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Image Search Results
Journal: Molecular cell
Article Title: In Vivo Evidence for Serine Biosynthesis-Defined Sensitivity of Lung Metastasis, but Not of Primary Breast Tumors, to mTORC1 Inhibition
doi: 10.1016/j.molcel.2020.11.027
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: The drug was dissolved at a concentration of 0.0002 mg per μL. α-Cyano-4-hydroxycinnamic acid (Merck Sigma, C2020) acute treatment consisted of two injections in the last two days of the experiment to only investigate short-term effects of the molecular mechanisms triggered by pyruvate uptake inhibition on the phosphorylation of mTORC1 targets. α-Cyano-4-hydroxycinnamic acid (60 mg per kg) was intraperitoneally injected. α-Cyano-4-hydroxycinnamic acid was dissolved in a mix containing 1.5% DMSO, 60%
Techniques: Plasmid Preparation, Recombinant, Lysis, Bicinchoninic Acid Protein Assay, Western Blot, Software