal Anti Hsdap5 Bethyl Laboratories Cat, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dap5  (Proteintech)
90
Proteintech dap5
a , Schematic showing the spatial arrangement of two electrodes, with the left one filled with glutamate (1 mM) for activating aSMCs through puff application, while the right one records membrane currents. b , A representative trace of glutamate-induced depolarizing currents at a holding potential of –40 mV in the absence (top) and presence (bottom) of <t>D-AP5</t> and NBQX. c , The statistical analysis for b ( N = 5 cells). d , e , Still frame images of Ca 2+ spark-like ( d ) and Ca 2+ wave ( e ) in aSMCs upon 500 μM ( d ) or 1 mM ( e ) glutamate stimulation (red, high; blue, low). f , Representative trace of Ca 2+ spark-like kinetics and Ca 2+ wave of d and e . g , h , Quantification of the frequencies of Ca 2+ spark-like events and Ca 2+ waves in aSMCs treated with vehicle ( n = 4 cells), glutamate alone ( g , 500 μM; h , 1 mM; n = 5 cells for each dose), or together with D-AP5 ( n = 5 cells). These cells were collected from three independent experiments. The baseline F0 was determined by averaging 10 images without Ca2+ sparks. A Ca2+ spark was identified as a localized increase in ΔF/F0 greater than 0.2. Ca2+ waves were defined by an ΔF/F0 elevation >0.2 that propagated for more than 20 μm. i , Schematic illustration depicts the spatial configuration of electrodes, with the left one containing NMDA (100 μM) for puffing. j , Exemplary traces of NMDA-evoked currents at a holding potential of –40 mV under Mg 2+ -free artificial cerebrospinal fluid (aCSF) conditions. The first (left) and second (right) NMDA-induced currents were sequentially recorded on the same aSMC with intermediate aCSF washing. k , Quantification for j ( N = 12 cells). l , Model diagram shows neural optogenetics (OG) and responding GCamp6 dynamic signals in co-culture. m , Representative live image of a ChR2 and GCamp6 double-positive neuron connecting with a GCamp6 and tdTomato double-positive aSMCs; n = 6 co-cultures. n , Sequential Ca 2+ images of aSMCs upon OG. o , Quantitative analysis of the effect of D-AP5 on Ca 2+ dynamics in aSMCs during OG, as shown in n ( N = 8 cells without OG; 5 cells with OG; 6 cells with OG plus D-AP5; cells were collected from three independent experiments). Data are the mean ± s.e.m; nested unpaired two-tailed t -test ( c and o ), one-way ANOVA with Bonferroni multiple-comparison post hoc tests ( g and h ).
Dap5, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 1 3xflag iqub  (Addgene inc)
92
Addgene inc pcdna3 1 3xflag iqub
a , Schematic showing the spatial arrangement of two electrodes, with the left one filled with glutamate (1 mM) for activating aSMCs through puff application, while the right one records membrane currents. b , A representative trace of glutamate-induced depolarizing currents at a holding potential of –40 mV in the absence (top) and presence (bottom) of <t>D-AP5</t> and NBQX. c , The statistical analysis for b ( N = 5 cells). d , e , Still frame images of Ca 2+ spark-like ( d ) and Ca 2+ wave ( e ) in aSMCs upon 500 μM ( d ) or 1 mM ( e ) glutamate stimulation (red, high; blue, low). f , Representative trace of Ca 2+ spark-like kinetics and Ca 2+ wave of d and e . g , h , Quantification of the frequencies of Ca 2+ spark-like events and Ca 2+ waves in aSMCs treated with vehicle ( n = 4 cells), glutamate alone ( g , 500 μM; h , 1 mM; n = 5 cells for each dose), or together with D-AP5 ( n = 5 cells). These cells were collected from three independent experiments. The baseline F0 was determined by averaging 10 images without Ca2+ sparks. A Ca2+ spark was identified as a localized increase in ΔF/F0 greater than 0.2. Ca2+ waves were defined by an ΔF/F0 elevation >0.2 that propagated for more than 20 μm. i , Schematic illustration depicts the spatial configuration of electrodes, with the left one containing NMDA (100 μM) for puffing. j , Exemplary traces of NMDA-evoked currents at a holding potential of –40 mV under Mg 2+ -free artificial cerebrospinal fluid (aCSF) conditions. The first (left) and second (right) NMDA-induced currents were sequentially recorded on the same aSMC with intermediate aCSF washing. k , Quantification for j ( N = 12 cells). l , Model diagram shows neural optogenetics (OG) and responding GCamp6 dynamic signals in co-culture. m , Representative live image of a ChR2 and GCamp6 double-positive neuron connecting with a GCamp6 and tdTomato double-positive aSMCs; n = 6 co-cultures. n , Sequential Ca 2+ images of aSMCs upon OG. o , Quantitative analysis of the effect of D-AP5 on Ca 2+ dynamics in aSMCs during OG, as shown in n ( N = 8 cells without OG; 5 cells with OG; 6 cells with OG plus D-AP5; cells were collected from three independent experiments). Data are the mean ± s.e.m; nested unpaired two-tailed t -test ( c and o ), one-way ANOVA with Bonferroni multiple-comparison post hoc tests ( g and h ).
