Journal: Frontiers in Pharmacology

Article Title: Akt Regulated Phosphorylation of GSK-3β/Cyclin D1, p21 and p27 Contributes to Cell Proliferation Through Cell Cycle Progression From G1 to S/G2M Phase in Low-Dose Arsenite Exposed HaCat Cells

doi: 10.3389/fphar.2019.01176

Figure Lengend Snippet: Repeated low-dose NaAsO 2 exposure induced phosphorylated protein expression of GSK-3β/cyclinD1, p21 and p27 was regulated by AKT. Protein expression was detected by the Western blot analysis. (A) Images are the representative results of three separate experiments. Quantitative graphs ( n = 3) showed the relative intensity of the target proteins compare with β-actin, including p -AKT (B) , p -GSK-3β (C) , total cyclin D1 (D) , p -cyclin D1 (E) , p -p21 (F) , p -p27 (G) , total p21 (H) and total p27 (I) . NaAsO 2 exposure induced increased expression of p -AKT, p -GSK-3β, total cyclin D1, p -p21, and p -p27, but decreased expression of p -cyclin D1, total p21, and total p27. Treatment of MK2206 significantly reversed the expression of all of the above proteins. Significant difference was defined as p less than 0.05. a, vs. the corresponding 0 μM group; b, vs. the corresponding 0.05 μM group; c, vs. the MK2206(-) group of the same NaAsO 2 concentration.

Article Snippet: Antibodies against p -Akt (Ser473, #9271), Akt (#4685S), GSK-3β (#5676), p -cyclin D1 (Thr286, #3300), p21 (#2947), p27 (#3686), and MMP9 (#13667) were purchased from Cell Signaling Technology, USA.

Techniques: Expressing, Western Blot, Concentration Assay