Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: Hypoxia-CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia.

doi: 10.1177/1535370220960675

Figure Lengend Snippet: Figure 3. Effects of hypoxia on CXCL6 and CXCL2 gene expression in co-culture systems. (A) The difference of CXCL6 gene expression between censored (n ¼ 95) and relapse/refractory (n ¼ 247) patients in TARGET database. (B to E) The gene expression of CXCL6 and CXCL2 of MSCs and HUAECs in the co-culture systems were measured by the co-culture/mono culture ratio. (B and D) MSC þ THP-1/MSC; (C and E) HUAEC þ THP-1/HUAEC. *P < 0.05, **P < 0.01, ***P < 0.001, and “ns” for no statistical significance. (A color version of this figure is available in the online journal.)

Article Snippet: The cytokine levels in the supernatant of each group were respectively detected by the human CXCL6 ELISA kit (BOSTER, Wuhan, China) and human CXCL2 ELISA Kit (MULTI SCIENCES, Hangzhou, China) according to the manufacturer’s protocol.

Techniques: Gene Expression, Co-Culture Assay

Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: Hypoxia-CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia.

doi: 10.1177/1535370220960675

Figure Lengend Snippet: Figure 4. CXCL6 and CXCL2 content in the supernatant of hypoxia and THP-1 co-culture systems. (A and B) CXCL6 content in supernatant. (C and D) CXCL2 content in supernatant. *P < 0.05, **P < 0.01, and ***P < 0.001. (A color version of this figure is available in the online journal.)

Article Snippet: The cytokine levels in the supernatant of each group were respectively detected by the human CXCL6 ELISA kit (BOSTER, Wuhan, China) and human CXCL2 ELISA Kit (MULTI SCIENCES, Hangzhou, China) according to the manufacturer’s protocol.

Techniques: Co-Culture Assay

Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: Hypoxia-CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia.

doi: 10.1177/1535370220960675

Figure Lengend Snippet: Figure 5. The effects of CXCL6 and CXCL2 on the proliferation and migration of MSCs and HUAECs. (A and B) The effects of CXCL6 (A) and CXCL2 (B). Among them, (a and b) described the effects of cytokines on MSCs and (c and d) described the effects of cytokines on HUAECs. (a and c) showed the effects of different concentrations of cytokines on target cells. (b and d) showed the time-dependent effect of cytokines on target cells. (C) The chemotaxis of CXCL6 and CXCL2 to MSCs and HUAECs respectively. The concentration of CXCL6 was 50 ng/mL and of CXCL2 was 100 ng/mL. Scale ¼ 100 mm. (A color version of this figure is available in the online journal.)

Article Snippet: The cytokine levels in the supernatant of each group were respectively detected by the human CXCL6 ELISA kit (BOSTER, Wuhan, China) and human CXCL2 ELISA Kit (MULTI SCIENCES, Hangzhou, China) according to the manufacturer’s protocol.

Techniques: Migration, Chemotaxis Assay, Concentration Assay

Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: Hypoxia-CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia.

doi: 10.1177/1535370220960675

Figure Lengend Snippet: Figure 6. Expression of cleaved-caspase3 in MSCs and HUAECs. (A) Expression of cleaved-caspase-3 (green fluorescence) measured by immunofluorescence method increased significantly in hypoxia (1% O2) for 48 h, but decreased after CXCL6 was supplied. The reversion depended on the concentration of CXCL6. MSCs were pictured at 20 water objective and HUAECs were pictured at 20 air objective. The heatmap represented the change of average fluorescence intensity in each group. (B) Western blot method verified the above results. The histograms (MSCs on the left and HUAECs on the right) represented the relative gray value of each group. (A color version of this figure is available in the online journal.)

Article Snippet: The cytokine levels in the supernatant of each group were respectively detected by the human CXCL6 ELISA kit (BOSTER, Wuhan, China) and human CXCL2 ELISA Kit (MULTI SCIENCES, Hangzhou, China) according to the manufacturer’s protocol.