Pcdna3 1 3xflag Iqub, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d ap5  (Biosynth Carbosynth)
90
Biosynth Carbosynth d ap5
a , Schematic showing the spatial arrangement of two electrodes, with the left one filled with glutamate (1 mM) for activating aSMCs through puff application, while the right one records membrane currents. b , A representative trace of glutamate-induced depolarizing currents at a holding potential of –40 mV in the absence (top) and presence (bottom) of <t>D-AP5</t> and NBQX. c , The statistical analysis for b ( N = 5 cells). d , e , Still frame images of Ca 2+ spark-like ( d ) and Ca 2+ wave ( e ) in aSMCs upon 500 μM ( d ) or 1 mM ( e ) glutamate stimulation (red, high; blue, low). f , Representative trace of Ca 2+ spark-like kinetics and Ca 2+ wave of d and e . g , h , Quantification of the frequencies of Ca 2+ spark-like events and Ca 2+ waves in aSMCs treated with vehicle ( n = 4 cells), glutamate alone ( g , 500 μM; h , 1 mM; n = 5 cells for each dose), or together with D-AP5 ( n = 5 cells). These cells were collected from three independent experiments. The baseline F0 was determined by averaging 10 images without Ca2+ sparks. A Ca2+ spark was identified as a localized increase in ΔF/F0 greater than 0.2. Ca2+ waves were defined by an ΔF/F0 elevation >0.2 that propagated for more than 20 μm. i , Schematic illustration depicts the spatial configuration of electrodes, with the left one containing NMDA (100 μM) for puffing. j , Exemplary traces of NMDA-evoked currents at a holding potential of –40 mV under Mg 2+ -free artificial cerebrospinal fluid (aCSF) conditions. The first (left) and second (right) NMDA-induced currents were sequentially recorded on the same aSMC with intermediate aCSF washing. k , Quantification for j ( N = 12 cells). l , Model diagram shows neural optogenetics (OG) and responding GCamp6 dynamic signals in co-culture. m , Representative live image of a ChR2 and GCamp6 double-positive neuron connecting with a GCamp6 and tdTomato double-positive aSMCs; n = 6 co-cultures. n , Sequential Ca 2+ images of aSMCs upon OG. o , Quantitative analysis of the effect of D-AP5 on Ca 2+ dynamics in aSMCs during OG, as shown in n ( N = 8 cells without OG; 5 cells with OG; 6 cells with OG plus D-AP5; cells were collected from three independent experiments). Data are the mean ± s.e.m; nested unpaired two-tailed t -test ( c and o ), one-way ANOVA with Bonferroni multiple-comparison post hoc tests ( g and h ).
D Ap5, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eif4g2  (Proteintech)
93
Proteintech eif4g2
a , Schematic showing the spatial arrangement of two electrodes, with the left one filled with glutamate (1 mM) for activating aSMCs through puff application, while the right one records membrane currents. b , A representative trace of glutamate-induced depolarizing currents at a holding potential of –40 mV in the absence (top) and presence (bottom) of <t>D-AP5</t> and NBQX. c , The statistical analysis for b ( N = 5 cells). d , e , Still frame images of Ca 2+ spark-like ( d ) and Ca 2+ wave ( e ) in aSMCs upon 500 μM ( d ) or 1 mM ( e ) glutamate stimulation (red, high; blue, low). f , Representative trace of Ca 2+ spark-like kinetics and Ca 2+ wave of d and e . g , h , Quantification of the frequencies of Ca 2+ spark-like events and Ca 2+ waves in aSMCs treated with vehicle ( n = 4 cells), glutamate alone ( g , 500 μM; h , 1 mM; n = 5 cells for each dose), or together with D-AP5 ( n = 5 cells). These cells were collected from three independent experiments. The baseline F0 was determined by averaging 10 images without Ca2+ sparks. A Ca2+ spark was identified as a localized increase in ΔF/F0 greater than 0.2. Ca2+ waves were defined by an ΔF/F0 elevation >0.2 that propagated for more than 20 μm. i , Schematic illustration depicts the spatial configuration of electrodes, with the left one containing NMDA (100 μM) for puffing. j , Exemplary traces of NMDA-evoked currents at a holding potential of –40 mV under Mg 2+ -free artificial cerebrospinal fluid (aCSF) conditions. The first (left) and second (right) NMDA-induced currents were sequentially recorded on the same aSMC with intermediate aCSF washing. k , Quantification for j ( N = 12 cells). l , Model diagram shows neural optogenetics (OG) and responding GCamp6 dynamic signals in co-culture. m , Representative live image of a ChR2 and GCamp6 double-positive neuron connecting with a GCamp6 and tdTomato double-positive aSMCs; n = 6 co-cultures. n , Sequential Ca 2+ images of aSMCs upon OG. o , Quantitative analysis of the effect of D-AP5 on Ca 2+ dynamics in aSMCs during OG, as shown in n ( N = 8 cells without OG; 5 cells with OG; 6 cells with OG plus D-AP5; cells were collected from three independent experiments). Data are the mean ± s.e.m; nested unpaired two-tailed t -test ( c and o ), one-way ANOVA with Bonferroni multiple-comparison post hoc tests ( g and h ).
