cwith Search Results


cwith specificantibodies against inos  (Spring Bioscience)
86
Spring Bioscience cwith specificantibodies against inos
Figure 4 e Effect of JAK/STAT inhibitors on PRV-induced ROS and inflammatory response in RAW264.7 cells. (A) IL-6 and (B) NO production was determined by ELISA and Griess reagent. RAW264.7 cells were pretreated with different concentrations of STAT-3 inhibitor (Line 1: 25 mM; Line 3: 50 mM) or JAK2 inhibitor AG490 (Line 2: 10 mM, Line 4: 20 mM), and DMSO (Line 5), or received no treatment (mock, Line 6) for 1.5 hours; the cells were then infected with PRV or left uninfected (basal, Line 7) for 24 hours. (C) Cell lysates were prepared and subjected to Western blot analysis using antibodies against <t>iNOS</t> Q25 , COX-2, and NF-kB (p50 and p65); the signals were normalized against the GAPDH. (D) RAW264.7 cells were untreated (DMSO) or pretreated with Alga (100 mM), AG490 (20 mM), and STAT-3 inhibitor (50 mM) for 1.5 hours, and then infected with PRV or left uninfected (basal). After 24 hours, the cells were stained with 10 mM of DCFH-DA for 30 minutes, and the ROS production was determined using a fluorescence microplate reader. All measurements were performed in triplicate. The results are presented as means ± SD of three independent experiments. *p < 0.01, significant compared with PRV-infected RAW264.7 cells (DMSO). Alga ¼ D. salina carotenoid extract; DMSO ¼ dimethyl sulfoxide; ELISA ¼ enzyme-linked immunosorbent assay; IL ¼ interleukin; JAK ¼ Janus kinase; NF-kB ¼ nuclear factor-kB; PRV ¼ pseudorabies virus; SD ¼ standard deviation; ROS ¼ reactive oxygen species; STAT ¼ signal transducer and activator of transcription.
Cwith Specificantibodies Against Inos, supplied by Spring Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cwith/against+cwith+inos+specificantibodies/pm28987368-127-19-23
Average 86 stars, based on 1 article reviews
cwith specificantibodies against inos - by Bioz Stars, 2026-10
86/100 stars
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streptavidin alexa 405  (Jackson Laboratory)
86
Jackson Laboratory streptavidin alexa 405
Figure 4 e Effect of JAK/STAT inhibitors on PRV-induced ROS and inflammatory response in RAW264.7 cells. (A) IL-6 and (B) NO production was determined by ELISA and Griess reagent. RAW264.7 cells were pretreated with different concentrations of STAT-3 inhibitor (Line 1: 25 mM; Line 3: 50 mM) or JAK2 inhibitor AG490 (Line 2: 10 mM, Line 4: 20 mM), and DMSO (Line 5), or received no treatment (mock, Line 6) for 1.5 hours; the cells were then infected with PRV or left uninfected (basal, Line 7) for 24 hours. (C) Cell lysates were prepared and subjected to Western blot analysis using antibodies against <t>iNOS</t> Q25 , COX-2, and NF-kB (p50 and p65); the signals were normalized against the GAPDH. (D) RAW264.7 cells were untreated (DMSO) or pretreated with Alga (100 mM), AG490 (20 mM), and STAT-3 inhibitor (50 mM) for 1.5 hours, and then infected with PRV or left uninfected (basal). After 24 hours, the cells were stained with 10 mM of DCFH-DA for 30 minutes, and the ROS production was determined using a fluorescence microplate reader. All measurements were performed in triplicate. The results are presented as means ± SD of three independent experiments. *p < 0.01, significant compared with PRV-infected RAW264.7 cells (DMSO). Alga ¼ D. salina carotenoid extract; DMSO ¼ dimethyl sulfoxide; ELISA ¼ enzyme-linked immunosorbent assay; IL ¼ interleukin; JAK ¼ Janus kinase; NF-kB ¼ nuclear factor-kB; PRV ¼ pseudorabies virus; SD ¼ standard deviation; ROS ¼ reactive oxygen species; STAT ¼ signal transducer and activator of transcription.
Streptavidin Alexa 405, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cwith/conjugated+cwith+pe+streptavidin/pmc12412662-314-18-20
Average 86 stars, based on 1 article reviews
streptavidin alexa 405 - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

Image Search Results


Figure 4 e Effect of JAK/STAT inhibitors on PRV-induced ROS and inflammatory response in RAW264.7 cells. (A) IL-6 and (B) NO production was determined by ELISA and Griess reagent. RAW264.7 cells were pretreated with different concentrations of STAT-3 inhibitor (Line 1: 25 mM; Line 3: 50 mM) or JAK2 inhibitor AG490 (Line 2: 10 mM, Line 4: 20 mM), and DMSO (Line 5), or received no treatment (mock, Line 6) for 1.5 hours; the cells were then infected with PRV or left uninfected (basal, Line 7) for 24 hours. (C) Cell lysates were prepared and subjected to Western blot analysis using antibodies against iNOS Q25 , COX-2, and NF-kB (p50 and p65); the signals were normalized against the GAPDH. (D) RAW264.7 cells were untreated (DMSO) or pretreated with Alga (100 mM), AG490 (20 mM), and STAT-3 inhibitor (50 mM) for 1.5 hours, and then infected with PRV or left uninfected (basal). After 24 hours, the cells were stained with 10 mM of DCFH-DA for 30 minutes, and the ROS production was determined using a fluorescence microplate reader. All measurements were performed in triplicate. The results are presented as means ± SD of three independent experiments. *p < 0.01, significant compared with PRV-infected RAW264.7 cells (DMSO). Alga ¼ D. salina carotenoid extract; DMSO ¼ dimethyl sulfoxide; ELISA ¼ enzyme-linked immunosorbent assay; IL ¼ interleukin; JAK ¼ Janus kinase; NF-kB ¼ nuclear factor-kB; PRV ¼ pseudorabies virus; SD ¼ standard deviation; ROS ¼ reactive oxygen species; STAT ¼ signal transducer and activator of transcription.

