Journal: Nature protocols

Article Title: Single-virus tracking with quantum dots in live cells.

doi: 10.1038/s41596-022-00775-7

Figure Lengend Snippet: Fig. 4 | Graphics depicting the labeling strategies in QSVT experiments. a, Labeling the outer components of biotinylated viruses with SA-QDs via biotin–streptavidin interaction. b, Labeling the outer components of HyNic-modified viruses with 4FB-QDs via direct chemical coupling. c, Labeling the inner components with NPAb-QDs by electroporation followed by labeling of outer components with 4FB-QDs by direct chemical coupling to obtain dual-labeled viruses for imaging. d, Labeling the inner components with MMBs via capsid breathing motion of virus capsid and simultaneous labeling of the outer components with SA-QDs to obtain dual-labeled viruses for imaging.

Article Snippet: 601002 and 602002) ● Pasteur pipette, 3 mL (NEST, cat. no. 318212) ● 0.22 μm filter (Thermo Fisher Scientific, cat. no. 097203) ● Superdex 200 separation column (GE Healthcare) ● NAP-5 column (GE Healthcare) ● Micropipette with tips (Thermo Fisher Scientific) ● Microtiter plates, U-bottom, 96 well (Thermo Scientific, cat. no. 2205) ● Copper mesh ● Snap-cap microtubes, 1.5 mL (Eppendorf, cat. no. 022363204) ● Parafilm (Millipore, cat. no. P7793) ● Rotator (Scientz, cat. no. HS-3) ● Magnetic stirrers (Thermo Fisher Scientific, cat. no. SP88857106) ● Electroporation cuvette (Bio-Rad, cat. no. 1652082EDU) ● Spectrophotometer (Thermo Scientific Multiskan SkyHigh, cat. no. A51119600DPC) Virus culture in eggs ● Pencil ● Egg trays ● Injection syringe ● Egg candling light ● Cotton swabs ● Sterile forceps ● Egg incubator (Taisite Instrument, cat. no. SPX-70BIII)

Techniques: Labeling, Electroporation, Imaging, Virus