Eif4g2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d 2 amino 5 phosphonovaleric acid  (Alomone Labs)
93
Alomone Labs d 2 amino 5 phosphonovaleric acid
a , Schematic showing the spatial arrangement of two electrodes, with the left one filled with glutamate (1 mM) for activating aSMCs through puff application, while the right one records membrane currents. b , A representative trace of glutamate-induced depolarizing currents at a holding potential of –40 mV in the absence (top) and presence (bottom) of <t>D-AP5</t> and NBQX. c , The statistical analysis for b ( N = 5 cells). d , e , Still frame images of Ca 2+ spark-like ( d ) and Ca 2+ wave ( e ) in aSMCs upon 500 μM ( d ) or 1 mM ( e ) glutamate stimulation (red, high; blue, low). f , Representative trace of Ca 2+ spark-like kinetics and Ca 2+ wave of d and e . g , h , Quantification of the frequencies of Ca 2+ spark-like events and Ca 2+ waves in aSMCs treated with vehicle ( n = 4 cells), glutamate alone ( g , 500 μM; h , 1 mM; n = 5 cells for each dose), or together with D-AP5 ( n = 5 cells). These cells were collected from three independent experiments. The baseline F0 was determined by averaging 10 images without Ca2+ sparks. A Ca2+ spark was identified as a localized increase in ΔF/F0 greater than 0.2. Ca2+ waves were defined by an ΔF/F0 elevation >0.2 that propagated for more than 20 μm. i , Schematic illustration depicts the spatial configuration of electrodes, with the left one containing NMDA (100 μM) for puffing. j , Exemplary traces of NMDA-evoked currents at a holding potential of –40 mV under Mg 2+ -free artificial cerebrospinal fluid (aCSF) conditions. The first (left) and second (right) NMDA-induced currents were sequentially recorded on the same aSMC with intermediate aCSF washing. k , Quantification for j ( N = 12 cells). l , Model diagram shows neural optogenetics (OG) and responding GCamp6 dynamic signals in co-culture. m , Representative live image of a ChR2 and GCamp6 double-positive neuron connecting with a GCamp6 and tdTomato double-positive aSMCs; n = 6 co-cultures. n , Sequential Ca 2+ images of aSMCs upon OG. o , Quantitative analysis of the effect of D-AP5 on Ca 2+ dynamics in aSMCs during OG, as shown in n ( N = 8 cells without OG; 5 cells with OG; 6 cells with OG plus D-AP5; cells were collected from three independent experiments). Data are the mean ± s.e.m; nested unpaired two-tailed t -test ( c and o ), one-way ANOVA with Bonferroni multiple-comparison post hoc tests ( g and h ).
D 2 Amino 5 Phosphonovaleric Acid, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti eif4g antibody  (Novus Biologicals)
86
Novus Biologicals rabbit polyclonal anti eif4g antibody
a , Schematic showing the spatial arrangement of two electrodes, with the left one filled with glutamate (1 mM) for activating aSMCs through puff application, while the right one records membrane currents. b , A representative trace of glutamate-induced depolarizing currents at a holding potential of –40 mV in the absence (top) and presence (bottom) of <t>D-AP5</t> and NBQX. c , The statistical analysis for b ( N = 5 cells). d , e , Still frame images of Ca 2+ spark-like ( d ) and Ca 2+ wave ( e ) in aSMCs upon 500 μM ( d ) or 1 mM ( e ) glutamate stimulation (red, high; blue, low). f , Representative trace of Ca 2+ spark-like kinetics and Ca 2+ wave of d and e . g , h , Quantification of the frequencies of Ca 2+ spark-like events and Ca 2+ waves in aSMCs treated with vehicle ( n = 4 cells), glutamate alone ( g , 500 μM; h , 1 mM; n = 5 cells for each dose), or together with D-AP5 ( n = 5 cells). These cells were collected from three independent experiments. The baseline F0 was determined by averaging 10 images without Ca2+ sparks. A Ca2+ spark was identified as a localized increase in ΔF/F0 greater than 0.2. Ca2+ waves were defined by an ΔF/F0 elevation >0.2 that propagated for more than 20 μm. i , Schematic illustration depicts the spatial configuration of electrodes, with the left one containing NMDA (100 μM) for puffing. j , Exemplary traces of NMDA-evoked currents at a holding potential of –40 mV under Mg 2+ -free artificial cerebrospinal fluid (aCSF) conditions. The first (left) and second (right) NMDA-induced currents were sequentially recorded on the same aSMC with intermediate aCSF washing. k , Quantification for j ( N = 12 cells). l , Model diagram shows neural optogenetics (OG) and responding GCamp6 dynamic signals in co-culture. m , Representative live image of a ChR2 and GCamp6 double-positive neuron connecting with a GCamp6 and tdTomato double-positive aSMCs; n = 6 co-cultures. n , Sequential Ca 2+ images of aSMCs upon OG. o , Quantitative analysis of the effect of D-AP5 on Ca 2+ dynamics in aSMCs during OG, as shown in n ( N = 8 cells without OG; 5 cells with OG; 6 cells with OG plus D-AP5; cells were collected from three independent experiments). Data are the mean ± s.e.m; nested unpaired two-tailed t -test ( c and o ), one-way ANOVA with Bonferroni multiple-comparison post hoc tests ( g and h ).
