Journal: BMC Veterinary Research

Article Title: Long-term changes of Th17 and regulatory T cells in peripheral blood of dogs with spinal cord injury after intervertebral disc herniation

doi: 10.1186/s12917-023-03647-8

Figure Lengend Snippet: Illustration of a Treg and a Th17 cell analysis using flow cytometry Results of Treg and Th17 cell analysis of a dog after recovery of spinal cord injury using MACSQuant analysis software In the prior performed isotype controls, limits were set at approx. 2% of the cells [ , – ]. This limit was transferred to the test fractions (pink lines) in each case to detect the percentages of FOXP3 (a) or IL17 (b) positive cells, which are located to the right of the pink line Absolute cell numbers were subsequently calculated to make the analyses comparable a) The graphic displays the percentage of FOXP3 positive cells for detection of Treg cells. 9.56% FOXP3 positive cells were identified b) The graphic displays the percentage of IL17 positive cells for detection of Th17. 16.20% IL17 positive cells were identified. The results of a stimulated Th17 cell fraction are shown

Article Snippet: For cell fixation, a flow cytometry fixation buffer (R&D Systems®, Minneapolis, Minnesota, USA) was used.

Techniques: Cell Analysis, Flow Cytometry, Software

Journal: Neuro-Oncology

Article Title: Agonist OX40 immunotherapy improves survival in glioma-bearing mice and is complementary with vaccination with irradiated GM-CSF–expressing tumor cells

doi: 10.1093/neuonc/nox125

Figure Lengend Snippet: Th1 vs Th2 BILs. (A) Flow cytometry demonstrating percent expression of T-bet, IFN-γ, and IL-12R β2 on CD4+ lymphocytes 18 days after GL261 tumor implantation by treatment group. Combination immunotherapy is associated with significant increases in all 3 Th1 markers. (***P < 0.0005, **P < 0.005, *P < 0.05; absence of an asterisk denotes not significant comparison.) (B) Flow cytometry demonstrating percent expression of GATA3, the IL-4 receptor, and IL-5 on CD4+ lymphocytes 18 days after GL261 tumor implantation by treatment group. Combination immunotherapy is associated with significant decreases in all 3 Th2 markers. (**P < 0.005, *P < 0.05, absence of an asterisk denotes not significant comparison.)

Article Snippet: For the analysis of T helper cell subsets, we used Multi-Color Flow Cytometry Kits (R&D Systems) for mouse Th1 cells (conjugated antibodies to T-bet-PerCP, IFN-gamma-fluorescein, IL-12 R beta 2-APC, and CD4-PE) and Th2 cells (conjugated antibodies to CD4-PerCP, IL-4 R-fluorescein, STAT6-APC, and IL-5-PE).

Techniques: Flow Cytometry, Expressing, Tumor Implantation, Comparison

Journal: Neuro-Oncology

Article Title: Agonist OX40 immunotherapy improves survival in glioma-bearing mice and is complementary with vaccination with irradiated GM-CSF–expressing tumor cells

doi: 10.1093/neuonc/nox125

Figure Lengend Snippet: Day 18 flow cytometry of BILs shows that vaccination improves the intratumoral CD8+/FoxP3+ lymphocyte ratio, which is little affected by treatment with anti-OX40 immunotherapy. (**P < 0.005, *P < 0.05; absence of an asterisk denotes not significant comparison.)

Article Snippet: For the analysis of T helper cell subsets, we used Multi-Color Flow Cytometry Kits (R&D Systems) for mouse Th1 cells (conjugated antibodies to T-bet-PerCP, IFN-gamma-fluorescein, IL-12 R beta 2-APC, and CD4-PE) and Th2 cells (conjugated antibodies to CD4-PerCP, IL-4 R-fluorescein, STAT6-APC, and IL-5-PE).

Techniques: Flow Cytometry, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Reduced Dendritic Cells Expressing CD200R1 in Children with Inflammatory Bowel Disease: Correlation with Th17 and Regulatory T Cells

doi: 10.3390/ijms161226143

Figure Lengend Snippet: Representative flow cytometry data to illustrate the gating strategy for FACS analysis of DC subsets. ( A ) Dendritic cell HLA-DR + Lin − cells were gated ( R1 ), and myeloid CD11c + mDCs ( R2 ), plasmacytoid CD123 + pDCs ( R3 ) were determined as a percentage of the total PBMC ( B ). Dot plots to illustrate the expression of CD200 and CD200R1 on the myeloid dendritic cell (mDC) subset of healthy controls and IBD patients ( C , D ) and on and plasmacytoid DCs (pDCs) of healthy controls and IBD patients ( E , F ).

Article Snippet: For regulatory T cell staining, 1 × 10 6 cells were resuspended in 100 μL flow cytometry staining buffer (R&D Systems, Minneapolis, MN, USA).

Techniques: Flow Cytometry, Expressing