Techniques: Expressing, Fluorescence, Immunofluorescence, Concentration Assay, Western Blot

Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: Hypoxia-CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia.

doi: 10.1177/1535370220960675

Figure Lengend Snippet: Figure 7. Angiogenesis ability of vascular endothelial cells authenticated in vitro. (A) HUAECs in standard culture conditions, CoCl2 simulate hypoxia and hypoxia plus CXCL6 or CM conditions in vitro were used to simulated angiogenesis. HUVECs tubule forming experiment was taken as positive control. (B to E) Quantitative comparison of the number of master junction and meshes as well as total meshes area and total segments length. Scale ¼ 200 mm. (A color version of this figure is available in the online journal.)

Article Snippet: The cytokine levels in the supernatant of each group were respectively detected by the human CXCL6 ELISA kit (BOSTER, Wuhan, China) and human CXCL2 ELISA Kit (MULTI SCIENCES, Hangzhou, China) according to the manufacturer’s protocol.

Techniques: In Vitro, Positive Control, Comparison

Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: Hypoxia-CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia.

doi: 10.1177/1535370220960675

Figure Lengend Snippet: Figure 8. Correlation between CXCL6 gene expression and HIF-1a gene expression in AML patients. The original data were downloaded from Cbioporta (https:// www.cbioportal.org/datasets), n ¼ 323. (A color version of this figure is available in the online journal.)

Article Snippet: The cytokine levels in the supernatant of each group were respectively detected by the human CXCL6 ELISA kit (BOSTER, Wuhan, China) and human CXCL2 ELISA Kit (MULTI SCIENCES, Hangzhou, China) according to the manufacturer’s protocol.

Techniques: Gene Expression

Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: Hypoxia-CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia.

doi: 10.1177/1535370220960675

Figure Lengend Snippet: Figure 9. Regulation of CXCL6 expression by HIF-1a. (A) MSCs or HUAECs were treated with CoCl2 (200 mmol/L), with or without BAY87-2243 (1 mmol/L), and the protein expression of HIF-1a was detected by Western blot method after 24 or 48 h. (B) The relative expression of HIF-1a in three independent experiments was statistically analyzed. The x-axis coordinates represented CoCl2()/BAY(), CoCl2(þ)/BAY(), CoCl2(þ)/BAY(þ), respectively. (a) MSC–24 h; (b) MSC–48 h; (c) HUAEC–24 h; (d) HUAEC–48 h. (C) MSCs and HUAECs were cultured for 24 or 48 h under the condition of 1% O2, with or without BAY87-2243 (1 mmol/L). The relative expression of CXCL6 mRNA was detected by real-time PCR. (a) MSC–24 h; (b) MSC–48 h; (c) HUAEC–24 h; (d) HUAEC–48 h. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (A color version of this figure is available in the online journal.)

Article Snippet: The cytokine levels in the supernatant of each group were respectively detected by the human CXCL6 ELISA kit (BOSTER, Wuhan, China) and human CXCL2 ELISA Kit (MULTI SCIENCES, Hangzhou, China) according to the manufacturer’s protocol.

Techniques: Expressing, Western Blot, Cell Culture, Real-time Polymerase Chain Reaction

Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: Hypoxia-CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia.

doi: 10.1177/1535370220960675

Figure Lengend Snippet: Figure 11. CXCL6 activate mTOR in MSCs and HUAECs. (A) CoCl2 (200 mmol/L) was used to treat MSCs or HUAECs for 24 h with or without CXCL6 of 50 ng/Ml. (B) The statistical results of three groups of independent experiments, **P < 0.01. (A color version of this figure is available in the online journal.)

Article Snippet: The cytokine levels in the supernatant of each group were respectively detected by the human CXCL6 ELISA kit (BOSTER, Wuhan, China) and human CXCL2 ELISA Kit (MULTI SCIENCES, Hangzhou, China) according to the manufacturer’s protocol.

Techniques:

Journal: Experimental biology and medicine (Maywood, N.J.)