Rabbit Polyclonal Anti Eif4g Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti dap5- (anti-nat1  (Becton Dickinson)
90
Becton Dickinson anti dap5- (anti-nat1
a , Schematic showing the spatial arrangement of two electrodes, with the left one filled with glutamate (1 mM) for activating aSMCs through puff application, while the right one records membrane currents. b , A representative trace of glutamate-induced depolarizing currents at a holding potential of –40 mV in the absence (top) and presence (bottom) of <t>D-AP5</t> and NBQX. c , The statistical analysis for b ( N = 5 cells). d , e , Still frame images of Ca 2+ spark-like ( d ) and Ca 2+ wave ( e ) in aSMCs upon 500 μM ( d ) or 1 mM ( e ) glutamate stimulation (red, high; blue, low). f , Representative trace of Ca 2+ spark-like kinetics and Ca 2+ wave of d and e . g , h , Quantification of the frequencies of Ca 2+ spark-like events and Ca 2+ waves in aSMCs treated with vehicle ( n = 4 cells), glutamate alone ( g , 500 μM; h , 1 mM; n = 5 cells for each dose), or together with D-AP5 ( n = 5 cells). These cells were collected from three independent experiments. The baseline F0 was determined by averaging 10 images without Ca2+ sparks. A Ca2+ spark was identified as a localized increase in ΔF/F0 greater than 0.2. Ca2+ waves were defined by an ΔF/F0 elevation >0.2 that propagated for more than 20 μm. i , Schematic illustration depicts the spatial configuration of electrodes, with the left one containing NMDA (100 μM) for puffing. j , Exemplary traces of NMDA-evoked currents at a holding potential of –40 mV under Mg 2+ -free artificial cerebrospinal fluid (aCSF) conditions. The first (left) and second (right) NMDA-induced currents were sequentially recorded on the same aSMC with intermediate aCSF washing. k , Quantification for j ( N = 12 cells). l , Model diagram shows neural optogenetics (OG) and responding GCamp6 dynamic signals in co-culture. m , Representative live image of a ChR2 and GCamp6 double-positive neuron connecting with a GCamp6 and tdTomato double-positive aSMCs; n = 6 co-cultures. n , Sequential Ca 2+ images of aSMCs upon OG. o , Quantitative analysis of the effect of D-AP5 on Ca 2+ dynamics in aSMCs during OG, as shown in n ( N = 8 cells without OG; 5 cells with OG; 6 cells with OG plus D-AP5; cells were collected from three independent experiments). Data are the mean ± s.e.m; nested unpaired two-tailed t -test ( c and o ), one-way ANOVA with Bonferroni multiple-comparison post hoc tests ( g and h ).
Anti Dap5 (Anti Nat1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a , Schematic showing the spatial arrangement of two electrodes, with the left one filled with glutamate (1 mM) for activating aSMCs through puff application, while the right one records membrane currents. b , A representative trace of glutamate-induced depolarizing currents at a holding potential of –40 mV in the absence (top) and presence (bottom) of D-AP5 and NBQX. c , The statistical analysis for b ( N = 5 cells). d , e , Still frame images of Ca 2+ spark-like ( d ) and Ca 2+ wave ( e ) in aSMCs upon 500 μM ( d ) or 1 mM ( e ) glutamate stimulation (red, high; blue, low). f , Representative trace of Ca 2+ spark-like kinetics and Ca 2+ wave of d and e . g , h , Quantification of the frequencies of Ca 2+ spark-like events and Ca 2+ waves in aSMCs treated with vehicle ( n = 4 cells), glutamate alone ( g , 500 μM; h , 1 mM; n = 5 cells for each dose), or together with D-AP5 ( n = 5 cells). These cells were collected from three independent experiments. The baseline F0 was determined by averaging 10 images without Ca2+ sparks. A Ca2+ spark was identified as a localized increase in ΔF/F0 greater than 0.2. Ca2+ waves were defined by an ΔF/F0 elevation >0.2 that propagated for more than 20 μm. i , Schematic illustration depicts the spatial configuration of electrodes, with the left one containing NMDA (100 μM) for puffing. j , Exemplary traces of NMDA-evoked currents at a holding potential of –40 mV under Mg 2+ -free artificial cerebrospinal fluid (aCSF) conditions. The first (left) and second (right) NMDA-induced currents were sequentially recorded on the same aSMC with intermediate aCSF washing. k , Quantification for j ( N = 12 cells). l , Model diagram shows neural optogenetics (OG) and responding GCamp6 dynamic signals in co-culture. m , Representative live image of a ChR2 and GCamp6 double-positive neuron connecting with a GCamp6 and tdTomato double-positive aSMCs; n = 6 co-cultures. n , Sequential Ca 2+ images of aSMCs upon OG. o , Quantitative analysis of the effect of D-AP5 on Ca 2+ dynamics in aSMCs during OG, as shown in n ( N = 8 cells without OG; 5 cells with OG; 6 cells with OG plus D-AP5; cells were collected from three independent experiments). Data are the mean ± s.e.m; nested unpaired two-tailed t -test ( c and o ), one-way ANOVA with Bonferroni multiple-comparison post hoc tests ( g and h ).

