Journal: Journal of Translational Medicine

Article Title: Wnt signaling in age-related macular degeneration: human macular tissue and mouse model

doi: 10.1186/s12967-015-0683-x

Figure Lengend Snippet: Western blot analysis of key components of the Wnt pathway in mouse retina. a Phosphorylated LRP6 and non-phosphorylated β-catenin were higher in the retina of Ccl2 − / − /Cx3cr1 − / − /rd8 compared to wildtype B6N mice. Phosphorylated LRP6 and non-phosphorylated β-catenin were measured in retinal homogenates of mice treated with the anti-LRP6 antibody or control mIgG. Reduced phosphorylated LRP6 and non-phosphorylated β-catenin were observed after the anti-LRP6 antibody treatment (n = 3 from each condition). b Quantitative analysis of 3 experiments, *: <0.05

Article Snippet: The dark-adapted ERG was recorded for Ccl2 − / − /Cx3cr1 gfp/gfp (LKC Technologies, Gaithersburg, MD, USA) [ ].

Techniques: Western Blot, Control

Journal: Journal of Translational Medicine

Article Title: Wnt signaling in age-related macular degeneration: human macular tissue and mouse model

doi: 10.1186/s12967-015-0683-x

Figure Lengend Snippet: Slower progression or alleviation of retinal lesions in the anti-LRP6 treated eyes as compared to the control in both models. a Representative fundoscopic images of Ccl2 − / − /Cx3cr1 − / − /rd8 . Arrows indicate the yellowish deep retinal lesions. b Comparison of the average retinal lesion scores between treated and untreated eyes of 35 Ccl2 − / − /Cx3cr1 − / − /rd8 mice. Error bar mean ± SEM; *: p < 0.01. c Representative fundoscopic images of Ccl2 − / − /Cx3cr1 gfp/gfp . Arrows indicate the yellowish deep retinal lesions. In Ccl2 − / − /Cx3cr1 gfp/gfp , only 5 out of 10 treated eyes but 7 out of 10 control eyes showed lesion progression

Article Snippet: The dark-adapted ERG was recorded for Ccl2 − / − /Cx3cr1 gfp/gfp (LKC Technologies, Gaithersburg, MD, USA) [ ].

Techniques: Control, Comparison

Journal: Journal of Translational Medicine

Article Title: Wnt signaling in age-related macular degeneration: human macular tissue and mouse model

doi: 10.1186/s12967-015-0683-x

Figure Lengend Snippet: ERG of Ccl2 − / − /Cx3cr1 − / − /rd8 and Ccl2 − / − /Cx3cr1 gfp/gfp mice. Left panel Averaged photopic b-wave amplitudes elicited with UV and green light flashes from the treated and control eyes in Ccl2 − / − /Cx3cr1 − / − /rd8. Right panel Serial ERG responses to different intensities of white light flashes after dark adaptation were recorded in the treated and control eyes in Ccl2 − / − /Cx3cr1 gfp/gfp . Data are present as mean ± SEM of 5 mice. *: p < 0.05; **: p < 0.01. ***: p < 0.001

Article Snippet: The dark-adapted ERG was recorded for Ccl2 − / − /Cx3cr1 gfp/gfp (LKC Technologies, Gaithersburg, MD, USA) [ ].

Techniques: Control

Journal: Journal of Translational Medicine

Article Title: Wnt signaling in age-related macular degeneration: human macular tissue and mouse model

doi: 10.1186/s12967-015-0683-x

Figure Lengend Snippet: Histology of the Ccl2 − / − /Cx3cr1 − / − /rd8 mouse retina. Representative histological micrographs: fewer lesions in anti-LRP6 treated eyes, as shown by the reduced loss of photoreceptors and more organized retinal structure. Black arrows indicate degenerative photoreceptors in the OPL ( top right panel ). Red arrows show degeneration and atrophy of ONL and loss of IS/OS in mIgG-injected group ( bottom right panel ). Some Crb1-associated lesions in the OPL are also seen ( asterisk , top left panel ) (H&E, original magnification, ×200). GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, IS / OS inner segments/outer segments of photoreceptors

Article Snippet: The dark-adapted ERG was recorded for Ccl2 − / − /Cx3cr1 gfp/gfp (LKC Technologies, Gaithersburg, MD, USA) [ ].

Techniques: Injection

Journal: Journal of Translational Medicine

Article Title: Wnt signaling in age-related macular degeneration: human macular tissue and mouse model

doi: 10.1186/s12967-015-0683-x

Figure Lengend Snippet: Retinal A2E accumulation in the Ccl2 − / − /Cx3cr1 − / − /rd8 mice. Retinal A2E levels in anti-LRP6 treated and control eyes of Ccl2 − / − /Cx3cr1 − / − /rd8. A2E concentrations were determined by HPLC in the retina. The A2E level was reduced after anti-LRP6 antibody injection. n = 5 from each group; *: p < 0.05

Article Snippet: The dark-adapted ERG was recorded for Ccl2 − / − /Cx3cr1 gfp/gfp (LKC Technologies, Gaithersburg, MD, USA) [ ].

Techniques: Control, Injection

Journal: Journal of Translational Medicine

Article Title: Wnt signaling in age-related macular degeneration: human macular tissue and mouse model

doi: 10.1186/s12967-015-0683-x

Figure Lengend Snippet: mRNA expression of Wnt signaling pathway target genes in the treated and control eyes in Ccl2 − / − /Cx3cr1 − / − /rd8 mice. In general, there is lower transcript level in the eyes received anti-LRP6 compared to the control eyes measured by quantitative RT-PCR. *: p < 0.05

Article Snippet: The dark-adapted ERG was recorded for Ccl2 − / − /Cx3cr1 gfp/gfp (LKC Technologies, Gaithersburg, MD, USA) [ ].

Techniques: Expressing, Control, Quantitative RT-PCR