Article Title: Hypoxia-CXCL6 axis affects arteriolar niche remodeling in acute myeloid leukemia.

doi: 10.1177/1535370220960675

Figure Lengend Snippet: Figure 10. Negative feedback regulation of CXCL6 on HIF-1a. (A) MSCs and HUAECs were cultured for 24 or 48 h under 1% O2 condition with or without CXCL6 (50 ng/mL). The relative mRNA expression of HIF-1a was detected by real-time PCR. (a) MSC–24 h; (b) MSC–48 h; (c) HUAEC–24 h; (d) HUAEC–48 h. (B) MSCs or HUAECs were treated with CoCl2, with or without CXCL6 (50 ng/mL). After 24 or 48 h, the expression of HIF-1a protein was detected by Western blot method. (C) The relative expression of HIF-1a protein in three independent experiments was statistically analyzed, and the x-axis coordinates were respectively representative CoCl2()/CXCL6(), CoCl2(þ)/CXCL6(), CoCl2(þ)/CXCL6(þ), (a) MSC–24 h; (b) MSC–48 h; (c) HUAEC–24 h; (d) HUAEC–48 h. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and “ns” for no statistical significance. (A color version of this figure is available in the online journal.)

Article Snippet: The cytokine levels in the supernatant of each group were respectively detected by the human CXCL6 ELISA kit (BOSTER, Wuhan, China) and human CXCL2 ELISA Kit (MULTI SCIENCES, Hangzhou, China) according to the manufacturer’s protocol.

Techniques: Cell Culture, Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Cell reports

Article Title: IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury

doi: 10.1016/j.celrep.2022.111848

Figure Lengend Snippet: Schematic representation of IL-17/CXCL5 signaling in chronically injured cerebral endothelia (A). TRAP-qPCR fold expression compared with average fpkm of top DEGs from white matter endothelia (*adjusted p < 0.05) (B). Weight-adjusted ELISA values (pg/mL) for murine CXCL5 in retro-orbital blood samples from CFD (black) and HFD (red) animals (n = 4/grp, p = 0.0355) (C). Immunofluorescence labeling for IL-17Rb (green, D) and CXCL5 (green, E) is absent in white matter vasculature of Tie2-Cre;tdTomato mice on CFD (left panels) and abundant in white matter vasculature of Tie2-Cre;tdTomato mice on HFD (right panels). Single-channel labeling for IL17Rb (bottom panels, D) and CXCL5 (bottom panels, E) show heterogeneous endothelial expression. Labeling for GLUT-1 (blue), CXCL5 (red), and PDGFRα (green) at 7 days post-stroke in animals on CFD (left) and HFD (right). Inset boxes from the peri-infarct tissue (top) masked for GLUT-1 (white) with only co-localized CXCL5 (purple) (bottom). Graph of percentage of co-localized CXCL5+/GLUT-1+ voxels (****p < 0.0001) (F). Error bars represent S.E.M. Scale bars: 50 μm (F), 20 μm (D), and 10 μm (E).

Article Snippet: A plasmid containing the open reading frame of the murine CXCL5 sequence with a 3′ stop codon was purchased from Origene (#MR200761).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Labeling

Journal: Cell reports

Article Title: IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury

doi: 10.1016/j.celrep.2022.111848

Figure Lengend Snippet: Human brain microvascular endothelial cells were stimulated with IL-17 ligands A–E (250 ng/mL) and CXCL5 levels measured in conditioned media 48 h after stimulation (*p = 0.0372 by Kruskal-Wallis H test; **post-hoc comparison for IL-17B versus no ligand, adjusted p = 0.0178) (A). Phalloidin+ cellular area in O4+ OPCs grown in vitro exposed to vehicle (top panel) or recombinant CXCL5 (bottom panel) for 48 h (p < 0.0001, F = 9.82 by one-way ANOVA) (B). Approach for CXCL5 transgenic-viral gain of function in subcortical white matter of Tie2-Cre;tdTomato mice (top panel) (C). PDGFRα+ OPC (green) labeling in GFP-transduced Tie2-Cre;tdTomato mice (red, left panel) and CXCL5-GFP-transduced Tie2-Cre;tdTomato mice (right panel). Representative masked cellular profiles of PDGFRα+ cell area (bottom panels). Schematic of anti-IL-17B antibody treatment (top panel) (D). PDGFRα+ OPC (green) labeling in control IgG-treated Tie2-Cre:tdT mice (left panel) and anti-IL-17B IgG-treated Tie2-Cre:tdT mice (right panel). Representative masked cellular profiles of PDGFRα+ cell area (bottom panels). Proportion of OPCs per unit distance from vessel (0–35 μm) in each condition (total measured cell number per condition in parentheses) (E). Average distance of OPCs to vessel (***p = 0.0005, F = 6.06 by one-way ANOVA; **adjusted p = 0.0039; *adjusted p = 0.0168) (F). Average in vivo PDGFRα+ OPC cell area (**p = 0.0068, F = 7.38 by one-way ANOVA; **adjusted p = 0.002) (G). Graph of co-localized CXCL5+/GLUT-1+ voxels in anti-IL-17B IgG-treated animals (n = 4/grp; *p = 0.018) (H). Error bars represent S.E.M. Scale bars: 10 μm