Journal: Nature Neuroscience

Article Title: Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling

doi: 10.1038/s41593-023-01515-0

Figure Lengend Snippet: a , Schematic showing the spatial arrangement of two electrodes, with the left one filled with glutamate (1 mM) for activating aSMCs through puff application, while the right one records membrane currents. b , A representative trace of glutamate-induced depolarizing currents at a holding potential of –40 mV in the absence (top) and presence (bottom) of D-AP5 and NBQX. c , The statistical analysis for b ( N = 5 cells). d , e , Still frame images of Ca 2+ spark-like ( d ) and Ca 2+ wave ( e ) in aSMCs upon 500 μM ( d ) or 1 mM ( e ) glutamate stimulation (red, high; blue, low). f , Representative trace of Ca 2+ spark-like kinetics and Ca 2+ wave of d and e . g , h , Quantification of the frequencies of Ca 2+ spark-like events and Ca 2+ waves in aSMCs treated with vehicle ( n = 4 cells), glutamate alone ( g , 500 μM; h , 1 mM; n = 5 cells for each dose), or together with D-AP5 ( n = 5 cells). These cells were collected from three independent experiments. The baseline F0 was determined by averaging 10 images without Ca2+ sparks. A Ca2+ spark was identified as a localized increase in ΔF/F0 greater than 0.2. Ca2+ waves were defined by an ΔF/F0 elevation >0.2 that propagated for more than 20 μm. i , Schematic illustration depicts the spatial configuration of electrodes, with the left one containing NMDA (100 μM) for puffing. j , Exemplary traces of NMDA-evoked currents at a holding potential of –40 mV under Mg 2+ -free artificial cerebrospinal fluid (aCSF) conditions. The first (left) and second (right) NMDA-induced currents were sequentially recorded on the same aSMC with intermediate aCSF washing. k , Quantification for j ( N = 12 cells). l , Model diagram shows neural optogenetics (OG) and responding GCamp6 dynamic signals in co-culture. m , Representative live image of a ChR2 and GCamp6 double-positive neuron connecting with a GCamp6 and tdTomato double-positive aSMCs; n = 6 co-cultures. n , Sequential Ca 2+ images of aSMCs upon OG. o , Quantitative analysis of the effect of D-AP5 on Ca 2+ dynamics in aSMCs during OG, as shown in n ( N = 8 cells without OG; 5 cells with OG; 6 cells with OG plus D-AP5; cells were collected from three independent experiments). Data are the mean ± s.e.m; nested unpaired two-tailed t -test ( c and o ), one-way ANOVA with Bonferroni multiple-comparison post hoc tests ( g and h ).