Journal: Journal of food and drug analysis

Article Title: Dunaliella salina alga extract inhibits the production of interleukin-6, nitric oxide, and reactive oxygen species by regulating nuclear factor-κB/Janus kinase/signal transducer and activator of transcription in virus-infected RAW264.7 cells.

doi: 10.1016/j.jfda.2016.11.018

Figure Lengend Snippet: Figure 4 e Effect of JAK/STAT inhibitors on PRV-induced ROS and inflammatory response in RAW264.7 cells. (A) IL-6 and (B) NO production was determined by ELISA and Griess reagent. RAW264.7 cells were pretreated with different concentrations of STAT-3 inhibitor (Line 1: 25 mM; Line 3: 50 mM) or JAK2 inhibitor AG490 (Line 2: 10 mM, Line 4: 20 mM), and DMSO (Line 5), or received no treatment (mock, Line 6) for 1.5 hours; the cells were then infected with PRV or left uninfected (basal, Line 7) for 24 hours. (C) Cell lysates were prepared and subjected to Western blot analysis using antibodies against iNOS Q25 , COX-2, and NF-kB (p50 and p65); the signals were normalized against the GAPDH. (D) RAW264.7 cells were untreated (DMSO) or pretreated with Alga (100 mM), AG490 (20 mM), and STAT-3 inhibitor (50 mM) for 1.5 hours, and then infected with PRV or left uninfected (basal). After 24 hours, the cells were stained with 10 mM of DCFH-DA for 30 minutes, and the ROS production was determined using a fluorescence microplate reader. All measurements were performed in triplicate. The results are presented as means ± SD of three independent experiments. *p < 0.01, significant compared with PRV-infected RAW264.7 cells (DMSO). Alga ¼ D. salina carotenoid extract; DMSO ¼ dimethyl sulfoxide; ELISA ¼ enzyme-linked immunosorbent assay; IL ¼ interleukin; JAK ¼ Janus kinase; NF-kB ¼ nuclear factor-kB; PRV ¼ pseudorabies virus; SD ¼ standard deviation; ROS ¼ reactive oxygen species; STAT ¼ signal transducer and activator of transcription.

Article Snippet: The membranes were then blocked in Tris-buffered salineeTween 20 solution containing 5% nonfat dry milk and incubatedovernight at 4 Cwith specificantibodies against iNOS (Spring Bioscience Q), COX-2 (Spring Bioscience), NF-kB p50 (Spring Bioscience), NF-kB p65 (Spring Bioscience), JAK (Santa Cruz), pJAK (Abcam), STAT-1 (Cell Signaling), pSTAT-1 (Epitomics), STAT-3 (Cell Signaling), pSTAT-3 (Cell Signaling), suppressor of cytokine signaling (SOCS) 3 (Cell Signaling), and GAPDH Q(Novus).

Techniques: Enzyme-linked Immunosorbent Assay, Infection, Western Blot, Staining, Virus, Standard Deviation

Figure 5 e Alga inhibits virus-induced activation of iNOS, COX-2, and NF-kB in RAW264.7 cells. RAW264.7 cells were untreated (DMSO) or pretreated with 100 mM of Alga for 1.5 hours, and then infected with PRV (DMSO) or left uninfected (basal). After 6e24 hours, protein levels of (A) iNOS Q26 and COX-2, and (B) NF-kB p50 and p65 in the RAW264.7 cells were assayed via Western blot analysis. All measurements were performed in triplicate. Alga ¼ D. salina carotenoid extract; DMSO ¼ dimethyl sulfoxide; NF-kB ¼ nuclear factor-kB; PRV ¼ pseudorabies virus.

Journal: Journal of food and drug analysis

Article Title: Dunaliella salina alga extract inhibits the production of interleukin-6, nitric oxide, and reactive oxygen species by regulating nuclear factor-κB/Janus kinase/signal transducer and activator of transcription in virus-infected RAW264.7 cells.

doi: 10.1016/j.jfda.2016.11.018

Figure Lengend Snippet: Figure 5 e Alga inhibits virus-induced activation of iNOS, COX-2, and NF-kB in RAW264.7 cells. RAW264.7 cells were untreated (DMSO) or pretreated with 100 mM of Alga for 1.5 hours, and then infected with PRV (DMSO) or left uninfected (basal). After 6e24 hours, protein levels of (A) iNOS Q26 and COX-2, and (B) NF-kB p50 and p65 in the RAW264.7 cells were assayed via Western blot analysis. All measurements were performed in triplicate. Alga ¼ D. salina carotenoid extract; DMSO ¼ dimethyl sulfoxide; NF-kB ¼ nuclear factor-kB; PRV ¼ pseudorabies virus.

Article Snippet: The membranes were then blocked in Tris-buffered salineeTween 20 solution containing 5% nonfat dry milk and incubatedovernight at 4 Cwith specificantibodies against iNOS (Spring Bioscience Q), COX-2 (Spring Bioscience), NF-kB p50 (Spring Bioscience), NF-kB p65 (Spring Bioscience), JAK (Santa Cruz), pJAK (Abcam), STAT-1 (Cell Signaling), pSTAT-1 (Epitomics), STAT-3 (Cell Signaling), pSTAT-3 (Cell Signaling), suppressor of cytokine signaling (SOCS) 3 (Cell Signaling), and GAPDH Q(Novus).

Techniques: Virus, Activation Assay, Infection, Western Blot