Article Snippet: A plasmid containing the open reading frame of the murine CXCL5 sequence with a 3′ stop codon was purchased from Origene (#MR200761).

Techniques: Comparison, In Vitro, Recombinant, Transgenic Assay, Labeling, Control, In Vivo

Journal: Cell reports

Article Title: IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury

doi: 10.1016/j.celrep.2022.111848

Figure Lengend Snippet: Plasma levels of log 10 -CXCL5 in ASPIRE cohort subjects separated by detectable plasma IL-17B (n = 32; median 1043.0 pg/mL) compared with those with undetectable plasma IL-17B (n = 99; median 515.3 pg/mL; *p < 0.0001). Plasma log 10 -CXCL5 levels in subjects with MRI-confirmed acute microvascular ischemia (IL-17B + subjects; n = 9; 978.2 pg/mL versus IL-17B− subjects; n = 24; 539.7 pg/mL) (**p = 0.0157) (A). Ordinal shift analysis of modified Fazekas scale scores from plasma IL-17B+ and IL-17B− subjects (p < 0.0001) (B). Representative immunohistochemical detection of CXCL5 in human frontal white matter vasculature in subjects with cerebrovascular pathology (C). Percentage of CXCL5+ vessel segments in peri-ventricular white matter (n = 10) (p = 0.0005). Error bars represent S.E.M. Scale bar: 10 μm

Article Snippet: A plasmid containing the open reading frame of the murine CXCL5 sequence with a 3′ stop codon was purchased from Origene (#MR200761).

Techniques: Modification, Immunohistochemical staining

Journal: Cell reports

Article Title: IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury

doi: 10.1016/j.celrep.2022.111848

Figure Lengend Snippet:

Article Snippet: A plasmid containing the open reading frame of the murine CXCL5 sequence with a 3′ stop codon was purchased from Origene (#MR200761).

Techniques: Blocking Assay, Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Luminex, Hybridization, Plasmid Preparation, Biomarker Assay, Control, Software

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Carbon dioxide alleviates platelet storage lesions via stimulating fatty acid metabolism and reducing platelet glucose consumption

doi: 10.1016/j.rpth.2025.102681

Figure Lengend Snippet: Carbon dioxide (CO 2 ) inhibited platelet activation and apoptosis during storage. (A) The percentage of CD62P-positive platelets was determined by flow cytometry. (B) Platelet factor 4 (PF4) concentration was determined by enzyme-linked immunosorbent assay. (C) The percentage of annexin V-positive platelets was determined by flow cytometry. (D) The mean fluorescence intensity (MFI) of CD42b was determined by flow cytometry ( n = 6). n.s., not significant, ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001, ∗∗∗∗ P < .0001.

Article Snippet: To determine PF4 concentration, 200 μL of supernatant from stored APCs (300 μL, 1 × 10 9 /mL) was processed following the PF4 ELISA Kit instructions (Boster).

Techniques: Activation Assay, Flow Cytometry, Concentration Assay, Enzyme-linked Immunosorbent Assay, Fluorescence

Journal: Molecular Medicine

Article Title: Cytokine modulation in pelvic organ prolapse and urinary incontinence: from molecular insights to therapeutic targets

doi: 10.1186/s10020-024-00989-3

Figure Lengend Snippet: The pro- and anti-inflammatory cytokines in UI clinical and preclinical research. Symbols ↑ indicated up-regulation, ↓ down-regulation, and ↔ no change

Article Snippet: The feasibility of periurethral injection of CXCL-12 has been documented, resulting in enhancements in both erectile dysfunction and UI (Zambon et al. ).

Techniques: Injection, Functional Assay, Migration, In Vivo