Article Snippet: For in vivo experiments, D-AP5 (500 μM), COX2 antagonist (100 μM, NS-398, MedChemExpress) or PAX (100 μM) was administered by intracisternal injection 15 min before whisker stimulation or 2P optogenetic stimulation.

Techniques: Membrane, Optogenetics, Co-Culture Assay, Two Tailed Test, Comparison

(a) Schematic graph of the experimental workflow of broad 2 P optogenetics (b) Two-photon image of a p-arteriole (labeled with Hydrazide Alex 633, white arrows) at a cortical depth of 128 μm and surrounding ChR2-mCherry positive glutamatergic axons (green arrows). (c) Still frame images of p-arterioles before and during photostimulation in the littermate control ( Grin1 f/f ) and aSMC-cKO Grin1 mice ( SMACreER:Grin1 f/f ). The GCamp6s intensity further enhanced in the same axons when illumination power switched from 25 mW (white arrow) to 80 mW laser (green arrow). The white dashed lines outline the basal arteriole calibers. (d) Time course of changes in p-arteriolar diameter in response to 1100-nm laser at 25 mW and the following 960-nm laser with increasing power in c. (e) Maximum dilation amplitudes in c (magenta, n = 8 p-arterioles from 6 mice) or cistern magna injection of D-AP5 (100 μM, green, n = 4 p-arterioles from 3 mice) in control mice or aSMC-cKO Grin1 mice (orange, n = 6 p-arterioles from 5 mice). Data are shown as the mean ± s.e.m.; nested, one-way ANOVA after a post hoc Bonferroni multiple comparison adjustments (e) .

Journal: Nature Neuroscience

Article Title: Synaptic-like transmission between neural axons and arteriolar smooth muscle cells drives cerebral neurovascular coupling

doi: 10.1038/s41593-023-01515-0

Figure Lengend Snippet: (a) Schematic graph of the experimental workflow of broad 2 P optogenetics (b) Two-photon image of a p-arteriole (labeled with Hydrazide Alex 633, white arrows) at a cortical depth of 128 μm and surrounding ChR2-mCherry positive glutamatergic axons (green arrows). (c) Still frame images of p-arterioles before and during photostimulation in the littermate control ( Grin1 f/f ) and aSMC-cKO Grin1 mice ( SMACreER:Grin1 f/f ). The GCamp6s intensity further enhanced in the same axons when illumination power switched from 25 mW (white arrow) to 80 mW laser (green arrow). The white dashed lines outline the basal arteriole calibers. (d) Time course of changes in p-arteriolar diameter in response to 1100-nm laser at 25 mW and the following 960-nm laser with increasing power in c. (e) Maximum dilation amplitudes in c (magenta, n = 8 p-arterioles from 6 mice) or cistern magna injection of D-AP5 (100 μM, green, n = 4 p-arterioles from 3 mice) in control mice or aSMC-cKO Grin1 mice (orange, n = 6 p-arterioles from 5 mice). Data are shown as the mean ± s.e.m.; nested, one-way ANOVA after a post hoc Bonferroni multiple comparison adjustments (e) .

Article Snippet: For in vivo experiments, D-AP5 (500 μM), COX2 antagonist (100 μM, NS-398, MedChemExpress) or PAX (100 μM) was administered by intracisternal injection 15 min before whisker stimulation or 2P optogenetic stimulation.

Techniques: Optogenetics, Labeling, Control, Injection, Comparison